|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Molecular Epidemiological Study on Prevalence of Human Papillomaviruses in Patients with Common Warts in Beijing Area显示文摘Objective To study the circulation,distribution,and genomic diversity of HPVs in common warts in Beijing area of China.Methods Forty eight patients with pathologically diagnosed common warts were screened for the presence of HPV with HPV type-specific PCR and direct sequencing analysis.The genomic diversity of HPVs prevalent in Chinese patients was analyzed based on LCR.Results Forty one(85.5%)samples were positive for HPV DNA,13(31.7%)-HPV-57, 12(29.3%)-HPV-1a,7(17%)-HPV-27 and 5(12.2%)HPV-2a.Four cases were infected with two different HPV types,two(4.9%) with HPV-1a and HPV-27,one(2.4%)with HPV-1 and HPV-57 and one(2.4%)with HPV-27 and HPV-57.In contrast to the prevalence of single strain of novel HPV-57 variant and HPV-1 prototype.two HPV-2 and three HPV-27 novel variants were found to circulate in Beijing.Conclusion HPV-1.-2.-27 and-57 are predominantly prevalent in patients with common warts in Beijing. | YAN-JUN LEI CHEN GAO CHEN WANG JUN HAN JIAN-MING CHEN GUANG-CAI XIANG QI SHI HUI-YING JIANG WEI ZHOU RUN AN CHEN-FANG DONG YU-KANG YUAN XIAO-PING DONG | 2009 | Biomedical and Environmental Sciences2009，22，1： | 4 |
| 2 | Expressions of Nucleoside Diphosphate Kinase (nm23) in Tumor Tissues are Related with Metastasis and Length of Survival of Patients with Hepatocellular Carcinoma显示文摘Objective To evaluate the relationship of expressions of nucleoside diphosphate kinase (nm23) and proliferating cell nuclear antigen (PCNA), as well as apoptosis, with the prognosis of HCC patients by analyzing their pathological and clinical data. Methods The expressions of nm23 and PCNA were analyzed by immunohistochemistry and the apoptotic phenomena were detected by TUNEL technique in the liver samples from 43 HCC tissues, 39 para-neoplastic tissues, and 10 normal tissues. The mean apoptosis index (AI) and proliferative index (PI) in individual sample were calculated. Results As shown by the detection, 32.6% of carcinomas had negative nm23 signal in tumor tissues, whereas all para-neoplastic and normal tissues had positive nm23. The AI in nm23 positive HCC was significantly higher than that in nm23 negative one, with statistical difference (P<0.05). Furthermore, the expressions of nm23, and the values of AI and PI were contrastively analyzed with some main pathological and clinical data of HCC. It revealed that HCC with extrahepatic metastasis showed remarkable correlation with the negative nm23 (P=0.013) and higher PI values of HCC (P=0.015). The disease-free survival in HCC patients with negative nm23 expression was significantly poorer than that in patients with positive nm23 expression. Conclusion These data suggest that expressions of nm23 protein in tumor tissues are correlated with occurrences of metastasis and length of survival of the HCC patients, which may be an indicator for their prognosis. | RUN AN JIE MENG QI SHI XIAO-XIA DAI JING-HONG CHEN YAN-JUN LEI BING SHAN CHEN GAO YONG-LIE CHU XIAO-PING DONG | 2010 | Biomedical and Environmental Sciences2010，23，4： | 4 |
| 3 | Establishment of a Sandwich ELISA Method for Detection of Vascular Endothelial Growth Factor in Serum Samples of Hepatocellular Carcinoma Patients显示文摘Objective To establish a sandwich ELISA method for detecting vascular endothelial growth factor (VEGF) in sera of population and the patients with hepatocellular carcinoma (HCC). Methods Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF proteins were expressed and purified from E. coli in His-Trx or GST fusion forms. The specific VEGF antibodies were elicited in experimental rabbits and mice by immunization of the full length VEGF fusion protein His-Trx-VEGF1-165. After purification of antibodies with chromatograph of Protein G, a sandwich ELISA technique was established. Serum VEGF levels were evaluated in 229 adults and 291 HCC patients. Results SDS-PAGE displayed that the molecular weights of the expressed full length (His-Trx-VEGF1-165), N-terminal (His-Trx-VEGF1-100) and C-terminal (GST-VEGF100-165) human VEGF fusion proteins were about 38KD, 31KD, and 33KD, respectively. Western blots confirmed that the prepared antisera were able to recognize both prokaryoticly