|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Disease Risks of Childhood Obesity in China显示文摘Objective To estimate the relative risks of dyslipidemia, hypertension, diabetes mellitus, and metabolic syndromes among overweight and obese Chinese children compared with their normal weight counterparts. Methods Overweight and obesity were defined by age- and sex-specific BMI classification reference for Chinese children and adolescents. Pediatric metabolic syndrome (MetS) and each risk factor for MetS were defined using the criteria for US adolescents. Definition of hyper-TC, LDL, and dyslipidemia for adults was applied as well. General linear model factor analysis and chi-square test were used to compare the difference in metabolic indicators among normal weight, overweight, and obese groups. Multiple logistic regression analysis was performed to estimate the odds ratio of metabolic abnormalities between obesity, overweight, and normal weight children, after adjustment for living area, family economic level, age, sex, and daily exercise time and TV watching time, as well as different dietary indices in the model. Results Significant increases in blood lipids, glucose, and blood pressure were found among overweight and obese children as compared with their counterparts with normal weight. By applying WGOC-recommended BMI classification, the risks for hypertriglyceridemia, low HDL and dyslipidemia among overweight children were 1.9, 1.4, and 1.5 times, and was 3.3, 1.5, and 1.8 times among obese groups compared to their counterparts with normal weight after adjustment for age, sex, region, socioeconomic status, physical activity, and dietary intakes. The overweight and obese children (15-17.9 years) had a high-risk of developing hypertension, which was 2.3 and 2.9times higher than their counterparts with normal weight. Above 90% obese adolescents had abdominal obesity, while less than1% normal weight ones had abdominal obesity. No obese adolescents were free from any risk factors for MetS, while 36.9% of normal weight adolescents were from the risk factors. 83.3% obese boys and all obese girls had metabolic syndrome, while only15.5% normal weight boys and 18.8% normal weight girls had metabolic syndrome. Four risk factors for metabolic syndrome were found in 8.3% obese boys while none in normal weight boys and girls. The prevalence of MetS among normal weight,overweight, and obesity groups was 1.5%, 18.3%, and 38.1% respectively. Conclusion The cardiovascular disease (CVD)risk factors are clustered in obese Chinese children. Our observations strongly suggest that efforts should be made to prevent the onset of overweight and its associated diseases during early childhood. | YAN-PING LI XIAO-GUANG YANG FENG-YING ZHAI JIAN-HUA PIAO WEN-HUA ZHAO JIAN ZHANG GUAN-SHENG MA | 2005 | Biomedical and Environmental Sciences2005，18，6： | 70 |
| 2 | Altered trends in carbon uptake in China’s terrestrial ecosystems under the enhanced summer monsoon and warming hiatus显示文摘The carbon budgets in terrestrial ecosystems in China are strongly coupled with climate changes.Over the past decade,China has experienced dramatic climate changes characterized by enhanced summer monsoon and decelerated warming.However,the changes in the trends of terrestrial net ecosystem production(NEP)in China under climate changes are not well documented.Here,we used three ecosystem models to simulate the spatiotemporal variations in China's NEP during 1982–2010 and quantify the contribution of the strengthened summer monsoon and warming hiatus to the NEP variations in four distinct climatic regions of the country.Our results revealed a decadal-scale shift in NEP from a downtrend of–5.95 Tg C/yr^2(reduced sink)during 1982–2000 to an uptrend of 14.22 Tg C/yr^2(enhanced sink)during 2000–10.This shift was essentially induced by the strengthened summer monsoon,which stimulated carbon uptake,and the warming hiatus,which lessened the decrease in the NEP trend.Compared to the contribution of 56.3%by the climate effect,atmospheric CO2 concentration and nitrogen deposition had relatively small contributions(8.6 and 11.3%,respectively)to the shift.In conclusion,within the context of the global-warming hiatus,the strengthening of the summer monsoon is a critical climate factor that enhances carbon uptake in China due to the asymmetric response of photosynthesis and respiration.Our study not only revealed the shift in ecosystem carbon sequestration in China in recent decades,but also provides some insight for understanding ecosystem carbon dynamics in other monsoonal areas. | Honglin He Shaoqiang Wang Li Zhang Junbang Wang Xiaoli Ren Lei Zhou Shilong Piao Hao Yan Weimin Ju Fengxue Gu Shiyong Yu Yuanhe Yang Miaomiao Wang Zhongen Niu Rcmg Ge Huimin Yan Mei Huang Guoyi Zhou Yongfei Bai Zongqiang Xie Zhiyao Tang Bingfang Wu Leiming Zhang Nianpeng He Qiufeng Wang Guirui Yu | 2019 | National Science Review2019，6，3： | 24 |
