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| 1 | Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand显示文摘AIM: The role of hepatitis B virus (HBV) genotypes on the clinical features and prognosis of patients with hepatocellular carcinoma (HCC) is currently unknown. The aim of the present study was to evaluate the distribution of HBV genotypes and their clinical relevance in Thai patients.METHODS: HBV genotypes were determined by PCR-RFLP in stored sera of 93 asymptomatic carriers, 103 patients with chronic hepatitis, 60 patients with cirrhosis and 76patients with HCC. The clinical data were analyzed in relation to the HBV genotype.RESULTS: HBV genotypes C and B were predominant in Thailand, accounting for 73% and 21%, respectively. The distributions of genotypes B and C were similar in HCC patients compared to the other groups. Genotype C was significantly more common in HCC patients who were under 40 years old than genotype B (18% vs 0%, P= 0.03), but was significantly less common in patients older than 60 years (26% vs 56.5%, P= 0.01). The positive rate of hepatitis B e antigen (HBeAg) in patients with genotype C was significantly higher than that in patients with genotype B (71.6% vs 44.4%, P = 0.03 in chronic hepatitis; 56.8% vs 11.1%,P = 0.01 in cirrhosis). There were no differences between HCC patients with genotypes B and C regarding tumor staging by CLIP criteria and the overall median survival. Multivariate analyses showed that HBV genotype was not an independent prognostic factor of survival in HCC patients.CONCLUSION: Patients with genotype C had a higher positive rate of HBeAg and exhibited earlier progression of cirrhosis and HCC than those with genotype B. However,there were no differences in the risk of developing HCC and its prognosis between patients with these genotypes. | Pisit Tangkijvanich Varocha Mahachai Piyawat Komolmit Juthatip Fongsarun Apiradee Theamboonlers Yong Poovorawan | 2005 | World Journal of Gastroenterology2005，11，15： | 17 |
| 2 | 臭灵丹水提取物的急性毒性及镇痛作用的实验研究(英文)显示文摘采用上下移动法,醋酸扭体法、热板法、福尔马林致痛试验对臭灵丹水提取物的急性毒性及镇痛作用进行了研究。结果显示:臭灵丹水提取物LD50(腹腔注射)为1.19g/kg;臭灵丹水提取物明显抑制醋酸所致的小鼠扭体数,显著减少福尔马林致痛试验后期小鼠舔足行为;而对热板法所致疼痛无明显作用。表明臭灵丹水提取物具有一定的外周镇痛作用。 | 赵永娜 Reanmongkol Wantana Bouking Pisit 张荣平 | 2005 | 天然产物研究与开发2005，17，4： | 16 |
| 3 | Role of serum interleukin-18 as a prognostic factor in patients with hepatocellular carcinoma显示文摘AIM:To determine whether serum interleukin-18 (IL-18) levels correlated with clinicopathologic features and prognosis in patients with hepatocellular carcinoma (HCC). METHODS:Serum IL-18,IL-6 and IL-12 levels were measured by enzyme-linked immunosorbent assay (ELISA) from 70 patients with HCC and 10 healthy controls. RESULTS:Serum IL-18,IL-6 and IL-12 levels of patients with HCC were significantly higher that those of the controls. The levels of IL-18 correlated significantly with the presence of venous invasion and advanced tumor stages classified by Okuda's criteria. Patients with high serum IL-18 levels (≥ 105 pg/mL) had a poorer survival than those with low serum IL-18 levels (< 105 pg/mL) (4 and 11 mo,respectively,P = 0.015). Multivariate analyses showed that serum IL-18 level,but not IL-6 and IL-12 levels,was a significant and independent prognostic factor of survival. CONCLUSION:These findings demonstrate that serum IL-8 may a useful biological marker of tumor invasiveness and an independent prognostic factor of survival for patients with HCC. Thus,the detailed mechanisms of IL-18 involving in tumor progression should be further investigated. | Pisit Tangkijvanich Duangporn Thong-ngam Varocha Mahachai Apiradee Theamboonlers Yong Poovorawan | 2007 | World Journal of Gastroenterology2007，13，32： | 15 |
