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| 1 | Regulatory mechanisms for abnormal expression of the human breast cancer specific gene 1 in breast cancer cells显示文摘Breast cancer-specific gene 1 (BCSG1), also referred as synuclein γ, was originally iso-lated from a human breast cancer cDNA library and the protein is mainly localized to presynaptic terminals in the nervous system. BCSG1 is not expressed in normal or benign breast lesions, but expressed at an extremely high level in the vast majority of the advanced staged breast carcinomas and ovarian carcinomas. Overexpression of BCSG1 in cancer cells led to significant increase in cell proliferation, motility and invasiveness, and metastasis. To elucidate the molecular mechanism and regulation for abnormal transcription of BCSG1, a variety of BCSG1 promoter luciferase reporters were constructed including 3′ end deleted sequences, Sp1 deleted, and activator protein-1 (AP1) domains mutated. Transient transfection assay was used to detect the transcriptional activation of BCSG1 promoters. Results showed that the Sp1 sequence in 5′-flanking region was involved in the basal transcriptional activities of BCSG1 without cell-type specificity. In comparison to pGL3-1249, the reporter activities of pGL3-1553 in BCSG1-negative MCF-7 cells and pGL3-1759 in HepG2 cells were notably decreased. Mutations at AP1 sites in BCSG1 intron 1 significantly reduced the promoter ac-tivity in all cell lines. Transcription factors, c-jun, c-fos and cyclin AMP-responsive element binding (CREB) protein, could markedly enhance the promoter activities. Thus, our results suggest that the abnormal expression of BCSG1 in breast cancer cells is likely regulated by multiple mechanisms. The 5′ flanking region of BCSG1 provides the basal transcriptional activity without cell type specificity. A critical promoter element involved in abnormal expression of BCSG1 presents in the first exon. The cell type specificity of BCSG1 transcription is probably affected through intronic cis-regulatory se-quences. AP1 domains in the first intron play an important role in control of BCSG1 transcription. | LU Aiping1, LI Qing1, LIU Jingwen2 1. Department of Pathology, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China 2. VA Palo alto Health Care System, Palo Alto, California, CA 94304, USA | 2006 | Science China(Life Sciences)2006，49，4： | 8 |
| 2 | Observation and modelling of snow and sea ice mass balance and its sensitivity to atmospheric forcing during spring and summer 2007 in the Central Arctic显示文摘Snow depth and sea ice thickness were observed applying an ice mass balance buoy(IMB)in the drifting ice station Tara during the International Polar Year in 2007.Detailed in situ observations on meteorological variables and surface fluxes were taken during May to August.For this study,the operational analyses and short-term forecasts from two numerical weather prediction(NWP)models(ECMWF and HIRLAM)were extracted for the Tara drift trajectory.We compared the IMB,meteorological and surface flux observations against the NWP products,also applying a one-dimensional thermodynamic sea ice model(HIGHTSI)to calculate the snow and ice mass balance and its sensitivity to atmospheric forcing.The modelled snow depth time series,controlled by NWP-based precipitation,was in line with the observed one.HIGHTSI reproduced well the snowmelt onset,the progress of the melt,and the first date of snow-free conditions.HIGHTSI performed well also in the late August freezing season.Challenges remain to model the“false bottom”observed during the melting season.The evolution of the vertical temperature profiles in snow and ice was better simulated when the model was forced by in situ observations instead of NWP results.During the melting period,the nonlinear ice temperature profile was successfully modelled with both forcing options.During spring and the melting season,total sea ice mass balance was most sensitive to uncertainties in NWP results for the downward longwave radiation,followed by the downward shortwave radiation,air temperature,and wind speed. | Bin CHENG Timo VIHMA Timo PALO Marcel NICOLAUS Sebastian GERLAND Laura RONTU Jari HAAPALA Donald PEROVICH | 2021 | Advances in Polar Science2021，32，4： | 4 |
