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| 1 | High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes显示文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening(HTS) data for 3 762 distinct global gene knockout(KO) mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type(WT) cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging,ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass: 23 previously reported genes(Calcr, Cebpb, Crtap, Dcstamp, Dkk1, Duoxa2, Enpp1, Fgf23, Kiss1/Kiss1 r, Kl(Klotho),Lrp5, Mstn, Neo1, Npr2, Ostm1, Postn, Sfrp4, Slc30a5, Slc39a13, Sost, Sumf1, Src, Wnt10b), five novel genes extensively characterized(Cldn18, Fam20 c, Lrrk1, Sgpl1, Wnt16), five novel genes with preliminary characterization(Agpat2, Rassf5, Slc10a7, Slc26a7, Slc30a10) and three novel undisclosed genes coding for potential osteoporosis drug targets. | Robert Brommage Jeff Liu Gwenn M Hansen Laura L Kirkpatrick David G Potter Arthur T Ss Brian Zambrowicz David R Powell Peter Vogel | 2014 | Bone Research2014，2，3： | 7 |
| 2 | Morphological and functional evaluation of chronic pancreatitis with magnetic resonance imaging显示文摘Magnetic resonance imaging(MRI)techniques for assessment of morphology and function of the pancreas have been improved dramatically the recent years and MRI is very often used in diagnosing and follow-up of chronic pancreatitis(CP)patients.Standard MRI including fat-suppressed T1-weighted and T2-weighted imaging techniques reveal decreased signal and glandular atrophy of the pancreas in CP.In contrast-enhanced MRI of the pancreas in CP the pancreatic signal is usually reduced and delayed due to decreased perfusion as a result of chronic inflammation and fibrosis.Thus,morphological changes of the ductal system can be assessed by magnetic resonance cholangiopancreatography(MRCP).Furthermore,secretin-stimulated MRCP is a valuable technique to evaluate side branch pathology and the exocrine function of the pancreas and diffusion weighted imaging can be used to quantify both parenchymal fibrotic changes and the exocrine function of the pancreas.These standard and advanced MRI techniques are supplementary techniques to reveal morphological and functional changes of the pancreas in CP.Recently,spectroscopy has been used for assessment of metabolite concentrations in-vivo in different tissues and may have the potential to offer better tissue characterization of the pancreas.Hence,the purpose of the present review is to provide an update on standard and advanced MRI techniques of the pancreas in CP. | Tine Maria Hansen Matias Nilsson Mikkel Gram Jens Brφndum Frφkjr | 2013 | World Journal of Gastroenterology2013，19，42： | 7 |
| 3 | Oxygen radical formation does not have an impact in the treatment of severe acute experimental pancreatitis using free cellular hemoglobin显示文摘瞄准:Microcirculatory 机能障碍和免费的氧激进分子是在严重尖锐胰腺炎的致病的重要因素。另外的氧交货可能每氧化提高类脂化合物,但是可以也改进胰腺的微循环。这研究在严重尖锐胰腺炎的一个啮齿类动物模型在氧激进分子和微循环的形成上估计免费细胞的牛的血红素的效果。方法:在尖锐胰腺炎 Wistar 老鼠的正式就职以后的十五分钟收到了任何一个 0.8 mL 牛的血红素(HBOC-200 ) , hydroxyethyl 淀粉(HES ) 或保证血量正常替换的生理盐水的 2.4 mL。在检查的 6 h 以后,胰每氧化产品 malondialdehyde (MDA ) 为类脂化合物的间接测量被切除并且很快处理了并且在胰腺的织物减少了谷胱甘肽(GSH ) 。结果:HBOC-200 的单个申请改进了胰腺的微循环并且显著地减少了组织病理学说的织物损坏。MDA 的织物集中没在这些组之间不同。另外,在 GSH 层次的差别都没被检测。结论:不过 HBOC-200 和 HES 的单个申请改进胰腺的微循环,在类脂化合物的没有差别每氧化,产品被检测。另外的氧供应(HBOC-200 ) 的有益的效果不每氧化导致提高的类脂化合物。 | Helge Kleinhans Oliver Mann Paulus G Schurr Jussuf T Kaifi Bente Hansen Jakob R Izbicki Tim Strate | 2006 | World Journal of Gastroenterology2006，12，18： | 6 |
