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| 1 | Development of a national VNIR soil-spectral library for soil classification and prediction of organic matter concentrations显示文摘Soil visible-near infrared diffuse reflectance spectroscopy(vis-NIR DRS)has become an important area of research in the fields of remote and proximal soil sensing.The technique is considered to be particularly useful for acquiring data for soil digital mapping,precision agriculture and soil survey.In this study,1581 soil samples were collected from 14 provinces in China,including Tibet,Xinjiang,Heilongjiang,and Hainan.The samples represent 16 soil groups of the Genetic Soil Classification of China.After air-drying and sieving,the diffuse reflectance spectra of the samples were measured under laboratory conditions in the range between 350 and 2500 nm using a portable vis-NIR spectrometer.All the soil spectra were smoothed using the Savitzky-Golay method with first derivatives before performing multivariate data analyses.The spectra were compressed using principal components analysis and the fuzzy k-means method was used to calculate the optimal soil spectral classification.The scores of the principal component analyses were classified into five clusters that describe the mineral and organic composition of the soils.The results on the classification of the spectra are comparable to the results of other similar research.Spectroscopic predictions of soil organic matter concentrations used a combination of the soil spectral classification with multivariate calibration using partial least squares regression(PLSR).This combination significantly improved the predictions of soil organic matter(R2=0.899;RPD=3.158)compared with using PLSR alone(R2=0.697;RPD=1.817). | SHI Zhou WANG QianLong PENG Jie JI WenJun LIU HuanJun LI Xi Raphael A VISCARRA ROSSEL | 2014 | Science China Earth Sciences2014，57，7： | 32 |
| 2 | A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy显示文摘anti-CTLA-4 抗体由阻止 B7-CTLA-4 相互作用接近否定发信号引起肿瘤拒绝,这被假定。在比临床上有效的 dosing 完成的血浆层次更加高的集中,令人惊讶地, anti-CTLA-4 抗体 Ipilimumab 不由 CTLA-4 也不 CTLA-4 绑定堵住任何一 B7 trans-endocytosis 到使不能调动或联系房间的 B7。因而, Ipilimumab 不从人性化的任何一个 CTLA4 基因在树枝状的房间(DC ) 上增加 B7 (Ctla4 h/h ) 或人的 CD34 + 茎重新组成房间的 NSG 老鼠。在 Ctla4 高效地表示人和老鼠 CTLA4 基因,绑在人的 anti-CTLA-4 抗体然而并非老鼠 CTLA-4 的 h/m 老鼠导致 Treg 弄空和 Fc 受体依赖者肿瘤拒绝。堵住的抗体 L3D10 比得上在引起肿瘤拒绝的非阻塞的 Ipilimumab。显著地,输在人类化期间堵住活动的 L3D10 子孙在导致 Treg 弄空和肿瘤拒绝仍然保持充分能干。有效地在淋巴的器官堵住 CD4 T 细胞激活和 de novo CD8 T 细胞 priming 的 Anti-B7 抗体否定地不影响 Ipilimumab 的 immunotherapeutic 效果。因此,临床上有效的 anti-CTLA-4 mAb 由独立于检查点封锁的机制引起肿瘤拒绝但是依赖于主人 Fc 受体。我们为 CTLA-4 检查点的一个重评价的数据电话封锁假设并且为安全、有效的 anti-CTLA-4 mAbs 的下一代提供新卓见。 | Xuexiang Du Fei Tang Mingyue Liu Juanjuan Su Yan Zhang Wei Wu Martin Devenport Christopher A Lazarski Peng Zhang Xu Wang Peiying Ye Changyu Wang Eugene Hwang Tinghui Zhu Ting Xu Pan Zheng Yang Liu | 2018 | Cell Research2018，28，4： | 25 |
| 3 | Review of Current Status and Research Approaches to Nitrogen Pollution in Farmlands显示文摘In this paper, the history, current status, and research approaches to nitrogen pollution were reviewed using systems analysis and deductions. The seriousness of N pollution world-wide was highlighted and recommendations were made to address the situation. A new hypothesis based on phytoremediation, which means the use of plants to directly or indirectly degrade or remove contaminats from soil and water, was proposed. | NIE Sheng-wei GAO Wang-sheng CHEN Yuan-quan SUI Peng A Egrinya Eneji | 2009 | Agricultural Sciences in China2009，8，7： | 23 |
