|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 肿瘤坏死因子-α和白细胞介素-1β对人骨关节炎软骨细胞蛋白聚糖代谢的影响显示文摘目的通过体外培养骨关节炎(OA)软骨细胞体系,了解肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β对蛋白聚糖代谢的调节作用。方法软骨细胞来自OA患者进行膝关节置换术的股骨髁.实验采用第1代软骨细胞。随机将细胞分为4组:对照组、TNF-α组、IL-1β组和联合组(TNF—α+IL-1β),在培养液中加入不同的刺激因子共培养4d。采用3种方法了解蛋白聚糖的代谢水平:①用二甲基亚甲蓝分光光度法(DMMB法)检测细胞中及培养液中的糖胺多糖(GAG)含量;②酶联免疫吸附法通过2种抗蛋白聚糖单克隆抗体(Mab-383和5D4)检测培养液中蛋白聚糖的不同代谢片段;③反转录-聚合酶链式反应(RT—PCR)半定量法检测软骨细胞的蛋白聚糖、带有血小板凝血酶敏感蛋白样模体的解整链蛋白金属蛋白酶-4(ADAMTS-4)和ADAMTS-5的mRNA水平。结果①实验组培养液中GAG含量均明显高于对照组(P〈0.01);②TNF-α组、IL-1β组及联合组的培养液中5D4片段的水平均高于对照组(P〈0.01),而各组383片段水平差异无统计学意义;③实验组的蛋白聚糖mRNA水平均低于对照组(P〈0.01),TNF—α组的ADAMTS-5、IL-1β组的ADAMTS-4及联合组的两种酶的mRNA水平均显著高于对照组(P〈0.01)。结论TNF-α及IL-1β可下调入OA软骨细胞的蛋白聚糖mRNA表达,上调ADAMTS-4及ADAMTS-5的mRNA表达,并促进蛋白聚糖的分解,可能在OA的发病中起着一定的作用。 | 周惠琼 张奉春 Rob K Will | 2008 | 中华风湿病学杂志2008，12，2： | 7 |
| 2 | Environmental Cost-Benefit Analysis of Alternative Timing Strategies in Greenhouse Gas Abatement: A Data Environment Analysis Approaeh显示文摘 | TIMO K NIEL B ROB D | 2009 | Ecological Economics2009，68，6： | 1 |
| 3 | Cueing for Schema Construction: Designing Problem-solving Multimedia Practicals 显示文摘 | Hans G K Hummel Rob J Nadolski | 2002 | Contemporary Educational Psychology2002，，27： | 1 |
| 4 | Oral combination therapy with a nucleoside polymerase inhibitor (RG7128) and danoprevir for chronic hepatitis C genotype 1 infection (INFORM-1): a randomised, double-blind, placebo-controlled, dose-escalation trial显示文摘 | Edward J Gane Stuart K Roberts Catherine AM Stedman Peter W Angus Brett Ritchie Rob Elston David Ipe Peter N Morcos Linda Baher Isabel Najera Tom Chu Uri Lopatin M Michelle Berrey William Bradford Mark Laughlin Nancy S Shulman Patrick F Smith | 2010 | The Lancet2010，，9751： | 1 |
| 5 | Significance of acute multiple brain infarction on diffusion-weighted imaging 显示文摘 | Rob J K Kang D W Lee S H | 2000 | Stroke2000，31，3： | 1 |
| 6 | End-group modification of poly(butyl acrylate) prepared by atom transfer radical polymerisation:mechanistic study using gradient polymer elution chromatography显示文摘 | Auke S Bert K Rob V | 2002 | J Polym Sci Part A:Polym Chem2002，40，： | 1 |
| 7 | Cyclosporin Derivatives Inhibit Interleukin 1β Induction of Nitric Oxide Synthase in Renal Mesangial Cells显示文摘 | Heiko M Dieter K Rob P | 1993 | Eur J Pharmacology1993，249，12： | 1 |
| 8 | Liver X receptor mediates hepatitis B virus X protein-induced lipogenesis in hepatitis B virus-associated hepatocellular carcinoma 显示文摘 | Na TY Shin YK Rob K J | 2009 | Hepatology2009，49，4： | 1 |
| 9 | Staging of brain pathology related to sporadic Parklnson's disease显示文摘 | Braak H Del Tredici K ROb U | 2003 | Neurobiol Aging2003，24，2： | 1 |
| 10 | Therapeutic potential of mesenchymal stromal cells in a,breast cancer metastasis model显示文摘 | Sun B Rob K H Park J R | 2009 | Cytotherapy2009，11，3： | 1 |
| 11 | Development of a Rapid Quantitative PCR Assay for Direct Detection and Quantification of Culmrable and Non-culmrable Escherichia coil from Agriculture watersheds显示文摘 | IZHAR U H VIC G ROB K | 2007 | Journal of Microbiological Methods2007，69，： | 1 |
| 12 | Assessing anonymous communication on the Internet: policy deliberations 显示文摘 | ROB K YACHING L MARK S F | 1999 | Information Society1999，，2： | 1 |
| 13 | Performance-driven simul- taneous placement and routing for FPGA' s 显示文摘 | Nag Sudip K Rutenbar Rob A | 1998 | IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems1998，17，6： | 1 |
| 14 | Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology 显示文摘 | TJEERD G K LISETTE A C ROB J M | 2009 | Vaccine2009，27，28： | 1 |
| 15 | Herbivore-induced ethylene suppresses a direct defense but not a putative indirect defense against an adapted herbivore显示文摘 | Johannes Kahl David H. Siemens Rob J. Aerts Ralph G?bler Frank Kühnemann Catherine A. Preston Ian T. Baldwin | 2000 | Planta2000，，2： | 1 |
| 16 | Acute effects of the designer drugs benzylpiperazine(BZP)and tnfluoromethylphenylpiperazine(TFMPP)using functional magnetic resonance imaging(fMRI)and the Stroop task-a pilot study显示文摘 | LOUISE E C ROB R K MICHELLE C R | 2015 | Psychopharmacology2015，，： | 1 |
| 17 | Corporate Social and Environ- mental Reporting: A Review of the Literature and a Longitudinal Study of UK Disclosure 显示文摘 | ROB G RAZA K | 1995 | Account- ing Auditing & Accountability Journal1995，8，2： | 1 |
| 18 | Intuitionistic fuzzy LI- ideals in lattice implication algebras显示文摘 | Y B Jtm Y Xu K Y Qin E H Rob | 2000 | Science China Mathe- matics2000，3，2： | 1 |
| 19 | Challenges for porcine reproductive and respiratory syndrome virus(PRRSV)vaccinology显示文摘 | Tjeerd G K Lisette A C Rob J M | 2009 | Vaccine Volume2009，28，27： | 1 |
| 20 | British Association of Criti cal CareNurses position statement on the use of restraint in a dult critical careunits显示文摘 | Bray K Hill K Rob son W | 2004 | Nurs Crit Care2004，9，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员