| 3 | 液相色谱-紫外-核磁共振-质谱:多信息分离方法(英文)显示文摘The availability of liquid chromatography ultraviolet nuclear magnetic resonance mass spectrometry (LC UV NMR MS)integrated systems is providing greater information content and confidence for a variety of sample types, such asdrug metabolites, natural products, impurities, and degradants. A wide range of experimental strategies can be pur sued,highlighting the flexibility and performance possible with this multihyphenated technique. | Iain Green (Varian, Inc., 3120 Hansen Way, Palo Alto, CA 94303, U.S.A.) | 2003 | 波谱学杂志2003，20，4： | 3 |
| 4 | Weaning and noninvasive ventilation显示文摘 | Palo N | 2003 | Am J Respir Crit Care Med2003，168，： | 1 |
| 5 | Testing for non-Gaussianity of the cosmic microwave background in harmonic space: An empiri- cal process approach显示文摘 | Hanson F K Marinucci D Palo N | 2002 | Physical Review D2002，66，06： | 1 |
| 6 | A tutorial on decomposition methodsfor network utility maximization显示文摘 | PALO MAR D CHIANG M | 2006 | IEEE Journal on Select on Ar-ea in Communication2006，24，8： | 1 |
| 7 | Sentinel node mapping during laparoscopic distal gastrectomy for gastric cancer 显示文摘 | Orsenigo E Tomajer V Di Palo S | 2008 | Surg Endosc2008，22，1： | 1 |
| 8 | Dementia among medical inpatients显示文摘 | Erkinjuntti T Wikstrom J Palo J | 1986 | Arch Intern Med1986，146，10： | 1 |
| 9 | Ac- curacy of the clinical diagnosis of vascular dementia: a prospective clinical and post- mortem neuropathological study显示文摘 | Erkinjuntti T Haltia M Palo J | 1988 | J Neu- rol Neurosurg Psychiatry1988，51，： | 1 |
| 10 | Laparoscopic vs. Open Colectomies in Octogenarians: A Case-Matched Control Study显示文摘 | Andrea Vignali M.D. Saverio Palo M.D. Andrea Tamburini M.D. Giovanni Radaelli Ph.D. Elena Orsenigo M.D. Carlo Staudacher M.D | 2005 | Diseases of the Colon & Rectum2005，，11： | 1 |
| 11 | Laparoscopy-assisted gastrectomy versus open gastrectomy for gastric cancer:a monoinstitutional Western center experience显示文摘 | Orsenigo E Di Palo S Tamburini A et ai | 2011 | Surg Endosc2011，25，1： | 1 |
| 12 | Sources and photodecomposition of formaldehyde and acetaldehyde in Rome ambient air 显示文摘 | Possanzini M Di Palo V Cecinato A | 2002 | Atmos Environ2002，36，19： | 1 |
| 13 | Total mesorectal excision (TME) with laparoscopic approach:226 consecutive cases显示文摘 | Staudacher C Di Palo S Tamburini A | 2007 | Surg Oncol2007，16，1： | 1 |
| 14 | Measurements of lower carbonyls in Rome ambient air 显示文摘 | Possanzini M Palo V Petricea M | 1996 | Atmos Environ1996，30，： | 1 |
| 15 | Methanol steam refor ming for hydrogen production显示文摘 | Palo D R Dagle R A Holladay J D | 2007 | Chem Rev2007，107，10： | 1 |
| 16 | International terminology of colposcopy:an updated report from the International Federation for Cervical Pathology and Colposcopy显示文摘 | Walker P Dexeus S De Palo G | 2003 | Obstet Gynecol2003，101，： | 1 |
| 17 | Wnt signaling induces GLT-1 expression in rat C6 glioma cells显示文摘 | Palos TP Zheng S Howard BD | 1999 | J Neurochem1999，73，： | 1 |
| 18 | Developing microsatellite maekers for insect population structure-compex variation in a checkerspot butterfly显示文摘 | PALO J VARVIO S L HANDKI I | 1995 | Hereditas1995，123，： | 1 |
| 19 | Effect of weight loss and lifestyle changes on vascular inflammatory markers in obese women:a randomized trial显示文摘 | ESPOSITO K PONTILLO A DI PALO C | | 0，，14： | 1 |
| 20 | International terminology of colposcopy: an updated report from the International Federation for Cervical Pathology and Colposcopy显示文摘 | Walker P Dexeus S De Palo G | 2003 | Obstet Gynecol2003，101，1： | 1 |
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