|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Incretins and selective renal sodium-glucose co-transporter 2 inhibitors in hypertension and coronary heart disease显示文摘Hyperglycemia is associated with an increased risk of cardiovascular disease,and the consequences ofintensive therapy may depend on the mechanism of the anti-diabetic agent(s)used to achieve a tight control.In animal models,stable analogues of glucagon-like peptide-1(GLP-1)were able to reduce body weight and blood pressure and also had favorable effects on ischemia following coronary reperfusion.In a similar way,dipeptidyl peptidase IV(DPPIV)showed to have favorable effects in animal models of ischemia/reperfusion.This could be due to the fact that DPPIV inhibitors were able to prevent the breakdown of GLP-1 and glucose-dependent insulinotropic polypeptide,but they also decreased the degradation of several vasoactive peptides.Preclinical data for GLP-1,its derivatives and inhibitors of the DPPIV enzyme degradation suggests that these agents may be able to,besides controlling glycaemia,induce cardio-protective and vasodilator effects.Notwithstanding the many favorable cardiovascular effects of GLP-1/incretins reported in different studies,many questions remain unanswered due the limited number of studies in human beings that aim to examine the effects of GLP-1 on cardiovascular endpoints.For this reason,long-term trials searching for positive cardiovascular effects are now in process,such as the CAROLINA and CARMELINA trials,which are supported by small pilot studies performed in humans(and many more animal studies)with incretin-based therapies.On the other hand,selective renal sodium-glucose co-transporter 2 inhibitors were also evaluated in the prevention of cardiovascular outcomes in type 2 diabetes.However,it is quite early to draw conclusions,since data on cardiovascular outcomes and cardiovascular death are limited and long-term studies are still ongoing.In this review,we will analyze the mechanisms underlying the cardiovascular effects of incretins and,at the same time,we will present a critical position about the real value of these compounds in the cardiovascular system and its protection. | Ramiro A Sanchez Hugo Sanabria Cecilia de los Santos Agustin J Ramirez | 2015 | World Journal of Diabetes2015，6，11： | 5 |
| 3 | Fecal immunochemical test accuracy in average-risk colorectal cancer screening显示文摘AIM:To assess the fecal immunochemical test(FIT)accuracy for colorectal cancer(CRC)and advanced neoplasia(AN)detection in CRC screening.METHODS:We performed a multicentric,prospective,double blind study of diagnostic tests on asymptomatic average-risk individuals submitted to screening colonoscopy.Two stool samples were collected and the fecal hemoglobin concentration was determined in the first sample(FIT1)and the highest level of both samples(FITmax)using the OC-sensor.Areas under the curve(AUC)for CRC and AN were calculated.The best FIT1and FITmax cut-off values for CRC were determined.At this threshold,number needed to scope(NNS)to detect a CRC and an AN and the cost per lesion detected were calculated.RESULTS:About 779 individuals were included.An AN was found in 97(12.5%)individuals:a CRC in 5(0.6%)and an advanced adenoma(≥10 mm,villous histology or high grade dysplasia)in 92(11.9%)subjects.For