| 3 | Human adipose-derived mesenchymal stem cells: a better cell source for nervous system regeneration显示文摘 | Han Chao Zhang Liang Song Lin Liu Yang Zou Wei Piao Hua Liu Jing | 2014 | Chinese Medical Journal2014，，2： | 20 |
| 4 | The effects of simulated nitrogen deposition on extracellular enzyme activities of litter and soil among different-aged stands of larch显示文摘Aims Nitrogen(N)addition could affect the rate of forest litter and soil organic matter decomposition by regulating extracellular enzyme activity(EEa).The impact of N addition on EEa may differ across different age stands with different organic matter quality.We were interested in whether the impact of N addition on EEa in litter and mineral soil during the growing season was dependent on stand age of a larch plantation in North China.Methods We added three levels of N(0,20 and 50 kg N ha^(−1) year^(−1))in three age stands(11,20 and 45 years old)of Larix principis-rupprech-tii plantation in North China.We measured potential activities of β-1,4-glucosidase(b),cellobiohydrolase(Cb),β-1,4-N-acetyl-glucosaminidase(Nag)and phenol oxidase(Po)in litter(organic horizon)and mineral soil(0-10 cm)during the second growing sea-son after N amendment.We also measured C and N concentrations,microbial biomass C and N,and KCl-extractable ammonium and nitrate in both litter and mineral soil.Important Findings We observed unimodal patterns of EEa during the growing season in all three stands,consistent with the seasonal variations of soil temperature.stand age had a strong effect on EEa in both litter and mineral soil,and this effect differed between litter and mineral soil as well as between different enzymes.N addition did not significantly affect the activities of b or Cb but significantly suppressed the activity of Nag in litter.We also found stand age-specific responses of Po activity to N addition in both litter and mineral soil.N addition suppressed Po activity of the high C:N ratio litters in 20-and 45-year-old stands but had no significant effect on Po activity of the low C:N ratio litter in 11-year-old stand.moreover,N addition inhibited Po activity of the high C:N ratio soil in 20-year-old stand but had no significant impact on Po activity of the low C:N ratio soils in 11-and 45-year-old stands.overall,stand age had a greater effect on EEa in litter and mineral soil compared to 2 years of N addition.moreover,the effect of N addition on Po activity is stand age dependent,which may affect the long-term soil carbon storage in this forest. | Yuecun Ma Biao Zhu Zhenzhong Sun Chuang Zhao Yan Yang Shilong Piao | 2014 | Journal of Plant Ecology2014，7，3： | 14 |
| 5 | Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1显示文摘Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis. | Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai Han-Ming Shen Jigang Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 14 |
| 6 | Relationship between genetic polymorphism of cytochrome P450IIE1 and fatty liver显示文摘AIM: To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.METHODS: Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients without fatty liver and 20 normal controls. Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst I and Rsa I.RESULTS: The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6 %), obese fatty liver (38.5 %), or diabetic fatty liver (33.3 %) was significantly lower than that of the corresponding patients without fatty liver (100 %, 100 % and 80 % respectively), while the frequency of C2 genes, including C1/C2 and C2/C2, was significantly higher (71.4 %/0 %, 61.5 %/0 %, and 66.7 %/20 %) (P<0.01). The frequency distribution of the above genes of non-fatty liver patients (100 %/0, 100 %/0, and 80 %/20 %) was not significantly different from that of the normal controls (85 %/15 %) (P>0.05).CONCLUSION: The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver. Besides, C2 gene may play a key role in the pathogenesis of fatty liver. | Yun-Feng Piao Jing-Tao Li Yang Shi, Department of Gastroenterology, First Hospital, Jilin University, Changchun 130021, Jilin Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 12 |
