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| 1 | 北极冻土区活跃层与永冻层土壤微生物组的空间分异显示文摘【目的】研究北极地区表层季节性融解冻土(活跃层)及埋藏于其下深层永久冻土(永冻层)的土壤呼吸速率、土壤微生物组差异和活性甲烷氧化微生物。【方法】在相距2700 km的挪威斯瓦尔巴群岛和俄罗斯西伯利亚典型冻土区,共获得4个活跃层及4个永冻层土壤。模拟北极夏季近原位温度(10°C)培养土壤样品,测定土壤呼吸强度;利用稳定性同位素^(13)CH_4示踪土壤甲烷氧化微生物核酸DNA;结合高通量测序16S rR NA基因,实时荧光定量qPCR及土壤理化性质分析,研究活跃层和永冻层土壤微生物群落差异及其对土壤呼吸的影响,揭示活性甲烷氧化微生物的群落组成。【结果】西伯利亚冻土区土壤呼吸速率明显高于挪威斯瓦尔巴岛地区,其平均速率相差高达17倍。冻土区活跃层呼吸速率高于永冻层,活跃层约为61–7293 nmol CO_2/(g dws·d),而永冻层约为47–523 nmol CO_2/(g dws·d)。相应的,在所有活跃层中均发现变形菌和酸杆菌门共计10个微生物科的丰度显著高于永冻层,其中Hyphomicrobiaceae、Solibacteraceae和Sinobacteraceae是优势科,在活跃层中的相对丰度约为4.3%–18.6%,是永冻层的2.6–23.7倍,这些微生物可能是活跃层土壤呼吸强度较高的主要原因。稳定性同位素^(13)CH_4示踪仅发现西伯利亚冻土活跃层能够氧化高浓度甲烷,其中的活性甲烷碳同化微生物为Methylobacterium和Crenothrix。【结论】北极冻土区土壤微生物组存在明显的空间分异规律,并能较好解释土壤呼吸强度变化特征,而活跃层和永冻层垂直深度及其可能引起的物理化学因子可能是冻土区微生物组演替的主要环境驱动力。未来全球变暖情景下,永冻层逐渐融解并形成活跃层,其中的功能微生物将可能经历定向演替,并在北极冻土碳转化中发挥重要作用。 | 张慧敏 Anders Priemé Samuel Faucherre Bo Elberling 贾仲君 | 2017 | 微生物学报2017，57，6： | 3 |
| 2 | Distributed ledger technology for securities clearing and settlement: benefits, risks, and regulatory implications显示文摘This article outlines the benefits and risks of the distributed ledger technology(DLT)for the clearing and settlement of exchange-traded and OTC securities,followed by a description of the technology’s potential role for central counterparties and central securities depositories.Although the industry and scholars are attempting to solve the technological and operational issues that DLT systems still face,outstanding legal risks are such that the financial industry is asking for more regulatory guidance and intervention.This article wants to contribute to the public policy debate by presenting potential regulatory barriers that may have to be removed for DLT to be fully adopted.In addition,it identifies areas requiring an update of the legal framework in order to address certain prudential and conduct risks that this technology could introduce. | Randy Priem | 2020 | Financial Innovation2020，6，1： | 2 |
| 3 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 4 | Virulence and pathogenesis of non-virulent and virulent strains of pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus显示文摘 | Mulder W A Priem J Glazenburg K L | 1994 | J Gen Virol1994，75，1： | 1 |
| 5 | Insights and new direc- tions from demand-side approaches to technology inno- vation, entrepreneurship, and strategic management re- search显示文摘 | Priem R L Li S Carr J C | 2012 | Journal of Management2012，38，1： | 1 |
| 6 | Genotype and phenotype of glutathione S-transferase mu in testieular cancer patients 显示文摘 | Vistisen K Prieme H Okkels H | 1997 | Pharmacogenetics1997，7，1： | 1 |
| 7 | Virulence and pathogenesis of non-virulent and virulent strains of pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus显示文摘 | Mulder W A Priem J Glazenburg K L | 1994 | Journal of General Virology1994，75，1： | 1 |
| 8 | On the thermal stability of Rb-Sr and K-Ar biotite systems: Evidence from coexisting Sveconorwegian (ca 870 Ma) and Caledonian (ca 400 Ma) biotites in SW Norway显示文摘 | R. H. Verschure P. A. M. Andriessen N. A. I. M. Boelrijk E. H. Hebeda C. Maijer H. N. A. Priem E. A. Th. Verdurmen | 1980 | Contributions to Mineralogy and Petrology1980，，3： | 1 |
| 9 | Curcumin inhibits pro-inflammatory mediators and metalloproteinase- 3 production by chondrocytes 显示文摘 | MATHY- HARTERT M JACQUEMOND- COLLET I PRIEM F | 2009 | Inflamm Res2009，58，12： | 1 |
| 10 | β-Trace protein,cystatin C,β2-microglobulin,and creatinine compared for detecting impaired glomerular filtration rates in children显示文摘 | Filler G Priem F Lepage N | 2002 | Clin Chem2002，48，5： | 1 |
| 11 | Identification of prion protein gene polymorphisms in goats from italian scrapie outbreaks 显示文摘 | ACUTIS P L BOSSERS kA PRIEM J V | 2006 | J Gen Viro12006，87，4： | 1 |
| 12 | Relationship between semen quality and the seminal plasma components carnitine,alpha-glucosidase,fructose,citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，4： | 1 |
| 13 | Top Management Team Trust, Behavioral Integration and the Performance of International Joint Ventures 显示文摘 | Liang X Priem R Shaffer M | 2013 | Journal of Asia Business Studies2013，7，2： | 1 |
| 14 | Natural perturbations, drying-wetting and freezing-thawing cycles, and the emission of nitrous oxide, carbon dioxide and methane from farmed organic soils显示文摘 | Prieme A Christensen S | 2001 | Soil Biology and Biochemistry2001，33，15： | 1 |
| 15 | Is the resource-based 'view' a useful perspective for strategic management research?显示文摘 | Priem R L Butler J E | 2001 | Academy of Management Review2001，26，1： | 1 |
| 16 | Relationship between semen quality and the seminal plasma components carnitine, alpha-glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population 显示文摘 | Zopfgen A Priem F Sudhoff F | 2004 | Hum Reprod2004，15，4： | 1 |
| 17 | Endothelin receptor A blockade reduces proteinuria and vascular hypertrophy in spontaneously hypertensive rats on high-salt diet in ablood-pressure-independent manner显示文摘 | Trenkner J Priem F Bauer C | 2002 | Clin Sci (Lond)2002，，： | 1 |
| 18 | 显示文摘 | Schimke l Muller J Priem F | 2001 | J Am Coll Cardiol2001，38，1： | 1 |
| 19 | Routine workflow for useof urine strips and urine flow cytometer UF-100 in the hospital laboratory显示文摘 | Lun A Ziebig R Priem F | 1999 | Clin Chem1999，45，81： | 1 |
| 20 | Relationship between semen quality and the seminal plasma components carnitine, alpha glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，4： | 1 |
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