and eukaryoticly expressed recombinant VEGF proteins. Assays of serially diluted His-Trx-VEGF1-100 by the established sandwich ELISA method showed that the linear range of the standard curve was 0.625-320 ng/mL, with the squared correlation coefficient R2=0.991. Screening of a serum panel containing 291 serum samples of HCC patients and 229 health adults revealed that the average VEGF level in HCC patients was higher than that in healthy controls, with a statically significant difference. Conclusion The established sandwich ELISA reflects the level of serum VEGF and provide scientific basis for screening metastasis and recurrence of HCC using serum VEGF as an index. | BING SHAN CHEN GAO JIAN-MING CHEN XIN-Yu BI BAO-YUN ZHANG YAN GUO CHEN-FANG DONG RUN AN QI SHI JING-QUN HU PING ZHAO JUN HAN XIAO-PING DONG | 2008 | Biomedical and Environmental Sciences2008，21，1： | 4 |
| 4 | Over-expression of nm23-H1 in HeLa cells provides cells with higher resistance to oxidative stress possibly due to raising intracellular p53 and GPX显示文摘瞄准:决定反肿瘤因素 nm23 是否与反氧化是相关的。方法:全身的人的 nm23-H1 被克隆进哺乳动物表示的向量并且短暂地介绍了房间进 HeLa。结果:反应的氧种类(ROS ) 显著地低级的 A 在细胞被检测过去表示的 nm23-H1。3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyltetrazolium 溴化物和尝试平底锅蓝色试金发现有一个 nm23-H1-expressing 原生质标志的房间 transfected 有更高的生存能力和更强壮的抵抗到氧化应力。Immunoprecipitation 测试表明内长的 nm23-H1 与 p53 形成了蛋白质建筑群。而且, p53 的细胞内部的层次和 p53-regulatedgene GPX1 显然在表示 nm23-H1 上在房间被增加。下面在在表示 nm23-H1 上的房间的 p53 的规定导致了更高细胞的 ROS 水平和更低的房间生存能力。结论:调查结果建议 nm23-H1 可以对氧化应力充当一个细胞的保护者,可能触发 p53 相关的抗氧化的小径。 | Run AN Yong-lie CHU Chan TIAN Xiao-xia DAI Jing-hong CHEN Qi SHI Jun HAN Xiao-ping DONG | 2008 | Acta Pharmacologica Sinica2008，29，12： | 2 |
| 5 | Soft tissue release combined with joint-sparing osteotomy for treatment of cavovarus foot deformity in older children: Analysis of 21 cases显示文摘BACKGROUND Cavovarus foot is a common form of foot deformity in children,which is clinically characterized by an abnormal increase of the longitudinal arch of the foot,and it can be simultaneously complicated with forefoot pronation and varus,rearfoot varus,Achilles tendon contracture,or cock-up toe deformity.Muscle force imbalance is the primary cause of such deformity.Many diseases can lead to muscle force imbalance,such as tethered cord syndrome,cerebral palsy,Charcot-Marie-Tooth disease,and trauma.At present,many surgical treatments are available for cavovarus foot.For older children,priority should be given to midfoot osteotomy and fusion.Since complications such as abnormal foot length,foot stiffness,and abnormal gait tend to develop postoperatively,it is important to preserve the joints and correct the deformity as much as possible.Adequate soft tissue release and muscle balance are the keys to correcting the deformity and avoiding its postoperative recurrence.AIM To assess the efficacy of soft tissue release combined with joint-sparing osteotomy in the treatment of cavovarus foot deformity in older children.METHODS The clinical data of 21 older children with cavovarus foot deformity(28 feet)who were treated surgically at the Ninth Department of Orthopedics of Jizhong Energy Xingtai Mining Group General Hospital from November 2014 to July 2017 were retrospectively analyzed.The patients ranged in age from 10 to 14 years old,with an average age of 12.46±1.20 years.Their main clinical manifestations were deformity,pain,and gait abnormality.The patients underwent magnetic resonance imaging of the lumbar spine,electromyographic examination,weightbearing anteroposterior and lateral X-rays of the feet,and the Coleman block test.Surgical procedures including metatarsal fascia release,Achilles tendon or medial gastrocnemius lengthening,'V'-shaped osteotomy on the dorsal side of the metatarsal base,opening medial cuneiform wedge osteotomy,closing cuboid osteotomy,anterior transfer of the posterior tibial tendon,peroneus longus-tobrevis transfer,and calcaneal sliding osteotomy to correct hindfoot varus deformity were performed.After surgery,long leg plaster casts were applied,the plaster casts were removed 6 wk later,Kirschner wires were removed,and functional exercise was initiated.The patients began weight-bearing walk 3 mo after surgery.Therapeutic effects were evaluated using the Wicart grading system,and Meary’s angles and Hibbs’angles were measured based on X-ray