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| 1 | Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays. | Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson | 2015 | World Journal of Gastroenterology2015，21，11： | 2 |
| 2 | A statistical analysis of conditional launch for gigabit ethernet links using multimode fiber显示文摘 | Webster M Raddatz L White I H et el | 1999 | J Lightwave Technol1999，17，9： | 1 |
| 3 | Overcoming the modal bandwidth limitation of multimode fiber by using pass-band modulation显示文摘 | RADDATZ L and WHITE I H | 1999 | IEEE Photonits Technology Letters1999，11，2： | 1 |
| 4 | Are external shocks responsible for the instability of output in low - income countries? 显示文摘 | Raddatz C | 2007 | Jour- nal of Development Economics2007，84，1： | 1 |
| 5 | PrimerArray:genome-scale primer design for DNA-microarray construction 显示文摘 | Dehio M Meyer TF | 2001 | Bioinformatics2001，17，1： | 1 |
| 6 | Liquidity needs and vulnerability to financial underdevelopment显示文摘 | Raddatz C | 2006 | Journal of Financial Economics2006，，80： | 1 |
| 7 | , 2006, 'Liquidity Needs and Vul- nerability to Financial Underdevelopment' 显示文摘 | Raddatz C | 2006 | Journal of Financial Economics2006，80，3： | 1 |
| 8 | Combined biogeophysical and biogeochemical effects of large-scale forest cover changes in the MPI earth system model显示文摘 | Bathiany S. Claussen M. Brovkin V. Raddatz T. Gayler V | 2010 | Biogeosciences2010，，68： | 1 |
| 9 | Coagulation activation markers do not correlate with the clinical risk of thrombosis in pregnant women显示文摘 | Bombelin T Raddatz Muller P Fehr J | 2001 | Am J Obstet Genecol2001，184，3： | 1 |
| 10 | How reliable is MRI in diagnosing cartilaginous lesions in patients with first and recurrent lateral patellar dislocations显示文摘 | Engelhardt LV Raddatz M Bouillon B | 2010 | BMC Musculoskelet Disord2010，11，： | 1 |
| 11 | Quantitative measurement of cytokine mRNA in inflammatory bowel disease:relation to clinical and endoscopic activity and outcome显示文摘 | Raddatz D Bockemuhl M Ramadori G | 2005 | Eur J Gastroenterol Hepatol2005，17，5： | 1 |
| 12 | In fluence of gender on stimulated cytokine response in patients of severe sepsis显示文摘 | Bauer I Bauer M Raddatz A | 2006 | Anaesthesist2006，55，5： | 1 |
| 13 | Liquidity Needs and Vulnerability to Financial Underdevelopment显示文摘 | RADDATZ C | 2006 | Journal of Financial Economics2006，80，： | 1 |
| 14 | Fasting hyperglucagone-mia in patients with transjugular intrahepatic portosystemic shunts(TIPS)显示文摘 | Raddatz D Rosbach C Buchwald A | 2005 | Exp Clin Endocrinol Diabetes2005，113，5： | 1 |
| 15 | Liquidity Needs and Vulnerability to Financial Underdevelopment显示文摘 | Raddatz C | 2006 | Journal of Financial Economics2006，，3： | 1 |
| 16 | Fasting hyperglucagonemia in patients with transjugu|ar intrahepatic portosystemic shunts (TIPS) 显示文摘 | Raddatz D Rosbach C Buchwald A | 2008 | Exp Clin Endocrinol Diabetes2008，116，8： | 1 |
| 17 | Modelling of R-curve behaviour in ceramic/metal composites 显示文摘 | RADDATZ O SCHNEIDER G A CLAUSSEN N | 1998 | Acta Materialia1998，46，18： | 1 |
| 18 | Predictors of Nonalcoholic Steatohepatitis (NASH) in Obese Patients Undergoing Gastric Bypass显示文摘 | Camilo Boza MD Arnoldo Riquelme MD Luis Iba?ez MD Ignacio Duarte MD Enrique Norero MD Paola Viviani MD Alejandro Soza MD Jose Ignacio Fernandez MD Alejandro Raddatz MD Sergio Guzman MD Marco Arrese MD | 2005 | Obesity Surgery2005，，8： | 1 |
| 19 | Glucocorticoid receptor expression in inflammatory bowel disease:evidence for a mucosal down-regulation in steroid-unresponsive ulcerative colitis显示文摘 | Raddatz D Middel P Bockemuhl M | 2004 | Aliment Pharmacol Ther2004，19，1： | 1 |
| 20 | Stable Sr-isotope, Sr/Ca, Mg/Ca, Li/Ca and Mg/Li ratios in the scleractinian cold-water coral Lophelia pertusa显示文摘 | RADDATZ J LIEBETRAU V ROGGEBERG A HATHORNE E KRABBENHOFT A EISENHAUER A BOttM F VOLLSTAEDT H FIETZKE J LOPEZ CORREA M FREIWALD A DULLO W | 2013 | Chemical Geology2013，352，： | 1 |
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