| 4 | Interleukin-1β gene polymorphism associated with hepatocellular carcinoma in hepatitis B virus infection显示文摘AIM:To examine the effect of interleukin-l-beta (IL-1β)promoter region C-511T and IL-1 receptor antagonist(IL-1RN) polymorphism among the patients with chronichepatitis B virus (HBV) infection (HCC and non-HCC).METHODS:Genomic DNA from 136 Thai patients withchronic HBV infection (HCC=46 and non-HCC=90) and152 healthy individuals was genotyped for IL-1β genepolymorphism (-511) using polymerase chain reactionwith sequence specific primers (PCR-SSP).The variablenumber of tandem repeats (VNTR) of IL-1RN gene wasassessed by a PCR-based assay.The association betweenthese genes and status of the disease was evaluated byX^2 test.RESULTS:IL-1B-511 genotype C/C was found tobe significantly different in patients with HCC whencompared with healthy individuals (P=0.036,OR=2.29,95%CI=1.05-4.97) and patients without HCC (P=0.036,OR=2.52,95%CI=1.05-6.04).Analysis of allelefrequencies of IL-1B-511 showed that IL-1B-511 Callele was also significantly increased in patients withHCC,compared to that in healthy control (P=0.033,OR=1.72,95%CI=1.04-2.84).However,no significantassociation in IL-1RN gene was found between the twogroups.CONCLUSION:IL-1B-511C allele,which may beassociated with high IL-1B production in the liver,is agenetic marker for the development of HCC in chronic hepatitis B patients in Thai population. | Nattiya Hirankarn Ingorn Kimkonq Pittaya Kummee Pisit Tanqkijyanich Yong Poovorawan | 2006 | World Journal of Gastroenterology2006，12，5： | 13 |
| 5 | Diagnostic role of serum interleukin-18 in gastric cancer patients显示文摘瞄准:在胃的癌症病人的各种各样的方面决定当前的地位并且在泰国病人与浆液 interleukins 的预示的角色发现临床的关联。方法:68 个病人在 2003 年 4 月期间在楚拉隆克恩国王纪念物医院在这研究被注册到 2005 年 5 月。胃的癌症组织学地在 17 个病人在 51 个病人和胃溃疡被证明。浆液 IL-6, IL-10, IL-12,和 IL-18 层次被连接酶的免疫吸着剂试金(ELISA ) 测量。结果:有 26 男性(55.32%) 和 21 女性(44.68%) ,他们的年龄从 33 ~ 85 年(意味着年龄 64.49 +/- 13.83 年) 。普通演讲是重量损失(41.2%) ,消化不良(39.2%) ,并且上面的胃肠的流血 15.7%) 。35.3% 胃的癌症病人的一个总数并且 6.3% 胃溃疡病人是吸烟者(P = 0.029 ) 。而且, 32.4% 胃的癌症病人和 6.3% 胃溃疡病人是含酒精的喝酒者(P = 0.044 ) 。损害地点在 39.4% 是幽门窦的,在 39.4% 的胃的身体,在 12.2% 的上面的胃和在 6.1% 病人的全部胃。H pylori 感染在 44.4% 被检测。糟糕区分的腺癌是最普通的病理学的发现(60.7%) 。外科疗法在 44.1% 病人被执行(在 5.9% 的全胃切除术,在 32.4% 的大部胃切除术和 palliative 在 5.9% 绕过外科) 。全身的化疗在 8.8% 病人作为辅助治疗被给。癌病 peritoneii 在 18.8% 病人被发现。吝啬的生存时间是 13.03 +/- 9.75 瞬间。在胃的癌症病人组的 IL-18 水平(58.54 +/- 43.96 pg/mL ) 在胃溃疡病人组比那显著地高(30.84 +/- 11.18 pg/mL )(P = 0.0001 )(95% CI 是 42.20, 13.19 ) 。为胃的癌症的诊断的 IL-18 的伤口点是 40 pg/mL,积极预兆的价值是 92.31% 。在有远转移的胃的癌症病人的 IL-6 水平(20.21 +/- 9.37 pg/mL ) 在没有转移的那些比那显著地高(10.13 +/- 7.83 pg/mL )(P = 0.037 )(95% CI 是 19.51, 0.65 ) 。在胃的癌症病人的 IL-10 和 IL-12 层次的角色是没有有效差量提供数据。结论:这些调查结果表明那浆液 IL-6 和 IL-18,然而并非 IL-10 和 IL-12 层次可以在有胃的癌症的病人是临床的关联和预示的因素的有用生物标记。而且, IL-18 能与高积极的预兆的价值为胃的癌症用作一个诊断标记。 | Duangporn Thong-Ngam Pisit Tangkijvanich Rungsun Lerknimitr Varocha Mahachai Apiradee Theamboonlers Yong Poovorawan | 2006 | World Journal of Gastroenterology2006，12，28： | 13 |
| 6 | Increased ATG5-ATG12 in hepatitis B virus-associated hepatocellular carcinoma and their role in apoptosis显示文摘AIM To investigate autophagy-related genes, particularly ATG12, in apoptosis and cell cycle in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) and non-HBV-HCC cell lines.METHODS The expression of autophagy-related genes in HBVassociated hepatocellular carcinoma and non-HBV-HCC cell lines and human liver tissues was examined by quantitative real-time reverse transcriptase-polymerase chain reaction(q RT-PCR) and western blotting. The silencing of target genes was used to examine the function of various genes in apoptosis and cell cycle progression. RESULTS The expression of autophagy