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| 1 | Diagnostic value of glypican-3 in serum and liver for primary hepatocellular carcinoma显示文摘AIM:To evaluate the diagnostic value of glypican-3(GPC3) in serum and liver for primary hepatocellular carcinoma(HCC).METHODS:Serum levels of GPC3 and α-fetoprotein(AFP) were measured in 75 patients with primary HCC and 32 patients with liver cirrhosis.Expression of GPC3 and AFP in 58 HCC and 12 cirrhotic specimens was detected with immunohistochemical staining.RESULTS:When the cut-off value of serum GPC3 was set at 300 ng/L,its sensitivity and specificity for HCC were 47.0% and 93.5%,respectively.Among the 14 patients with HCC at stage according to the Barcelona Clinic Liver Cancer staging system,the serum GPC3 level was higher than 300 ng/L in 50%(7/14) patients,the serum AFP level was not ≥ 400 μg/L in any patient.Combined serum AFP and GPC3 significantly increased the sensitivity to the diagnosis of HCC.The GPC3 expression was detected in cytoplasm of HCC cells but not in hepatocytes and bile ducts of benign tumors.Among the 58 HCC patients,the GPC3 was expressed in 100%(28/28) patients with their serum AFP level ≥ 400 μg/L,and in 90%(27/30) patients with their AFP level < 400 μg/L,respectively.The GPC3 was weakly or negatively expressed in all paracarcinomatous and cirrhotic tissue samples.AFP positive HCC cells were only found in 1 out of the 58 HCC patients.CONCLUSION:GPC3 protein is a sensitive and specific serum marker for diagnosis of early HCC.Its expression in liver tissues can be used to discriminate tumor cells from benign hepatic cells. | Hui Liu Li-Jie Zhang Peng Li Yun Zhai Yu-Fen Tan Ning Li Hui-Guo Ding Chun-Feng Qu | 2010 | World Journal of Gastroenterology2010，16，35： | 57 |
| 2 | Effect of in-hospital medical complications on case fatality post-acute ischemic stroke: data from the China National Stroke Registry显示文摘 | WANG Peng-lian ZHAO Xing-quan YANG Zhong-hua WANG An-xin WANG Chun-xue LIU Li-ping WANGYi-long WANG Xin-gao JU Yi CHEN Sheng-yun CHEN Qi-dong QU Hui LU Jing-jing ZHANG Jing MA Rui-hua ZHANG Yu-mei WANG Yong-jun | 2012 | Chinese Medical Journal2012，，14： | 37 |
| 3 | 电针和经皮穴位电刺激技术在生殖医学中的应用:专家共识(英文)显示文摘本文从传统针刺治疗不孕症的选穴依据与现代电针/经皮穴位电刺激技术的相关原理着手,从最优刺激参数选择、穴位辨证、疗程与治疗次数的确定及基本原理等方面阐述了电针/经皮穴位电刺激技术在多囊卵巢综合症、取卵镇痛、卵巢储备功能降低、胚胎移植以及男性少/弱精症等生殖领域中的具体应用。首次对电针/经皮穴位电刺激技术在生殖领域优势病种中的应用进行了系统的分析和总结,在最优刺激参数选择、穴位辨证、疗程与治疗次数的确定等方面提出了一系列专家共识,并为临床实践提供了指导意见和理论依据。 | Fan QU Rong LI Wei SUN Ge LIN Rong ZHANG Jing YANG Li TIAN Guo-gang XING Hui JIANG Fei GONG Xiao-yan LIANG Yan MENG Jia-yin LIU Li-ying ZHOU Shu-yu WANG Yan WU Yi-jing HE Jia-yu YE Song-ping HAN Ji-sheng HAN | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 24 |
| 4 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 5 | A novel method for purification of recombinant adeno-associated virus vectors on a large scale显示文摘A novel method for recombinant adeno-associ-ated virus (rAAV) purification on large scale is described. The method involves three steps, including chloroform treatment, PEG/NaCl precipitation and chloroform extraction. The whole procedure can be performed in four hours. Using this purification method, we can reproducibly obtain, from 4 × 109 of proviral cells cultured in roller bottles, purified rAAV-GFP stocks with titers of around 5×1013 particles/mL and purity greater than 95%. The infectious titers of the vector stocks were up to 2×1012 TU/mL, thus particle-to-infectivity rate was about 25. Under an electronic microscope, most rAAV particles appeared full and a few were in intermediate form. Empty particles were rarely seen. The purified rAAV-GFP stocks have been successfully used in in vitro and in vivo transfection experiments. Therefore, this new method offers a simple, rapid and cost-effective way for large-scale rAAV purification. | WU Xiaobing DONG Xiaoyan WU Zhijian CAO Hui NIU Dongbin QU Jianguo WANG Hong HOU Yunde | 2001 | Chinese Science Bulletin2001，46，6： | 21 |
