| 1 | Functional analysis of alternative splicing of the FLOWERING LOCUS T orthologous gene in Chrysanthemum morifolium显示文摘As the junction of floral development pathways,the FLOWERING LOCUS T(FT)protein called‘florigen’plays an important role in the process of plant flowering through signal integration.We isolated four transcripts encoding different isoforms of a FT orthologous gene CmFTL1,from Chrysanthemum morifolium cultivar‘Jimba’.Sequence alignments suggested that the four transcripts are related to the intron 1.Expression analysis showed that four alternative splicing(AS)forms of CmFTL1 varied depending on the developmental stage of the flower.The functional complement experiment using an Arabidopsis mutant ft-10 revealed that the archetypal and AS forms of CmFTL1 had the function of complementing late flower phenotype in different levels.In addition,transgenic confirmation at transcript level showed CmFTL1 and CmFTL1ast coexist in the same tissue type at the same developmental stage,indicating a post-transcriptional modification of CmFTL1 in Arabidopsis.Moreover,ectopic expression of different AS forms in chrysanthemum resulted in the development of multiple altered phenotypes,varying degrees of early flowering.We found that an alternative splicing form(CmFTL1-astE134)without the exon 2 lacked the ability causing the earlier flower phenotype.The evidence in this study indicates that complex alternative processing of CmFTL1 transcripts in C.morifolium may be associated with flowering regulation and hold some potential for biotechnical engineering to create early-flowering phenotypes in ornamental cultivars. | Yachao Mao Jing Sun Peipei Cao Rong Zhang Qike Fu Sumei Chen Fadi Chen Jiafu Jiang | 2016 | Horticulture Research2016，3，1： | 7 |
| 2 | Selective and stable Au-Cu bimetallic catalyst for CO-PROX显示文摘Gold-based catalysts are promising in CO preferential oxidation(CO-PROX)reaction in H_(2)-rich stream on account of their high intrinsic activity for CO elimination even at ambient temperature.However,the decrease of CO conversion at elevated temperature due to the competition of H_(2)oxidation,together with the low stability of gold nanoparticles,has posed a dear challenge.Herein,we report that Au-Cu bimetallic catalyst prepared by galvanic replacement method shows a wide temperature window for CO total conversion(30-100℃)and very good catalyst stability without deactivation in a 200-h test.Detailed characterizations combined with density functional theory(DFT)calculation reveal that the synergistic effect of Au-Cu,the electron transfer from Au to Cu,leads to not only strengthened chemisorption of CO but also weakened dissociation of H_(2),both of which are helpful in inhibiting the competition of H_(2)oxidation thus widening the temperature window for CO total conversion. | Feng Hong Guanjian Cheng Weihao Hu Shengyang Wang Qike Jiang Junhong Fu Botao Qiao Jiahui Huang | 2023 | Nano Research2023，16，7： | 0 |
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