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您的检索式：作者名="Pritesh Contractor"
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| 1 | Development of a sensitive and rapid method for quantitation of (S)-(-)- and (R)-(+)-metoprolol in human plasma by chiral LC–ESI–MS/MS显示文摘A selective, sensitive and high throughput liquid chromatography-tandem mass spectrometry(LC–ESI–MS/MS) method has been developed for separation and quantification of metoprolol enantiomers on a chiral Lux Amylose-2(250 mm 4.6 mm, 5 mm) column. Solid phase extraction of(S)-()- and(R)-(t)-metoprolol and rac-metoprolol-d6 as an internal standard(IS)was achieved on Lichrosep DVB HL cartridges employing 200 mL human plasma. Both the analytes were chromatographically separated with a resolution factor of 2.24 using 15 mM ammonium acetate in water, pH 5.0 and 0.1%(v/v) diethyl amine in acetonitrile(50:50, v/v) as the mobile phase within 7.0 min. The precursor-product ion transitions for the enantiomers and IS were monitored in the multiple reaction monitoring and positive ionization mode. The method was validated over the concentration range of 0.500–500 ng/mL for both the enantiomers. Matrix effect was assessed by post-column analyte infusion experiment and the mean extraction recovery was greater than 94.0% for both the enantiomers at all quality control levels. The stability of analytes was evaluated in plasma and whole blood under different storage conditions. The method was successfully applied to a clinical study in 14 healthy volunteers after oral administration of200 mg metoprolol tablet under fasting conditions. The assay reproducibility is shown by reanalysis of 68 incurred samples. The suitability of the developed method was assessed in comparison | Primal Sharma Pritesh Contractor Swati Guttikar Daxesh P.Patel Pranav S.Shrivastav | 2014 | Journal of Pharmaceutical Analysis2014，4，1： | 3 |
| 2 | Determination of ergocalciferol in human plasma after Diels-Alder derivatization by LC-MS/MS and its application to a bioequivalence study显示文摘An accurate, sensitive and selective method is developed for determination of ergocalciferol(vitamin D_2) in human plasma using LC–MS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione(PTAD), a strong dienophile based on Diels-Alder reaction.Ergocalciferol and its deuterated internal standard, ergocalciferol-d6, were analyzed on X Select CSH C_(18)(100 mm×4.6 mm, 2.5 μm) column using acetonitrile and 0.1%(v/v) formic acid in water containing 0.14% methylamine within 6.0 min under gradient elution mode. Tandem mass spectrometry in positive ionization mode was used to quantify ergocalciferol by multiple reaction monitoring(MRM). Entire data processing was done using Watson LIMS^(TM) software which provided excellent data integrity and high throughput with improved operational efficiency. The major advantage of this method includes higher sensitivity(0.10 ng/mL),superior extraction efficiency(≥83%) and small sample volume(100 μL) for processing. The method was linear in the concentration range of 0.10–100 ng/mL for ergocalciferol. The intra-batch and inter-batch accuracy and precision(% CV) values varied from 97.3% to 109.0% and 1.01% to 5.16%, respectively. The method was successfully applied to support a bioequivalence study of 1.25 mg ergocalciferol capsules in 12 healthy subjects. | Pritesh Contractor Abhishek Gandhi Gajendra Solanki Priyanka A. Shah Pranav S. Shrivastav | 2017 | Journal of Pharmaceutical Analysis2017，7，6： | 1 |
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