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| 1 | TIP30 regulates apoptosis-related genes in its apoptotic signal transduction pathway显示文摘AIM: To investigate the role of TIP30 in apoptotic signal pathway in hepatoblastoma cells and to provide a basis for TIP30 as a gene therapy candidate in the regression of hepatoblastoma cells.METHODS: Apoptosis of human hepatoblastoma cell lines HepG2 (p53 wild), Hep3B (p53 null) and PLC/RPF/5 (p53mutant) infected with Ad-TIP30 (bearing a wild type human Tip30 gene) were analyzed and p53, Bax and Bclxl expression levels were compared among these cells.MlT assay, DNA fragmentation, in situ 3' end labeling of DNA, annexin-Ⅴ FITC staining were used to detect cell death and apoptosis in cells at various time intervals subsequent to infection, and to determine whether TIP30 had an effect on the expression levels of some apoptosis-related gene products such as Bax, p53 and Bcl-xl. A similar time course experiment was performed by Western blotting.RESULTS: In MTT assay, the viability of HepG2 cells decreased significantly from 99.7% to 10% and displayed more massive cell death within 5-8 d than Hep3B and PLC/RPF/5 cells, with their viability decreased from 97.8% to 44.3% and 98.1% to 50.4%, respectively. In annexin-ⅤFITC assay, the percentage of apoptosis cells in HepG2cells was two to three-fold higher than that in control cells (infected with Ad-GFP), two-fold higher than that in Hep3B cells and 1.4-fold higher than that in PLC/RPF/5 cells 36 h after infection, respectively. Moreover, in HepG2 cells, the p53 began to increase 6-8 h after infection, reaching a maximum level between 8 and 12 h after infection and then dropped. Bax showed a similar increase in the cells as p53 reached the maximum at 8-12 h and subsequently decreased. Interestingly, Bcl-xl protein levels were down regulated during 24 to 36 h after Ad-TIP30 infection. In contrast, ectopic expression of TIP30 in Hep3B and PLC/RPF/5 cells had no effect on the regulation of Bax expression, but had an effect on Bcl-xl levels. In comparison with HepG2 cells, these data suggested that up-regulation of p53 levels by TIP30 might be a pre-requisite for Bax and Bax/Bcl-xl ratio increase. We hypothesized that TIP30 might regulate Bax gene partly through p53, which sensitizes cells to apoptosis by involving a p53 apoptosis signal transduction pathway.CONCLUSION: TIP30 plays an important role in predisposing hepatoblastoma cells to apoptosis through regulating expression levels of these genes. Ad-TIP30carrying exogenous TIP30-anti-tumor genes may be regarded as a potential candidate for the treatment of hepatocellular carcinoma. | MeiShi XiaZhang PingWang Hong-WeiZhang Bai-HeZhang Meng-ChaoWu | 2005 | World Journal of Gastroenterology2005，11，2： | 22 |
| 2 | Crystal structure of PHD domain of UHRF1 and insights into recognition of unmodified histone H3 arginine residue 2显示文摘 | LuluHu ZeLi PingWang YanLin YanhuiXu | 2011 | Cell Research2011，21，9： | 7 |
| 3 | Detection of CK20mRNA in peripheral blood of pancreatic cancer and its clinical significance显示文摘AIM: To detect the expression of CK20mRNA in peripheral blood of Pancreatic cancer and evaluate its clinical significance.METHODS: Expression of CK20mRNA in peripheral blood was detected by fluorogenic qualitative reverse transcription-polymerase chain reaction (RT-PCR) in 40cases of pancreatic cancer at the night before operation,in 5 cases of benign pancreatic diseases, in 5 cases of healthy individuals. The relationships were investigated between CK20mRNA expression and the clinicopathological variables, and clinical follow-up outcome in those patients with pancreatic cancer having undergone radical resection.RESULTS: Of the 40 patients with pancreatic cancer, 23(57.5%) cases were positive for CK20mRNA expression.CK20mRNA expression was significantly correlated with lymphatic metastasis (P = 0.008), histopathological grading (P = 0.009), and pathological stage (P = 0.021);there was no significant correlation between CK20mRNA expression and age, gender, tumor diameter, and depth of invasion. The cumulative metastasis rates of patients with CK20mRNA expression were higher than those of patients with no CK20mRNA expression within 6 mo (34.7%vs5.9%, P= 0.043) or 12 mo (73.9% vs35.3%, P= 0.02)after operation. CK20mRNA expression in peripheral blood of pancreatic cancer indicated poorer prognosis. The survival rate of patients with CK20mRNA expression was lower than that of patients with negative CK20mRNA expression (Log-Rank = 13.31, P = 0.0003).CONCLUSION: CK20mRNA is a sensitive and specific molecular marker for the detection of micrometastasis in peripheral blood of patients with pancreatic cancer. The CK20mRNA expression in peripheral blood is correlated with biological characteristic of pancreatic cancer. It can help to predict the prognosis of pancreatic cancer after operation, and to determine which patient will benefit from aggressive adjuvant therapies. | Yun-LiZhang Jian-GuoFeng Jian-MinGou Li-XinZhou PingWang | 2005 | World Journal of Gastroenterology2005，11，7： | 2 |
| 4 | Diabetes mellitus and risk of hepatocellular carcinoma: a systematic review and meta‐analysis显示文摘 | PingWang DonghongKang WeiCao YanWang ZhiwenLiu | 2012 | Diabetes Metab Res Rev2012，，2： | 2 |