related genes ATG5, ATG12, ATG9 A and ATG4 B expression was analyzed in Hep G2.2.15 cells and compared with Hep G2 and THLE cells. We found that ATG5 and ATG12 m RNA expression was significantly increased in Hep G2.2.15 cells compared to HepG 2 cells(P < 0.005). Moreover, ATG5-ATG12 protein levels were increased in tumor liver tissues compared to adjacent non-tumor tissues mainly from HCC patients with HBV infection. We also analyzed the function of ATG12 in cell apoptosis and cell cycle progression. The percentage of apoptotic cells increased by 11.4% in ATG12-silenced Hep G2.2.15 cells(P < 0.005) but did not change in ATG12-silenced HepG 2 cells under starvation with Earle's balanced salt solution. However, the combination blockade of Notch signaling and ATG12 decreased the apoptotic rate of HepG 2.2.15 cells from 55.6% to 50.4%(P < 0.05). CONCLUSION ATG12 is important for HBV-associated apoptosis and a potential drug target for HBV-HCC. Combination inhibition of ATG12/Notch signaling had no additional effect on HepG 2.2.15 apoptosis. | Areerat Kunanopparat Ingorn Kimkong Tanapat Palaga Pisit Tangkijvanich Boonchoo Sirichindakul Nattiya Hirankarn | 2016 | World Journal of Gastroenterology2016，22，37： | 11 |
| 7 | Gender difference in clinicopathologic features and survival of patients with hepatocellular carcinoma显示文摘AIM: To determine the influence of gender on the clinicopathologic characteristics and survival of patients with hepatocellular carcinoma (HCC). METHODS: A retrospective analysis of medical records was performed in 299 patients with HCC and their clinicopathologic features and survival were compared in relation to gender. RESULTS: There were 260 male (87%) and 39 female patients (13%), with a male-to-female ratio of 6.7:1. Female patients had lower mean serum bilirubin levels (P=O.03), lower proportion of alcohol abuse (P=O.O02), smaller mean tumor size (P=O.02), more frequent nodular type but less frequent massive and diffuse types of HCC (P=O.01), wereless advanced in Okuda's staging (P=O.04), and less frequently associated with venous invasion (P=O.03). The median survivals in females (14 mo) were significantly longer than that of male patients (4 mo) (P=O.O04, log-rank test). Multivariate analysis demonstrated that high serum alphafetoprotein levels, venous invasion, extrahepatic metastasis and lack of therapy were independent factors related to unfavorable prognosis. However, gender did not constitute a predictive variable associated with patient survival. CONCLUSION: Female patients tend to have higher survival rates than males. These differences were probably due to more favorable pathologic features of HCC at initial diagnosis and greater likelihood to undergo curative therapy in female patients. | Pisit Tangkijvanich Varocha Mahachai Pongspeera Suwangool Yong Poovorawan | 2004 | World Journal of Gastroenterology2004，10，11： | 9 |
| 8 | Hepatitis C virus genotype 6:Virology,epidemiology,genetic variation and clinical implication显示文摘Hepatitis C virus(HCV)is a serious public health problem affecting 170 million carriers worldwide.It is a leading cause of chronic hepatitis,cirrhosis,and liver cancer and is the primary cause for liver transplantation worldwide.HCV genotype 6(HCV-6)is restricted to South China,South-East Asia,and it is also occasionally found in migrant patients from endemic countries.HCV-6 has considerable genetic diversity with23 subtypes(a to w).Although direct sequencing followed by phylogenetic analysis is the gold standard for HCV-6 genotyping and subtyping,there are also now rapid genotyping tests available such as the reverse hybridization line probe assay(INNO-LiPAⅡ;Innogenetics,Zwijnaarde,Belgium).HCV-6 