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| 1 | Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation显示文摘AIM:To investigate whether the inhibition of autophagy by chloroquine(CQ)sensitizes rectal tumors to radiation therapy(RT)or concurrent chemoradiation(chemoRT).METHODS:In vitro,HCT-116 and HT-29 colorectal cancer(CRC)cell lines were treated as following:(1)PBS;(2)CQ;(3)5-fluorouracil(5-FU);(4)RT;(5)CQ and RT;(6)5-FU and RT;(7)CQ and 5-FU;and(8)5-FU and CQ and RT.Each group was then exposed to various doses of radiation(0-8 Gy)depending on the experiment.Cell viability and proliferative capacity were measured by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and clonogenic assays.Clonogenic survivalcurves were constructed and compared across treatment groups.Autophagy status was determined by assessing the LC3-Ⅱto LC3-Ⅰratio on western blot analysis,autophagosome formation on electron microscopy and identification of a perinuclear punctate pattern with GFPlabeled LC3 on fluorescence microscopy.Cell cycle arrest and cell death were evaluated by FACS and AnnexinⅤanalysis.All experiments were performed in triplicate and statistical analysis was performed by the student’s t test to compare means between treatment groups.RESULTS:RT(2-8 Gy)induced autophagy in HCT-116and HT-29 CRC cell lines at 4 and 6 h post-radiation,respectively,as measured by increasing LC3-Ⅱto LC3-Ⅰratio on western blot.Additionally,electron microscopy demonstrated autophagy induction in HT-29 cells24 h following irradiation at a dose of 8 Gy.Drug treatment with 5-FU(25μmol/L)induced autophagy and the combination of 5-FU and RT demonstrated synergism in autophagy induction.CQ(10μmol/L)alone and in combination with RT effectively inhibited autophagy and sensitized both HCT-116 and HT-29 cells to treatment with radiation(8 Gy;P<0.001 and 0.00001,respectively).Significant decrease in clonogenic survival was seen only in the HT-29 cell line,when CQ was combined with RT at doses of 2 and 8 Gy(P<0.5 and P=0.05,respectively).There were no differences in cell cycle progression or Annexin V staining upon CQ addition to RT.CONCLUSION:Autophagy inhibition by CQ increases CRC cell sensitivity to concurrent treatment with 5-FU and RT in vitro,suggesting that addition of CQ to chemoRT improves CRC treatment response. | Caitlin A Schonewolf Monal Mehta Devora Schiff Hao Wu Bruce G Haffty Vassiliki Karantza Salma K Jabbour | 2014 | World Journal of Gastrointestinal Oncology2014，6，3： | 12 |
| 2 | Stereoselective synthesis of 3-O-methyl-6- fluorodopa via fluorodestannylation 显示文摘 | Michael J A Jianming Lu Salma Jivan | 1994 | Journal of Labelled Compounds and Radiopharmaeeuricals1994，34，6： | 1 |
| 3 | Low temperature induces the accumulation of phenylalanine ammonia-lyase and chalcone synthase mRNAs of Arabidopsis thaliana in a light-dependent manner 显示文摘 | Leyva A Janllo J A Salmas J | 1995 | Plant physiology1995，108，1： | 1 |
| 4 | Lateral supraor- bital approach vs pterional approach:an anatomic qualita- tive and quantitative evaluation显示文摘 | Salma A Alkandari A Sammet S | 2011 | Neurosurgery2011，68，2： | 1 |
| 5 | Microscopic and endoscopic anterior communicating artery complex anatomy as seen through keyhole approaches显示文摘 | Yeremeyeva E Salma A Chow A | 2012 | J Clin Neurosci2012，19，10： | 1 |
| 6 | IFN-γ-receptor deficiency in an infant with fatal bacille calmette-guerin infection显示文摘 | Emmanuelle J Frederic A Salma L | 1996 | N Engl J Med1996，335，26： | 1 |
| 7 | Posterior interhemispheric transfalx transprecuneus approach to the atrium of the lateral ventricle: a cadaveric study显示文摘 | Wang S Salma A Ammirati M | 2010 | J Neurosurg2010，113，5： | 1 |
| 8 | Synthesis and Structure Elucidation of New Series of Organotin ( IV ) Esters with Bio-screening Activity and Catalytic Study 显示文摘 | Muhammad A Mahhoob A Salma S | 2011 | Inorg Chem Commun2011，14，： | 1 |
| 9 | Microscopic and endoscopic anterior communicating artery complex anatomy as seen through keyhole approaches显示文摘 | Yeremeyeva E Salma A Chow A | 2012 | Clin Neurosci2012，19，10： | 1 |
| 10 | Effects of hand hygiene cam- paigns on incidence of laboratory-confirmed influenza and absenteeism in sehoolchildrens, cairo, egypt 显示文摘 | MAHA T SALMA A ERICA D | 2011 | Emerg Infect Dis2011，17，4： | 1 |
| 11 | Lateral supraorbital approach vs pterional approach: an anatomic qualitative and quantitative evaluation 显示文摘 | SALMA A ALKANDARI A SAMMET S | 2011 | Neurosurgery2011，68，2： | 1 |
| 12 | Lateral supraor- bital approach vs pterional approach: an anatomic qualitative and quantitative evaluation显示文摘 | SALMA A ALKANDARI A SAMMET S | 2011 | Neurosurgery2011，68，1： | 1 |
| 13 | Posterior interhemispheric transfalx transprecuneus approach to the atrium of the lateral ventricle: a cadaveric study显示文摘 | WANG S SALMA A AMMIRATI M | 2010 | J Neurosurg2010，113，1： | 1 |
| 14 | Effect of dietary Rhodobacter capsulatus on cholesterol concentration and fatty acid composition in broiler meat 显示文摘 | SALMA U MIAH A G MAKI T | 2007 | Poultry Science2007，86，9： | 1 |
| 15 | Lateral supraorbital ap- proach vs pterional approach: an anatomic qualitative and quantita- tive evaluation 显示文摘 | Salma A Alkandari A Sammet S | 2011 | Neurosurgery2011，68，2： | 1 |
| 16 | The role of the motor system in discriminating normal and degraded speech sounds显示文摘 | D’ Ausilio A Bufalari I Salmas P | 2012 | Cortex2012，48，7： | 1 |
| 17 | Structural social capital and innovation: is knowledge transfer the missing link?显示文摘 | RAFFAELE F SALMA A | 2014 | Journal of Knowledge Management2014，18，4： | 1 |
| 18 | Suliman,Spectrofluorimetric Determination of Zinc Using 8-Hydroxy-7-(4-Sulfo-1-Naphthylazo)-5-Quinoline Sulfonic Acid显示文摘 | Salma M Z A Sheikha T A | 2008 | Spectrochimica Acta A2008，71，3： | 1 |
| 19 | Effect of dietary Rhodobacter capsulatus on cholesterol concentration and fatty acid composition in broiler meat显示文摘 | SALMA U MIAH A G MAKI T | 2007 | Poultry Science2007，86，9： | 1 |
| 20 | Effect of dietary Rhodobacter capsulatus on egg-yolk cholesterol and laying hen performance显示文摘 | SALMA U MIAH A G TAREQ K M | | Poultry Science0，86，4： | 1 |
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