| 5 | Microstructures and mechanical properties of Mg–10Gd–6Y–2Zn–0.6Zr(wt.%) alloy显示文摘 | Liang Zheng Chuming Liu Yingchun Wan Pingwang Yang Xin Shu | 2011 | Journal of Alloys and Compounds2011，，35： | 2 |
| 6 | Mul tienzymatic synthesis of polyhydroquinone for use as a Redox Poly mer 显示文摘 | Brett D Martin Sangamitra Parida | 1995 | JAm ChemSoc1995，117，12： | 1 |
| 7 | Control PointAdjustment for B-spline Curve Approximation显示文摘 | Huai Pingyang Wen Pingwang Jia Guangsun | 2004 | Computer-aided Design2004，36，7： | 1 |
| 8 | Study on the interaction of 3,3-bis (4-hydroxy-l-naphthyl)-phthalide with bovine serum albumin by fluorescence spectroscopy显示文摘 | YA PINGWANG YAN IIWEI CHUAN DONG | 2006 | Journal of Photochemistry and Photobiology A: Chemistry2006，177，： | 1 |
| 9 | A spatial network approach to urban configurations显示文摘 | Fan‐chinKung PingWang | 2012 | Canadian Journal of Economics/Revue canadienne d’économique2012，，1： | 1 |
| 10 | Galectin‐1 attenuates astrogliosis‐associated injuries and improves recovery of rats following focal cerebral ischemia显示文摘 | Wen‐ShengQu Yi‐HuiWang Jun‐FangMa Dai‐ShiTian QiangZhang Deng‐JiPan Zhi‐YuanYu Min‐JieXie Jian‐PingWang WeiWang | 2010 | Journal of Neurochemistry2010，，2： | 1 |
| 11 | Ginsenoside Rg1 modulation on thrombospondin‐1 and vascular endothelial growth factor expression in early renal fibrogenesis in unilateral obstruction显示文摘 | Xi‐ShengXie Heng‐ChuanLiu Feng‐PingWang Cheng‐LongZhang ChuanZuo YaoDeng Jun‐MingFan | 2010 | Phytother Res2010，，11： | 1 |
| 12 | Desulfurization of dibenzothiophene to 2- hydroxybiphenyl by some newly isolated bacterial strains 显示文摘 | Steven Krawiec | 1994 | Arch Microbial1994，161，： | 1 |
| 13 | Circulating Toll‐like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C显示文摘 | JIU‐PINGWANG YEZHANG XINWEI JUNLI XUE‐PINGNAN HAI‐TAOYU YULI PING‐ZHONGWANG XUE‐FANBAI | 2010 | APMIS2010，，4： | 1 |
| 14 | Acquired hepatocerebral degeneration: A case report显示文摘AIM: Acquired hepatocerebral degeneration (AHD) is an exceptional type of hepatic encephalopathies (HE). It is characterized by neuropsychiatric and extrapyramidal symptomathology similar to that seen in hepatolenticular degeneration (Wilson's disease). In this paper, we report a case of AHD with unusual presenting features.METHODS: A 28-year-old man with AHD was described and the literature was reviewed.RESULTS: The man had a history of HBV-related liver cirrhosis. He was admitted to our hospital with apathy,dysarthria, mild consciousness impairment and extrapyramidal symptoms after hematemesis. By review of the literature,cases with AHD often did not present consciousness impairment. So our case was once diagnosed incorrectly as Wilson's disease.CONCLUSION: AHD is a rare syndrome and its variable clinical manifestations make it difficult to be diagnosed.But we believe that extensive examination and thorough understanding of the disease are beneficial to a correct diagnosis. Moreover, biocoene is effective in treating the case. | Wei-XingChen PingWang Sen-XiangYan You-MingLi Chao-HuiYu Ling-LingJiang | 2005 | World Journal of Gastroenterology2005，11，5： | 1 |
| 15 | Enantioselective enzymatic hydrolysis of racemic glycidyl butyrate by lipase from Bacillus subtilis with improved catalytic properties 显示文摘 | Chun - yuan Li PingWang Dan - tong Zhao | 2008 | Journal of Molecular Catalysis B: Enzymatic2008，55，34： | 1 |
| 16 | Efficient generation of functional hepatocyte‐like cells from human fetal hepatic progenitor cells in vitro显示文摘 | WeitaoZhang WeihongLi BaoqingLiu PingWang WenLi HaiyanZhang | 2012 | J Cell Physiol2012，，5： | 1 |
| 17 | Construction of mammary-specific vector of tissue-type plasminogen activator显示文摘 | QinxianZhang YiDing PingWang ShumanLiu XiaoyanZhu | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
| 18 | A NEW CELL CLONE DERIVED FROM TRICHOPLUSIA NI TN5B1-4 CELLS显示文摘The characteristics of a cultured cell line do not always remain stable and may change upon continuous passage. Most continuous cell lines, even after cloning, possess several genotypes that are constantly changing. There are numerous selective and adaptive culture processes, in addition to genetic instability, that may improve phenotypic change in cell growth, virus susceptibility, gene expression, and production of virus. Similar detrimental effects of long term passaging of insect cells have also been reported for continuous cell lines, for example, Tn5B 1-4 cells, which are the most widely used for the baculovirus expression vector system (BEVS), provide superior production of recombinant proteins,however, this high productivity may be more evident in low passage cells. In this paper, we describe the isolation of a cell clone, Tn5B-40, from low passage Tn5B 1-4 cells. The growth characteristics,productions of virus, and high level of recombinant protein productions were determined. The results showed the susceptibility of both clone and Tn5B 1-4 cells to wild-type AcNPV was approximately the same rate with over 95% of infection; when the cloned cells were infected with recombinant baculoviruses expressing β-galactosidase and secreted alkaline phosphatase (SEAP), expression of the recombinant proteins from the cloned cells exceeded that from the parental Tn5B 1-4 cells. | Jian-xiaoTian Chang-youLi Gui-lingZheng Guo-xunLi PingWang Granados | 2004 | Entomologia Sinica2004，11，3： | 0 |
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