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5篇 您的检索式:作者名="Rainer Hoefgen"
    题名 作者 年代 出处 被引量
1General Regulatory Patterns of Plant Mineral Nutrient Depletion as Revealed by serat Quadruple Mutants Disturbed in Cysteine Synthesis显示文摘硫酸盐是为植物的必要 macronutrient。植物开发了策略应付硫酸盐缺乏,和另外的滋养的离子限制。然而,这些适应回答的规定和位于他们下面的协调信号仍然糟糕被描绘。O-acetylserine (美洲国家组织) 是硫酸盐饥饿的标记代谢物并且被推测了有发信号的功能。美洲国家组织被酶丝氨酸 acetyltransferase (SERAT ) 综合,它被五不同基因编码为 Arabidopsis。我们调查了保留了仅仅一功能的 isoform 的 SERAT 的四倍的猛烈异种。这些异种显示了硫酸盐饥饿的症状。而且,特别地,他们中的一些显示了典型地代表延长硫酸盐饥饿的显型与老朽或压力回答联系的发展节目。因此,我们从这些异种把代谢物和 transcriptome 数据与 N- , P- , K- ,和弄空 S 的植物作比较。这与一般 nutrient-depletion-induced 老朽(NuDIS ) 揭示了许多类似,显示为滋养饥饿的回答的存在规章的节目的招募。能涉及这些过程的几候选人基因被识别,包括抄写因素和另外的规章的蛋白质,以及他们的目标基因的功能的范畴。这些结果构画出在硫酸盐应力下面控制植物开发的规章的网络的部件,形成阐明的进一步的调查的一个基础完全的网络。接着,这将推进我们对另外的滋养的压力的一个范围的植物回答的更宽广的理解。Mutsumi Watanabe Hans-Michael Hubberten Kazuki Saito Rainer Hoefgen 2010Molecular Plant2010,3,2:6
2Impact of elevated H2S on metabolite levels, ac- tivity of enzymes and expression of genes involved in cysteine metabolism 显示文摘Anja Riemenschneider Victoria Nikiforova Rainer Hoefgen 2005Plant Physiology and Biochemistry2005,43,:1
3Cloning and characterization of a cDNA encoding a cobalamin-independent methionine synthase from potato (Solanum tuberosum L.)显示文摘Michaela Zeh Georg Leggewie Rainer Hoefgen Holger Hesse 2002Plant Molecular Biology2002,,3:1
4Enhanced cystathionine β-lyase activity in transgenic potato plants does not force metabolite flow towards methionine显示文摘Stefanie Maimann Rainer Hoefgen Holger Hesse 2001Planta2001,,2:1
5Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development显示文摘In developing apple fruit,metabolic compartmentation is poorly understood due to the lack of experimental data.Distinguishing subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the technique.The work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit,covering the major events occurring during fruit development(cell division,cell expansion,and maturation).Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments(cytosol,plastids,and vacuole).Evidence is presented that most of the sugars and organic acids were predominantly located in the vacuole,whereas some of the amino acids were distributed between the cytosol and the vacuole.The results showed a shift in the plastid marker from the lightest fractions in the early growth stage to the dense fractions in the later fruit growth stages.This implies that the accumulation of starch content with progressing fruit development substantially influenced the distribution of plastidial fragments within the non-aqueous density gradient applied.Results from this study provide substantial baseline information on assessing the subcellular compartmentation of metabolites in apple fruit in general and during fruit growth in particular.Wasiye F.Beshir Takayuki Tohge Mutsumi Watanabe Maarten L.A.TMHertog Rainer Hoefgen Alisdair R.Fernie Bart M.Nicolaï 2019Horticulture Research2019,6,1:0
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