| 6 | The Role of Fibrocyte in the Pathogenesis of Silicosis显示文摘Exposure to free silica induces silicosis and myofibroblasts are regarded as primary effector cells.Fibrocytes can differentiate into myofibroblast.Therefore,the present study was designed to investigate whether | LI Juan YAO Wu HOU Jian Yong ZHANG Lin BAO Lei CHEN Hui Ting WANG Di YUE Zhong Zheng LI Yi Ping ZHANG Miao YU Xing Hao ZHANG Jian Hui QU Ya Qian HAO Chang Fu | 2018 | Biomedical and Environmental Sciences2018，31，4： | 20 |
| 7 | Solitary pulmonary nodules: comparison of multi-slice computed tomography perfusion study with vascular endothelial growth factor and microvessel density显示文摘背景独居的肺的小瘤(SPN ) 是胸拍上的最普通的调查结果之一。临床的实践的目的是以最少的侵略方法把恶意的小瘤区分开来与良性的小瘤并且做特定的诊断。这研究被瞄准用多片在 SPN 评估在灌注成像特征和 microvessel 密度和脉管的 endothelial 生长因素(VEGF )(MVD ) 之间的关联计算断层摄影术(MSCT ) ;并且流动模式和血流动为 SPN 血提供理论基础量的 features.Also ,在良性、恶意的 SPNs.Methods 的微分诊断为方法的临床的申请价值的讨论叫的学习有 SPN 的 68 个病人经历了动态对比提高了的多地点( nonionic 对比材料以 4 ml/s 的率经由 antecubital 静脉被管理) MSCT 。每扫描上的 Precontrast 和 postcontrast 变细被学习。到主动脉的 SPN 的山峰高度的灌注,山峰高度,和比率被分析。灌注用时间密度曲线(TDC ) 和主动脉的山峰高度的最大的坡度被计算。量的参数(灌注,山峰高度,到主动脉的 SPN 的山峰高度的比率) 血流动,模式与 MVD 和 immunohistochemistry.Results 的 VEGF 表达式相比灌注山峰高度恶意((96. | BAI Rong-jie CHENG Xiao-guang QU Hui SHEN Bao-zhong HAN Ming-jun WU Zhen-hua | 2009 | Chinese Medical Journal2009，，5： | 19 |
| 8 | A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation显示文摘抄写因素(TF ) 的活动与特定的 DNA 序列和另外的规章的蛋白质要求相互作用。在 Arabidopsis 检测如此的相互作用,我们与 1589 Arabidopsis TF 的一个网关兼容机 Gal4-ADTF 图书馆开发了一个高产量的屏蔽系统,它能被基于配偶的 yeast-one-hybrid (Y1H ) 和 yeast-two-hybrid (Y2H ) 容易屏蔽方法。系统的效率被检验二个描绘得好的 TFDNA 和 TFprotein 相互作用验证:由由 Y2H 的 Y1H 和 NPR1TGAs 相互作用的 CHECCA1 倡导者相互作用。我们使用了这个系统识别与一个调停人子单元交往的八 TF, Med25,在 JA 发信号的一个关键管理者。我们识别了在 PDF1.2 的倡导者与 GCC 盒子 cis 元素交往了的五 TF, Med25 的下游的基因。我们发现都从 AP2-EREBP 家庭,三这些 TF 与 Med25 和 PDF1.2 的 GCC 盒子两个都直接交往,建议 Med25 调整通过这三 TF 的 PDF1.2 表示。这些结果证明屏蔽系统的这高产量的 Y1H/Y2H 是为在 Arabidopsis 学习 transcriptional 规定网络的一个有效工具。这个系统将为另外的 Arabidopsis 研究人员是可得到的,并且因此,它为 Arabidopsis 社区提供一个重要资源。 | Bin Oua Kang-Quan Yin Sai-Nan Liu Yan Yang Tren Gu Jennifer Man Wing Hui Li Zhang Jin Miao Youichi Kondou Minami Matsui Hong-Ya Gu Li-Jia Qu | 2011 | Molecular Plant2011，4，3： | 19 |
| 9 | Circular RNA PIP5K1A promotes colon cancer development through inhibiting mi R-1273a显示文摘BACKGROUND Circular RNAs (circRNAs) are considered to be highly stable due to the closed structure, which are predominately correlated with the development and progression of a wide variety of cancers. Colon cancer is one of the most common malignancies worldwide. A recent study demonstrated the upregulated expression of circPIP5K1A in non-small cell lung cancer. However, few studies have investigated the relationship between circ_0014130 level and colon cancer. Therefore, elucidating the underlying mechanisms of circPIP5K1A’s role may help with the identification of novel diagnostic and therapeutic targets for colon cancer. AIM To investigate the status of circPIP5K1A in colon cancers and its effects on the modulation of cancer development. METHODS The expression level of circPIP5K1A in tissue and serum samples from colon cancer patients, as well as human colonic cancer cell lines was detected by realtime quantitative reverse transcription-polymerase chain reaction. Following the transfection of specifically synthesized small interfering RNA (siRNA) into colon cell lines, we used Hoechst staining assay to measure the ratio of cell death in the absence of circPIP5K1A. Moreover, we also used the Transwell assay to assess the migratory function of colon cells overexpressing circPIP5K1A. Additionally, we employed a series of bioinformatics prediction programs to predict the potential of circPIP5K1A-targeted miRNAs and mRNAs. The miR-1273a vector was constructed, and then transfected with or without circPIP5K1A vector into colon cancer cells. Afterwards, the expression of activator protein 1 (AP-1), interferon regulating factor 4 (IRF-4), caudal type homeobox 2 (CDX-2), and zinc finger of the cerebellum 1 (Zic-1) was detected by western blotting. RESULTS CircPIP5K1A was significantly upregulated in colon cancer tissue relative to their adjacent normal tissues. Knockdown of circPIP5K1A in colon cancer cells impaired cell viability and suppressed cell invasion and migration, while enforced expression of circPIP5K1A exhibited the opposite effects on cell migration. Bioinformatics prediction program predicted that the association of circPIP5K1A with miR-1273a, as well as AP-1, IRF-4, CDX-2, and Zic-1. Subsequent studies showed that overexpression of circPIP5K1A augmented the expression of AP-1 but attenuated the expression of IRF-4, CDX-2, and Zic-1. Reciprocally, overexpression of miR-1273a abrogated the oncogenic function of circPIP5K1A in colon cancers. CONCLUSION Overall, our data demonstrate the oncogenic role of circPIP5K1A-miR-1273a axis in regulation of colon cancer development, which provides a novel insights into colon cancer pathogenesis. | Qu Zhang Chi Zhang Jian-Xin Ma Hui Ren Yu Sun Jiao-Zhen Xu | 2019 | World Journal of Gastroenterology2019，25，35： | 18 |
| 10 | Community structure and elevational diversity patterns of soil Acidobacteria显示文摘Acidobacteria is one of the most dominant and abundant phyla in soil,and was believed to have a wide range of metabolic and genetic functions. Relatively little is known about its community structure and elevational diversity patterns. We selected four elevation gradients from 1000 to 2800 m with typical vegetation types of the northern slope of Shennongjia Mountain in central China. The vegetation types were evergreen broadleaved forest,deciduous broadleaved forest,coniferous forest and sub-alpine shrubs. We analyzed the soil acidobacterial community composition,elevational patterns and the relationship between Acidobacteria subdivisions and soil enzyme activities by using the 16 S rRNA meta-sequencing technique and multivariate statistical analysis. The result found that 19 known subdivisions as well as an unclassified phylotype were presented in these forest sites,and Subdivision 6 has the highest number of detectable operational taxonomic units(OTUs). A significant single peak distribution pattern(P < 0.05) between the OTU number and the elevation was observed. The Jaccard and Bray–Curtis index analysis showed that the soil Acidobacteria compositional similarity significantly decreased(P < 0.01) with the increase in elevation distance. Mantel test analysis showed the most of the soil Acidobacteria subdivisions had the significant relationship(P < 0.01) with different soil enzymes. Therefore,soil Acidobacteria may be involved in different ecosystem functions in global elemental cycles. Partial Mantel tests and CCA analysis showed that soil pH,soil temperature and plant diversity may be the key factors in shaping the soil Acidobacterial community structure. | Yuguang Zhang Jing Cong Hui Lu Guangliang Li Yuanyuan Qu Xiujiang Su Jizhong Zhou Diqiang Li | 2014 | Journal of Environmental Sciences2014，26，8： | 17 |
| 11 | Experimental investigation of the generation of large-amplitude internal solitary wave and its interaction with a submerged slender body显示文摘A principle of generating the nonlinear large-amplitude internal wave in a stratified fluid tank with large cross-section is proposed according to the‘jalousie’control mode.A new wave-maker based on the principle was manufactured and the experiments on the generation and evolution of internal solitary wave were conducted.Both the validity of the new device and applicability range of the KdV-type internal soliton theory were tested.Furthermore,a measurement technique of hydrodynamic load of internal waves was developed.By means of accurately measuring slight variations of internal wave forces exerted on a slender body in the tank,their interaction characteristics were determined.It is shown that through establishing the similarity between the model scale in the stratified fluid tank and the full scale in the numerical simulation the obtained measurement results of internal wave forces are confirmed to be correct. | WEI Gang DU Hui XU XiaoHui ZHANG YuanMing QU ZiYun HU TianQun YOU YunXiang | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，2： | 17 |
