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| 1 | Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice显示文摘BACKGROUND Non-alcoholic fatty liver disease (NAFLD) has become an epidemic largely due to the worldwide increase in obesity. While lifestyle modifications and pharmacotherapies have been used to alleviate NAFLD, successful treatment options are limited. One of the main barriers to finding safe and effective drugs for long-term use in NAFLD is the fast initiation and progression of disease in the available preclinical models. Therefore, we are in need of preclinical models that (1) mimic the human manifestation of NAFLD and (2) have a longer progression time to allow for the design of superior treatments. AIM To characterize a model of prolonged high-fat diet (HFD) feeding for investigation of the long-term progression of NAFLD. METHODS In this study, we utilized prolonged HFD feeding to examine NAFLD features in C57BL/6 male mice. We fed mice with a HFD (60% fat, 20% protein, and 20% carbohydrate) for 80 wk to promote obesity (Old-HFD group, n = 18). A low-fat diet (LFD)(14% fat, 32% protein, and 54% carbohydrate) was administered for the same duration to age-matched mice (Old-LFD group, n = 15). An additional group of mice was maintained on the LFD (Young-LFD, n = 20) for a shorter duration (6 wk) to distinguish between age-dependent and age-independent effects. Liver, colon, adipose tissue, and feces were collected for histological and molecular assessments.RESULTS Prolonged HFD feeding led to obesity and insulin resistance. Histological analysis in the liver of HFD mice demonstrated steatosis, cell injury, portal and lobular inflammation and fibrosis. In addition, molecular analysis for markers of endoplasmic reticulum stress established that the liver tissue of HFD mice have increased phosphorylated Jnk and CHOP. Lastly, we evaluated the gut microbial composition of Old-LFD and Old-HFD. We observed that prolonged HFD feeding in mice increased the relative abundance of the Firmicutes phylum. At the genus level, we observed a significant increase in the abundance of Adercreutzia, Coprococcus, Dorea, and Ruminococcus and decreased relative abundance of Turicibacter and Anaeroplasma in HFD mice. CONCLUSION Overall, these data suggest that chronic HFD consumption in mice can mimic pathophysiological and some microbial events observed in NAFLD patients. | Kandy T Velázquez Reilly T Enos Jackie E Bader Alexander T Sougiannis Meredith S Carson Ioulia Chatzistamou James A Carson Prakash S Nagarkatti Mitzi Nagarkatti E Angela Murphy | 2019 | World Journal of Hepatology2019，11，8： | 6 |
| 2 | Prostate-associated gene 4 (PAGE4), an intrinsically disordered cancer/testis antigen, is a novel therapeutic target for prostate cancer显示文摘联系前列腺的基因(PAGE4 ) 4 是是的显著地前列腺特定的癌症 / 睾丸抗原高度,在人的胎儿的前列腺和它的 diseased 的 upregulated 说然而并非在成年的正常的腺。PAGE4 是作为压力反应蛋白质工作压制反应的氧种类以及阻止 DNA 损坏的内在地混乱的蛋白质(IDP ) 。另外, PAGE4 也是加强的一个 transcriptional 管理者由 oncogene c-Jun.c6 月的 transactivation 与 Fos 家庭的成员一起由 heterodimerizing 形成 AP-1 建筑群并且在前列腺的发展和病理起一个重要作用,强调 PAGE4/c-Jun 相互作用的重要性。HIPK1,另外压力反应小径的一个部件,在为它的 transcriptional 活动批评的 T51 的 phosphorylates PAGE4。Phosphorylation 导致 conformational 并且在导致新细胞的功能的 PAGE4 整体的动态切换。最后,生物信息学证据建议 PAGE4 mRNA 能或者被拼接导致与潜伏的功能提到 conformational 整体的一个范围的可能性的多肽的四潜在的 isoforms。一起考虑了,数据建议 PAGE4 可以代表在应力和前列腺癌症(PCa ) 之间的第一个分子的连接。因此, pharmacologically 指向 PAGE4 可以是为与 PCa 对待并且设法病人的一个新奇机会,特别有低风险的疾病的病人。 | Prakash Kulkarni A Keith Dunker Keith Weninge John Orban | 2016 | Asian Journal of Andrology2016，18，5： | 3 |
