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| 1 | Bioactive hydrogel microcapsules for guiding stem cell fate decisions by release and reloading of growth factors显示文摘Human pluripotent stem cells(hPSC)hold considerable promise as a source of adult cells for treatment of diseases ranging from diabetes to liver failure.Some of the challenges that limit the clinical/translational impact of hPSCs are high cost and difficulty in scaling-up of existing differentiation protocols.In this paper,we sought to address these challenges through the development of bioactive microcapsules.A co-axial flow focusing microfluidic device was used to encapsulate hPSCs in microcapsules comprised of an aqueous core and a hydrogel shell.Importantly,the shell contained heparin moieties for growth factor(GF)binding and release.The aqueous core enabled rapid aggregation of hPSCs into 3D spheroids while the bioactive hydrogel shell was used to load inductive cues driving pluripotency maintenance and endodermal differentiation.Specifically,we demonstrated that one-time,1 h long loading of pluripotency signals,fibroblast growth factor(FGF)-2 and transforming growth factor(TGF)-β1,into bioactive microcapsules was sufficient to induce and maintain pluripotency of hPSCs over the course of 5 days at levels similar to or better than a standard protocol with soluble GFs.Furthermore,stem cell-carrying microcapsules that previously contained pluripotency signals could be reloaded with an endodermal cue,Nodal,resulting in higher levels of endodermal markers compared to stem cells differentiated in a standard protocol.Overall,bioactive heparin-containing core-shell microcapsules decreased GF usage five-fold while improving stem cell phenotype and are well suited for 3D cultivation of hPSCs. | Kihak Gwon Hye Jin Hong Alan M.Gonzalez-Suarez Michael Q.Slama Daheui Choi Jinkee Hong Harihara Baskaran Gulnaz Stybayeva Quinn P.Peterson Alexander Revzin | 2022 | Bioactive Materials2022，7，9： | 3 |
| 2 | Doppler sonographic criteria for the diagnosis of inferior mesenteric arterystenosis显示文摘 | PELLERITO J S REVZIN M V TSANG J C | 2009 | J Ultrasound Med2009，28，： | 1 |
| 3 | Gel electrophoresis assays for DNA-protein interactions显示文摘 | | 1989 | Biotechniques1989，7，： | 1 |
| 4 | A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions:application to components of the Escherichia coli lactose operon regulatory system显示文摘 | Revzin A | 1981 | Nucleic Acids Res1981，9，： | 1 |
| 5 | Functional imaging of neuron-astrocyte interactions in a compartmentalized microfluidic device显示文摘Traditional approaches in cultivating neural cells in a dish without orienting their interactions have had only limited success in revealing neural network properties.To enhance the experimental capabilities of studying neural circuitry in vitro,we designed an experimental system combining concepts of micropatterned surfaces,microfluidic devices and genetically encoded biosensors.Micropatterning was used to position neurons and astrocytes in defined locations and guide interactions between the two cell types.Microfluidic chambers were placed atop micropatterned surfaces to allow delivery of different pharmacological agents or viral vectors to the desired cell types.In this device,astrocytes and neurons communicated through grooves molded into the floor of the microfluidic device.By combining microfluidics with genetically encoded calcium indicators as functional readouts,we further demonstrated the utility of this device for analyzing neuron–neuron and neuron–astrocyte interactions in vitro under both healthy and pathophysiological conditions.We found that both spontaneous and evoked calcium dynamics in astrocytes can be modulated by interactions with neurons.In the future,we foresee employing the microdevices described here for studying mechanisms of neurological disorders. | Yandong Gao Joey Broussard Amranul Haque Alexander Revzin Tian Lin | 2016 | Microsystems & Nanoengineering2016，2，1： | 1 |
| 6 | Influence of alloying on the solubility of carbon fibers in aluminium-based composites : non-empirical approach 显示文摘 | REVZIN B FUKS D PELLEG J | 1996 | Composites Science and Technology1996，56，1： | 1 |