| 12 | Vascular endothelial growth factor: an attractive target in the treatment of hypoxic/ischemic brain injury显示文摘Cerebral hypoxia or ischemia results in cell death and cerebral edema, as well as other cellular reactions such as angiogenesis and the reestablishment of functional microvasculature to promote recovery from brain injury. Vascular endothelial growth factor is expressed in the central nervous system after hypoxic/ischemic brain injury, and is involved in the process of brain repair via the regulation of angiogenesis, neurogenesis, neurite outgrowth, and cerebral edema, which all require vascular endothelial growth factor signaling. In this review, we focus on the role of the vascular endothelial growth factor signaling pathway in the response to hypoxic/ischemic brain injury, and discuss potential therapeutic interventions. | Hui Guo Hui Zhou Jie Lu Yi Qu Dan Yu Yu Tong | 2016 | Neural Regeneration Research2016，11，1： | 15 |
| 13 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 14 | Municipal wastewater treatment in China:Development history and future perspectives显示文摘China has the world's largest and still growing wastewater sector and water market,thus its future development will have profound influence on the world.The high-speed development of China's wastewater sector over the past 40 years has forged its global leading treatment capacity and innovation ability.However,many problems were left behind,including underdeveloped sewers and sludge disposal facilities,low sustainability of the treatment processes,questionable wastewater treatment plant(WWTP)effluent discharge standards,and lacking global thinking on harmonious development between wastewater management,human society and the nature.Addressing these challenges calls for fundamental changes in target design,policy and technologies.In this mini-review,we revisit the development history of China's municipal wastewater management and identify the remaining challenges.Also,we highlight the future needs of sustainable development and exploring China's own wastewater management path,and outlook the future from several aspects including targets of wastewater management,policies and technologies,especially the new concept WWTP.Furthermore,we envisage the establishment of new-generation WWTPs with the vision of turning WWTP from a site of pollutant removal into a plant of energy,water and fertilizer recovery and an integrated part urban ecology in China. | Jiuhui Qu Hongchen Wang Kaijun Wang Gang Yu Bing Ke Han-Qing Yu Hongqiang Ren Xingcan Zheng Ji Li Wen-Wei Li Song Gao Hui Gong | 2019 | Frontiers of Environmental Science & Engineering2019，13，6： | 13 |
| 15 | GGE biplot analysis of yield stability and test location representativeness in proso millet (Panicum miliaceum L.) genotypes显示文摘The experiments were conducted for three consecutive years across 14 locations using 9 non-waxy proso millet genotypes and 16 locations using 7 waxy proso millet genotypes in China. The objectives of this study were to analyze yield stability and adaptability of proso millets and to evaluate the discrimination and representativeness of locations by analysis of variance(ANOVA) and genotype and genotype by environment interaction(GGE) biplot methods. Grain yields of proso millet genotypes were significantly influenced by environment(E), genotype(G) and their interaction(G×E)(P<0.1%). G×E interaction effect was six times higher than G effect in non-waxy group and seven times in waxy group. N04-339 in non-waxy and Neimi 6(NM6) in waxy showed higher grain yields and stability compared with other genotypes. Also, Neimi 9(NM9, a non-waxy cultivar) and 90322-2-33(a waxy cultivar) showed higher adaptability in 7 and in 11 locations, respectively. For non-waxy, Dalat, Inner