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| 1 | 南天山:晚古生代还是三叠纪碰撞造山带?显示文摘伊犁-哈萨克斯坦板块和塔里木-卡拉库姆板块之间的南天山造山带是‘中亚型造山带’的典型代表之一,经历了复杂的构造演化与地壳增生过程。传统上,它被视为华力西期褶皱带或晚古生代碰撞造山带。但近年来,部分学者提出它可能为三叠纪碰撞造山带。本文在综述南天山造山带的蛇绿岩、高压变质岩、花岗岩类等方面研究成果的基础上,讨论了其碰撞造山的时限。我国境内南天山西段碰撞造山可能开始于早石炭世(345Ma),结束于晚石炭世末(300Ma左右)。二叠纪时期,南天山至整个中亚地区进入后碰撞演化阶段。现有资料证实南天山为一晚古生代碰撞造山带,并非一三叠纪碰撞造山带。 | 高俊 龙灵利 钱青 黄德志 苏文 Reiner KLEMD | 2006 | 岩石学报2006，22，5： | 226 |
| 2 | CFD-simulation of a circulating fluidized bed riser显示文摘In the current work,a model of the fluid mechanics in the riser of a circulating fluidized bed(CFB)has been implemented using computational fluid dynamics(CFD).The model developed shall be used in future as the basis of 3D-reactor model for the simulation of large scale CFB combustors.The two-fluid model(TFM)approach is used to represent the fluid mechanics involved in the flow.The computational implementation is accomplished by the commercial software FLUENT.Different closure formulations are tested on a simplified geometry.Two different turbulence formulations,namely the swirl modified RNG k–εmodel and the Realizable k–εmodel,are tested in combination with two different approaches to solid phase turbulence,namely the dispersion and per phase approach.One focus of the current work is put on the study of different drag correlations.Besides the drag correlations by Syamlal et al.[Syamlal,M.,Rogers, W.,&O’Brien,T.J.(1993).MFIX documentation theory guide.Technical Report DOE/METC-94/1004,U.S. Department of Energy(DOE).Morgantown Energy Technology Center:Morgantown,WV]and Gidaspow [Gidaspow,D.(1994).Multiphase flow and fluidization.New York:Academic Press]the EMMS model has been used to determine the momentum exchange between the two phases.The resulting formulation is then used to simulate a 1-m×0.3-m cold CFB setup and is validated by experimental results[Schlichthrle, P.(2000).Fluid dynamics and mixing of solids and gas in the bottom zone of circulating fluidized beds. Unpublished doctoral dissertation,Technische Universitaet Hamburg-Harburg,Shaker Verlag:Aachen]. | Ernst-Ulrich Hartge Lars Ratschow Reiner Wischnewski Joachim Werther | 2009 | Particuology2009，7，4： | 39 |
| 3 | 那拉提山北缘寒武纪玄武岩的元素地球化学特征及构造意义显示文摘我国西南天山地区那拉提北缘断裂北侧出露寒武纪(516.3±7.4Ma)橄榄拉斑玄武岩,其 Al_2O_3(12.71%~14.14%)、P_2O_5(0.09%~0.21%)含量较低,TiO_2(1.39%~2.35%)、MgO(5.35%~8.14%)含量中等,全 Fe_2O_3(11.74%~16.03%)含量较高,球粒陨石标准化稀土分布型式平坦,(La/Sm)_N 和(Y/Nb)_N 比值接近1,为过渡型洋脊玄武岩(T-MORB);并且 Th/Ta 比值(0.8~1.5)低,高场强元素(Nb,Ta,Zr,Hf,P)没有明显亏损,缺乏陆壳混染或俯冲带流体活动的印迹。部分 T-MORB 的 Fe、Ti 含量较高(FeO^T>12%,TiO_2>2%,FeO^T/MgO>1.75),Mg~#值(0.40—0.49)和 Cr(53×10^(-6)~110×10^(-6))含量较低,属于 Fe-Ti 玄武岩(ferrobasalt),应为岩浆依 Fenner 趋势发生较高程度结晶分离演化的产物,很可能形成于洋中脊的扩展型裂谷环境。我们认为,那拉提北缘寒武纪 T-MORB 和 Fe-Ti 玄武岩可能与境外吉尔吉斯斯坦境内北天山南缘的早古生代 Terskey 蛇绿岩带相连,为 Terskey 蛇绿岩的残片;寒武纪我国境内发育 Terskey 洋。那拉提北缘断裂可能与尼古拉耶夫线相连,代表西南天山地区 Terskey 洋闭合形成的一条早古生代缝合带。 | 钱青 徐守礼 何国琦 Reiner KLEMD 熊贤明 龙灵利 高俊 | 2007 | 岩石学报2007，23，7： | 24 |
