|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | Optimization of carotenoid production by Phaffia rhodozyma cells grown on xylose 显示文摘 | PARAJO J C SANTOS V VAZQUEZ M | 1998 | Process Biochem1998，33，2： | 1 |
| 3 | Effect of CO2 Addition to Raw Milk on Proteolysis and Lipolysis at 4℃显示文摘 | MA Y BARBANO D M SANTOS M V | 2003 | J Dairy Sci2003，86，： | 1 |
| 4 | Mechanical properties of HSLA bainitic steel subjected to controlled rolling with accelerated cooling显示文摘 | RODRIGUES P C M PERELOMA E V SANTOS D B | 2000 | Materials Science and Engineering: A2000，283，： | 1 |
| 5 | Resuscitation of Escherichia coil VBNC cells depends on a variety of environmental or chemical stimuli 显示文摘 | Pinto D Almeida V Almeida Santos M Chambel L | 2011 | Appl Microbiol2011，110，6： | 1 |
| 6 | Quantification of α-lactalbumin in human milk:method validation and application显示文摘 | Santos L H L M L M Ferreira I M L V O | 2007 | Anal Biochem2007，362，2： | 1 |
| 7 | Lactation curves for milk, fat and protein in dairy cows: a full approach显示文摘 | SILVESTRE A M MARTINS A M SANTOS V A | 2009 | Livest Sci2009，122，： | 1 |
| 8 | The impact of knowledge about diabetes, resilience and de- pression on glyeemic control: a cross-sectional study among adolescents and young adults with type 1 diabetes显示文摘 | SANTOS F R BERNARDO V GABBAY M A | 2013 | Di- abetol Metab Syndr2013，5，1： | 1 |
| 9 | The value of Cockcroft-Gault equation for the screening of decreased renal function in patients w ith systemic arterial hypertension显示文摘 | Santos E M Franca A K Salgado J V | 2011 | J Bras Nefrol2011，33，3： | 1 |
| 10 | On-line system for preconcentration and determination of metals in vegetables by inductively coupled plasma optical emission spectrometry显示文摘 | BEZERRA M A dos SANTOS W N L LEMOS V A | 2007 | Journal of Hazardous Materials2007，148，12： | 1 |
| 11 | Sequential double lung transplant in Kartagener,s syndrome显示文摘 | LAMA M R SANTOS L F SALVATIERRA V A | 2000 | Arch Bronconeumol2000，36，： | 1 |
| 12 | Design of dedicated instrumentation for temperature distribution measurements in solid oxide fuel cells显示文摘 | Adzic M Heitor M V Santos D | 1997 | Journal of Applied Electrochemistry1997，27，12： | 1 |
| 13 | Conservation of methylation reprogramming in mammalian development:aberrant reprogramming in cloned embryos显示文摘 | Dean W Santos F Stojkovic M Zakhartchenko V Walter J Wolf E Reik W | 2001 | Proceedings of the National Academy of Sciences2001，98，13： | 1 |
| 14 | The meaning of specializa- tion for the nurse in the field of stomas显示文摘 | De Paula M A Santos V L | 2003 | Rev Lat Am Enfermagem2003，11，4： | 1 |
| 15 | Oxidation of CO,ethanol and toluene over TiO2 supported noble metal catalysts显示文摘 | SANTOS V P CARABINEIRO S A C TAVARES P B PEREIRA M F R ORFAO J J M FIGUEIREDO J L | | 0，，： | 1 |
| 16 | Identification of bovine and porcine rotavirus G types by PCR显示文摘 | Gouvea V Santos N Timenetsky M | 1994 | J Clin Microbiol1994，32，： | 1 |
| 17 | Cryopreservation of Iberian red deer ( Cervus elaphus hispanicus) epididymal spermatozoa: Effects of egg yolk, glycerol and cooling rate显示文摘 | Fernandez- Santos M R Esteso M C Montoro V | 2006 | Theriogenology2006，66，： | 1 |
| 18 | Immobilization of Fe, Mn and Co tetraphenylporphyrin complexes in MCM-41 and their catalytic activity in cyclohexene oxidation reaction by hydrogen peroxide 显示文摘 | COSTA A A GHESTI G F MACEDO J L BRAGA V S SANTOS M M DIAS J A DIAS S C L | 2008 | J Mol Catal A: Chem2008，282，： | 1 |
| 19 | The K shortest paths problem显示文摘 | Martins E Q V Pascoal M M B Santos J L E | 1998 | European Journal of Operational Research1998，165，1： | 1 |
| 20 | Protection against increased intestinal permeability and bacterial translocation induced by intestinal obstruction in mice treated with viable and heat-killed Saccha- romyces boulardii显示文摘 | GENEROSO S V VIANA M L SANTOS R G | 2011 | Eur J Nntr2011，50，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员