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| 1 | Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development. | Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun | 2020 | Molecular Plant2020，13，8： | 11 |
| 2 | Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples显示文摘瞄准:比较胰腺的腺癌织物标本的基因表示侧面,胰腺的人和腺癌和白血病房间线和正常的胰取样以便区分差别的冒号表示了基因并且在内视镜的指导超声的好针渴望( 指导EUS 的 FNA )由量的即时 RT-PCR ( RT-QPCR )验证基因的一个子集的微分表示标本。方法:包含 1176 基因的商业地奉献的癌症 cDNA 宏数组(地图集人癌症 1.2 ) 被使用。不同统计途径(层次聚类的、主要部件分析(PCA ) 和 SAM ) 被用来分析表示数据。RT-QPCR 和免疫组织化学的研究被用于结果的确认。结果:RT-QPCR 验证了 LCN2 的增加的表示(lipocalin 2 ) 并且第一次在恶意的胰的小块地(织物类型 plasminogen 使活跃之物或 tPA ) 同样与正常相比胰。Immunohistochemical 分析证实了在癌入侵的管的上皮细胞局部性的 LCN2 蛋白质的增加的表示。在与胰腺的腺癌从病人通过指导 EUS 的 FNA 获得的 12 件样品的小块地和 LCN2 抄本的分析与在正常纸巾发现的那些比较显示出显著地增加的表示层次,显示高质量的 RNA 的足够的数量能与这种技术被获得。结论:表示介绍是一个有用方法识别简历标记和潜在的目标基因。在胰腺的癌症的指导 EUS 的 FNA 样品的分子的分析为胰腺的腺癌的诊断作为珍贵策略出现。 | Henrik Laurell Michèle Bouisson Philippe Berthelémy Philippe Rochaix Sébastien Déjean Philippe Besse Christiana Susini Lucien Pradayrol Nicole Vaysse Louis Buscail | 2006 | World Journal of Gastroenterology2006，12，21： | 5 |
| 3 | Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution显示文摘We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5(PGR5)capable of producing hydrogen(H2) for 25 days with more than 30-fold yield increase compared to wild type.Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species(ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photooxidative damage and thereby ensures electron supply to the hydrogenase. | Mei Chen Jin Zhang Lei Zhao Jiale Xing Lianwei Peng Tingyun Kuang Jean-David Rochaix Fang Huang | 2016 | Journal of Integrative Plant Biology2016，58，12： | 2 |
| 4 | 理性设计拢牛儿物牛儿基焦磷酸合酶(GGPPS)能够显著增强模式植物烟草类胡萝卜合成及光合作用效率显示文摘植物品种培育的关键是寻找优良基因.通过理性设计对植物关键酶进行改造,有望为植物品种培有提供新的优良基因.NtGGPPSI是烟草类胡萝卜素合成途径的限速酶,ntggppsl突变体类胡萝卜含量显著降低.对NtGGPPS1进行理性设计,重塑酶与底物的结合口袋,构建单点饱和突变体文库.通过高通量的细菌颜色实验进行筛选,获.得5个可以显著提升酶活性的氨基酸位点,进一步对以上位点进行迭代组合突变获得酶活性最高的5突变体d-NtGGPPS1(V154A/1161L/F218Y/1209S/V233E).相对于NtGGPPSI,理性设计的d-NtGGPPSI可以将底物IPP和共同底物DMAPP转化为GGPP的效率提高约1995.5倍,能够将GPP或FPP转化产生GGPP的效率提高约25.9或16.7倍.将d-NtGGPPSI回补到ntggppsI突变体可以显著提升叶片类胡萝卜含量,生物量及光合作用效率.该研究表明理性设计GGPPS可以提升植物的产量和品质性状,并为未来通过理性设计获得植物优良基因提供新的突破口. | 董臣 曲戈 郭敬功 位芳 高书文 孙周通 金立锋 孙旭武 Jean-David Rochaix 苗雨晨 王燃 | 2022 | Science Bulletin2022，67，3： | 2 |
| 5 | Regulation and dynamics of the light- harvesting system 显示文摘 | Rochaix J D | 2014 | Annu Rev Plant Biol2014，65，4： | 1 |
| 6 | A chloroplast gene is required for the light-independent accumulation of chlorophyll in Chlamydomnoans reinhartii显示文摘 | Choquet Y Rahire M Girard-Bascou J Erickson J and Rochaix J-D | 1992 | EMBO1992，11，： | 1 |
| 7 | Regulation and dynamics of the light-harvesting system 显示文摘 | Rochaix J D | 2014 | Annual Review of Plant Biology2014，65，： | 1 |
| 8 | The AF-1 activation-function of ERalpha may be dispensable to mediate the effect of estradiol on endothelial NO production in mice 显示文摘 | Pendaries C Darblade B Rochaix P | 2002 | Prec Natl Acad Sci USA2002，99，： | 1 |
| 9 | Bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas显示文摘 | Stevens D Rochaix J Purton S | 1996 | Mol Gen Genet1996，251，： | 1 |
| 10 | National authority for health:France显示文摘 | Rochaix L Xerri B | | 0，，： | 1 |
| 11 | Somatostatin receptor subtype 2 sensitizes human pancreatic cancer cells to death ligand induced apoptosis显示文摘 | Julie Guillermet Nathalie Saint - Laurent Philippe Rochaix | 2003 | Cell Biology2003，100，1： | 1 |
| 12 | Somatostatin receptor subtype 2 sensitizes human pancreatic cancer cells to death ligand-induced apoptosis显示文摘 | Guillermet J Saint-Laurent N Rochaix P | 2003 | Proc Nat| Acad Sci USA2003，100，1： | 1 |
| 13 | In vivo patterns of Bcl-2 family protein expression in breast carcinomas in relation to apoptosis显示文摘 | Rochaix P Krajewski S Reed JC | 1999 | J Pathol1999，187，4： | 1 |
| 14 | Somatostatin receptor subtype 2 sensitizes human pancreatic cancer cells to death ligand-induced apoptosis 显示文摘 | Guillerrnet J Saint-Laurent N Rochaix P | 2003 | Proc Natl Acad Sci U S A2003，100，1： | 1 |
| 15 | Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genes显示文摘 | Dron M Rahire M Rochaix J D | 1982 | Journal of Molecular Biology1982，162，： | 1 |
| 16 | Gene therapy for pancreatic carcinoma: local and distant antitumor effects after somatostatin receptor sst2 gene transfer显示文摘 | Rochaix P Delesque N Esteve JP | 1999 | Hum Gene Ther1999，10，6： | 1 |
| 17 | Reduced expression of retinoic acid receptor beta protein (RAR beta) in human papillary thyroid carcinoma: immunohistochemical and Western blot study 显示文摘 | Rochaix P Monteil - Onteniente S Rochette - Egly C | 1998 | Histopathology1998，33，4： | 1 |
| 18 | Post-transcriptional regulation of chloroplast gene expression in Chlamydomonas reinhardtii显示文摘 | J.-D. Rochaix | 1996 | Plant Molecular Biology (-)1996，，1： | 1 |
| 19 | Gene therapy for pancreatic carcinoma: local and distant antitumor effects after somatostatin receptor sst2 gene transfer显示文摘 | Rochaix P Delesque N Esteve JP | 1999 | Human Gene Therapy1999，10，6： | 1 |
| 20 | Information Asymmetry and Search in the Market for Physicians' Services显示文摘 | Rochaix L | 1989 | Journal of Health Economics1989，3，8： | 1 |
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