| 4 | Late Cretaceous-Cenozoic exhumation history of Tiantangzhai region of Dabieshan Orogen: Constraints from (U-Th)/He and fission track analysis显示文摘Integrated fission track and (U-Th)/He analysis is carried out on 6 apatite and 6 zircon samples from a near vertical section in The Tiantangzhai region at the core of the present Dabieshan orogen. The result shows that the region experienced cooling/exhumation during the Late Cretaceous and Early Tertiary period. Age-elevation relationships for different dating systems and different minerals suggest a pulse of rapid exhumation at ~110 Ma before present, preserved in the structurally highest samples. At lower elevations, ages begin to decrease with decreasing elevation, suggesting lower exhumation rates since 90 Ma. Two periods of different exhumation rates are identified since 90 Ma. The average apparent exhumation rate for the period of 43.4-22.5 is 0.062 km/Ma, whereas that for the period of 76.4-47.4 Ma is 0.039 km/Ma. | ZHOU Zuyi XU Changhai P. W. Reiners YANG Fengli R. A. Donelick | 2003 | Chinese Science Bulletin2003，48，11： | 20 |
| 5 | (U-Th)/He年龄在沉积盆地构造—热演化研究中的应用——以塔里木盆地KQ1井为例显示文摘(U-Th)/He热定年技术是近年来用于沉积盆地热史研究的新技术,目前主要是利用磷灰石和锆石的He年龄来揭示地层的构造抬升和热历史.本文依据塔里木盆地钻井样品的实测磷灰石和锆石(U-Th)/He年龄数据.初步得出了该地区磷灰石(U-Th)/He年龄的封闭温度为85℃,并建立了深度/温度—年龄演化模式;锆石则未达到其较高的封闭温度.综合利用本次实测的He年龄数据结合磷灰石裂变径迹和等效镜质组反射率等古温标,模拟计算了塔里木盆地孔雀1井(KQ1)自奥陶纪末期以来的热历史.模拟结果表明,孔雀1井区奥陶纪末期的地温梯度可达35.5℃/km,志留纪—泥盆纪时期的地温梯度为33.3~34.5℃/km,白垩纪末期地温梯度27.6℃/km左右.因此,(U-Th)/He年龄结合其他古温标综合模拟的方法可以很好地揭示沉积盆地的热历史.特别是该技术为缺乏常规古温标的塔里木盆地下古生界碳酸盐岩层系所经受热史的恢复提供了新的方法. | 邱楠生 Peter Reiners 梅庆华 姜光 Nicolesco Stefan 陶成 | 2009 | 地球物理学报2009，52，7： | 19 |
| 6 | Organochlorine pesticides in soils under different land usage in the Taihu Lake region, China显示文摘地研究在泰胡·莱克区域被进行,到在在在 year1983.Thirteen organochlorine 杀虫剂( OCP )的这些物质的禁令以后的土壤的 revealthe organochlorine 杀虫剂集中的在 2004 的中国从稻地在土壤被分析,树土地 andfallow land.Total organochlorine 杀虫剂残余比 inuncultivated 休闲土地 soils.Among 在农业土壤是更高的所有杀虫剂,西格马 DDX ( DDD , DDE 和 DDT )为所有土壤样品有 highestconcentration ,变化 | WANG Fang JIANG Xin BIAN Yong-rong YAO Fen-xia GAO Hong-jian YU Gui-fen Jean Charles MUNCH Reiner SCHROLL | 2007 | Journal of Environmental Sciences2007，19，5： | 19 |
| 7 | Methane Production, Emission and Possible Control Measures in the Rice Agriculture显示文摘In the rice field methane is produced in the soil layer with depths of 2-25 cm. The vertical profile of methane production rate in the paddy soil during the water covering period differs from that in the paddy soil in dry phase. Only a small part, about 30%. of the produced methane is emitted to the atmosphere through rice plant, air bubbles, and molecular diffusion. Therefore, the methane emission rate from the rice field depends not only on the methane production rate in the soil, but also on the transport efficiency of the rice plant, air bubble formation that in turn depends on the production rate, and molecular diffusion.Field measurements show that methane emission rates from a particular rice field have very large diurnal, seasonal and interannual variations, which are related to soil characteristics, water regime, farming procedure, local climate, and rice growing activities. The relationship between the methane emission rate and the above mentioned factors is very complicated. The emission | 王明星 上官行健 沈壬兴 Wassmann Reiner Seiler Wolfgang | 1993 | Advances in Atmospheric Sciences1993，10，3： | 14 |