| 4 | Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma显示文摘AIM:To investigate the biological role of alpha fetoprotein (AFP) and its clinical signif icance in carcinogenesis of hepatocellular carcinoma (HCC).METHODS:Clinical analysis of HCC patients and im-munohistochemical examination were conducted to evaluate the relationship between serum AFP level and patient mortality. Confocal microscopy,Western blotting, dimethylthiahzolyl-2,5-diphenyl-tetrazolium bromide,Cell Counting Kit-8 assays and flow cytometry were performed to explore the possible mechanism.RESULTS: Among the 160 HCC patients enrolled in this study,130 patients survived 2 years (81.25%),with a survival rate of 86.8% in AFP < 2 0 μg/L group,88.9% in AFP 20-250 μg/L group,and 69.6% in AFP > 250 μg/L group, demonstrating a higher mortality rate in HCC patients with higher AFP levels. Surgical treatment was benef icial only in patients with low AFP levels.The mortality rate of HCC patients with high AFP levels who were treated surgically was apparently higher than those treated with conservative management.The results of immunohistochemistry showed that AFP and AFP receptor were merely expressed in tissues of HCC patients with positive serum AFP.Consistently,in vitro analysis showed that AFP and AFPS were expressed in HepG2 but not in HLE cells. AFP showed a capability to promote cell growth,and this was more apparent in HepG2 cells,in which the proliferation was increased by 3.5 folds. Cell cycle analysis showed that the percent-age of HepG2 cells in S phase after exposure to AFP was modestly increased.CONCLUSION:HCC patients with higher AFP levels show a higher mortality rate,which appears to be attributable to the growth promoting properties of AFP. | Peng Li Shan-Shan Wang Hui Liu Ning Li Michael A McNutt Gang Li Hui-Guo Ding | 2011 | World Journal of Gastroenterology2011，17，41： | 23 |
| 5 | Biofeedback-guided pelvic floor exercise therapy for obstructive defecation: An effective alternative显示文摘AIM: To compare biofeedback-guided pelvic floor exercise therapy(BFT) with the use of oral polyethylene glycol(PEG) for the treatment of obstructive defecation.METHODS: A total of 88 subjects were assigned to treatment with either BFT(n = 44) or oral PEG(n = 44). Constipation symptoms(including difficult evacuation, hard stool, digitation necessity, incomplete emptying sensation, laxative dependence, perianal pain at defecation, and constipation satisfaction), Wexner Scores, and quality of life scores were assessed after 1, 3, and 6 mo.RESULTS: At the 6 mo follow-up, the symptoms of the BFT group patients showed significantly greater improvements compared with the PEG group regarding difficult evacuation, hard stools, digitation necessity, laxative dependence, perianal pain at defecation, constipation satisfaction, Wexner Constipation Score, and quality of life score(P < 0.05). The quality of life score of the BFT group at the final follow-up time(6 mo) was 80 ± 2.2. After a complete course of training, improvements in the clinical symptoms of the BFT group were markedly improved(P < 0.05), and the Wexner Constipation Scores were greatly decreased compared with the oral PEG group(P < 0.05).CONCLUSION: We concluded that manometric biofeedback-guided pelvic floor exercise training is superior to oral polyethylene glycol therapy for obstructive defecation. | Ma-Mu-Ti-Jiang A ba-bai-ke-re Ni-Re Wen Yun-Long Hu Liang Zhao Tuerhongjiang Tuxun Aierhati Husaiyin Yalikun Sailai Alimujiang Abulimiti Yun-Hai Wang Peng Yang | 2014 | World Journal of Gastroenterology2014，20，27： | 12 |
| 6 | Pharmacokinetics of the prototype and hydrolyzed carboxylic forms of ginkgolides A, B, and K administered as a ginkgo diterpene lactones meglumine injection in beagle dogs显示文摘Ginkgo diterpene lactones meglumine injection(GDLI)is a commercially available product used for neuroprotection.However,the pharmacokinetic properties of the prototypes and hydrolyzed carboxylic forms of the primary components in GDLI,i.e.,ginkgolide A(GA),ginkgolide B(GB),and ginkgolide K(GK),have never been fully evaluated in beagle dogs.In this work,a simple,sensitive,and reliable method based on ultra-fast liquid chromatography-tandem mass spectrometry(UFLC-MS/MS)was developed,and the prototypes and total amounts of GA,GB,and GK were determined in beagle dog plasma.The plasma concentrations of the hydrolyzed carboxylic forms were