images obtained preoperatively and at last follow-up to assess their changes.RESULTS The patients were followed for 6 to 32 mo,with an average follow-up period of 17.68±6.290 mo.Bone healing at the osteotomy site was achieved at 3 mo in all cases.According to the Wicart grading system,very good results were achieved in 18 feet,good in 7,and fair in 3,with a very good/good rate of 89.3%.At last follow-up,mean Meary’s angle was 6.36°±1.810°,and mean Hibbs’angle was 160.21°±4.167°,both of which were significantly improved compared with preoperative values(24.11°±2.948°and 135.86°±5.345°,respectively;P<0.001 for both).No complications such as infection,skin necrosis,or bone nonunion occurred.CONCLUSION Soft tissue release combined with joint-sparing osteotomy has appreciated efficacy in the treatment of cavovarus foot deformity in older children. | Zhen-Yu Chen Zhan-Yong Wu Yue-Hui An Li-Fei Dong Jia He Run Chen | 2019 | World Journal of Clinical Cases2019，7，20： | 2 |
| 6 | Machine learning for detecting mesial temporal lobe epilepsy by structural and functional neuroimaging显示文摘Mesial temporal lobe epilepsy(mTLE),the most common type of focal epilepsy,is associated with functional and structural brain alterations.Machine learning(ML)techniques have been successfully used in discriminating mTLE from healthy controls.However,either functional or structural neuroimaging data are mostly used separately as input,and the opportunity to combine both has not been exploited yet.We conducted a multimodal ML study based on functional and structural neuroimaging measures.We enrolled 37 patients with left mTLE,37 patients with right mTLE,and 74 healthy controls and trained a support vector ML model to distinguish them by using each measure and the combinations of the measures.For each single measure,we obtained a mean accuracy of 74%and 69%for discriminating left mTLE and right mTLE from controls,respectively,and 64%when all patients were combined.We achieved an accuracy of 78%by integrating functional data and 79%by integrating structural data for left mTLE,and the highest accuracy of 84%was obtained when all functional and structural measures were combined.These findings suggest that combining multimodal measures within a single model is a promising direction for improving the classification of individual patients with mTLE. | Baiwan Zhou Dongmei An Fenglai Xiao Running Niu Wenbin Li Wei Li Xin Tong Graham J Kemp Dong Zhou Qiyong Gong Du Lei | 2020 | Frontiers of Medicine2020，14，5： | 2 |
| 7 | Kinetic Characteristics of Neutralizing Antibody Responses Vary among Patients with COVID-19显示文摘Objective The coronavirus disease 2019(COVID-19) pandemic continues to present a major challenge to public health. Vaccine development requires an understanding of the kinetics of neutralizing antibody(NAb) responses to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods In total, 605 serum samples from 125 COVID-19 patients(from January 1 to March 14, 2020)varying in age, sex, severity of symptoms, and presence of underlying diseases were collected, and antibody titers were measured using a micro-neutralization assay with wild-type SARS-CoV-2.Results NAbs were detectable approximately 10 days post-onset(dpo) of symptoms and peaked at approximately 20 dpo. The NAb levels were slightly higher in young males and severe cases, while no significant difference was observed for the other classifications. In follow-up cases, the NAb titer had increased or stabilized in 18 cases, whereas it had decreased in 26 cases, and in one case NAbs were undetectable at the end of our observation. Although a decreasing trend in NAb titer was observed in many cases, the NAb level was generally still protective.Conclusion We demonstrated that NAb levels vary among all categories of COVID-19 patients. Longterm studies are needed to determine the longevity and protective efficiency of NAbs induced by SARS-CoV-2. | LI Ling Hua TU Hong Wei LIANG Dan WEN Chun Yan LI An An LIN Wei Yin HU Ke Qi HONG Wen Shan LI Yue Ping SU Juan ZHAO San Tao LI Wei YUAN Run Yu ZHOU Ping Ping HU Feng Yu TANG Xiao Ping KE Chang Wen KE Bi Xia CAI Wei Ping | 2021 | Biomedical and Environmental Sciences2021，34，12： | 1 |
| 8 | A No- vel Kernel-based Fuzzy C-Means Algorithm with Spatial Information for Image Segmentation显示文摘 | Yang Yue Guo Shuxu Tian Run/an | 2011 | En- ergy Procedia2011，13，： | 1 |