| 8 | Apatite-fission-track geochronology and its tectonic correlation in the Dabieshan orogen,central China显示文摘AFT data of granitoid and metamorphic samples from the Dabieshan orogen have an age range between 96.4 Ma and 41.9 Ma coupled with confined track lengths of 11.5—14.0 μm, reflecting the cooling time of rocks differentially through the 100℃± isotherm. The Juras- sic-Eogene sediments from the North Huaiyang and Hefei basin, however, yield their AFT ages of 128.8—62.0 Ma with mean track lengths of 8.6—11.9 μm, recording a complicated thermal de- velopment of the basin. These AFT data structurally allowing the coupling between the orogen and the basin are to a great extent considered as the result from the control of NNE-trending regional tectonics. It took place approximately at 95—90 Ma that the orogen developed with an abrupt shift from the doming extension to the differential transtension, which is assumed to be related with the changes of the convergence between the Western Pacific and Eurasia plates. The differentially sinistral strike-slipping from the transtension between Tanlu and Shang- cheng-Macheng faults brought the doming extension of the orogen shrunk sharply in Late Cre- taceous. In Eogene, the full-grown transtension that dominated in East Asia was caused mainly by a rapid decrease of the rates of the Western Pacific convergence, which can be therefore re- garded as a major mechanism creating the zonal anomalies of 60—40 Ma in the orogen. The thermal anomaly of 70—40 Ma zonally along the Tanlu fault cooled more slowly than the anomaly of 60—55 Ma zoning along the Shangcheng-Macheng fault, but the 60—40 Ma anomaly trending E-W in hinterlands of the orogen seems to be relicts of the doming extension as a result of inten- sive rebuilding by the transtension. In addition, the orogen was also reactivated by the far-field effect from India-Asia collision in Eogene, where major NWW-trending faults played an important role in facilitating such far-field tectonic transferring. In Oligocene to Miocene, the Dabieshan orogen remained a few thermal disturbances with their AFT ages particularly in coincidence with the time of opening and closing of the South China Sea. | P. Van Den Haute R. A. Donelick J. De Grave MA Changqian P. W. Reiners | 2005 | Science China Earth Sciences2005，48，4： | 9 |
| 9 | Pathological bacterial translocation in liver cirrhosis显示文摘 | Reiner Wiest Melissa Lawson Markus Geuking | 2014 | Journal of Hepatology2014，，1： | 8 |
| 10 | Analysis of monocyte chemotactic protein-1 gene polymorphism in patients with spontaneous bacterial peritonitis显示文摘AIM:To investigate a genetic polymorphism of the monocyte chemotactic protein-1 (MCP-1 ) gene in patients with spontaneous bacterial peritonitis (SBP).METHODS:MCP-1 genotyping was performed in 23 patients with SBP and 83 cirrhotic control patients with non-infected ascites.RESULTS:The frequency of carriers of the G-allele was lower in SBP patients but this difference did not reach statistical significance. However,in the subgroup of patients with alcoholic cirrhosis (n=80),carriers of the G-allele were significantly less frequent in SBP-patients (38.1%) than in cirrhotic controls (67.8%,P=0.021). CONCLUSION:In patients with alcoholic liver cirrhosis,the-2518 MCP-1 genotype AA is a risk factor for the development of SBP. | Erwin Gbele Marcus Mhlbauer Hartwig Paulo Monika Johann Christin Meltzer Franz Leidl Norbert Wodarz Reiner Wiest Jrgen Schlmerich Claus Hellerbrand | 2009 | World Journal of Gastroenterology2009，15，44： | 7 |