CRC diagnosis,FIT1 AUC was 0.96(95%CI:0.95-0.98)and FITmax AUC was 0.95(95%CI:0.93-0.97).For AN,FIT1 and FITmax AUC were similar(0.72,95%CI:0.66-0.78 vs 0.73,95%CI:0.68-0.79,respectively,P=0.34).Depending on the number of determinations and the positivity threshold cut-off used sensitivity for AN detection ranged between 28%and 42%and specificity between 91%and 97%.At the best cut-off point for CRC detection(115 ng/mL),the NNS to detect a CRC were 10.2 and 15.8;and the cost per CRC was 1814€and 2985€on FIT1 and FITmax strategies respectively.At this threshold the sensitivity,NNS and cost per AN detected were 30%,1.76,and 306€,in FIT1 strategy,and 36%,2.26€and 426€,in FITmax strategy,respectively.CONCLUSION:Performing two tests does not improve diagnostic accuracy,but increases cost and NNS to detect a lesion. | Vicent Hernandez Joaquin Cubiella M Carmen Gonzalez-Mao Felipe Iglesias Concepción Rivera M Begoa Iglesias Lucía Cid Ines Castro Luisa de Castro Pablo Vega Jose Antonio Hermo Ramiro Macenlle Alfonso Martínez-Turnes David Martínez-Ares Pamela Estevez Estela Cid M Carmen Vidal Angeles López-Martínez Elisabeth Hijona Marta Herreros-Villanueva Luis Bujanda Jose Ignacio Rodriguez-Prada the COLONPREV study investigators | 2014 | World Journal of Gastroenterology2014，20，4： | 4 |
| 4 | Efficient metallization of magnetite concentrate by reduction with agave bagasse as a source of reducing agents显示文摘The reduction behavior and metallization degree of magnetite concentrate with agave bagasse were investigated in an inert atmosphere.The effects of temperature,biomass content,and residence time on reduction experiments and metallization degree were investigated by X-ray diffraction and scanning electron microscopy.Compared with other types of biomass,agave bagasse had lower contents of nitrogen,sulfur,and ash.X-ray diffraction analysis showed that the metallization degree improved with increasing temperature and biomass content.Complete metallization was achieved at 1100℃ for 30 min with 65:35 and 50:50 ratios of the magnetite concentrate to the agave bagasse.These results demonstrate that agave bagasse promotes the efficient metallization of magnetite concentrate without the external addition of a reducing agent.Therefore,this biomass is a technical suitable alternative to replace fossil fuels in steelmaking. | Diana Cholico-González NoemíOrtiz Lara Mario Alberto Sánchez Miranda Ricardo Morales Estrella Ramiro Escudero García Carlos ALeón Patiño | 2021 | International Journal of Minerals,Metallurgy and Materials2021，28，4： | 2 |
| 5 | Combustion optimisation of biomass residue pellets for domestic heating with a mural boiler显示文摘 | GONZALEZ J F MGONZALEZ-GARCIA C RAMIRO A | 2004 | Biomass and Bioenergy2004，27，： | 1 |
| 6 | MicroRNA control of lymphocyte differentiation and function显示文摘 | Belver L Papavasiliou F N Ramiro A R | 2011 | Curr Opin Immunol2011，23，3： | 1 |
| 7 | Higher disease activity leads to more structural damage in the spine in ankylosing spondylitis: 12-year longitudinal data from the OASIS cohort显示文摘 | RAMIRO S VAN DER HEIJDE D VAN TUBERGEN A | 2014 | Annals of the Rheumatic Diseases2014，73，8： | 1 |