| 9 | Jordan Semi-Triple Multiplicative Maps on the Hermitian Matrices显示文摘In this paper,we show that every injective Jordan semi-triple multiplicative map on the Hermitian matrices must be surjective,and hence is a Jordan ring isomorphism. | Si Qing YAN Run Ling AN Jin Chuan HOU | 2011 | Journal of Mathematical Research and Exposition2011，31，6： | 1 |
| 10 | A Characterization of *-Automorphism on B(H)显示文摘<正> Let H be a Hilbert space and ■ be a standard *-subalgebra of B(H). We show that abijective map φ: ■→■ preserves the Lie-skew product AB - BA~* if and only if there is a unitaryor conjugate unitary operator U ■B(H) such that φ(A) = UAU~* for all A∈■ , that is, φ is a linear*-isomorphism or a conjugate linear *-isomorphism. | Run Ling AN Jin Chuan HOU | 2010 | Acta Mathematica Sinica,English Series2010，26，2： | 1 |
| 11 | Equivalent Characterization of Centralizers on B(H)显示文摘Let H be a Hilbert space with dim H≥2 and Z∈B(H) be an arbitrary but fixed operator.In this paper we show that an additive map Φ:B(H)→B(H) satisfies Φ(AB)=Φ(A)B=AΦ(B)for any A,B∈B(H) with AB=Z if and only if Φ(AB)=Φ(A)B=AΦ(B),A,B ∈B(H),that is,Φ is a centralizer.Similar results are obtained for Hilbert space nest algebras.In addition,we show that Φ(A^2)=AΦ(A)=Φ(A)A for any A∈B(H) with A^2=0 if and only if Φ(A)=AΦ(I)=Φ(I)A,A∈B(H),and generalize main results in Linear Algebra and its Application,450,243–249(2014) to infinite dimensional case.New equivalent characterization of centralizers on B(H) is obtained. | Wen Si XU Run Ling AN Jin Chuan HOU | 2016 | Acta Mathematica Sinica,English Series2016，32，9： | 1 |
| 12 | SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF AN AMINO ACID MODIFIED PHTHALOCYANINE显示文摘SYNTHESISANDSPECTROSCOPICPROPERTIESOFANAMINOACIDMODIFIEDPHTHALOCYANINE￥RunAnDONG;WongQIU;XinQiSONG(DepartmentofChemistry,Tsin... | Run An DONG Wong QIU Xin Qi SONG (Department of Chemistry, Tsinghua University, Beijing 100084)De Wen CHEN (Institute of Chemisty The Chinese Academy of Science, Beijing 100080) | 1996 | Chinese Chemical Letters1996，7，5： | 0 |
| 13 | Photo-induced reactions of sulfonated aluminum phthalocyanine with peptide显示文摘The photosensitized oxidation mechanism of peptide in the presence of the CIAIPcS is studied by UV-Vis spectroscopic method. It is found that the oxidation of peptide is totally quenched in 1.1 ×10-2 mol/L NaN3 solution, and the same reaction cannot proceed in the dark. K3Fe(CN)6 and L-Cys cannot quench the reaction. Therefore, the reaction mechanism is mainly Foote’s type Ⅱ. This reaction process can be inferred as follows: ( ⅰ ) active oxygen can attack acylamida bond near by carbon atom; ( ⅱ ) carboxyl group departs from peptide; ( ⅲ ) l-benzazole cycle is destroyed when reaction is continued; ( Ⅳ ) the peptide is completely damaged. | Run’an Dong Yong Qiu Xinqi Song | 1999 | Chinese Science Bulletin1999，44，5： | 0 |
| 14 | RASSF1A methylation as a biomarker for detection of colorectal cancer and hepatocellular carcinoma显示文摘BACKGROUND Studies have validated the potential of methylated cell-free DNA as a biomarker in various tumors,and methylated DNA in plasma may be a potential biomarker for cancer.AIM To evaluate the diagnostic value of RASSF1A methylation in plasma for colorectal cancer(CRC)and hepatocellular carcinoma(HCC).METHODS A total of 92 CRC patients,67 colorectal polyp(CRP)patients,63 HCC patients,and 66 liver cirrhosis(LC)patients were enrolled.The plasma DNA was subjected to DNA extraction,double-strand DNA concentration determination,bisulfite conversion,purification,single-strand DNA concentration determination,and digital polymerase chain reaction(PCR)detection.The methylation rate was calculated.The diagnostic value was evaluated by the area under the curve(AUC).RESULTS The age and sex in the CRC and CRP groups and the HCC and LC groups were also matched.The DNA methylation rate of RASSF1A in plasma in the CRC group was 2.87±1.80,and that in the CRP group was 1.50±0.64.DNA methylation of RASSF1A in plasma showed a significant difference between the CRC and CRP groups.The AUC of RASSF1A methylation for discriminating the CRC and CRP groups was 0.82(0.76-0.88).The AUCs of T1,T2,T3 and T4 CRC and CRP were 0.83(0.72-0.95),0.87(0.78-0.95),0.86(0.77-0.95),and 