Mongolia(E2) and Wuzhai, Shanxi(E5) were the best sites among all the locations for maximizing the variance among candidate cultivars, and Yanchi, Ningxia(E10) had the best representativeness. Wuzhai, Shanxi(e9) and Yanchi, Ningxia(e14) were the best representative locations, and Baicheng, Jilin(e2) was better discriminating location than others for waxy genotypes. Based on our results, E10 and e14 have enhanced efficiency and accuracy for non-waxy genotypes and waxy genotypes selection, respectively in national regional test of proso millet varieties. | ZHANG Pan-pan SONG Hui KE Xi-wang JIN Xi-jun YIN Li-hua LIU Yang QU Yang SU Wang FENG Nai-jie ZHENG Dian-feng FENG Bai-li | 2016 | Journal of Integrative Agriculture2016，15，6： | 13 |
| 16 | Serum proteomics of early postoperative cognitive dysfunction in elderly patients显示文摘 | ZHANG Qing LI Shi-zhong FENG Chun-sheng QU Xiang-dong WANG Hui ZHANG Xue-na LIU Yang WANG Yun WU An-shi YUE Yun | 2012 | Chinese Medical Journal2012，，14： | 13 |
| 17 | Ribosome profiling analysis identified a KRAS-interacting microprotein that represses oncogenic signaling in hepatocellular carcinoma cells显示文摘The roles of concealed microproteins encoded by long noncoding RNAs(lncRNAs)are gradually being exposed,but their functions in tumorigenesis are still largely unclear.Here,we identify and characterize a conserved 99-amino acid microprotein named KRASIM that is encoded by the putative lncRNA NCBP2-AS2.KRASIM is differentially expressed in normal hepatocytes and hepatocellular carcinoma(HCC)cells and can suppress HCC cell growth and proliferation.Mechanistically,KRASIM interacts and colocalizes with the KRAS protein in the cytoplasm of human HuH-7 hepatoma cells.More importantly,the overexpression of KRASIM decreases the KRAS protein level,leading to the inhibition of ERK signaling activity in HCC cells.These results demonstrate a novel microprotein repressor of the KRAS pathway for the first time and provide new insights into the regulatory mechanisms of oncogenic signaling and HCC therapy. | Wenli Xu Bing Deng Penghui Lin Chang Liu Bin Li Qiaojuan Huang Hui Zhou Jianhua Yang Lianghu Qu | 2020 | Science China(Life Sciences)2020，63，4： | 11 |
| 18 | Cryptotanshinone suppresses key onco-proliferative and drugresistant pathways of chronic myeloid leukemia by targeting STAT5 and STAT3 phosphorylation显示文摘C-Myc and signal transducer and activator of transcription(STAT) family proteins have been proposed to be important downstream genes of BCR-ABL, which characterizes most cases of chronic myeloid leukemia(CML). Here, we report a c-Myc pathway-targeted screening of seven natural anticancer compounds, in which we identified cryptotanshinone as a highly promising agent for CML therapy. Cryptotanshinone depletes c-Myc in CML by repressing the phosphorylation of STAT5.Decreased viability of K562 cells correlated with p-STAT5 suppression. Unexpectedly, imatinib activates rather than inhibits the phosphorylation of STAT3 in K562 cells. We demonstrated that cryptotanshinone, as a dual inhibitor of p-STAT5 and p-STAT3,can effectively block IL-6-mediated STAT3 activation and reverse BCR-ABL kinase-independent drug resistance. Moreover, we showed that the epigenetic rebalance between decreased BCR-ABL/STAT5/c-Myc and enhanced STAT3/multi-drug resistance(MDR) pathways is characteristic of the cancer stem cell-like property of K562/ADR. Simultaneously suppressing these two pathways using cryptotanshinone proves to be critical for the malignant network redress and MDR reversal of K562/ADR. These studies reveal the dual functions of cryptotanshinone that suppress key oncogenic proliferation and drug-resistant pathways in CML cells by targeting p-STAT5 and p-STAT3, providing a new strategy for CML therapy that takes advantage of natural products. | Bowen Dong Zirui Liang Zhirong Chen Bin Li Lingling Zheng Jianhua Yang Hui Zhou Lianghu Qu | 2018 | Science China(Life Sciences)2018，61，9： | 10 |