calculated by subtracting the prototype concentrations from the total lactone concentrations.For the first time,the pharmacokinetics of GA,GB,and GK were fully assessed in three forms,i.e.,the prototypes,the hydrolyzed carboxylic forms,and the total amounts,after intravenous administration of GDLI in beagle dogs.It was shown that ginkgolides primarily existed in the hydrolyzed form in plasma,and the ratio of hydrolysates to prototype forms of GA and GB decreased gradually to a homeostatic ratio.All of the three forms of the three ginkgolides showed linear exposure of AUC to the dosages.GA,GB,and GK showed a constant half-life approximately 2.7,3.4,and 1.2 h,respectively,which were consistent for the forms at three dose levels(0.3,1.0,and 3.0 mg·kg^(-1))and after a consecutive injection of GDLI for 7 days(1.0 mg·kg^(-1)). | WANG Shu-Yao A Ji-Ye FEI Fei GENG Jian-Liang PENG Ying OUYANG Bing-Chen WANG Pei JIN Xiao-Liang ZHAO Yu-Qing WANG Jian-Kun GENG Ting LI Yan-Jing HUA NG Wen-Zhe WANG Zhen-Zhong XIAO Wei WANG Guang-Ji | 2017 | Chinese Journal of Natural Medicines2017，15，10： | 9 |
| 7 | Trogocytosis of CD80 and CD86 by induced regulatory 7 cells显示文摘 | Peng Gu Julia Fang Gao Cheryl A D'Souza Aleksandra Kowalczyk Kuang-Yen Chou Li Zhang | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 8 | Protective role of PI3-kinase/Akt/eNOS signaling in mechanical stress through inhibition of p38 mitogenactivated protein kinase in mouse lung显示文摘 | Xin-qi PENG Mahendra DAMARLA Jarrett SKIRBALL Stephanie NONAS Xiao-ying WANG Eugenia J HAN Emile J HASAN Xuan CAO Adel BOUEIZ Rachel DAMICO Rubin M TUDER Alfred M SClUTO Dana R ANDERSON Joe GNGARCIA David A KASS Paul M HASSOUN Jun-tian ZHANG | 2010 | Acta Pharmacologica Sinica2010，31，2： | 6 |
| 9 | 血清netrin-1增加与缺血性脑卒中预后改善相关显示文摘先前的实验研究表明,血清netrin-1与缺血性脑卒中进展相关。进一步了解缺血性脑卒中患者中netrin-1的变化可能为缺血性脑卒中的预后评估提供新的思路。该研究旨在探讨血清netrin-1与缺血性脑卒中预后的关系。研究人员测定3346例中国急性缺血性脑卒中抗高血压试验(China antihypertensive trial in acute ischemic stroke,CATIS)中缺血性脑卒中患者基线血清netrin-1水平,所有患者均在脑卒中发病后3个月进行随访。主要观察指标是脑卒中发病后3个月内死亡和严重残疾(改良Rankin 量表评分≥3)。 | 刘莉 叶鹏 Guo D Zhu Z Zhong C Peng H Wang A Xu T Peng Y Xu T Chen CS Li Q Ju Z Geng D Chen J Zhang Y He J | 2019 | 中华高血压杂志2019，27，5： | 6 |
| 10 | Expression,purification and immunocharacteristics of recombination UreB protein of H.pylori显示文摘INTRODUCTIONHelicobacter pylori (H . pylori) is associated with the development of chronic gastritis ,peptic ulcer and gastric cancer and gastric MALT lymphoma[1-9],H .pylori has many antigens ,including urease ,heat shock protein and vacuolating cytotoxin and so on ,and urease is an important factor in the colinization of the gastric mucosa and suspected to cause damage to the gastric mucosa[10-14].At the same time ,urdase is also one of the important protective antigens . | Chao Wu~1 Quan Ming Zou~1 Hong Guo~2 Xiao Peng Yuan~1 Wei Jun Zhang~1 Dong Shui Lu~1 Xu Hu Mao~1 ~1Department of Clinical Microbiology,Third Military Medical University,Chongqing 400038,China ~2Department of Gastroenterology,Xinqiao Hospital,Third Military Medical University,Chongqing 40003?,ChinaDr.Chao Wu graduated from Third Military Medical University as a postgraduate in 2000,now a lecturer,specialized in diagnosis,prevention and therapy of Helicobacter pylori infection,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 5 |
| 11 | Consensus on the digestive endoscopic tunnel technique显示文摘With the digestive endoscopic tunnel technique(DETT), many diseases that previously would have been treated by surgery are now endoscopically curable by establishing a submucosal tunnel between the mucosa and muscularis propria(MP). Through the tunnel, endoscopic diagnosis or treatment is performed for lesions in the mucosa, in the MP, and even outside the gastrointestinal(GI) tract.At present, the tunnel technique application range covers the following:(1)Treatment of lesions originating from the mucosal layer, e.g., endoscopic submucosal tunnel dissection for oesophageal large or