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| 1 | 新型STAT3信号转导通路抑制剂WP1193在体外对脑肿瘤干细胞生物学特性影响的研究显示文摘背景与目的:恶性脑胶质母细胞瘤(glioblastoma Multiforme,GBM)是最常见的成人原发性脑肿瘤,预后仍不理想。近年来,肿瘤干细胞理论认为脑肿瘤干细胞是GBM进展及治疗耐受的主要原因,只有针对脑肿瘤干细胞的靶向治疗才能更加有效的治疗GBM。本研究旨在探讨新型STAT3信号转导通路抑制剂WP1193在体外对脑肿瘤干细胞生物学特性的影响。方法:从新鲜手术切除的GBM标本中,分离、培养及鉴定脑肿瘤干细胞。采用Western blot及RT-PCR法检测WP1193给药后,STAT3信号转导通路的变化。使用神经球形成实验评估WP1193对GBM干细胞形成神经球能力的影响。利用RT-PCR及流式细胞技术检测WP1193对CD133阳性细胞的影响。采用MTS及PI染色结合流式细胞技术检测WP1193对GBM干细胞增殖及细胞周期的影响。采用AnnexinV流式细胞术分析WP1193对GBM干细胞的凋亡诱导效应。结果:WP1193抑制GBM干细胞STAT3磷酸化及下游基因的表达。WP1193有效抑制GBM干细胞形成神经球的能力及增殖,并可将细胞阻滞于G1期。WP1193在体外通过改变Bax/Bcl-2比例诱导GBM干细胞凋亡。结论:WP1193通过抑制STAT3信号转导通路,有效的抑制GBM干细胞的增殖及形成神经球的能力,并能诱导凋亡。STAT3信号转导通路可作为GBM干细胞的治疗靶点。 | 赛克 WK Alfred Yung Fred Lang Waldemar Priebe Howard Colman 陈忠平 | 2009 | 中国神经肿瘤杂志2009，7，3： | 3 |
| 2 | Colorectal cancer biomarkers: To be or not to be? Cautionary tales from a road well travelled显示文摘Colorectal cancer(CRC)is the second most common cause of cancer-related death worldwide and places a major economic burden on the global health care system.The time frame for development from premalignant to malignant disease typically spans 10-15 years,and this latent period provides an ideal opportunity for early detection and intervention to improve patient outcomes.Currently,early diagnosis of CRC is hampered by a lack of suitable non-invasive biomarkers that are clinically or economically acceptable for populationbased screening.New blood-based protein biomarkers for early detection of CRC are therefore urgently required.The success of clinical biomarker discovery and validation studies is critically dependent on understanding and adjusting for potential experimental,analytical,and biological factors that can interfere with the robust interpretation of results.In this review we outline some important considerations for research groups undertaking biomarker research with exemplars from our studies.Implementation of experimental strategies to minimise the potential effects of these problems will facilitate the identification of panels of biomarkers with the sensitivity and specificity required for the development of successful tests for the early detection and surveillance of CRC. | Kim YC Fung Edouard Nice Ilka Priebe Damien Belobrajdic Aloke Phatak Leanne Purins Bruce Tabor Celine Pompeia Trevor Lockett Timothy E Adams Antony Burgess Leah Cosgrove | 2014 | World Journal of Gastroenterology2014，20，4： | 2 |
| 3 | STAT3信号转导通路抑制剂诱导胶质瘤干细胞产生自噬的研究显示文摘背景与目的:胶质瘤干细胞(glioma stem cell,GSC)在胶质瘤发展及治疗抗拒中发挥重要作用。我们以往的研究表明,新型STAT3信号转导通路抑制剂(STAT3 inhibitor,STI)WP1193能够诱导GSC产生细胞周期阻滞及凋亡。本研究旨在探讨STI是否能在体外诱导GSC产生自噬现象。方法:从手术切除的胶质母细胞瘤中分离及培养GSC。使用STI处理GSC。利用细胞计数法检测STI对GSC增殖的影响。使用Western blot检测自噬相关蛋白LC3的表达情况。吖啶橙染色后,利用荧光显微镜及流式细胞技术检测酸性自噬小体。使用透射电镜检测GSC中自噬小体。结果:STI剂量依赖性的抑制GSC的增殖。STI处理后,GSC中出现LC3表达的切换。STI处理后,GSC中出现自噬小体,且出现自噬小体细胞的比例增加。结论:STI能够在GSC中诱导自噬现象的产生。自噬在STI治疗中的意义及调节机制需要进一步的研究。 | 赛克 WK Alfred Yung Howard Colman Waldemar Priebe 陈忠平 | 2011 | 中国神经肿瘤杂志2011，9，2： | 2 |
