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| 1 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 2 | Association of diabetes with amnestic and nonamnestic mild cognitive impairment 显示文摘 | Roberts RO Knopman DS Geda YE | 2014 | Alzheimers Dement2014，10，： | 1 |
| 3 | Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons显示文摘 | Kim JH Roberts DS Hu Y | 2012 | Neurochem^2012，120，2： | 1 |
| 4 | Dosimetry of a w-188/Re-188 beta line source for endovascuar brachytherapy 显示文摘 | Hafeli UO Roberts WK Meier DS | 2000 | Med Phys2000，27，4： | 1 |
| 5 | Association of duration and severity of diabetes mellitus with mild cognitive inoairment 显示文摘 | ROBERTS RO GEDA YONASE KNOPMAN DS | 2008 | Arch Neurol2008，65，8： | 1 |
| 6 | Comparative molecular pharmacology in leukemia iliaio cells of the antheracene antheracene anticancer dnags mitoxantrone and bisantrene显示文摘 | Bowden GT Roberts R Allbert Ds | 1985 | Cancer Res1985，45，： | 1 |
| 7 | Physical exercise,aging,and mild cognitive impairment:a population-based study显示文摘 | Geda YE Roberts RO Knopman DS | 2010 | Arch Neurol2010，67，1： | 1 |
| 8 | Liquid chromatographic method for determination citreoviridin in corn and rice显示文摘 | Robert DS Judith IG Odette LS | 1988 | J Assoc of Anal Chem1988，71，4： | 1 |
| 9 | Genetic deletion of preg-nancy-associated plasma protein-A is associated with resist-ance to atheroselerotic lesion development in apolipoprotein E-deficient mice challenged with a high-fat diet显示文摘 | Sean CH Robert DS Conover CA | | 0，，12： | 1 |
| 10 | Higher risk of progres- sion to dementia in mild cognitive impairment cases who revert to nor- mal显示文摘 | Roberts RO Knopman DS Mielke MM | 2014 | Neurology2014，82，4： | 1 |
| 11 | Mild cognitive impairment:ten years later显示文摘 | Petersen RC Roberts RO Knopman DS | | 0，，12： | 1 |
| 12 | Effect of age on sensory gating of the sleep state-dependent P1/P50 midlatency auditory evoked potential显示文摘 | Lisa R Robert DS Edgar GR | 2000 | Sleep Research Online2000，3，3： | 1 |
| 13 | Association of C - reactive protein with mild cognitive impairment显示文摘 | Roberts RO Geda YE Knopman DS | 2009 | Alzheimers Dement2009，5，5： | 1 |
| 14 | Duration and severity of diabetes are associated with mild cognitive impairment 显示文摘 | Roberts RO Geda YE Knopman DS | 2008 | Arch Neurol2008，65，8： | 1 |
| 15 | Prevalence of mild cognitive impairment is higher in men:The Mayo Clinic Study of Aging显示文摘 | Petersen RC Roberts RO Knopman DS | | 0，，10： | 1 |
| 16 | Genetic deletion of Pregnancyassociated plasma protein-A is associated with resistance to atherosclerotic lesion development in apolipoprotein E - deficient mice challenged with a high-fat diet 显示文摘 | Sean CH Robert DS Conover CA | 2007 | Circ Res2007，100，12： | 1 |
| 17 | The incidence of MCI differs by subtype and is higher in men: the Mayo Clinic Study ofAging显示文摘 | Roberts RO Geda Y E Knopman DS | 2012 | Neurology2012，78，5： | 1 |
| 18 | Mild cognitive impairment:ten years later显示文摘 | Petersen RC Roberts RO Knopman DS | 2009 | Arch Neurol2009，66，12： | 1 |
| 19 | Physical exercise, aging, and mild cognitive impairment: a population-based study 显示文摘 | Geda YE Roberts RO Knopman DS | 2010 | Arch Neurol2010，67，1： | 1 |
| 20 | Association of C-reac- tive protein with mild cognitive impairment 显示文摘 | Roberts RO Geda YE Knopman DS | 2009 | Alzheimers De- ment2009，5，： | 1 |
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