circular early-stage cancer or precancerosis;(2) treatment of lesions from the MP layer, per-oral endoscopic myotomy, submucosal tunnelling endoscopic resection, etc.; and(3) diagnosis and treatment of lesions outside the GI tract, such as resection of lymph nodes and benign tumour excision in the mediastinum or abdominal cavity. With the increasing number of DETTs performed worldwide, endoscopic tunnel therapeutics, which is based on DETT, has been gradually developed and optimized. However, there is not yet an expert consensus on DETT to regulate its indications, contraindications, surgical procedure, and postoperative treatment.The International DETT Alliance signed up this consensus to standardize the procedures of DETT. In this consensus, we describe the definition, mechanism,and significance of DETT, prevention of infection and concepts of DETTassociated complications, methods to establish a submucosal tunnel, and application of DETT for lesions in the mucosa, in the MP and outside the GI tract(indications and contraindications, procedures, pre-and postoperative treatments, effectiveness, complications and treatments, and a comparison between DETT and other operations). | Ning-Li Chai Hui-Kai Li En-Qiang Linghu Zhao-Shen Li Shu-Tian Zhang Yu Bao Wei-Gang Chen Philip WY Chiu Tong Dang Wei Gong Shu-Tang Han Jian-Yu Hao Shui-Xiang He Bing Hu1 Bing Hu2 Xiao-Jun Huang Yong-Hui Huang Zhen-Dong Jin Mouen A Khashab James Lau Peng Li Rui Li De-Liang Liu Hai-Feng Liu Jun Liu Xiao-Gang Liu Zhi-Guo Liu Ying-Cai Ma Gui-Yong Peng Long Rong Wei-Hong Sha Pateek Sharma Jian-Qiu Sheng Shui-Sheng Shi Dong Wan Seo Si-Yu Sun Gui-Qi Wang Wen Wang Qi Wu Hong Xu Mei-Dong Xu Ai-Ming Yang Fang Yao Hong-Gang Yu Ping-Hong Zhou Bin Zhang Xiao-Feng Zhang Ya-Qi Zhai | 2019 | World Journal of Gastroenterology2019，25，7： | 5 |
| 12 | Characterization and target identification of a DNA aptamer that labels pluripotent stem cells显示文摘 | Zhonggang Hou Susanne Meyer Nicholas E Propson jeff Nie Peng Jiang Ron Stewart James A Thomson | 2015 | Cell Research2015，25，3： | 5 |
| 13 | Ribosome Biogenesis Factor Bmsl-like Is Essential for Liver Development in Zebrafish显示文摘Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs.Among them is ribosome biogenesis factor Bmsl,which is highly conserved from yeast to human.In yeast,Bmsl initiates ribosome biogenesis through recruiting Rcll to pre-ribosomes.However,little is known about the biological function of Bmsl in vertebrates.Here we report that Bmsl plays an essential role in zebrafish liver development.We identified a zebrafish bms1l^(sq163) mutant which carries a T to A mutation in the gene bmsl-like(bms1l).This mutation results in L^(152) to Q^(152) substitution in a GTPase motif in Bmsll.Surprisingly,bmsll^(sq163) mutation confers hypoplasia specifically in the liver,exocrine pancreas and intestine after 3 days post-fertilization(dpf).Consistent with the bmsll^(sq163) mutant phenotypes,whole-mount in situ hybridization(WISH) on wild type embryos showed that bmsll transcripts are abundant in the entire digestive tract and its accessory organs.Immunostaining for phospho-Histone 3(P-H3) and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1l^(sq163) giving rise to an under-developed liver.Therefore,our findings demonstrate that Bmsll is necessary for zebrafish liver development. | Yong Wanga,Yue Luoa,Yunhan Hong(b,),Jinrong Peng(a,),Lijan Lo(a,b,) a College of Animal Sciences,Zhejiang University,Hangzhou 310058,China b Department of Biological Sciences,National University of Singapore,Science Drive 4,Singapore 117543,Singapore | 2012 | Journal of Genetics and Genomics2012，39，9： | 4 |