| 4 | Triggers of perioperafive myocardial ischaemia and infarction显示文摘 | Priebe HJ | 2004 | Br J Anaesth2004，93，： | 1 |
| 5 | Testicular calcification in a 4-year-old boy显示文摘 | Priebe CJ Jr Garret R | 1970 | Pedistries1970，46，5： | 1 |
| 6 | High - frequency oscillatory ventilation in pediatric patients显示文摘 | Priebe G P Arnold J H | 2001 | Respir Care Clin N Am2001，7，： | 1 |
| 7 | Anaesthetic management for hip arthroplasty in a 46-yr-old patient with uncorrected truncus arteriosus type IV显示文摘 | FISCHER M U PRIEBE H J | 2006 | Br J Anaesth2006，97，3： | 1 |
| 8 | Patient’s experiences of involuntaryhospital admission and treatment ? a review of qualitative stud-ies显示文摘 | Katsakou C Priebe S | 2007 | Epidemiol Psichiatr Soc2007，16，2： | 1 |
| 9 | Aneurysmal subarachnoid haemorrhage and the anaesthetist显示文摘 | Priebe HJ | 2007 | Br J Anaesth2007，99，1： | 1 |
| 10 | Topical neutralization of IL-17 during experimental Pseudomonas aeruginosa corneal infection promotes bacterial clearance and reduces pathology显示文摘 | Zaidi TS Zaidi T Pier GB Priebe GP | | 0，，： | 1 |
| 11 | The effects of subchronic haloperidol on intact and dizocilpine-disrupted sensorimotor gating 显示文摘 | Feifel D Priebe K | 1999 | Psychopharmacology ( Berl)1999，146，2： | 1 |
| 12 | Mechanisms of direction selectivity in cat primary visual cortex as revealed by visual adaptation 显示文摘 | Priebe N J Lampl I Ferster D | 2010 | J Neurophysiol2010，104，5： | 1 |
| 13 | Supporting attribute?based access control in authorization and authentication infra?structures with ontologies显示文摘 | Priebe T Dobmeier W Schlager C | 2007 | Journal of Software2007，2，1： | 1 |
| 14 | Inescapable need for neutrophils as mediators of cellular innate immunity to acute Pseudomonas aeruginosa pneumonia显示文摘 | Koh A Y Priebe G P Ray C | 2009 | Infect Im- mun2009，77，12： | 1 |
| 15 | Mental Disorders, Psycho- logical Symptoms and Quality of Life 8 Years after an Earthquake: Findings from a Community Sample in Italy显示文摘 | Priebe S Marchi F Bini L | 2011 | Soc Psychiatry Psychiatr Eoidemiol2011，46，7： | 1 |
| 16 | The role of colonic metabolism in lactose intolerance显示文摘 | He T Venema K Priebe M G | 2008 | European Journal of Clinical Investigation2008，38，8： | 1 |
| 17 | Intravascular volume replacement therapy with synthetic colloids: Is there an influence on renal function? 显示文摘 | Boldt J Priebe HJ | 2003 | Anesth Analg2003，96，2： | 1 |
| 18 | Primary melanoma of small intestine masquerading as gastrointestinal stromal tumor: a case report and literature review 显示文摘 | Gabali AM Priebe P Ganesan S | 2008 | Am Surg2008，74，4： | 1 |
| 19 | Abschatzung des setmangsverhaltens eines durch stopfverdichtung verbesserten baugrundes显示文摘 | PRIEBE H J | 1976 | Die Bautechnik1976，53，5： | 1 |
| 20 | 2012 cervical cancer screening guidelines and the future role of HPV testing显示文摘 | Priebe A M | 2013 | Clin Obstet Gynecol2013，56，1： | 1 |
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