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| 1 | Leukemia:研究鉴定出与34种AML白血病亚群相关联的基因突变显示文摘针对成年急性髓细胞白血病(acute myeloid leukemia,AML)患者的一项新的大型研究将80种癌症相关基因突变与5种AML亚型相关联。这5种AML亚型可通过特异性的染色体异常加以确定。在未来,这些发现可能有助指导基因突变测试和治疗决策。 | A-K Eisfeld K Mrózek J Kohlschmidt D Nicolet S Orwick C J Walker K W Kroll J S Blachly A J Carroll J E Kolitz B L Powell E S Wang R M Stone A de la Chapelle J C Byrd C D Bloomfield | 2017 | 现代生物医学进展2017，17，15： | 7 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 4 | Simultaneous Measuremertl of PWB and Chip Package Warpage Using Automatielmage Segmentalion显示文摘 | Reinhard E Powell Ume I Charles | 2007 | 1EEE TRANSACTIONS ON COMPONENTS AND PACKAGING TECHNOLOGIES2007，60，3： | 1 |
| 5 | The influence of cytokinins on in vitro shoot development of 'Concord Grape'显示文摘 | Pool R M Powell L E | 1975 | J Amer Soc Hort Sci1975，100，2： | 1 |
| 6 | Gene length and codon us age bias in Drosophila raelanogaster, SaccharomS, ces cerevisiae and Escherichia coli显示文摘 | Moriyama E N Powell J R | 1998 | Nucleic Acids Res1998，26，13： | 1 |
| 7 | Coupled finite-element codes for armature design显示文摘 | NEWILL J F POWELL J D ZIELINSKI A E | 2003 | IEEE Transac- tions on Magneties2003，39，1： | 1 |
| 8 | Emergy evaluation of the performance and sustainability of three agricultural systems with different scales and management显示文摘 | Martin J F Diemont S A W Powell E Stanton M Levy-Tacher S | 2006 | Agriculture Ecosystems&Environment2006，115，14： | 1 |
| 9 | Physical Properties of Polymer/clay Nanocomposites显示文摘 | Clois E Powell Gary W Beall | 2006 | Current Opinion in Solid State and Materials Science2006，0，： | 1 |
| 10 | A Model of Fishing Vessel Accident Probability显示文摘 | Jin D Powell H.L.K Thunberg E Solow A.R Talley W.K | | 0，，04： | 1 |
| 11 | Effect of elevated CO2 on photosynthesis,respiration and growth of perennial ryegrass显示文摘 | RYLE G J A POWELL C E TEUSON V | | 0，，： | 1 |
| 12 | Revisiting Managerial Perspectives on Dividend Policy 显示文摘 | Baker H K Powell G E Veit E T | 2002 | Journal of Economics & Finance2002，26，3： | 1 |
| 13 | A double blind, randomized, controlled trial of glutamine supplementation in parenteral nutrition显示文摘 | Powell T J Jamieson C P Bettany G E | 1999 | Gut1999，45，1： | 1 |
| 14 | Naturally occurring dicistronic criket paralysis virus RNA is regulated by two internal ribosome entry sites 显示文摘 | Wilson J E Powell M J Hoover S E | 2000 | Mol Cell Biol2000，20，14： | 1 |
| 15 | Influence of LCF overloads on combined HCF/LCF crack growth 显示文摘 | Byrne J Hall R F Powell B E | 2003 | International Journal of Fatigue2003，25，911： | 1 |
| 16 | Modern tidal rhythmites deposited in a deep-water estuaty显示文摘 | Cowan E A Cai J Powell R D | 1998 | Geo-marine Letters1998，18，： | 1 |
| 17 | Some alkaloid relationships among seedings and mature plants of FCT 显示文摘 | Moore E L Gwynn G R Powell N T | 1963 | Tobacco Science1963，7，： | 1 |
| 18 | Take it to the limit: Innovative CVaR applications to extreme credit risk measurement 显示文摘 | ALLEN D E POWELL R J | 2016 | European Journal of Operational Research2016，249，2： | 1 |
| 19 | Adding more beds to the emergency department or reducing admitted patient boarding times:which has a more significant influence on emergency department congestion?显示文摘 | Rahul K Khare E S Powell G R | 2008 | Ann Emerg Med2008，23，7： | 1 |
| 20 | Hair keratin:composition,structure and biogenesis显示文摘 | POWELL B C ROGERS G E | 1986 | Biology of the integument1986，2，： | 1 |
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