| 14 | Pediatric Wilson disease presenting as acute liver failure:Prognostic indices显示文摘BACKGROUND Acute liver failure(ALF)can be a primary presentation of Wilson disease(WD).Mortality rates are high in WD with ALF(WDALF).Predictions of mortality in WDALF vary by model and are sometimes contradictory,perhaps because few patients are studied or WD diagnoses are questionable.AIM To determine the outcomes among well-documented WDALF patients and assess mortality model performance in this cohort.METHODS We reviewed the medical records of our pediatric WDALF patients(n=41 over 6-years-old,single-center retrospective study)and compared seven prognostic models(King’s College Hospital Criteria,model for end-stage liver disease/pediatric end-stage liver disease scoring systems,Liver Injury Unit[LIU]using prothrombin time[PT]or international normalized ratio[INR],admission LIU using PT or INR,and Devarbhavi model)with one another.RESULTS Among the 41 Han Chinese patients with ALF,WD was established by demonstrating ATP7B variants in 36.In 5 others,Kayser-Fleischer rings and Coombs-negative hemolytic anemia permitted diagnosis.Three died during hospitalization and three underwent liver transplantation(LT)within 1 mo of presentation and survived(7.3%each);35(85.4%)survived without LT when given enteral D-penicillamine and zinc-salt therapy with or without urgent plasmapheresis.Parameters significantly correlated with mortality included encephalopathy,coagulopathy,and gamma-glutamyl transpeptidase activity,bilirubin,ammonia,and serum sodium levels.Area under the receiver operating curves varied among seven prognostic models from 0.981 to 0.748 with positive predictive values from 0.214 to 0.429.CONCLUSION WDALF children can survive and recover without LT when given D-penicillamine and Zn with or without plasmapheresis,even after enlisting for LT. | Wei-Yuan Fang Kuerbanjiang Abuduxikuer Peng Shi Yi-Ling Qiu Jing Zhao Yu-Chuan Li Xue-Yuan Zhang Neng-Li Wang Xin-Bao Xie Yi Lu A S Knisely Jian-She Wang | 2021 | World Journal of Clinical Cases2021，9，14： | 4 |
| 15 | Emergence,Mating and Oviposition Behavior of the Chinese Population in Pink Bollworm,Pectinophora gossypiella (Lepidoptera:Gelechiidae)显示文摘The pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae) is one of the most serious lepidopteran pests of cotton in the world. This pest invaded China at the onset of the 20th century, possibly through repeated introductions from several different locations worldwide. In this paper, we describe different behavioral parameters of this Hubei P. gossypiella strain under laboratory and field conditions. Using an infra-red video recorder, we observed (nocturnal) emergence, flight and mating activities, and oviposition patterns. Moth emergence started from 13:00 and continued up till 23:00. Under laboratory and field conditions, 2-7-d-old moths initiated flight around sunset, peaked 50 min later and gradually declined until 04:00. Although mating started immediately after darkness (i.e., 20:00), mating behavior was most intense from 23:00 to 03:40. Oviposition also showed distinct time-related patterns, with approx. 70% eggs laid between 20:00 and 22:00. In the studies of the relationship between flight and oviposition, the duration of flight had an effect on oviposition. The 1-d-old moths flown for 6 and 12 h began oviposition earlier than the unflown ones, and the 6-h flight had no effect on the egg production. However, the longevity of the flown moths was shortened after flight. In addition, the peaks of oviposition for the flown moths were advanced 2-3 d. The age when the moths flied affected the oviposition of adult moths. The earlier the moths started to fly after emergence, the greater the fecundity they had. The average egg production of moths flown for 24 h was less than that of non-flown moths which were of the same age. Both flight and delay in mating could advance the oviposition peak. Our study provides detailed insights in nocturnal activities and the reproductive biology of local P. gossypiella populations, which could be employed to fine-tune current pest management programs in China. | WU Huai-heng HUANG Min-song WAN Peng Kris A G Wyckhuys WU Kong-ming | 2013 | Journal of Integrative Agriculture2013，12，4： | 3 |
