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| 1 | Endogenous IGFBP‐3 regulates excess collagen expression in intestinal smooth muscle cells of Crohn’s disease strictures显示文摘 | Robert S.Flynn SunilaMahavadi Karnam S.Murthy John R.Grider John M.Kellum HomayoonAkbari John F.Kuemmerle | 2010 | Inflamm Bowel Dis2010，，1： | 1 |
| 2 | Rapamycin impairs trabecular bone acquisition from high‐dose but not low‐dose intermittent parathyroid hormone treatment显示文摘 | P.J.Niziolek S.Murthy S.N.Ellis K.B.Sukhija T.A.Hornberger C.H.Turner A.G.Robling | 2009 | J Cell Physiol2009，，3： | 1 |
| 3 | Effect of Palm Oil on Oxygen Transfer in a Stirred Tank Bioreactor显示文摘 | Suhaila Mohd Sauid Veluri V P S.Murthy | | 0，，21： | 1 |
| 4 | Development and characterization of Saccharomyces cerevisiae strains genetically modified to over-express the pentose phosphate pathway regulating transcription factor STB5 in the presence of xylose显示文摘Background Several enzymes in the pentose phosphate pathway of Saccharomyces cerevisiae have been identified as relating to the constraint of xylose consumption and conversion to ethanol.However,no strategy has been proposed for simultaneous regulation of all contributing enzymes.If multiple enzymes contribute to constraint,over expression of a native transcription factor controlling the entire constraining pathway may provide optimal pathway wide regulation.Further characterization of this strain on both pure sugars and lignocellulosic hydrolysates would provide an opportunity to identify additional bot-tlenecks not addressed by the modification of the pentose phosphate pathway expression pattern.Results A series of strains were developed expressing STB5 and PGI1 under the control of a novel xylose inducible promoter.Increased transcription of STB5 and its regulatory targets was verified via qRT-PCR.No statistically significant difference was found in terms of xylose consumption or ethanol yield in these strains versus control strains.Xylose consumption through both the fermentative and respiratory pathways appeared to be related to oxygen availability and culture density with high-density(low oxygen)cultures consuming xylose more slowly than low-density cultures.The maximum specific consumption rate for high-density cultures was 0.21 g xylose/gDCW/h versus 0.41 g xylose/gDCW/h in lower density cultures.Statistically similar ethanol yields at high and low density(approximately 0.25 g ethanol/g xylose)suggest that the maximum rate of fermentation is linked to the rate of respiration in a stoichiometric fashion.Conclusion This study did not find evidence supporting the pentose phosphate pathway constraint identified in other works.Instead,NAD+availability mediated by oxygen availability and citric acid cycle flux was suggested to limit fermentation.While increased aeration could provide increased conversion of NAD+to NADH(and a stoichiometric increase in fermen-tation flux),this increase would not be expected improve ethanol yield beyond 50%of the theoretical maximum.Based on these findings,future work in Saccharomyces cerevisiae development for fermentation of lignocellulosic hydrolysates should focus on balancing NAD+/NADH availability through non-respiratory pathways. | William Hohenschuh Ronald E.Hector Jeffrey A.Mertens Ganti S.Murthy | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 1 |
| 5 | Clinical trial: oral feeding with a soft diet compared with clear liquid diet as initial meal in mild acute pancreatitis显示文摘 | E.SATHIARAJ S.MURTHY M. J.MANSARD G. V.RAO S.MAHUKAR D. N.REDDY | 2008 | Alimentary Pharmacology & Therapeutics2008，，6： | 1 |
| 6 | Using high-throughput data and dynamic flux balance modeling techniques to identify points of constraint in xylose utilization in Saccharomyces cerevisiae显示文摘Background Several enzymes and cofactors have been identified as contributing to the slow utilization of xylose by xylose-fermenting strains of Saccharomyces cerevisiae.However,there has been no consensus on which of these possible bottle-necks are the most important to address.A previous strain characterization study from our lab suggested that insufficient NAD+limits fermentation and may be the most important bottleneck affecting utilization of xylose for the production of ethanol.The development and validation of a genome scale dynamic flux balance model would help to verify the existence and extent of this and other metabolic bottlenecks and suggest solutions to guide future strain development thereby minimiz-ing bottleneck impact on process economics.Results A dynamic flux balance model was developed to identify bottlenecks in several strains of S.cerevisiae,both with wild-type pentose phosphate pathway expression and with the pathway over expressed.ZWF1 was found to be limiting in the oxidative portion of the pentose phosphate pathway under oxygen replete conditions.This pathway is used to regenerate NADPH.Under oxygen limiting conditions,respiration of xylose was limited by the lack of oxygen as a terminal electron acceptor.Ethanol production was also limited under these conditions due to the inability to balance NAD+/NADH.The model suggests the use of the anaplerotic glyoxylate pathway to improve NAD+/NADH balance,increasing ethanol produc-tion by 50%while producing succinate as a coproduct at upwards of 20 g/l.Conclusion In the production of high value chemicals from biomass,the use of the respiratory metabolism is a waste of feedstock carbon.Bottlenecks previously identified in the oxidative pentose phosphate pathway are currently only relevant under oxygen-replete conditions and cannot impact the partitioning of carbon between the respiratory and fermentative pathways.Focusing future efforts on the non-respiratory balancing of NAD+/NADH,perhaps through the glyoxylate pathway,would improve the economics of ethanol production both directly and through coproduct formation. | William Hohenschuh Ronald E.Hector Frank Chaplen Ganti S.Murthy | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 0 |
| 7 | Bioreactor control systems in the biopharmaceutical industry:a critical perspective显示文摘Industrial-scale bioprocessing underpins much of the production of pharmaceuticals,nutraceuticals,food,and beverage processing industries of the modern world.The proftability of these processes increasingly leverages the economies of scale and scope that are critically dependent on the product yields,titers,and productivity.Most of the processes are controlled using classical control approaches and represent over 90%of the industrial controls used in bioprocessing industries.However,with the advances in the production processes,especially in the biopharmaceutical and nutraceutical industries,monitoring and control of bioprocesses such as fermentations with GMO organisms,and downstream processing has become increasingly complex and the inadequacies of the classical and some of the modern control systems techniques is becoming apparent.Therefore,with increasing research complexity,nonlinearity,and digitization in process,there has been a critical need for advanced process control that is more efective,and easier process intensifcation and product yield(both by quality and quantity)can be achieved.In this review,industrial aspects of a process and automation along with various commercial control strategies have been extensively discussed to give an insight into the future prospects of industrial development and possible new strategies for process control and automation with a special focus on the biopharmaceutical industry. | Sagnik Mitra Ganti S.Murthy | 2022 | Systems Microbiology and Biomanufacturing2022，2，1： | 0 |
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