| 4 | Towards a standard diet-induced and biopsy-confirmed mouse model of non-alcoholic steatohepatitis: Impact of dietary fat source显示文摘BACKGROUND The trans-fat containing AMLN(amylin liver non-alcoholic steatohepatitis,NASH)diet has been extensively validated in C57BL/6J mice with or without the Lep^ob/Lep^ob(ob/ob)mutation in the leptin gene for reliably inducing metabolic and liver histopathological changes recapitulating hallmarks of NASH.Due to a recent ban on trans-fats as food additive,there is a marked need for developing a new diet capable of promoting a compatible level of disease in ob/ob and C57BL/6J mice.AIM To develop a biopsy-confirmed mouse model of NASH based on an obesogenic diet with trans-fat substituted by saturated fat.METHODS Male ob/ob mice were fed AMLN diet or a modified AMLN diet with trans-fat(Primex shortening)substituted by equivalent amounts of palm oil[Gubra amylin NASH,(GAN)diet]for 8,12 and 16 wk.C57BL/6J mice were fed the same diets for 28 wk.AMLN and GAN diets had similar caloric content(40%fat kcal),fructose(22%)and cholesterol(2%)level.RESULTS The GAN diet was more obesogenic compared to the AMLN diet and impaired glucose tolerance.Biopsy-confirmed steatosis,lobular inflammation,hepatocyte ballooning,fibrotic liver lesions and hepatic transcriptome changes were similar in ob/ob mice fed the GAN or AMLN diet.C57BL/6J mice developed a mild to moderate fibrotic NASH phenotype when fed the same diets.CONCLUSION Substitution of Primex with palm oil promotes a similar phenotype of biopsyconfirmed NASH in ob/ob and C57BL/6J mice,making GAN diet-induced obese mouse models suitable for characterizing novel NASH treatments. | Michelle L Boland Denise Oro Kirstine S T■lb■l Sebastian T Thrane Jens Christian Nielsen Taylor S Cohen David E Tabor Fiona Fernandes Andrey Tovchigrechko Sanne S Veidal Paul Warrener Bret R Sellman Jacob Jelsing Michael Feigh Niels Vrang James L Trevaskis Henrik H Hansen | 2019 | World Journal of Gastroenterology2019，25，33： | 3 |
| 5 | Immunoreactivity of CD45,a protein phosphotyrosine phosphatase,in Alzheimer' s disease 显示文摘 | Masliah E Mallory M Hansen L Alford M Albright T Terry R | 1991 | Acta Neuropathol( Berl )1991，83，1： | 2 |
| 6 | High expression of microRNA-625-3p is associated with poor response to first-line oxaliplatin based treatment of metastatic colorectal cancer显示文摘 | Mads H. Rasmussen Niels F. Jensen Line S. Tarpgaard Camilla Qvortrup Maria U. R?mer Jan Stenvang Tine P. Hansen Lise L. Christensen Jan Lindebjerg Flemming Hansen Benny V. Jensen Torben F. Hansen Per Pfeiffer Nils Brünner Torben F. ?rntoft Claus L. Anders | 2013 | Molecular Oncology2013，，3： | 2 |
| 7 | Global discrimination of land cover types from metrics derived from AVHRR pathfinder data 显示文摘 | DEFRIES R HANSEN M TOWNSHEND J | 1995 | Remote Sensing Environment1995，54，： | 2 |
| 8 | 西双版纳傣族及上海汉族群体HLA-DR2相关的DR/DQ单倍型分析显示文摘对44名西双版纳傣族和9名上海地区汉族DK2阳性个体进行了与其相关的DR/DQ单倍型组合的分析。傣族群体中DRBI-DR2亚型分布以*1602与*1502为最常见,其等位基因频率分别为43.6%与,40.0%和汉族群体中以*1501为主明显不同。傣族群体中共检出10种与DR2相关联的DR/DQ单倍型;最常见的是DRB1*1602、DRB5*0101、DQA1*0102、DQB1*0502(34.5%)与汉族及其他群体明显不同,本研究表明傣族不仅具有高频率的DR2,而且与DR2相关联的DRB1、DRB5、DQA1、DQB1单倍型组合有其独特性。 | 范丽安 杨珏琴 姚芳娟 董建中 许玲娣 陈仁彪 M Sirkong R Vongrathna P Longta S Lekmak D Chandanayingyong A Smith J Hansen | 1995 | 中华医学遗传学杂志1995，12，3： | 2 |