| 7 | Development of a microfabricated cytometry platform for characterization and sorting of individual leukocytes显示文摘 | Revzin A Sekine K Sin A | 2005 | Lab Chip2005，5，1： | 1 |
| 8 | Development of a micro-fabricated cytometry platform for characterization and sorting of individual leukocytes显示文摘 | REVZIN A SEKINE K SIN A | 2005 | Lab Chip2005，5，1： | 1 |
| 9 | The effec-tiveness of using controlled power turbines in gas-turbineunits and regenerative-cycle and simpJe-cycle engines显示文摘 | Komarov O V Revzin B S Brodov Yu M | 2006 | Thermal Engineering2006，53，2： | 1 |
| 10 | Some Characteristics of Wide0field Units in the Brain of the Pigeon显示文摘 | | 1970 | Brain Behaviour and Evolution1970，333，： | 1 |
| 11 | Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithgraphically patterned gold microelectrodes 显示文摘 | Revzin AF Sirkar K Simonian A | 2002 | Sens Actuators B2002，81，： | 1 |
| 12 | Glucose and lactate biosensors based on redox polymer/oxidoreductase nanocomposite thin films显示文摘 | Kaushik Sirkar Alexander Revzin Michael V Pishko | 2000 | Anal Chem2000，72，： | 1 |
| 13 | Glucose,lactate,and pyruvate biosensor arrays based on redox polymer/oxidoreductase nonocomposite thin-films deposited on photolitho-graphically patterned gold microelectrodes 显示文摘 | Revzin A F Sirkar K Simonian A | 2002 | Sensor and Actuators B2002，81，： | 1 |
| 14 | The effectiveness of using controlled power turbines in gas-turbine units and regenerative-cycle and simple-cycle engines显示文摘 | Komarov O V Revzin B S Brodov Yu M | 2006 | Thermal Engineering2006，53，2： | 1 |
| 15 | A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions:application to components of the Escherichia coli lactose operon regulatory system显示文摘 | REVZIN A | 1981 | Nucleic Acids Res1981，9，： | 1 |
| 16 | Doppler sonographic criteria for the diagnosis of inferior mesenteric artery stenosis显示文摘 | Pellerito JS Revzin MV Tsang JC | | 0，，： | 1 |
| 17 | 查看详情显示文摘 | Yan J Pedrosa V A Simonian A L Revzin A | | 0，，03： | 1 |
| 18 | A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies显示文摘There is increasing interest in utilizing in vitro cultures as patient avatars to develop personalized treatment for cancer.Typical cultures utilize Matrigel-coated plates and media to promote the proliferation of cancer cells as spheroids or tumor explants.However,standard culture conditions operate in large volumes and require a high concentration of cancer cells to initiate this process.Other limitations include variability in the ability to successfully establish a stable line and inconsistency in the dimensions of these microcancers for in vivo drug response measurements.This paper explored the utility of microfluidics in the cultivation of cancer cell spheroids.Six patient-derived xenograft(PDX)tumors of high-grade serous ovarian cancer were used as the source material to demonstrate that viability and epithelial marker expression in the microfluidic cultures was superior to that of Matrigel or large volume 3D cultures.To further demonstrate the potential for miniaturization and multiplexing,we fabricated multichamber microfluidic devices with integrated microvalves to enable serial seeding of several chambers followed by parallel testing of several drug concentrations.These valve-enabled microfluidic devices permitted the formation of spheroids and testing of seven drug concentrations with as few as 100,000 cancer cells per device.Overall,we demonstrate the feasibility of maintaining difficul-to-culture primary cancer cells and testing drugs in a microfluidic device.This microfluidic platform may be ideal for drug testing and personalized therapy when tumor material is limited,such as following the acquisition of biopsy specimens obtained by fine-needle aspiration. | Neda Dadgar Alan M.Gonzalez-Suarez Pouria Fattahi Xiaonan Hou John S.Weroha Alexandre Gaspar-Maia Gulnaz Stybayeva Alexander Revzin | 2020 | Microsystems & Nanoengineering2020，6，1： | 1 |
| 19 | Unit responses to visual stimuli in the nucleus rotundus of the pigeon显示文摘 | 7,Revzin A M | 1976 | Fed Proc1976，26，： | 1 |
| 20 | Some characteristics of wide-fields units in the brain of the pigeon显示文摘 | 8,Revzin A M | 1970 | Brain Behav Evol1970，3，： | 1 |
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