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| 1 | Rational design of glycerol dehydratase:Swapping the genes encoding the subunitsof glycerol dehydratase to improve enzymatic properties显示文摘1,3-propanediol (1,3-PD) is an important material for chemical industry,and there has been always much interest in the production of 1,3-PD using all possible routes. The genes encoding glyc-erol dehydratase (GDHt) from Citrobacter freundii,Klebsiella pneumoniae and metagenome were cloned and expressed in E. coli. All glycerol dehy-dratases but the one from metagenome could be detected to show enzyme activities. In order to im-prove the enzymatic properties of GDHts,the genes encoding α and β-γ subunits were cloned,and the enzyme characteristics were evolved by rational de-sign based on their 3D structures which were con-structed by homology modeling. Six heteroenzymes were obtained by swapping the α subunit genes of these three different-source-derived GDHts. The pH,thermal stability and Vmax of some heteroenzymes were dramatically improved by 2―5 times compared with the wild one (GDHtKP). The GDHt cloned from metagenome,originally proved to be with no enzyme activity,was converted into active enzyme by swap-ping its subunits with other different GDHts. In addi-tion,the effect of subtle 3D structural changes on the properties of the enzyme was also observed. | QI Xianghui SUN Liang LUO Zhaofei WU Jiequn MENG Xiaolei TANG Yue WEI Yutuo HUANG Ribo | 2006 | Chinese Science Bulletin2006，51，24： | 4 |
| 2 | Expression and characterization of a novel highly glucose-tolerant [3-glucosidase from a soil metagenome显示文摘 | Jian Lu Liqin Du Yutuo Wei Yuanyuan Hu Ribo Huang | 2013 | Acta Biochimica et Biophysica Sinica2013，45，8： | 4 |
| 3 | First spaceborne demonstration of BeiDou-3 signals for GNSS reflectometry from CYGNSS constellation显示文摘The full constellation of Chinese Global Navigation Satellite System(GNSS)Bei Dou-3 has been deployed completely and started fully operational service.In addition to providing global Positioning,Navigation and Timing(PNT)services,the Bei Dou-3 satellites transmissions can also be used as the sources of illumination for Earth Observation(EO)with a bistatic radar configuration.This innovative EO concept,known as GNSS reflectometry(GNSS-R),allows to measure the Earth surface characteristics at high resolution via the reflected L-band radar signals collected by a constellation of small,low cost and low Earth orbiting satellites.For the first time in orbit,earth reflected Bei Dou-3 signal has been detected from the limited sets of raw data collected by the NASA’s Cyclone GNSS(CYGNSS)constellation.The feasibility of spaceborne Bei Dou-3 reflections on two typical applications,including sea surface wind and flooding inundation detection,has been demonstrated.The methodology and results give new strength to the prospect of new spaceborne GNSS-R instruments and missions,which can make multi-GNSS reflectometry observations available to better capture rapidly changing weather systems at better spatio-temporal scales. | Weiqiang LI Estel CARDELLACH Serni RIBO Antonio RIUS Bo ZHOU | 2021 | Chinese Journal of Aeronautics2021，34，9： | 2 |
| 4 | Thrombin-activable fibrinolysis inhibitor levels in the acute phase of ischemic stroke显示文摘 | RIBO M MONASTERIO J | 2003 | Stroke2003，34，4： | 1 |
| 5 | Admission fibrinolytic profile is associated with symptomatic hemorrhagic transformation in stroke patients treated with tissue plasminogen activator 显示文摘 | Ribo M Montaner J Molina CA al | 2004 | Stroke2004，35，9： | 1 |
| 6 | Intra-arterial administration of microbubbles and continuous 2-MHz ultrasound insonation to enhance intra-arterial thrombolysis显示文摘 | Ribo M Molina CA Alvarez B | 2010 | J Neuroimaging2010，20，3： | 1 |
| 7 | Photobacterium phosphoreum toxicity bioassay:(Ⅰ) test procedures and applications显示文摘 | RIBO J M KAISER K L E | 2006 | Toxicity Assessment2006，2，3： | 1 |
| 8 | A comparison of three uncertainty calculi for building sonar-based occupancy grids显示文摘 | Miguel Ribo Axel Pinz | 2001 | Robotics and Autonomous Systems2001，35，34： | 1 |
| 9 | Microbubble administration accelerates clot lysis during continuous 2-MHz ultrasound monitoring in stroke patients treated with intravenous tissue plasminogen activator显示文摘 | Molina C Ribo M Rubiera M | 2006 | Stroke2006，37，： | 1 |
| 10 | Tandem internal carotid artery/middle cerebral artery occlusion:an independent predictor of poor outcome after systemic thrombolysis显示文摘 | Rubiera M Ribo M Delgado-Mederos R | 2006 | Stroke2006，37，： | 1 |
| 11 | Ultraearly hematoma growthpredicts poor outcome after acute intracerebral hemorrhage显示文摘 | Rodriguez LD Rubiera M Ribo M | 2011 | Neu-rology2011，77，17： | 1 |
| 12 | Impact of admission hyperglycemia on stroke outcome after thrombolysis:Risk stratification in relation to time to reperfusion显示文摘 | Alvarez-Sabin J Molina CA Ribo M | 2004 | Stroke2004，35，： | 1 |
| 13 | Extending the time window for endovaseular procedures according to collateral pial circulation 显示文摘 | Ribo M Flores A Rubiera M | 2011 | Stroke2011，42，12： | 1 |
| 14 | Microbubble administration accelerates clot lysis during continuous 2-MHz ultrasound monitoring in stroke patients treated with intravenous tissue plasminogen activator显示文摘 | Molina CA Ribo M Rubiera M | 2006 | Stroke2006，37，： | 1 |
| 15 | Ultraerly hematoma growth predicts poor outcome after acute intracerebral hemorrhage显示文摘 | Rodriguez-Luna D Rubiera M Ribo M | | 0，，： | 1 |
| 16 | Intra-arterial administration of microbubbles and continuous 2-MHz ultrasound insonation to enhance intra-arterial thrombolysis显示文摘 | Ribo M Molina CA Alvarez B | 2010 | J Neuroimaging2010，20，3： | 1 |
| 17 | Extending the time window for endovascular procedures according to collateral pial circulation显示文摘 | Ribo M Flores A Rubiera M | | 0，，12： | 1 |
| 18 | Tandem internal carotid artery/middle cerebral artery occlusion:An independent predictor of poor outcome after systemic thrombolysis显示文摘 | Rubiera M Ribo M Delgado-Mederos R | 2006 | Stroke2006，37，： | 1 |
| 19 | Safety and efficacy of ultrasound-enhanced thrombolysis:a comprehensive review and meta-analysis of randomized and nonrandomized studies显示文摘 | Tsivgoulis G Eggers J Ribo M | | 0，，02： | 1 |
| 20 | Acute hyperglycemia state is as- sociated with lower tpa-induced xecanalization rates in stroke patients 显示文摘 | Ribo M Molina C Montaner J | 2005 | Stroke2005，36，： | 1 |
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