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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 3 | ^(99m)Tc-AMD3100:A novel potential receptor-targeting radiopharmaceutical for tumor imaging显示文摘The chemokine CXCR4 receptor is over-expressed in a wide variety of tumors.In this study,AMD3100,which was a prototype non-peptide antagonist of CXCR4 receptor,was labeled with ^(99m)Tc.^(99m)Tc-AMD3100 was verified by thin layer radio chromatography(TLRC).The tumor-localizing properties of ^(99m)Tc-AMD3100 were evaluated and proved in mice bearing Hep-G2 tumor xenograft.^(99m)Tc-AMD3100 was a promising,novel receptor-specific radiopharmaceutical with potential application in the imaging of human tumors. | Jin Ming Zhang~(a,),Jia He Tian~a,Tian Ran Li~a, Hai Yu Guo~a,Li Shen~b a Department of Nuclear Medicine,The PLA General Hospital,Beijing,100853,China b Peking University Health Science Center,Beijing,100191,China | 2010 | Chinese Chemical Letters2010，21，4： | 3 |
| 4 | A 14-bit intrinsic accuracy Q2 random walk CMOS DAC显示文摘 | Geert A Van der Plas M Vandenbussche J | 1999 | IEEE J Sol Sta Circ1999，34，12： | 1 |
| 5 | Biomarkers as biological indicators of xeno- biotic exposure 显示文摘 | Gil F Pla A | 2001 | J Appl Toxicol2001，21，： | 1 |
| 6 | Phylogeography of the Atlantic bonito (Sarda sarda) in the northern Mediterranean:the combined effects of historical vicariance,population expansion,secondary invasion,and isolation by distance显示文摘 | Vinas J Bremer J A Pla C | 2004 | Molecular Phylogenetics and Evolution2004，33，： | 1 |
| 7 | Association of DHEA-S and estradiol serum levels to symptoms of aging men显示文摘 | Ponholzer A Plas E Schatzl G | 2002 | Aging Male2002，5，4： | 1 |
| 8 | Com- position and functional properties of enriched fiber prod- ucts obtained from pumpkin ( Cucurbita moschata Duch- esne ex Poiret) 显示文摘 | de Escalada Pla M F Ponce N M Stortz C A | 2007 | LWT-Food Science and Technolo- gy2007，40，7： | 1 |
| 9 | Clustering-based hyperspectral band selection using information measures 显示文摘 | Martinez-Uso A Pla F Sotoca J M | 2007 | IEEE Transactions on Geoseience and Remote Sensing2007，45，12： | 1 |
| 10 | Prevalence of malnutrition in a mid-long term stay unit显示文摘 | Argente Pla M Garc侏a Malpartida K Len De Zayas B | 2014 | NutrHosp2014，31，2： | 1 |
| 11 | Phytoremediation of hydrocarbons-polluted agricultural soils显示文摘 | ANDREOTTI G PLA TA N PORTA A | | Phytoremediation Wet1ands and Sediments 200l0，6，5： | 1 |
| 12 | Prognostic significance of karyotype at diagnosisin childhood acute lymphoblastic leukemia 显示文摘 | Plas VD Hahlen K Hagereijer A | 1992 | Leukemia1992，6，3： | 1 |
| 13 | Finite elements analysis and muhiobjective optimization: A way to reduce material and manufacturing cost 显示文摘 | Sttnchez-Caballero S Sell6s M A Pla R | 2012 | AIP Conference Proceedings2012，1431，1： | 1 |
| 14 | Multiple roles of protein kinase A in arachidonic acid mediated Ca2+ entry and tumor-derived human endothelial cell migration 显示文摘 | Fiorio Pla A Genova T Pupo E | 2010 | Molecular Cancer Research2010，8，11： | 1 |
| 15 | Preparative purification of soybean agglutinin by affinity chromatography and its immobilization for polysaccharide isolation显示文摘 | FRANCO FRAGUAS L PLA A FERREIRA F | 2003 | Journal of Chromatography2003，790，： | 1 |
| 16 | Sensitivity of osteocytes to biomechanical stress in vitro 显示文摘 | Klein-Nulend J Van Der Plas A Semeins C M | 1995 | FASEB J1995，9，： | 1 |
| 17 | Petrologic and stable isotope constraints on hydrothermal alteration and serpentinization of the EPR shallow mantle at Hess Deep (Site 895) 显示文摘 | FRUH-GREEN G L PLAS A LECUYER C | 1996 | Proceedings of Ocean Drilling Program: Scientific Results1996，147，： | 1 |
| 18 | Sen-sitivity of osteocytes to biomechanical stress in vitro 显示文摘 | Klein-Nulend J van der Plas A Semeins CM | 1995 | FASEB J1995，9，： | 1 |
| 19 | Canonical transient receptor potential 1 plays a role in basic fibroblast growth factor (bFGF)/FGF receptor-1-induced Ca^2+ entry and embryonic rat neural stem cell proliferation 显示文摘 | Fiorio Pla A Maric D Brazer SC | 2005 | J Neurosci2005，25，： | 1 |
| 20 | Natural and human-induced hypoxia and consequences for coastal areas: synthesis and future development显示文摘 | Zhang J. Gilbert D. Gooday A. J. Levin L. Naqvi S. W. A. Middelburg J. J. Scranton M. Ekau W. Pe?a A. Dewitte B. Oguz T. Monteiro P. M. S. Urban E. Rabalais N. N. Ittekkot V. Kemp W. M. Ulloa O. Elmgren R. Escobar-Briones E. Van der Plas A. K | 2010 | Biogeosciences2010，，68： | 1 |
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