| 11 | Ultrasonographic imaging of inflammatory bowel disease in pediatric patients显示文摘Inflammatory bowel disease(IBD) is one of the most common chronic gastrointestinal diseases in pediatric patients.Choosing the optimal imaging modality for the assessment of gastrointestinal disease in pediatric patients can be challenging.The invasiveness and patient acceptance,the radiation exposure and the quality performance of the diagnostic test need to be considered.By reviewing the literature regarding imaging in inflammatory bowel disease the value of ultrasound in the clinical management of pediatric patients is highlighted.Transabdominal ultrasound is a useful,noninvasive method for the initial diagnosis of IBD in children;it also provides guidance for therapeutic decisions and helps to characterize and predict the course of the disease in individual patients.Ultrasound techniques including color Doppler imaging and contrast-enhanced ultrasound are promising imaging tools to determine disease activity and complications.Comparative studies between different imaging methods are needed. | Liliana Chiorean Dagmar Schreiber-Dietrich Barbara Braden Xin-Wu Cui Reiner Buchhorn Jian-Min Chang Christoph F Dietrich | 2015 | World Journal of Gastroenterology2015，21，17： | 7 |
| 12 | Geochronological constraints on 140-85 Ma thermal doming extension in the Dabie orogen, central China显示文摘Regional architecture of geochronology and differential cooling pattern show that the Dabie orogen underwent a thermal doming extension during 140-85 Ma. This extension resulted in widespread re-melting of the Dabie basement, intense volcanic activities in North Huaiyang and the formation of fault-controlled depressions in the Hefei basin. This thermal doming extension can be further divided into two consecutive evolving stages, i.e. the intensifying stage (140-105 Ma) and the declining stage (105-85 Ma). In the first stage (140-105 Ma), the thermal doming mainly was concentrated in the Dabie block, and to a less degree, in the Hongan block. The thermal doming structure of the Dabie block is configured with Macheng-Yuexi thermal axis, Yuexi/Luotian thermal cores and their downslide flanks. The orientation of thermal axis is dominantly parallel to the strike of orogen, and UHP/HP units together with metamorphic rocks of North Huaiyang constitute the downslide flanks. The Yuexi core differs from the Luotian core in both the intensity and the shaping time. To some extent, the Hongan block can be regarded as part of downslide systems of the Dabie doming structure. The doming process is characterized by thermal-center's migration along the Macheng-Yuexi thermal axis; consequently, it is speculated to be attributed to the convective removal of thickened orogenic root, which is a process characterized by intermittance, migration, large-scale and differentiation. During the declining stage (105-85 Ma), the dome- shaped figure still structurally existed in the Dabie orogen, but orogenic units cooled remarkably slow and magmatic activities stagnated gradually. Study on the thermal doming of Dabieshan Mountains can thus provide detailed constraints on the major tectonic problems such as the UHP/HP exhumation model, the boundary between North Dabie and South Dabie, and the orogenesis mechanism. | 许长海 周祖翼 马昌前 Peter W. Reiners | 2002 | Science China Earth Sciences2002，45，9： | 6 |