| 19 | Monitoring early responses to irradiation with dual-tracer micro-PET in dual-tumor bearing mice显示文摘AIM: To monitor the early responses to irradiation in primary and metastatic colorectal cancer (CRC) with 18 Ffluorothymidine ( 18 F-FLT) and 18 F-fluorodeoxyglucose ( 18 F-FDG) small-animal position emission tomography (micro-PET). METHODS: The primary and metastatic CRC cell lines, SW480 and SW620, were irradiated with 5, 10 and 20, l l l , Gy. After 24 h, the cell cycle phases were analyzed. A dual-tumor-bearing mouse model of primary and metastatic cancer was established by injecting SW480 and SW620 cells into mice. micro-PET with 18 F-FLT and 18 F-FDG was performed before and 24 h after irradiation with 5, 10 and 20 Gy. The region of interest (ROI) was drawn over the tumor and background to calculate the ratio of tumor to non-tumor (T/NT) in tissues. Immunohistochemical assay and Western blotting were used to examine the levels of integrin β3, Ki-67, vascular endothelial growth factor receptor 2 (VEGFR2) and heat shock protein 27 (HSP27). RESULTS: The proportion of SW480 and SW620 cells in the G 2 -M phase was decreased with an increasing radiation dose. The proportion of SW480 cells in the G 0 -G 1 phase was increased from 48.33% ± 4.55% to 87.09% ± 7.43% (P < 0.001) and that of SW620 cells in the S-phase was elevated from 43.57% ± 2.65% to 66.59% ± 7.37% (P = 0.021). In micro-PET study, with increasing dose of radiation, 18 F-FLT uptake was significantly reduced from 3.65 ± 0.51 to 2.87 ± 0.47 (P = 0.008) in SW480 tumors and from 2.22 ± 0.42 to 1.76 ± 0.45 (P = 0.026) in SW620 tumors. 18 F-FDG uptake in SW480 and SW620 tumors was reduced but not significantly (F = 0.582, P = 0.633 vs F = 0.273, P = 0.845). Dose of radiation was negatively correlated with 18 F-FLT uptake in both SW480 and SW620 tumors (r = -0.727, P = 0.004; and r = -0.664, P = 0.009). No significant correlation was found between 18 F-FDG uptake and radiation dose in SW480 or SW620 tumors. HSP27 and integrin β3 expression was higher in SW480 than in SW620 tumors. The T/NT ratio for 18 F-FLT uptake was positively correlated with HSP27 and integrin β3 expression (r = 0.924, P = 0.004; and r = 0.813, P = 0.025). CONCLUSION: 18 F-FLT is more suitable than 18 F-FDG in monitoring early responses to irradiation in both primary and metastatic lesions of colorectal cancer. | Hui Wang, Jia-He Tian, Bai-Xuan Xu, Zhi-Wei Guan, ChangBin Liu, Rui-Min Wang, Ying-Mao Chen, Jin-Ming Zhang, Nuclear Medicine Department, The General Hospital of the Chinese People’s Liberation Army and Military Medical Postgraduate College, Beijing 100853, China Bo Liu, Department of Hepatobiliary Surgery, The General Hospital of the Chinese People’s Liberation Army and Military Medical Postgraduate College, Beijing 100853, China Bao-Lin Qu, Department of Radiation Oncology, The General Hospital of the Chinese People’s Liberation Army and Military Medical Postgraduate College, Beijing 100853, China | 2010 | World Journal of Gastroenterology2010，16，43： | 10 |
| 20 | Densification Mechanism of Warm Compaction and Powder Mixture Designing Rules显示文摘By phenomenological analysis of warm compaction, it is found that, compared with the contribution of particle plastical deformation to densification of powder compact,the particle rearrangement is a dominant densification mechanism for powder warm compaction, and the plastical deformation of particles plays an important role in offering accommodating deformation for particle rearrangement and densifying powder compact at the final stage of pressing.In order to attain density gain as high as possible during warm compaction, six rules for designing warm compacting powder mixtures were proposed in detail. | CAO Shun hua,HUANG Bai yun,QU Xuan hui,YI Jian hong(State Key Laboratory for Powder Metallurgy, Central South University of Technology, Changsha 410083, China) | 2000 | Journal of Central South University2000，12，1： | 10 |
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