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| 1 | Bacterial presence on flexible endoscopes vs time since disinfection显示文摘AIM To correlate the length of endoscope hang time and number of bacteria cultured prior to use.METHODS Prospectively, we cultured specimens from 19 gastroscopes, 24 colonoscopes and 5 side viewing duodenoscopes during the period of 2011 to 2015. A total of 164 results had complete data denoting date of cleansing, number of days stored and culture results. All scopes underwent initial cleaning in the endoscopy suite utilizing tap water, and then manually cleaned and flushed. High level disinfection was achieved with a Medivator~? DSD(Medivator Inc., United States) automated endoscope reprocessor following manufacturer instructions, with Glutacide~?(Pharmax Limited, Canada), a 2% glutaraldehyde solution. After disinfection, all scopes were stored in dust free, unfiltered commercial cabinets for up to 7 d. Prior to use, all scopes were sampled and plated on sheep blood agar for 48 h; the colony count was obtained from each plate. The length of endoscope hang time and bacterial load was analyzed utilizing unpaired t-tests. The overall percentage of positive and negative cultures for each type of endoscope was also calculated. RESULTS All culture results were within the acceptable range(less than 200 cfu/mL). One colonoscope cultured 80 cfu/mL after hanging for 1 d, which was the highest count. ERCP scopes cultured at most 10 cfu, this occurred after 2 and 7 d, and gastroscopes cultured 50 cfu/mL at most, at 1 d. Most cultures were negative for growth, irrespective of the length of hang time. Furthermore, all scopes, with the exception of one colonoscope which had two positive cultures(each of 10 cfu/mL), had at most one positive culture. There was no significant difference in the number of bacteria cultured after 1 d compared to 7 d when all scopes were combined(day 2: P = 0.515; day 3: P = identical; day 4: P = 0.071; day 5: P = 0.470; day 6: P = 0.584; day 7: P = 0.575). There was also no significant difference in the number of bacteria cultured after 1 day compared to 7 d for gastroscopes(day 2: P = 0.895; day 3: P = identical; day 4: P = identical; day 5: P = 0.893; day 6: P = identical; day 7: P = 0.756), colonoscopes(day 2: P = 0.489; day 4: P = 0.493; day 5: P = 0.324; day 6: P = 0.526; day 7: P = identical), or ERCP scopes(day 2: P = identical; day 7: P = 0.685). CONCLUSION There is no correlation between hang time and bacterial load. Endoscopes do not need to be reprocessed if reused within a period of 7 d. | Katlin I Mallette Peter Pieroni Sonny S Dhalla | 2018 | World Journal of Gastrointestinal Endoscopy2018，10，1： | 3 |
| 2 | Exhaled carbon monoxide in sarcoidosis显示文摘 | Ciarleglio G Refini RM Pieroni MG | 2008 | Sarcoidosis Vasc Diffuse Lung Dis2008，25，1： | 1 |
| 3 | Aluminium market and the macroeconomy显示文摘 | Melisso Boschi Luca Pieroni | 2008 | Journal of Policy Modeling2008，，2： | 1 |
| 4 | Biochemical markers of liver fibrosis in patients with hepatitis C virus infection : a prospective study 显示文摘 | Imbert - Bismut F Ratziu V Pieroni L | 2001 | Lancet2001，357，9262： | 1 |
| 5 | Smoothmusde cells in human atheroscle-rotic plaques secrete and proliferate in response to high mobility group box 1 protein显示文摘 | Porto A Palumbo R Pieroni M | 2006 | FASEB J2006，20，： | 1 |
| 6 | Smooth muscle cells in human atherosclerotic plaques secrete and proliferate in response to high mobility group box 1 protein 显示文摘 | Porto A Palumbo R Pieroni M | 2006 | FASEB2006，20，14： | 1 |
| 7 | Addiction, social interactions and gender differences in cigarette consumption显示文摘 | Aristei D Pieroni L | 2009 | Empirica2009，36，： | 1 |
| 8 | Optically Active Polymers of 1-Alkynes显示文摘 | CIARDELLI F LANZILLO S PIERONI O | 1974 | Macromolecules1974，7，： | 1 |
| 9 | Biochemical markers of liver fibrosis in patients with hepatitis C virus infection:a prospective study显示文摘 | Inbert-Bismut F Ratzia V Pieroni L | 2001 | Lancet2001，357，9262： | 1 |
| 10 | Myocardial production of chromogranin A in human heart:a new regulatory peptide of cardiac function显示文摘 | Pieroni M | 2007 | Eur Heart J2007，28，9： | 1 |
| 11 | Concealed cardiomyopathies in competitive athletes with ventricular arrhythmias and an apparently normal heart:role of cardiac electroanatomical mapping and biopsy显示文摘 | Dello Russo A Pieroni M Santangeli P | | 0，，12： | 1 |
| 12 | Fabry's disease cardiomyopathy:echocardiographic detection of endomyocardial glycosphingolipid compartmentalization显示文摘 | PIERONI M CHIMENTI C DE COBELLI F | 2006 | J Am Coll Cardiol2006，47，： | 1 |
| 13 | Myocarial production of chrmogranin A in human heart:a new regulatory peptide of cardiac function显示文摘 | Pieroni M Corti A Tota B | 2007 | Eur Heart J2007，28，9： | 1 |
| 14 | Medicinal plants and food medicines in the folk traditions of the upper Lucca Province, Italy显示文摘 | PIERONI A | 2000 | Journal of Ethnopharmacology2000，70，3： | 1 |
| 15 | Biochemical markers of liver fibrosis in patients with hepatitis C vires infection: a prospective study显示文摘 | Imbert-Bismut F Ratziu V Pieroni L et ol | 2001 | Lancet2001，357，9262： | 1 |
| 16 | Proteasome inhibitors therapeutic strategies for cancer显示文摘 | D'lessandro A Pieroni L Ronci M | 2009 | Recent Pat Anticancer Drug Discov2009，4，1： | 1 |
| 17 | Biochemical markers of liver fibrosis in patients with hepatitis C virus infection: a prospective study 显示文摘 | Imbert-Bismut F Ratziu V Pieroni L | 2001 | Lancet2001，357，9262： | 1 |
| 18 | Ricin RCA60 evidence of its phospholipase activity显示文摘 | Helmy M Lombard S Pieroni G | 1999 | Biochem Biophys Res Commun1999，258，： | 1 |
| 19 | Biochemical markers of liver fibrosis in patients with hepatitis C virus infection: a prospective study显示文摘 | Fran?oise Imbert-Bismut Vlad Ratziu Laurence Pieroni Frederic Charlotte Yves Benhamou Thierry Poynard | 2001 | The Lancet2001，，9262： | 1 |
| 20 | Smooth muscle ceils in human athero-sclerotic plaques secrete and imoliferate in response to high mobility group box l protein 显示文摘 | Porto A Palumbo R Pieroni M | | FASEB0，20，14： | 1 |
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