| 13 | Origin of the deep fluids in the paleosubduction zones in western Tianshan:Evidence from Pb-and Sr-isotope compositions of high-pressure veins and host rocks显示文摘Fluids in the deep subduction zones play an important role in crust-mantle ex-change related to the subduction process. Identification of fluids origin, internal or external, can help us to evaluate the contribution of crust materials to mantle during this process. High-pressure veins, which developed in the western Tianshan HP-metamorphic belt extensively, are the direct products of the fluids in subduction zones. In western Tianshan, high-pressure veins and host rocks have overall Pb- and Sr-isotope compositions. At t = 340 Ma, the high-pressure metamorphic time, the ratios of 206Pb/204Pb, 207Pb/204Pb, 208Pb/204Pb and 87Sr/86Sr of high pressure veins are 17.122―18.431, 15.477―15.611, 37.432―38.689 and 0.70529―0.70705, and are 17.605―17.834, 15.508―15.564, 37.080―38.145 and 0.70522―0.70685 for host rocks. However, high-pressure veins show a much larger variation in Pb-isotope composi-tions than host rocks. Plots of samples are distributed roughly along a line parallel to and far away from the Northern Hemisphere reference line (NHRL) on the plot of 206Pb/204Pb vs 207Pb/204Pb. On the plot of 206Pb/204Pb vs 208Pb/204Pb, plots of data are distributed nearby mid-ocean-ridge basalts (MORB) area or along a line parallel to and not far away from NHRL, which shows that the data plots are farther away from MORB and ocean island basalts (OIB) areas on the plot of 206Pb/204Pb vs 207Pb/204Pb than on the plot of 206Pb/204Pb vs 208Pb/204Pb. The compositions of Pb- and Sr-isotopes of these high-pressure metamorphic rocks are between that of enriched mantle 1(EMI) and of sediments or upper crust. A notable characteristic can be seen from plots of Pb-isotope ratio vs Pb-isotope ratio and 206Pb/204Pb vs 87Sr/86Sr that array of plots shows an evident tendency of mixture of two end members. Ratios of Rb/Ba, Ce/Pb, Nb/U and Ta/U of high-pressure veins and host rocks are between that of OIB or MORB and that of conti-nental crust, which indicates that continental crust materials probably have been incorporated into the rocks. The fluids in the paleosubduction zones in western Tianshan are mixtures of two sources, one originating from the devolatilzation of the host rocks with protolithes similar to ocean basalts from enriched mantle 1 (EMI), the other from the dehydration of subducted sediments. | Reiner Klemd | 2005 | Science China Earth Sciences2005，48，10： | 6 |
| 14 | 俯冲板片中微量元素的活动性:西天山榴辉岩相运输脉的制约显示文摘西天山的基性高压变质岩显示互相连接的榴辉岩相脉体网络来源于蓝片岩的进变质脱水作用,通过这些网脉可以洞察在俯冲带高压条件下远程流体流动中的流体一岩石相互作用和元素负载。岩相学证据表明外来流体的渗透作用产生运输脉,而流体持续的沟道式流动导致主岩蓝片岩的淋滤。脉和蓝片岩蚀变带中锂(Li)的含量几乎是蓝片岩主岩中锂的两倍,它支持流体是外部来源的设想。这些流体触发了蓝片岩主岩的榴辉岩化作用而形成蓝片岩蚀变带。由于成脉流体中的微量元素含量低,所以导致与流体发生化学反应的主岩中所有微量元素的强烈淋滤。在此过程中有53%~81%的微量元素被活化,与大离子亲石元素和轻稀土元素的损失相符合,它们的损失量几乎是重稀土元素和高场强元素损失量的两倍。 | 熊贤明 Timm JOHN 高俊 Reiner KLEMD 黄德志 | 2006 | 地质学报2006，80，1： | 6 |
| 15 | NOBOX is a key FOXL2 partner involved in ovarian folliculogenesis显示文摘 | Justine Bouilly1,2, Reiner A. Veitia3,4 Nadine Binart1,2,* | 2014 | Journal of Molecular Cell Biology2014，8，2： | 5 |