patients present with similar clinical manifestations as patients infected with other genotypes.Based on current evidence,the optimal treatment duration of HCV-6 with pegylated interferon/ribavirin should be 48 wk,although a shortened treatment duration of 24 wk could be sufficient in patients with low pretreatment viral load who achieve rapid virological response.In addition,the development of direct-acting antiviral agents is ongoing,and they give high response rate when combined with standard therapy.Herein,we review the epidemiology,classification,diagnosis and treatment as it pertain to HCV-6. | Vo Duy Thong Srunthron Akkarathamrongsin Kittiyod Poovorawan Pisit Tangkijvanich Yong Poovorawan | 2014 | World Journal of Gastroenterology2014，20，11： | 4 |
| 9 | Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b显示文摘AIM: To study the differential protein profile in serum of hepatitis B patients.METHODS: Serum samples were obtained from patients with chronic hepatitis B who were receiving peginterferon alfa-2b.The serum samples were subjected to albumin depletion and analyzed by two-dimensional gel electrophoresis(2-DE).Differentially expressed protein spots were identified by electrospray ionizationquadrupole time-of-flight mass spectrometry.Alpha2-HS-glycoprotein,complement component C3c and CD5 antigen were further analyzed by an enzymelinked immunosorbent assay and immunonephelometry.RESULTS: Nineteen patients with HBeAg-positive chronic hepatitis B(CHB) were studied.These patients were followed for at least 1 year after treatment and were classified according to their treatment response: responders(n = 9) and non-responders(n = 10).2-DE and MS/MS analysis were performed to compare the serum proteins before initiating peginterferon alfa2b.From the quantitative analysis of the 2-D gel,7 proteins were detected between the two groups at different levels before treatment.Among these potential candidates,serum levels of alpha-2-HS-glycoprotein,complement component C3c and CD5 antigen-like precursor were further analyzed.In the validation phase,23 subjects,9 sustained responders and 14 nonresponders,were recruited.Interestingly,the levels of alpha-2-HS-glycoprotein and complement component C3c were elevated in the serum of the non-responders compared to the responders.CONCLUSION: Serum alpha-2-HS-glycoprotein and complement component C3c may be potential serum biomarkers in predicting the treatment response of peginterferon alfa-2b in patients with CHB prior to treatment. | Sunida Kuakarn Poorichaya SomParn Pisit Tangkijvanich Varocha Mahachai Visith Thongboonkerd Nattiya Hirankarn | 2013 | World Journal of Gastroenterology2013，19，31： | 4 |
| 10 | Delta-like ligand 4 in hepatocellular carcinoma intrinsically promotes tumour growth and suppresses hepatitis B virus replication显示文摘AIM To investigate the role of Delta-like ligand 4(DLL4) on tumour growth in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) in vivo.METHODS We suppressed DLL4 expression in an HBV expressing HCC cell line, HepG2.2.15 and analysed the growth ability of cells as subcutaneous tumours in nude mice. The expression of tumour angiogenesis regulators, VEGF-A and VEGF-R2 in tumour xenografts were examined by western blotting. The tumour proliferation and neovasculature were examined by immunohistochemistry. The viral replication and viral protein expression were measured by quantitative PCR and western blotting, respectively.RESULTS Eighteen days after implantation, tumour volume in mice implanted with sh DLL4 HepG2.2.15 was significantly smaller than in mice implanted with control HepG2.2.15(P < 0.0001). The levels of angiogenesis regulators, VEGF-A