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| 1 | Morphological and biochemical responses of Oryza sativa L.(cultivar MR219) to ion beam irradiation显示文摘Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses(10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam(10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics. | Anna Pick Kiong LING Ying Chian UNG Sobri HUSSEIN Abdul Rahim HARUN Atsushi TANAKA Hase YOSHIHIRO | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，12： | 5 |
| 2 | A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation显示文摘骆驼毛的织物感觉迟钝的异种, Beyma,作为屏蔽标准用根生长抑制从一张他们 mutagenesis 人口在莲花 japonicus MG-20 被孤立到应用骆驼毛的织物。(名字 Beyma 从澳大利亚的土著的语言被拿, Wagiman, beyma,意思枯竭) 因为一个主导的孟德尔的变化对骆驼毛的织物感觉迟钝,分离的稳定的异种导致了萌芽,植物的生长,有气孔的洞,以及生节的抑制。织物骆驼毛的织物层次是正常的,建议敏感而非生合成变化。它是慢成长的(5070% 野类型的 MG-20 ) 并且在组成附近的 wilty 显型与它的无能联系了调整有气孔的开。虽然显示出大量骆驼毛的织物感觉迟钝的显型,, Beyma 没当增加的骆驼毛的织物,数字和 patterning 不在时显示出小瘤数字控制的改变(然而并非尺寸) 在异种形成的小瘤类似于 MG-20 的。MG-20 的裂口根实验证明根的一个方面上的骆驼毛的织物的申请局部地然而并非全身地禁止了生节。我们建议那件骆驼毛的织物不直接涉及生节(AON ) 的全身的自动调整。 | Bandana Biswas Pick Kuen Chan Peter M Gresshoff | 2009 | Molecular Plant2009，2，3： | 4 |
| 3 | Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata显示文摘Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs. | Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，7： | 2 |
| 4 | Classical Ethylene Insensitive Mutants of the Arabidopsis EIN2 Orthologue Lack the Expected‘hypernodulation’ Response in Lotus japonicus显示文摘Three independent ethylene insensitive mutants were selected from an EMS-mutagenized population of Lotus japonicus MG-20 (Miyakojima). The mutants, called 'Enigma', were mutated in the LjEIN2a gene from Lotus chromosome 1, sharing significant homology with Arabidopsis EIN2 (ethylene-insensitive2). All three alleles showed classical ethylene insensitivity phenotypes (e.g., Triple Response), but lacked the increased nodulation phenotype commonly associated with ethylene insensitivity. Indeed, all showed a marginal reduction in nodule number per plant, a phenotype that is enigmatic to sickle, an ethylene-insensitive EIN2 mutant in Medicago truncatula. In contrast to wild type, but similar to an ETR1-1 ethylene ethylene-insensitive transgenic of L. japonicus, enigma mutants formed nodules in between the protoxylem poles, demonstrating the influence of ethylene on radial positioning. Suppression of nodule numbers by nitrate and colonisation by mycorrhizal fungi in the enigma-1 mutant were indistinguishable from the wild type MG-20. However, reflecting endogenous ethylene feedback, the enigma-1 mutant released more than twice the wild-type amount of ethylene. enigma-1 had a moderate reduction in growth, greater root mass (and lateral root formation), delayed flowering and ripening, smaller pods and seeds. Expression analysis of ethylene-regulated genes, such as ETR1, NRL1 (neverripe-like 1), and EIL3 in shoots and roots of enigma-1 and MG-20 illustrated that the ethylene-insensitive mutation strongly affected transcriptional responses in the root. These mutants open the possibility that EIN2 in L. japonicus, a determinate nodulating legume, acts in a more complex fashion possibly through the presence of a duplicated copy of LjEIN2. | Pick Kuen Chan Bandana Biswas Peter M. Gresshoff | 2013 | Journal of Integrative Plant Biology2013，55，4： | 2 |
| 5 | Activation of a74kD plasma membrane protein kinase by hyperosmotic shocks in the halotolerant alga Dunaliela salina 显示文摘 | Chiflaru E Seger R Pick U | 1997 | Journal of Plant Physiology1997，151，： | 1 |
| 6 | Is type 2 diabetes mellitus a disease of the innate immune system显示文摘 | Pick JC Crook MA | 1998 | Diahetologia1998，41，： | 1 |
| 7 | Is type 2 diabetes mellitus a disease of the innate immune system显示文摘 | Pick JC CrookMA | 1998 | Diabetologia1998，41，： | 1 |
| 8 | Pleural effusion- presenting sign in multiple myehmm 显示文摘 | Shoenfeld Y Pick AI Weinberger A el al | 1978 | Respiralion1978，36，： | 1 |
| 9 | Suppression of rat adjuvant disease by cyclophosphamide pretreatment:evi- dence for an antibody mediated component in the pathogen esis of the disease显示文摘 | Mackenzie AR Pick CR Sibley PR | 1978 | Clin Exp Immnnol1978，32，4： | 1 |
| 10 | Pulmonary aspiration in a long-term care setting: clinical laboratory observations and an analysis of risk factors 显示文摘 | Pick N McDonald A Bennett N | 1996 | J Am Geriatr Soc1996，44，7： | 1 |
| 11 | From selective to highly selective SSRIs: a comparison of the antinociceptive properties of fluoxetine, fluvoxamine, citalopram and escitalopram显示文摘 | SCHREIBER S PICK CG | 2006 | Eur Neuropsychopharmacol2006，16，6： | 1 |
| 12 | Species-specific phytoplankton responses to nutrient enrichment in limnetic enclosures显示文摘 | Pick F R | 1989 | Archiv Fuer Hydrobiologie Ergebnisse der Limnologie1989，32，： | 1 |
| 13 | The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamate/glutamine levels and is important for growth in vivo显示文摘 | Cowley S Ko M Pick N Chow R Downing KJ Gordhan BG Betts JC Mizrahi V Smith DA Stokes RW Av-Gay Y | 2004 | Mol Microbiol2004，52，6： | 1 |
| 14 | High HSP90 expression is associated with decreased survival in breast cancer显示文摘 | Pick E Kluger Y Giltnane JM | 2007 | Cancer Res2007，67，7： | 1 |
| 15 | Mechanisms of cadmium mobility and accumulation in indian mustard显示文摘 | SALT D E PRINCE R C PICKING I J | 1995 | Plant Physiol1995，109，： | 1 |
| 16 | Selection and characterization of Dunaliella salina mutants defective in haloadaptation显示文摘 | Chitlaur E Pick U | 1989 | Plant Physiol1989，91，： | 1 |
| 17 | Differential reactivity of ? - carotene isomers from Dunaliella bardawil toward oxygen radicals显示文摘 | Jimenez C Pick C | 1993 | Plant physiol1993，101，： | 1 |
| 18 | Pilotstudy of operativefixation of fractured ribs in patients with flailchest显示文摘 | MarascoS Cooper J Pick A | 2009 | ANZ JSurg2009，79，11： | 1 |
| 19 | Determination of DEHP in blood products stored in plastic bags by HPLC显示文摘 | Gerlai J Pinter J Pick | 1987 | Chromatographia1987，24，1： | 1 |
| 20 | Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures显示文摘 | PICK U | 1981 | Arch Biochem Biophys1981，212，1： | 1 |
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