| 16 | 俯冲洋岛玄武岩脱水释放的水流体成分:来自西天山榴辉岩相高压脉的约束显示文摘俯冲洋壳的脱水作用对于岛弧火山岩的成因具有重要意义。西天山高压低温变质带中切割主岩蓝片岩/榴辉岩的榴辉岩相脉体记录了古生代俯冲带中的脱水过程。脉体具有典型高压矿物组合:石榴石(>8%)+绿辉石(15%)+石英(>55%)+蓝闪石(5%)+冻蓝闪石(10%)±黝帘石±方解石。高压脉及其主岩的岩相学与矿物化学研究表明流体是在俯冲过程中蓝片岩向榴辉岩进变质条件下释放。脉的主量元素成分显示流体富Si。主岩的稀土元素和微量元素表明其原岩为洋岛玄武岩(OIB)。脉和主岩具有相似的微量元素配分模式,并且相对N-MORB都显示富Li、Be、Cs、Rb、Ba、Pb、La的特点。通过模拟计算,在高压脉、与脉平衡的流体以及模拟的原生流体中都富集流体活动性元素(Li、Be、Pb)和LILE(Cs、Rb、Ba),贫HFSE(Nb)和REE(Nd、Sm)。证实了在古生代南天山洋俯冲时洋岛玄武岩脱水释放出富Li、Be、LILE、La和Pb,贫HFSE和HREE的流体。 | 熊贤明 高俊 Reiner KLEMD 黄德志 | 2006 | 岩石学报2006，22，1： | 5 |
| 17 | Simulation-supported measurements in large circulating fluidized bed combustors显示文摘The concept of simulation-supported measurement is suggested for the elucidation of processes occurring in the combustion chambers of large-scale circulating fluidized bed combustors where the desired information cannot be obtained by direct measurements.The concept is illustrated with the example of secondary air injection where the way the air is released,the penetration depth and the evenness of air distribution over the cross-sectional area of the combustion chamber are of interest.The measured information consists of lateral profiles of oxygen concentrations measured with gas sampling probes at two ports which were located 5 and 9 m,respectively,above the level of secondary air injection.The simulation is carried out on the basis of a 3D semi-empirical fluid-mechanical model of the circulating fluidized bed which is combined with models of gas and solids mixing,fuel distribution,devolatilization and combustion of char and volatiles.The combination of the simulation with the measurements yields a clear picture of the mechanism of secondary air injection,its penetration into the combustion chamber and its effect on the local combustion processes.The results confirm the usefulness of the concept of simulation-supported measurement for this application. | Joachim Werther Ernst-Ulrich Hartge Lars Ratschow Reiner Wischnewski | 2009 | Particuology2009，7，4： | 5 |
| 18 | Reactive gas-solids flows in large volumes—3D modeling of industrial circulating fluidized bed combustors显示文摘A model is presented for the simulation of reactive gas-solids flows in large industrial reactors. Circulating fluidized bed (CFB) combustors with several thousands of cubic meters reaction volume are probably the largest reactors of this type. A semi-empirical modeling approach has been chosen to model the three-dimensional concentration distributions of gas and solids components and temperatures inside the combustion chamber of such boilers. Two industrial CFB boilers are investigated in detail: the 105 MWe Duisburg combustor in Germany and the 235 MWe Turów combustor in Poland. The semi-empirical model approach is described first. Then the model is used to show how the three-dimensional concentration and temperature fields are formed by the interaction of several local phenomena. Good agreement between simulation and measurements has been achieved. | Reiner Wischnewski Lars Ratschow Ernst-Ulrich Hartge Joachim Werther | 2010 | Particuology2010，8，1： | 5 |