and VEGF-R2 were significantly decreased in implanted tumours with suppressed DLL4 compared with the control group(P < 0.001 and P < 0.05, respectively). Furthermore, the suppression of DLL4 expression in tumour cells reduced cell proliferation and the formation of new blood vessels in tumours. Unexpectedly, increased viral replication was observed after suppression of DLL4 in the tumours.CONCLUSION This study demonstrates that DLL4 is important in regulating the tumour growth of HBV-associated HCC as well as the neovascularization and suppression of HBV replication. | Areerat Kunanopparat Jiraphorn Issara-Amphorn Asada Leelahavanichkul Anapat Sanpavat Suthiluk Patumraj Pisit Tangkijvanich Tanapat Palaga Nattiya Hirankarn | 2018 | World Journal of Gastroenterology2018，24，34： | 3 |
| 11 | Direct measurement of gastric H^+/K^+-ATPase activities in patients with or without Helicobacter pylori-associated chronic gastritis显示文摘AIM: The role of Helicobacter pylori (H pylori) infection in gastric acid secretion of patients with chronic gastritisremains controversial. This study was designed to elucidate the effect of H pylori on H+/K+-ATPase activities in gastric biopsy specimens.METHODS: Eighty-two patients with chronic gastritis who had undergone upper endoscopy were included in this study. H pylori infection was confirmed by rapid urease test and histology. Gastric H+/K+-ATPase activities and serum gastrin concentrations were measured by an enzymatic method and radioimmunoassay, respectively. For those patients who received triple therapy for eradicating H pylori, changes in the activity of gastric H+/K+-ATPase and serum gastrin levels were also measured. RESULTS: The mean gastric H+/K+-ATPase activity in H pyloripositive group (42 patients) was slightly higher than thatin H pylori-negative group (29 patients) (169.65±52.9 and eradication of H pylori, the gastric H+/K+-ATPase activities slightly decreased compared to prior therapy (165.03±59.50 The mean basal gastrin concentration was slightly higher in H pylori-positive patients than in H pylori-negative patients (87.92±39.65 pg/mL vs75.04± 42.57 pg/mL, P= 0.228). The gastrin levels fell significantly after the eradication of Hpylori. (Before treatment 87.00±30.78 pg/mL, aftertreatment 64.73±18.96 pg/mL, P = 0.015).CONCLUSION: Gastric H+/K+-ATPase activities are not associated with H pylori status in patients with chronicgastritis. | Duangporn Thong-Ngam Pisit Tangkijvanich Pichet Sampatanukul Paungpayom Prichakas Varocha Mahachai Piyaratana Tosukowong | 2005 | World Journal of Gastroenterology2005，11，23： | 3 |
| 12 | Role of serum total sialic acid in differentiating cholangiocarcinoma from hepatocellular carcinoma显示文摘AIM:This study was designed to evaluate the clinical application of serum total sialic acid (TSA) in the diagnosis of cholangiocarcinoma (CCA).METHODS: Serum TSA was determined by periodateresorcinol microassay in 69 patients with CCA, 59 patients with hepatocellular carcinoma (HCC), 37 patients with cirrhosis, 61 patients with chronic hepatitis and 50 healthy blood donors.RESULTS: The mean serum TSA concentration in CCA (2.41±0.70 mmol/L) was significantly higher than those of HCC, cirrhosis, chronic hepatitis and healthy blood donors (1.41±0.37 mmol/L, 1.13±0.31 mmol/L, 1.16±0.26 mmol/L, and 1.10±0.14 mmol/L, respectively; P<0.001). Based on ROC curve analysis, a cut-off point of 1.75 mmol/L discriminated between CCA and HCC with a sensitivity,specificity and accuracy of 82.6%, 83.1%, and 82.8%,respectively.CONCLUSION: Based on our results, serum TSA would be a useful marker for the differential diagnosis of CCA from HCC. | Prachya Kongtawelert Pisit Tangkijvanich Siriwan Ong-Chai Yong Poovorawan | 2003 | World Journal of Gastroenterology2003，9，10： | 3 |