| 9 | Interferon-γ inhibits ghrelin expression and secretion via a somatostatin-mediated mechanism显示文摘AIM:To investigate if and how the proinflammatory cytokine interferon γ(IFNγ) affects ghrelin expression in mice.METHODS:The plasma concentration of ghrelin,andgastric ghrelin and somatostatin expression,were examined in wild-type mice and mice infected with Helicobacter pylori(H.pylori).Furthermore,ghrelin expression was examined in two achlorhydric mouse models with varying degrees of gastritis due to bacterial overgrowth.To study the effect of IFNγ alone,mice were given a subcutaneous infusion of IFNγ for 7 d.Finally,the influence of IFNγ and somatostatin on the ghrelin promoter was characterized.RESULTS:H.pylori infection was associated with a 50% reduction in ghrelin expression and plasma concentration.Suppression of ghrelin expression was inversely correlated with gastric inflammation in achlorhdyric mouse models.Subcutaneous infusion of IFNγ suppressed fundic ghrelin mRNA expression and plasma ghrelin concentrations.Finally,we showed that the ghrelin promoter operates under the control of somatostatin but not under that of IFNγ.CONCLUSION:Gastric infection and inflammation is associated with increased IFNγ expression and reduced ghrelin expression.IFNγ does not directly control ghrelin expression but inhibits it indirectly via somatostatin. | Jesper AB Strickertsson Kristina BV DΦssing Anna JM Aabakke Hans-Olof Nilsson Thomas VO Hansen Ulrich Knigge Andreas Kjr Torkel Wadstrm Lennart Friis-Hansen | 2011 | World Journal of Gastroenterology2011，17，26： | 2 |
| 10 | Improvement in Mental Health over Time in Northern Norway: A Prospective Study of a General Population Followed for 9 Years, with Special Emphasis on the Influence of Darkness in Winter显示文摘 | Nilsen LS Hansen V Olstad R | 2004 | Social Psychiatry and Psychiatric Epidemiology2004，，4： | 1 |
| 11 | Yeast beta-glucan amplifies phagocyte killing of iC3b-opsonized tumor cells via complement receptor 3-Syk-phosphatidylinositol 3-kinase pathway显示文摘 | Li B Allendorf D J Hansen R | 2006 | J Immunol2006，177，3： | 1 |
| 12 | Isolation and characterizationof the promoter of the human 5' - nucleotidase (CD73)2encoding gene显示文摘 | Hansen KR Resta R Webb CF | 1995 | Gene1995，167，12： | 1 |
| 13 | Indentation fracture response and damage resistance of Al2O3-ZrO2 compositesstrengthened by transformation-induced residual stresses显示文摘 | Hansen J J Cutler R A Shetty D K | 1988 | Journal of the American Ceramic Society1988，171，12： | 1 |
| 14 | CO2-Reforming of Methane over Transition Metals显示文摘 | Rostrup-Nielsen J R Bak Hansen J-H | 1993 | J Catal1993，144，： | 1 |
| 15 | An analytical expression for the gravity field of a polyhedral body with linealy varying density显示文摘 | HANSEN R O | 1999 | Geophysics1999，64，1： | 1 |
| 16 | De Novo Biosynthesis of Vanillin in Fission Yeast (Schizosaccharomyces i?ombe) and Bakers Yeast (Saccharomyces cerevisiae)显示文摘 | Hansen E H Moiler B L Koek G R | 2009 | Applied md Environmentalmicrobiology2009，75，9： | 1 |
| 17 | Consistent ranking ofvolafilitymo dels显示文摘 | Hansen P R Lunde A | 2006 | Journal of Econometrics2006，，6： | 1 |
| 18 | New steel framing system promises major savings in high-rise apartment显示文摘 | Hansen R J Leuessurier W J Pahl P J Pel-letier R J | 1966 | Architecture Record1966，139，6： | 1 |
| 19 | Early on-treatment prediction of response to peginterferon alfa-2a for HBeAg-negative chronic hepatitisB using HBsAg and HBV DNA levels 显示文摘 | Rijckborst V Hansen BE Cakaloglu Y Ferenci P Tabak F Akdogan M Simon K Akarca US Flisiak R Verhey E Van Vuuren AJ Boucher CA ter Borg MJ Janssen HL | 2010 | Hepatology2010，52，2： | 1 |
| 20 | Designing Realised Kernels to Measure the Ex-Post Variation of Equity Prices in the Presence of Noise显示文摘 | Barndorff-Nielsen O E Hansen P R Lunde A Shephard N | 2008 | Econometrica2008，76，： | 1 |
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