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| 1 | Phase and amplitude sensitivities in surface plasmon resonance hio and chemical sensing显示文摘 | KABASHIN A V PATSKOVSKY S GRIGORENKO A N | 2009 | Opt Express2009，17，21： | 1 |
| 2 | Decoy Strategies: The Structure of TL1A:DcR3 Complex显示文摘 | Chenyang Zhan Yury Patskovsky Qingrong Yan Zhenhong Li Udupi Ramagopal Huiyong Cheng Michael Brenowitz Xiao Hui Stanley G. Nathenson Steven C. Almo | 2011 | Structure2011，，2： | 1 |
| 3 | Biochemical and structural characterization of the human TL1A ectodomain 显示文摘 | Zhan C Yah Q Patskovsky Y | 2009 | Biochemistry2009，48，32： | 1 |
| 4 | Correlation between photoluminescence properties and morphology of laser-ablated Si/SiOx nanostructured films 显示文摘 | KABASHIN A V SYLVESTRE J P PATSKOVSKY S | 2000 | J Appl Phys2000，91，5： | 1 |
| 5 | Decoy strategies : the structure of TL1 A : DcR3 complex 显示文摘 | Zhan C Patskovsky Y Yah Q | 2011 | Structure2011，19，2： | 1 |
| 6 | In- tensity based surface plasmon resonance sensor u- sing a nano hole rectangular array显示文摘 | Blanchard-Dionne A-P Guyot L Patskovsky S | 2011 | Optics Ex- press2011，19，15： | 1 |
| 7 | Decoy Strategies: The Structure of TL1A:DcR3 Complex显示文摘 | Chenyang Zhan Yury Patskovsky Qingrong Yan Zhenhong Li Udupi Ramagopal Huiyong Cheng Michael Brenowitz Xiao Hui Stanley G. Nathenson Steven C. Almo | 2011 | Structure2011，，2： | 1 |
| 8 | Distinctive st-ructure of the human GSTM3 gene-inverted orientation relative to the muclass glutathione transferase gene cluster显示文摘 | Huang MQ Takayama T | 1999 | Arch Biochem Biophys1999，361，1： | 1 |
| 9 | Phase and amplitude sensitivities in surface plasmon resonance bio and chemical sensing 显示文摘 | Kabashin A V Patskovsky S P Grigorenko A N | 2009 | Opt Express2009，17，21： | 1 |
| 10 | 显示文摘 | PATSKOVSKY Y PATSKOVSKA L ALMO S C | 2006 | Biochemistry2006，45，12： | 1 |
| 11 | Novel homeo- tic CMS patterns generated in Nicotiana via cybridization with Hyoscyamus and Scopolia 显示文摘 | Zubko M K Zubko E Patskovsky Y | 1996 | J Exp Bot1996，47，8： | 1 |
| 12 | Phasesensitive spatially-modulated surface plasmon resonance polarimetry for detection of biomofecular inter- actions 显示文摘 | Patskovsky S Jacctuemart R Meunier M | 2008 | Sensors and Actuators B2008，133，2： | 1 |
| 13 | Functions of His107 in the catalytic mechanism of human glutathione S-transferase hGSTM1a-1a 显示文摘 | Patskovsky YV Patskovska LN Listowsky I | 1999 | Biochemistry1999，38，4： | 1 |
| 14 | Functions of His107 in the catalytic mechanism of human glutathione S-transferase hGSTM1a-1a显示文摘 | PATSKOVSKY Y V PATSKOVSKA L N LISTOWSKY L | 1999 | Biochemistry1999，38，： | 1 |
| 15 | Phase-sensitive time-modulated surface plasmon resonancepolarimetry for wide dynamic range biosensor 显示文摘 | Patskovsky S? Masonneuve M Meunicr M | 2007 | OpticsExpress2007，15，4： | 1 |
| 16 | 查看详情显示文摘 | Patskovsky S Kabashin A V Meunier M Luong J H T | | 0，，： | 1 |
| 17 | 查看详情显示文摘 | Patskovsky S Kabashin A V Meunier M | | 0，，： | 1 |
| 18 | Near-infrared surface plasmon resonance sensing on a silicon platform显示文摘 | Patskovsky Sergiy Kabashin Andrei V Meunier Michel | 2004 | Sensors and Actuators B:Chemical2004，97，23： | 1 |
| 19 | Distinctive struc- ture of the human GSTM3 gene-inverted orientation relative to the mu class glutathione transferase gene cluster 显示文摘 | Patskovsky YV Huang MQ Takayama T | 1999 | Arch Biochem Biophys1999，361，： | 1 |
| 20 | AB034. In vivo laser-mediated retinal ganglion cell optoporation using Kv1.1 conjugated gold nanoparticles显示文摘Background:There is a current void in efficient,cell-specific,retinal drug delivery systems,thus developing a safe,effective,selective drug delivery system would open novel therapeutic avenues.We previously demonstrated that femtosecond(fs)laser irradiation can transfect DNA plasmids into cultured cells in the presence of gold nanoparticles(AuNPs).These AuNPs locally amplify laser energy at a submicron range creating transient pores allowing exogenous genetic material or cell impermeable dyes to enter the cell.Here,we sought out to selectively optoporate retinal cells in vivo with functionalized AuNPs and a 800 nm femtosecond(fs)laser.Methods:The cell-surface Kv1.1 voltage-gated channel was chosen to selectively target retinal ganglion cells(RGC)in the rat retina.Citrate-capped spherical 100 nm AuNPs functionalized with orthopyridyl-disulfide-poly(ethylene glycol)(5 kDa)-N-hydroxysuccinimide(OPSS-PEG-NHS)conjugated to a Kv1.1 monoclonal antibody were injected intravitreally in Sprague Dawley rats 3 hours prior to irradiation,concomitantly to a FITC-dextran dye to detect optoporation.The eyes of anesthetized rats were placed in the beam path of a laser system consisting of an 800 nm,100 fs laser and a Heidelberg Spectralis HRA ophthalmoscope for fundus visualization.The rat retina was irradiated at powers ranging from 20-750 mW,the eyes fixed in 4%paraformaldehyde,dissected,rinsed,mounted and imaged by confocal microscopy.Results:Our novel laser system coupled to a Heidelberg ophthalmoscope allowed for a clear visualisation of the rat ocular fundus.A timecourse of AuNP intravitreal injections revealed that optimal nanoparticle dispersion on the retinal surface occurred at 3 hours post injection.Following Kv1.1-AuNP and FITC-dextran intravitreal injection and incubation,irradiation at 120-750 mW resulted in FITC uptake by retinal cells.Conclusions:Since living biological tissues absorb energy very weakly at 800 nm,this non-invasive tool may provide a safe,cost effective clinically relevant approach to selectively target retinal cells and limit complications associated with surgical interventions,and potential biological hazards associated with viral-based gene therapy. | Ariel M.Wilson Javier Mazzaferri Éric Bergeron Sergiy Patskovsky Santiago Costantino Michel Meunier Przemyslaw Sapieha | 2018 | Annals of Eye Science2018，，1： | 0 |
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