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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Polyamines reduces lipid peroxidation induced by different pro-oxidant agents显示文摘 | Nádia Aléssio Velloso Bellé Gerusa Duarte Dalmolin Graciela Fonini Maribel Antonello Rubin Jo?o Batista Teixeira Rocha | 2004 | Brain Research2004，，2： | 3 |
| 3 | Quality of life, work productivity impairment and healthcare resources in inflammatory bowel diseases in Brazil显示文摘BACKGROUND Inflammatory bowel diseases(IBD)have been associated with a low quality of life(QoL)and a negative impact on work productivity compared to the general population.Information about disease control,patient-reported outcomes(PROs),treatment patterns and use of healthcare resources is relevant to optimizing IBD management.AIM To describe QoL and work productivity and activity impairment(WPAI),treatment patterns and use of healthcare resources among IBD patients in Brazil.METHODS A multicenter cross-sectional study included adult outpatients who were previously diagnosed with moderate to severe Crohn’s disease(CD)or ulcerative colitis(UC).At enrolment,active CD and UC were defined as having a Harvey Bradshaw Index≥8 or a CD Activity Index≥220 or calprotectin>200μg/g or previous colonoscopy results suggestive of inadequate control(per investigator criteria)and a 9-point partial Mayo score≥5,respectively.The PRO assessment included the QoL questionnaires SF-36 and EQ-5D-5L,the Inflammatory Bowel Disease Questionnaire(IBDQ),and the WPAI questionnaire.Information about healthcare resources and treatment during the previous 3 years was collected from medical records.Chi-square,Fisher’s exact and Student’s t-/Mann-Whitney U tests were used to compare PROs,treatment patterns and the use of healthcare resources by disease activity(α=0.05).RESULTS Of the 407 patients in this study(CD/UC:64.9%/35.1%,mean age 42.9/45.9 years,54.2%/56.6%female,38.3%/37.1%employed),44.7%/25.2%presented moderate-to-severe CD/UC activity,respectively,at baseline.Expressed in median values for CD/UC,respectively,the SF-36 physical component was 46.6/44.7 and the mental component was 45.2/44.2,the EQ-visual analog scale score was 80.0/70.0,and the IBDQ overall score was 164.0/165.0.Moderate to severe activity,female gender,being unemployed,a lower educational level and lower income were associated with lower QoL(P<0.05).Median work productivity impairment was 20%and 5%for CD and UC patients,respectively,and activity impairment was 30%,the latter being higher among patients with moderate to severe disease activity compared to patients with mild or no disease activity(75.0%vs 10.0%,P<0.001).For CD/UC patients,respectively,25.4%/2.8%had at least one surgery,38.3%/19.6%were hospitalized,and 70.7%/77.6%changed IBD treatment at least once during the last 3 years.The most common treatments at baseline were biologics(75.3%)and immunosuppressants(70.9%)for CD patients and 5-ASA compounds(77.5%)for UC patients.CONCLUSION Moderate to severe IBD activity,especially among CD patients,is associated with a substantial impact on QoL,work productivity impairment and an increased number of IBD surgeries and hospitalizations in Brazil. | Rogerio Serafim Parra Julio MF Chebli Heda MBS Amarante Cristina Flores Jose ML Parente Odery Ramos Milene Fernandes Jose JR Rocha Marley R Feitosa Omar Feres Antonio S Scotton Rodrigo B Nones Murilo M Lima Cyrla Zaltman Carolina D Goncalves Isabella M Guimaraes Genoile O Santana Ligia Y Sassaki Rogerio S Hossne Mauro Bafutto Roberto LK Junior Mikaell AG Faria Sender J Miszputen Tarcia NF Gomes Wilson R Catapani Anderson A Faria Stella CS Souza Rosana F Caratin Juliana T Senra Maria LA Ferrari | 2019 | World Journal of Gastroenterology2019，25，38： | 2 |
| 4 | Nonalcoholic fatty liver disease in asymptomatic Brazilian adolescents显示文摘AIM:To evaluate the prevalence and clinical characteristics of Nonalcoholic fatty liver disease(NAFLD) among asymptomatic Brazilian adolescents. METHODS:Transversal observational study included asymptomatic adolescents with central obesity from private and public schools in Salvador-Bahia,northeastern Brazil.The children answered a questionnaire that included age,gender,race,and medical history,and were submitted to a complete physical exam and abdominal ultrasound.Biochemical exams included:ALT,AST,GGT,C reactive protein(CRP),fasting glucose,insulin,cholesterol and triglycerides.Criteria for NAFLD included:the presence of steatosis in ultrasound and/or