0.75(0.64-0.85),respectively.The DNA methylation rate of RASSF1A in plasma in the HCC group was 4.45±2.93,and that in the LC group was 2.46±2.07.DNA methylation of RASSF1A in plasma for the HCC and LC groups showed a significant difference.The AUC of RASSF1A methylation for discriminating the HCC and LC groups was 0.70(0.60-0.79).The AUCs of T1,T2,T3 and T4 HCC and LC were 0.80(0.61,1.00),0.74(0.59-0.88),0.60(0.42-0.79),and 0.68(0.53-0.82),respectively.CONCLUSION RASSF1A methylation in plasma detected by digital PCR may be a potential biomarker for CRC and HCC. | Jian Li Huan Li Zeng-Ci Run Zhen-Lei Wang Tao Jiang Yang An Zhi Li | 2022 | World Journal of Gastrointestinal Oncology2022，14，8： | 0 |
| 15 | Photo-responsive nanoparticles for β-lapachone delivery in vitro显示文摘Drug efficiency delivery and release to target site play an important role in the treatment of cancer. To achieve these goals, developing drug delivery systems is the key step. The common materials used for drug delivery like liposomes, micelles, dendrimers cannot control the delivery and releasing by spatial and temporal. In this paper, we design a photoresponsive drug delivery system to reduce the side effects of traditional chemotherapy on normal cells and improve therapeutic efficacy in treating cancers. We synthesized two kinds of photo-responsive polymers using two different monomers containing two photoresponsive groups. The two monomers were crosslinked by poly(ethylene glycol) dimethacrylate to form polymers and then encapsulated b-lapachone into the nanoparticles, after UV irradiation, the particle will degrade and release the drug. This method may provide new strategies for the photoresponsive nanomaterials. | Huibo Li Changmai Chen Qin An Guoyan Huo Mingtao Run | 2018 | Chinese Chemical Letters2018，29，9： | 0 |
| 16 | Efficient Isomerization for Preparing thans-2,2-Dimethyl-3-(2,2-dichloroethenyl) cyclopropanecarbonyl Chloride显示文摘Trans-2,2-dimethyl-3-(2,2-dichloroethenyl)cyclopropanecarbonyl chloride, which is animportant intermediate for insecticides, is obtained by isomerization of the cis/trans-mixture with4- (N,N-dimethylamino) pyridine (DMAP) in the presence or absence of halogen compounds ingood yield. | Jun An MA Run Qiu HUANG Jun Ran CHENG Rui Lian SHAO Zai Guo LI(Institute and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin300071) | 1999 | Chinese Chemical Letters1999，10，2： | 0 |
| 17 | Size-refinement enhanced flexibility and electrochemical performance of MXene electrodes for flexible waterproof supercapacitors显示文摘Increasing mechanical flexibility without sacrificing electrochemical performance of the electrode material is highly desired in the design of flexible electrochemical energy storage devices.In metal-related materials science,decreasing the grain size introduces more grain boundaries;this stops dislocations and crack propagation under deformation,and results in increased strength and toughness.However,such a size refinement effect has not been considered in the mechanical properties,particle stacking,wetting,and electrochemical performances of flexible supercapacitor electrodes.In this paper,MXene was used as an electrode material to study the size refinement effect of flexible supercapacitors.Size refinement improved the strength and toughness of the MXene electrodes,and this resulted in increased flexibility.Finite elemental analysis provided a theoretical understanding of size refinement-increased flexibility.Moreover,the size refinement also improved the specific surface area,electric conductance,ion transportation,and water wetting properties of the electrode,and the size refinement provided highly increased energy density and power density of the MXene supercapacitors.A highly flexible,water-proof supercapacitor was fabricated using size-refined MXene.The current study provides a new viewpoint for designing tough and flexible energy storage electrodes.The size refinement effect may also be applicable for metal ion batteries and electronic and photo devices composed of MXene and other nanoparticles. | Jinkun Sun Yingjian Liu Jiayi Huang Jiatian Li Mengmeng Chen Xiaoyu Hu Yatao Liu Run Wang Yanan Shen Jingjing Li Xuecheng Chen Dong Qian Baigang An Zunfeng Liu | 2021 | Journal of Energy Chemistry2021，30，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员