| 19 | Heme oxygenase-1 overexpression increases liver injury after bile duct ligation in rats显示文摘AIM: To investigate the effects of heme oxygenase-1 (HO-1) against oxidant-induced injury caused by bile duct ligation (BDL). METHODS: Either cobalt protoporphyrin (CoPP), a HO-1 inducer, or saline were injected intraperitoneally in male SD-rats. Three days later, BDL or sham-operations were performed. Rats were sacrificed 3 wk after BDL and livers were harvested for histology. Fibrosis was evaluated by sirius red staining and image analysis. Alpha-smooth muscular actin, which indicates activation of stellate cells, was detected by immunohistochemical staining, and cytokine and collagen-Ⅰα (Col-Ⅰα) mRNA expression was detected using RNase protection assays. RESULTS: Serum alanine transaminase increased 8-fold above normal levels one day after BDL. Surprisingly, enzyme release was not reduced in rats receiving CoPP. Liver fibrosis was evaluated 3 wk after BDL and the sirius red-positive area was found to be increased to about 7.8%. However, in CoPP pretreated rats sirius red- positive areas were increased to about 11.7% after BDL. Collagen-Ⅰα and TGF-β mRNA increased significantly by BDL. Again, this effect was increased by HO-1 overexpression.CONCLUSION: Hepatic fibrosis due to BDL is not reduced by the HO-1 inducer CoPP. In contrast, HO-1 overexpression increases liver injury in rats under conditions of experimental chronic cholestasis. | Matthias Froh Lars Conzelmann Peter Walbrun Susanne Netter Reiner Wiest Michael D Wheeler Mark Lehnert Takehiko Uesugi Jurgen Scholmerich Ronald G Thurman | 2007 | World Journal of Gastroenterology2007，13，25： | 4 |
| 20 | Role of HSP-90 for increased nNOS-mediated vasodilation in mesenteric arteries in portal hypertension显示文摘AIM:To explore the role of heat shock protein-90 (HSP-90) for nitrergic vasorelaxation in the splanchnic circulation in rats with and without portal hypertension. METHODS: Neuronal nitric oxide synthase (nNOS) and HSP-90 were analyzed by immunofluorescence, western blotting and co-immunoprecipitation in the mesenteric vasculature and isolated nerves of portal-vein-ligated (PVL) rats and sham operated rats. In vitro perfused de-endothelialized mesenteric arterial vasculature was preconstricted with norepinephrine (EC80) and tested for nNOS-mediated vasorelaxation by periarterial nerve stimulation (PNS, 2-12 Hz, 45V) before and after incubation with geldanamycin (specific inhibitor of HSP-90 signalling, 3 μg/mL) or L-NAME (non-specific NOSblocker, 10-4 mol/L). RESULTS: nNOS and HSP-90 expression was significantly increased in mesenteric nerves from PVL as compared to sham rats. Moreover, nNOS and HSP-90 were visualized in mesenteric nerves by immunofluorescence and immunoprecipitation of nNOS co-immunoprecitated HSP-90 in sham and PVL rats. PNS induced a frequencydependent vasorelaxation which was more pronounced in PVL as compared to sham rats. L-NAME and geldanamycin markedly reduced nNOS-mediated vasorelaxation abrogating differences between the study groups. The effect of L-NAME and geldanamycin on nNOS-mediated vasorelaxation was significantly greater in PVL than in sham animals. However, no difference in magnitude of effect between L-NAME and geldanamycin was noted. CONCLUSION: HSP-90 acts as a signalling mediator of nNOS-dependent nerve mediated vascular responses in mesenteric arteries, and the increased nitrergic vasorelaxation observed in portal hypertension is mediated largely by HSP-90. | Lukas Moleda Lars Jurzik Matthias Froh Erwin Gbele Claus Hellerbrand Rainer H Straub Jürgen Schlmerich Reiner Wiest | 2010 | World Journal of Gastroenterology2010，16，15： | 4 |
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