| 13 | Acadaspora gerdenaii,and its sunanamorph Glomus lepratichum based upon 18S rDNA gene sequences显示文摘 | Hiromichi Sauako Koya augawara Masanoti salto phylogentic pisition of an arbuscular nyucorrizal fyngus | 1998 | Mycoscience1998，，39： | 1 |
| 14 | Paphiopedilum species of Thailand 显示文摘 | Pisit Voraurai | 1980 | Proceedings of the 9^th world orchid conferences1980，3941，： | 1 |
| 15 | Characterisation of osmotically dehydrated papaya with further hot air drying and microwave vacuum drying显示文摘 | Nutthanun Nimmanpipug Nantawan Therdthai Pisit Dhamvithee | 2013 | Int J Food Sci Technol2013，，6： | 1 |
| 16 | Molecular analysis of hepatitis B virus associated with vaccine failure in infants and mothers: A case–control study in Thailand显示文摘 | Pattaratida Sa‐nguanmoo Pisit Tangkijvanich Piyanit Tharmaphornpilas Aim‐orn Rasdjarmrearnsook Saowanee Plianpanich Nutchanart Thawornsuk Apiradee Theamboonlers Yong Poovorawan | 2012 | J Med Virol2012，，8： | 1 |
| 17 | A case–control study on sequence variations in the enhancer II/core promoter/precore and X genes of hepatitis B virus in patients with hepatocellular carcinoma显示文摘 | Pisit Tangkijvanich Pattaratida Sa-nguanmoo Varocha Mahachai Apiradee Theamboonlers Yong Poovorawan | 2010 | Hepatology International2010，，3： | 1 |
| 18 | Trimming a Metallic Biliary Stent Using an Argon Plasma Coagulator显示文摘 | Rungsun Rerknimitr Pisit Naprasert Pradermchai Kongkam Pinit Kullavanijaya | 2007 | CardioVascular and Interventional Radiology2007，，3： | 1 |
| 19 | Improving tuberculosis infection control practice and microbial air quality in a general hospital after intervention显示文摘Objective:Hospital personnel,especially nurses are at risk for tuberculosis(TB) infection and the intervention for reducing the risk should be established.Methods:To compare the TB infection control practices and standard precaution in 154 registered nurses and auxiliary nurses working in risk wards.Additionally,microbial air quality in the studied wards was investigated before and after implementation of an intervention including twoday training program on TB infection control and standard precaution practices and managing the ward environments. Results:Post-intervention,the percentage of studied nurses who always practiced increased in every item of TB infection control practice(6 items) and standard precaution(9 items) when compared with the preintervention period.Both mean scores were significantly higher than those before intervention(5.0±1.0 vs 4. 4±1.1,and 7.6±1.3 vs 6.7±1.5,P<0.001,respectively).As well as,bacterial and fungal counts in air samples collected from every studied ward decreased after implementing the intervention,for example, mean of bacterial counts in OPD decreased from 387.8±249.5 cfu/m^3 to 194.4±134.3 cfu/m^3.Additionally, there was no air sample with high level of bacterial and fungal counts,whereas,3.7 -22.2%of air samples collected before intervention had high level of bacterial counts(>500 cfu/m^3).Conclusion:Two-day training program and management of the ward environment could improve the scores of TB infection control practices and the standard precaution among studied nurses and reduce the microbial counts in air samples collected from the studied wards. | Pipat Luksamijarulkul Jiraporn Khumsri Pisit Vatthanasomboon Pornapa Aiumlaor | 2009 | Asian Pacific Journal of Tropical Medicine2009，2，2： | 1 |
| 20 | Characterization of microwave vacuum-dried durian chips 显示文摘 | Swittra B Nantawan T Pisit D | 2011 | Journal of Food Engineering2011，，104： | 1 |
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