共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli显示文摘The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently underway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specifically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incubated on basic Murashige and Skoog(MS) gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid(NAA), 2,4-dichlorophenoxyacetic acid(2,4-D), and indole-3-butyric acid(IBA), were tested at various concentrations(0.55, 1.1 and 1.65 μM) using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight(DW) of 17.3 mg; however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine(BAP) or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA + 2.2μM BAP combination produced friable callus with the highest biomass(93.3mg DW). When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83 mg DW, respectively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA + 2.2 μM BAP hormone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture. | Shashita JAYARAMAN Nurul Hazwani DAUD Rasmina HALIS Rozi MOHAMED | 2014 | Journal of Forestry Research2014，25，3： | 4 |
| 2 | 不同扦插条件对奇岗芒插条生根的影响显示文摘以奇岗芒为材料,探究不同扦插部位、扦插时间、外源激素对奇岗芒茎段扦插的成活率及生根的影响。结果表明:下部茎段扦插的幼苗生根与成活状况优于中部茎段扦插的幼苗;不同扦插时间处理8月下旬至9月初扦插幼苗成活率为87.5%,株高为44.05 cm,生根数为7.05条/株,最长根长为26.03 cm,地上部鲜干重为1.673,0.561 g/株,地下部的鲜干重为0.372,0.135 g/株,均达到最大值,且扦插幼苗生长状况最佳。在0、25、50、75、100 mg/L 5个浓度GA_3处理下,随着浓度的增加,茎段扦插幼苗的株高、最长根长、生根数及地上下部鲜干重均呈现先降低后升高再降低的变化趋势,且均在75 mg/L GA_3处理时达到最大值;而IBA、NAA处理对茎段扦插的生根与成活有抑制作用,且NAA处理的抑制作用最强。 | 李慧 范希峰 滕珂 岳跃森 武菊英 | 2020 | 草原与草坪2020，40，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员