| 16 | IMPACT OF DEBRIS FLOW ON RIVER CHANNEL IN THE UPPERREACHES OF THE YANGTZE RIVER显示文摘A debris flow gully is a 'sediment highway'. Debris flow carries huge amount of sediment into river in extreme short time. which reduces discharge capacity of the river and even blocks up the river and also causes river bank erosion. | Peng CUI (1 This research is a part of key project supported by National Natural Scence Foundation (basic) (project No. 49831010). 2 Institute of Mountain hazards and Environment, Dongchuan Debris Flow Study Station. Chinese Academy of Sciences. Chengdu | 1999 | International Journal of Sediment Research1999，14，2： | 3 |
| 17 | Synthesis, Crystal Structure and Calculated β Value of a Tetrahedral Zinc (II) Complex——Zn(2-NH_2py)_2Br_2显示文摘A new zinc(II) complex, Zn(2-NH2py)2Br2 (py=pyridine), has been synthesized, and its molecular structure has been confirmed by IR, elemental analysis and -ray crystal structure analysis. Its space group is P21/n with Mr=413.43 (C10H12Br2N4Zn), a=7.435(2), b=12.865(3), c=14.186(4)? b=94.08(2)? V=1353.5(5) 3, Z=4, Dc=2.029 g/cm3, F(000)=328, ?2.378mm-1, R=0.0283, wR=0.0501. The total observed reflections with I≥2?I) were 5314, of which the independent reflections were 3106. The complex structure contains a distorted tetrahedron formed by four atoms coordinated to zinc atom, namely two bromide atoms and two nitrogen atoms. The two ZnBr bond lengths within one molecule, 2.3763(6) and 2.4002(5)? respectively, are not equal; and so are the two ZnN bond lengths, which are 2.031(2) and 2.044(2)?respectively. The calculation results using PM3 method through MOPAC software package in Chem 3D show that its first molecular hyperpolarizability b value is 5.210-30esu, which is comparable with that of p-nitroaniline. No bulk SHG effect has been detected due to the centrosymmetric space group. | REN Peng a SU Nan-Bing a QIN Jin-Gui a Michael W. Day b CHEN Chuang-Tianc ( aDepartment of Chemistry, Wuhan University, Wuhan 43007, China) ( bBeckman Institute, California Institute of Technology, Pasadena, CA91125, USAQ ) ( cBeijing R & D Center | 2002 | Chinese Journal of Structural Chemistry2002，21，1： | 3 |
| 18 | Quantification of left ventricular mechanics using vector-velocity imaging, a novel feature tracking algorithm, applied to echocardiography and cardiac magnetic resonance imaging显示文摘 | LI Peng MENG Hong LIU Shi-zhen Mani A Vannan | 2012 | Chinese Medical Journal2012，，15： | 3 |
| 19 | Ly49 receptors activate angiogenic mouse DBA+ uterine natural killer cells显示文摘 | Patricia DA Lima Megan M Tu Mir Munir A Rahim Annie R Peng B Anne Croy Andrew P Makrigiannis | 2014 | Cellular & Molecular Immunology2014，11，5： | 3 |
| 20 | A core-shell structured COYID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity显示文摘Although inoculation of COVID-19 vaccines has rolled out globally,there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries.Here,we report on the development of a highly efficacious mRNA vaccine,SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex(LPP)nanoparticles.SWOT 23 is easy to produce using a large-scale microfluidics-based apparatus.The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability,and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration.Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123,represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2,but also a panel of variants including D614G and N501Y variants.In addition,SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge.Taken together,SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans. | Ren Yang Yao Deng Baoying Huang Lei Huang Ang Lin Yuhua Li Wenling Wang Jingjing Liu Shuaiyao Lu Zhenzhen Zhan Yufei Wang Ruhan A Wen Wang Peihua Niu Li Zhao Shiqiang Li Xiaopin Ma Luyao Zhang Yujian Zhang Weiguo Yao Xingjie Liang Jincun Zhao Zhongmin Liu Xiaozhong Peng Hangwen Li Wenjie Tan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
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