| 3 | Application of homogeneous and heterogeneous cation-exchange membranes in coagulant recovery from water treatment plant residuals using Donnan membrane process显示文摘 | PRAKASH P HOSKINS D SENGUPTA A K | 2004 | Journal of Membrane Science2004，237，12： | 1 |
| 4 | Crista termindis atrial septal and coronary sinus activation during single and dual site atrial pacing显示文摘 | Prakash A Krol RB | 1996 | PACE1996，19，： | 1 |
| 5 | Scaled- up production of zero - trans margarine fat using pine nut oil and palm stearin显示文摘 | PRAKASH A ZHU Xuemei ANUPAMA G | 2010 | Food Chemistry2010，119，： | 1 |
| 6 | Synthesis of SAPO-34:high silicon incorporation in the presence of morpholine as tem- plate显示文摘 | Prakash A M Unnikrishnan S | 1994 | Journal of the Chemical Society Faraday Transac- tions1994，，90： | 1 |
| 7 | Long-term outcome of patients with drug-refractory atrial flutter and fibrillation after single- and dual- site atrial pacing for arrhythmia prevention显示文摘 | Delfaut P Saksena S Prakash A | 1998 | J Am Coll Cardiol1998，32，： | 1 |
| 8 | Role of foetomaternal haemorrhage in the causation of anaemia in the newborn infant显示文摘 | Saini RK Walia BN Prakash A | 1973 | Indian Pediatr1973，10，12： | 1 |
| 9 | Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA显示文摘 | Lowe B A Shiva Prakash N Way M | 2009 | Transgenic Research2009，18，6： | 1 |
| 10 | Du-loxetine for the treatment of major depressive disorder; acloser look at efficacy and safety data across the approveddose range 显示文摘 | Mallinckrodt CH Prakash A Andorn AC | 2006 | J Psychiatr Res2006，40，4： | 1 |
| 11 | Characterization of commercial Li-ion batteries using electrochemical-calorimetric measurements显示文摘 | HALLAJ S A PRAKASH J SELMAN J R | | 0，，： | 1 |
| 12 | Tuning the reactivity of energetic nanoparticles by creation of a core-shell nanostructure显示文摘 | Prakash A Mccormick A V Zachariah M R | | 0，，07： | 1 |
| 13 | New mineral occurrences and mineralization processes:Wuda coal-fire gas vents of Inner Mongolia显示文摘 | Stracher G B Prakash A Schroeder P | 2005 | American Mineralogist2005，90，1112： | 1 |
| 14 | Catalytic study of methanol-to-olefins conversion in four small-pore silicoalumino- phosphate molecular sieves : Influence of the structural type, nickel incorporation, nickel location, and nickel concentration 显示文摘 | Djieugoue Marie-Ange Prakash A M Kevan Larry | 2000 | Journal of Physical Chemistry B2000，104，27： | 1 |
| 15 | Effect of ingredients on rheological,nutritional and quality characteristics of high protein,high fibre and low carbohydrate bread显示文摘 | DHINDA F LAKSHMI A J PRAKASH J | 2012 | Food and Bioprocess Technology2012，5，8： | 1 |
| 16 | Numerical solution of temperature- dependent thermal conductivity problems using a meshless method 显示文摘 | Singh A Singh I V Prakash R | 2006 | Numerical Heat Transfer Part B: Fundamentals2006，50，2： | 1 |
| 17 | Dual site atrial pacing for the acute and chronic prevention of atrial fibrillation:A prospective study显示文摘 | Saksena S Hill M | 1995 | Am Coll Cardiol1995，25，： | 1 |
| 18 | Study and development of conducting polymer-based electrochromic display devices显示文摘 | Somani Prakash Mandale A B Radhakrishnan S | 2000 | Acta Materialia2000，48，11： | 1 |
| 19 | Prevention of recurrent atrial fibrillation with chronic dual-site right atrial pacing显示文摘 | Seksena S Prakash A Hill M | 1996 | J Am Coll Cardiol1996，28，： | 1 |
| 20 | Aromatic Nitration With Nitric Acid/Trifluoromethanesulfonic Anhydide显示文摘 | George A Olak V Prakash Reddy G K Surya Prakash | 1992 | Synthesis1992，11，： | 1 |
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