| 8 | Transcatheter aortic valve implantation in very elderly patients: immediate results and medium term follow-up显示文摘ObjectiveTo 评估立即的 transcatheter 在有严重、征兆的大动脉的狭窄的很老的病人的大动脉的阀门培植(TAVI ) 结果和中期的后续(作为).MethodsThis multicenter,观察、未来的学习在三所医院里被执行。我们包括了连续很老(>85 年) 病人与作为严重由 TAVI 对待。主要端点是在包括的二 years.ResultsThe 研究从任何原因评估死亡率有一个平均数的 160 个连续病人 87 ± 变老;2.1 年(从 85 ~ 94 年的范围) 和 18.8%± 的吝啬的逻辑 EuroSCORE;11.2% 与 57 (35.6%) 病人得分 ≥20% 。程序的成功率是 97.5% ,与 25 (15.6%) 与主要流血经历尖锐复杂并发症的病人(最经常) 。全球死亡率在住院期间是 8.8%(n = 14 ) 并且 30 天的死亡率是 10%(n = 16 ) 。中部在时期上面列在后面是 252.24 ±232.17 天。在后续时期期间, 28 (17.5%) 病人们死了(他们中的 17 个由于心脏的原因) 。估计的二年全面、心脏的幸存率使用 Kaplan-Meier 方法分别地是 71% 和 86.4% 。比例的危险回归给看了的考克斯可变 EuroSCORE ≥20 是与全面 mortality.ConclusionsTAVI 联系的唯一的变量在很老的病人的一张选择人口安全、有效。我们的调查结果支持在病人的这个复杂的组的这个新过程的采纳。 | Isaac Pascual Antonio J Mu oz-García Diego López-Otero Pablo Avanzas Manuel F Jimenez-Navarro Belén Cid-Alvarez Raquel del Valle Juan H Alonso-Briales Raimundo Ocaranza-Sanchez José M Hemández Ramiro Trillo-Nouche César Morís | 2015 | Journal of Geriatric Cardiology2015，12，4： | 1 |
| 9 | Local search and genetic algorithm for the job shop scheduling problem with sequence dependent setup times显示文摘 | CAMINO R RAMIRO V MIGUEL A | 2010 | Journal of Heuristics2010，16，2： | 1 |
| 10 | Evolution of radiographic damage in ankylosing spon- dylitis: a 12 year prospective follow-up of the OASIS study显示文摘 | RAMIRO S STOLWIJK C VAN TUBERGEN A | 2013 | Annals of the Rheumatic Diseases2013，，： | 1 |
| 11 | Pyrolysis gasification of agricultural residues by carbon dioxide in the presence of different additives:influence of variables显示文摘 | Encinar J M Beltran F J Ramiro A | 1998 | Fuel Processing Technology1998，55，3： | 1 |
| 12 | Use of energy crops for domestic heating with a mural boiler 显示文摘 | Juan F GonzOlez Carmen M GonzOlez-Garcta Antonio Ramiro et a/ | 2006 | Fuel Processing Technology2006，87，8： | 1 |
| 13 | Local search and genetic algorithm for the job shop scheduling problem with sequence dependent setup times 显示文摘 | CAMINO R RAMIRO V MIGUEL A | 2010 | Journal of Heuristics2010，16，2： | 1 |
| 14 | Pyrolysis-gasification of agricultural residues by carbon dioxide in the presence of different additives: influence of variables显示文摘 | ENCINAR J M BELTRAN F J RAMIRO A | 1998 | Fuel Processing Technology1998，55，： | 1 |
| 15 | Relativistic electronic structure of an icosahedral Au12Pd cluster显示文摘 | Ramiro A P Lucia H A | 1999 | Chem Phys Lett1999，303，： | 1 |
| 16 | An easy synthesis of pyranopyrimidines显示文摘 | Herrera A Martinez - Alvarez R Ramiro P | 2006 | Monatshefte fur Chemie2006，137，： | 1 |
| 17 | Evolution of radiographic damage in ankylosing spondylitis: a 12 year prospective follow-up of the OASIS study 显示文摘 | Ramiro S Stolwijk C van Tubergen A | 2015 | Ann Rheum Dis2015，74，1： | 1 |
| 18 | Pyrolysis of two agricultural residues:Olive and grape bagasse:Influence of particle size and temperature显示文摘 | ENCINAR J M BELTRAN F J BERNALTE A RAMIRO A GONZALEZ J F | 1996 | Biomass Bioenergy1996，11，5： | 1 |
| 19 | Development of new syndesmophytes and bridges in ankylosing spondylitis and their predictors : a longitudinal study显示文摘 | van Tubergen A Ramiro S van der Heijde D | 2012 | Ann Rheum Dis2012，71，4： | 1 |
| 20 | Uveal melanoma expresses NK-1 receptors and cyclos- porin A induces apoptosis in human melanoma cell lines over- expressing the NK-1 receptor显示文摘 | Gonzcilez Ortega A Stlnchez Vaderrdbanos E Ramiro Fuentes S | 2014 | Peptides2014，55，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员