high level of ALT,negative or occasional historic of intake of alcohol(≤140 g/wk),negative investigation for hepatitis A,B,C,auto-immune hepatitis,Wilson disease and hemochromatosis.RESULTS:From October,2005 to October,2006,the study included 1801 subjects between 11 and 18 years of age and a mean age of 13.7±2.0 years.One hundred ninety-nine had central obesity.The prevalence of NAFLD was 2.3%,most of whom were male and white.Insulin resistance(IR)was observed in 22.9% of them and had positive correlations with ALT and GGT(P<0.05).Elevated CRP was observed in 6.9% of the cases;however,it was not associated with WC,IR or liver enzymes. CONCLUSION:The prevalence of NAFLD in Brazilian adolescents was low.The ethnicity may have influence this frequency in the population studied,which had a large proportion of African descendents. | Raquel Rocha Helma Pinchemel Cotrim Almir Galvo Vieira Bitencourt Daniel Batista Valente Barbosa Adméia Souza Santos Alessandro de Moura Almeida Bruno Cunha Isabel Guimares | 2009 | World Journal of Gastroenterology2009，15，4： | 2 |
| 5 | Krebs Cycle Intermediates Modulate Thiobarbituric Acid Reactive Species (TBARS) Production in Rat Brain In Vitro显示文摘 | Robson L. Puntel Cristina W. Nogueira Jo?o B. T. Rocha | 2005 | Neurochemical Research2005，，2： | 2 |
| 6 | The effects of ebsclen on glutamate uptake by synaptic vesicles from rat brain 显示文摘 | PORCIUNCULA L O ROCHA J B GHISLENI G | 2004 | Brain Res2004，1027，12： | 1 |
| 7 | Effects of trichothecene mycotoxins on eukaryotic cells: A review显示文摘 | Rocha O Ansari K Doohan F M | 2005 | Food Additives and Contaminants2005，22，4： | 1 |
| 8 | Entrepreneurship and development: the role of cluster显示文摘 | ROCHA HECTOR O | 2004 | Small Business Economies2004，23，5： | 1 |
| 9 | Fluid and electrolyte balance during the first week of life and risk of bronchopulmonary dysplasia in the preterm neonate 显示文摘 | Rocha G Ribeiro O Guimaraes H | 2010 | Clinics (Sao Paulo)2010，65，7： | 1 |
| 10 | The use of PCR-RFLP to genetically distinguish the morphologically close species: Ceriodaphnia dubia Richard, 1894 and Ceriodaphnia silvestrii Daday, 1902 (Crustacea Cladocera)显示文摘 | ABREU M J SANTOS-WISNIEWSKI M J ROCHA O | 2010 | Brazilian Journal Biology2010，70，1： | 1 |
| 11 | Acute and Chronic Effects of Atrazine and Sodium Dodecyl Sulfate on the Tropical Freshwater Cladoceran Pseudosida ramosa 显示文摘 | FREITAS E C ROCHA O | 2012 | Ecotoxicology2012，21，5： | 1 |
| 12 | Oil sludge treatment by photocatalysis applying black and white light显示文摘 | ROCHA O R S DANTAS R F DUARTE M M M B | 2010 | Chemical Engineering Journal2010，157，1： | 1 |
| 13 | Isolation, identification, and activity in vitro of killer yeasts against Colletotrichum gloeosporioides isolated from tropical fruits显示文摘 | Jaqueline Rabelo de Lima Luciana Rocha Barros Gon?alves Luciana Rocha Brand?o Carlos Augusto Rosa Francisco Marto Pinto Viana | 2013 | J Basic Microbiol2013，，7： | 1 |
| 14 | Impacts of the emerald ash borer (EAB) eradication and tree mortality: potential for a secondary spread of invasive plant species显示文摘 | Hausman C E Jaeger J F Rocha O J | 2010 | Biological Invasions2010，12，7： | 1 |
| 15 | Microstructural Evolution at the Initial Stages of Continuous Annealing of Cold Rolled Dual phase Steels 显示文摘 | Rocha R O Melo T M F Pereloma E V | 2005 | Materials Science and Engineering2005，391，12： | 1 |
| 16 | Natural triterpenoids from Cecropia lyratiloba are cytotoxic to both sensitive and multidrug resistant leukemia cell lines显示文摘 | Rocha GG Sim(o)es M Lúcio KA | 2007 | Bioorg Med Chem2007，15，： | 1 |
| 17 | Epilithic di- atoms as indicators of water quality in the Gravata river, Rio Grandedo Sul,Brazil 显示文摘 | Salomoni S E Rocha O Callegaro V L | 2006 | Hydrobiologia2006，559，1： | 1 |
| 18 | Emission quantum yield of europium (Ⅲ) mixed complexes with thenoyhriiluoroacetonate and some aromatic ligands 显示文摘 | Silva F R G E Menezes J F S Rocha G B Rocha G B Alves S Brito H F Longo R L Malta O L | 2000 | Journal of Alloys and Compounds2000，303，304： | 1 |
| 19 | Entrepreneurship: The role of clusters theoretical perspectives and empirical evidence from Germany显示文摘 | Rocha H O Stemberg R | 2005 | Small Business Economics2005，24，3： | 1 |
| 20 | Synthesis of 1,8-cineole and 1,4-cineole by isomerization of α-terpineol catalyzed by heteropoly acid显示文摘 | LE?O LANA E J DA SILVA ROCHA K A KOZHEVNIKOV I V | 2006 | Journal of Molecular Catalysis A:Chemical2006，259，12： | 1 |
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