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| 1 | Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease显示文摘AIM:To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease(IBD) .METHODS:Sixty-one adolescents(mean age 15 years,SD ± 4.13) were included in the study.Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions.The number of microbes belonging to the same group was calculated per weight of collected tissue.The mucus thickness in frozen samples was measured under a fluorescent microscope.RESULTS:The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases.Streptococcus spp.were predominant in the inflamed mucosa of Crohn's disease(CD) patients(80% of all bacteria) ,and Lactobacillus spp.were predominant in ulcerative colitis patients(90%) .The differences were statistically significant(P = 0.01-0.001) .Lower number of bifidobacteria was observed in the whole IBD group.A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and,to a lesser extent,of Streptococcus in biopsies from CD patients.The mucus layer in the inflamed sites was significantly thinner as compared to controls(P = 0.0033) and to non-inflamed areas in IBD patients(P = 0.031) .CONCLUSION:The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases,their numbers decreased in relation to higher clinical and endoscopic activity of the disease. | Krzysztof Fyderek Magdalena Strus Kinga Kowalska-Duplaga Tomasz Gosiewski Andrzej Wedrychowicz Urszula Jedynak-Wasowicz Malgorzata Sladek Stanislaw Pieczarkowski Pawel Adamski Piotr Kochan Piotr B Heczko | 2009 | World Journal of Gastroenterology2009，15，42： | 14 |
| 2 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 3 | Effect of accelerated ageing on the performance of high voltage carbon/carbon electrochemical capacitors in salt aqueous electrolyte显示文摘 | Paula Ratajczak Krzysztof Jurewicz Piotr Skowron Qamar Abbas Francois Béguin | 2014 | Electrochimica Acta2014，，： | 3 |
| 4 | Requirement for cyclin D3 in germinal center formation and function显示文摘第二等的淋巴的纸巾的幼芽的中心(GC ) 对装高亲密关系的体液的有免疫力的回答批评。当多样化他们的抗体基因时,在 GC 以内的 B 房间经历快速的同种细胞的扩大和选择。尽管 GC B 房间采用一个唯一的 proliferative 程序提供这些进程,这通常被相信,很少联系 GC 的房间周期怎么被安排被知道。D 类型 cyclins 组成使房间能对生理的变化作出回应的房间周期引擎的一个重要部件。房间类型 -- 并且 D 类型 cyclins 的发展阶段特定的角色被描述了,但是 cyclin D 要求没在 GC 反应期间被探讨。在这研究,我们报导 cyclin D3 为切换的增长和 Ig 班大部分是非必需的在 vitro 激活的 B 房间。相反,在 Ccnd3 的 GC 开发 ?/ ?老鼠显著地被损害,作为是 T 房间依赖者抗体反应。在 GC 以内,尽管切换, unswitched B 房间被 cyclin D3 inactivation 影响, IgM?水池更严重地被减少。有趣地尽管有 cyclin D2 表示的补偿增加, Ccnd3 的一个重要数字 ?/ ?GC B 房间处于静止 G0 状态积累。最后,尽管 cyclin D3 inactivation 没在 GC B 房间破坏 BCL6 表示,支持 BCL6 overexpression 的效果完全堵住了 GC,建议 cyclin D3 调整 GC 形成的 BCL6 下游地行动。这是 cyclin D3 玩的第一示范在 B 房间开发的 GC 阶段的一个重要、唯一的角色。 | Jonathan U Peled J Jessica Yu Jeganathan Venkatesh Enguang Bi B Belinda Ding Melissa Krupski-Downs Rita Shaknovich Piotr Sicinski Betty Diamond Matthew D Scharff B Hilda Ye | 2010 | Cell Research2010，20，6： | 3 |
| 5 | Carbonylation of aromatic dinitro compounds with carbon monoxide to respective dicarbamates in the presence of the PdCl2/Fe/I2/Py catalytic system 显示文摘 | Monika K Jadwiga S Piotr B | 2009 | Journal of Molecular Catalysis A: Chemical2009，303，12： | 1 |
| 6 | Moving least squares response surface approximation: Formulation and metal forming applications显示文摘 | PIOTR B HAKIM N ALAIN R | 2006 | Computers & Structures2006，83，1718： | 1 |
| 7 | Complexes of Schiff base of gossypol with 5-hydroxy-3-oxapentylamine and some monovalent cations studied by ESI-MS as well as PM5 semiempirical methods显示文摘 | Piotr P Grzegorz B Grzegorz S | 2003 | Journal of Molecular Structure2003，654，： | 1 |
| 8 | A thermodynamic analysis of refrig- erants: Performance limits of the vapor compression cycle 显示文摘 | Piotr A D Steven J B | 2014 | International Journal of Refrigeration2014，，38： | 1 |
| 9 | Decomposition of microcystin-LR by Fenton oxidation 显示文摘 | Piotr G Zbigniew L Tadeusz B | 2001 | Toxicon2001，39，： | 1 |
| 10 | Comparison of spectral irradiance standards used to calibrate shortwave radiometers and spectral radiometers 显示文摘 | PIOTR W K JOSEPH J M JERRY L B | 1999 | Applied Optics1999，38，12： | 1 |
| 11 | Moving least squares response surface approximation: Formulation and metal forming applicatons显示文摘 | PIOTR B HAKIM N ALAIN R | 2005 | Computers and Structures2005，83，： | 1 |
| 12 | Reversed-phase liquid chromatographic method for the determination of selected room- temperature ionic liquid cations 显示文摘 | Piotr S Anja M Peter B | 2003 | Journal of Chromatography A2003，993，： | 1 |
| 13 | Comparison of spectral irradiance standards used to calibrate shortwave radiometers and spectral radiometers 显示文摘 | PIOTR W K JOSEPH J M JERRY L B | 1999 | Applied Optics1999，38，12： | 1 |
| 14 | A three-dimensional finite element model of round window membrane vibration before and after stapedotomy surgery显示文摘 | Monika K Piotr M Pawe?B | 2013 | Biomech Model Mechanobiol2013，12，： | 1 |
| 15 | Treatment of graft-versus-host disease with naturally occurring T reg ulatory cells 显示文摘 | Piotr T Dukat-Mazurek A Maria B | 2013 | Bio Drugs2013，27，60： | 1 |
| 16 | Interleukin - 6 gene polymor- phism and faster progression to end-stage renal failure in chronic glomerulonephritis 显示文摘 | Monika B Lucyna J Piotr K | 2007 | Translat Res2007，15，2： | 1 |
| 17 | Organoposphonate utilization by the wild-type strain of Penicillium notatum 显示文摘 | Bozena B Piotr W Teresa K L | 1995 | Applied and Environmental Microbiology1995，61，8： | 1 |
| 18 | Territorial Keys for Bringing Closer the Territorial Agenda of the EU and Europe 2020显示文摘 | Jacek Zaucha Tomasz Komornicki Kai B?hme Dariusz ?wi?tek Piotr ?uber | 2014 | European Planning Studies2014，，2： | 1 |
| 19 | Phosphoric amides, ^15N NMR study of the P-N Bonding in acyclic and cyclic compounds显示文摘 | Agnes M M Tomasz A M Piotr B | 1997 | Magnetic Resonance In Chemistry1997，35，： | 1 |
| 20 | Association of the VEGF gene polymorphism with diabetic retinopathy in type 2 diabetes patients 显示文摘 | Monika B Piotr K Iwona B G | 2007 | Nephrol Dial Transplant2007，22，3： | 1 |
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