| 7 | Inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell proliferation显示文摘AIM: To study the inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell line SMMC-7721and to explore the mechanism of its effect.METHODS: SMMC-7721 cells were divided into two groups, one treated with all-trans retinoic acid (ATRA) for 5 days and the other as a control group. Light microscope and electron microscope were used to observe the morphological changes. Telomerase activity was analyzed with silver-stained telomere repeated assay protocal (TRAP). Expression of Caspase-3 was demonstrated with western blot.RESULTS: ATRA-treated cells showed differentiation features including small and pyknotic nuclei, densely stained chromatin and fewer microvilli. Besides, ATRA could inhibit the activity of telomerase, promote the expression of Caspase-3 and its activation.CONCLUSION: Telomerase activity and Caspase-3expression are changed in human hepatocellular carcinoma cell line SMMC-7721 treated with all-trans retinioc acid.The inhibition of telomerase activity and the activation of Caspase-3 may be the key steps through which ATRA inhibits the proliferation of SMMC-7721 cell line. | Yun-Feng Piao Yang Shi Pu-Jun Gao Department of Gastroenterology,the First Hospital of Jilin University,Changchun,130021,Jilin Province,China | 2003 | World Journal of Gastroenterology2003，9，9： | 9 |
| 8 | Method of coupled mode for long-range bottom reverberation显示文摘The theory of coupled mode is used for modeling the long-range bottom reverberation in shallow water caused by bottom roughness. The distant bottom reverberation level and spatial coherence of impulsive source are both derived. The results agree with those from the classical reverberation model, and are compared with the experimental data. The influence of source bandwidth and the distance between sources and receivers on the intensity of bottom reverberation are particularly discussed. The method is shown to be available for both the monoand the bi-static cases. | GAO Bo , YANG ShiE, PIAO ShengChun & HUANG YiWang National Laboratory of Underwater Acoustic Technology,Harbin Engineering University, Harbin 150001, China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，12： | 9 |
| 9 | FKF1 F-box protein promotes flowering in part by negatively regulating DELLA protein stability under long-day photoperiod in Arabidopsis^FA显示文摘FLAVIN-BINDING KELCH REPEAT F-BOX 1(FKF1)encodes an F-box protein that regulates photoperiod flowering in Arabidopsis under long-day conditions(LDs).Gibberellin(GA)is also important for regulating flowering under LDs.However,how FKF1 and the GA pathway work in concert in regulating flowering is not fully understood.Here,we showed that the mutation of FKF1 could cause accumulation of DELLA proteins,which are crucial repressors in GA signaling pathway,thereby reducing plant sensitivity to GA in flowering.Both in vitro and in vivo biochemical analyses demonstrated that FKF1 directly interacted with DELLA proteins.Furthermore,we showed that FKF1 promoted ubiquitination and degradation of DELLA proteins.Analysis of genetic data revealed that FKF1 acted partially through DELLAs to regulate flowering under LDs.In addition,DELLAs exerted a negative feedback on FKF1 expression.Collectively,these findings demonstrate that FKF1 promotes flowering partially by negatively regulating DELLA protein stability under LDs,and suggesting a potential mechanism linking the FKF1 to the GA signaling DELLA proteins. | Jindong Yan Xinmei Li Bingjie Zeng Ming Zhong Jiaxin Yang Piao Yang Xin Li Chongsheng He Jianzhong Lin Xuanming Liu Xiaoying Zhao | 2020 | Journal of Integrative Plant Biology2020，62，11： | 8 |
| 10 | Bevacizumab plus capecitabine and cisplatin in Chinese patients with inoperable locally advanced or metastatic gastric or gastroesophageal junction cancer: randomized, double-blind, phase III study (AVATAR study)显示文摘 | Lin Shen Jin Li Jianming Xu Hongming Pan Guanghai Dai Shukui Qin Liwei Wang Jinwan Wang Zhenzhou Yang Yongqian Shu Ruihua Xu Lei Chen Yunpeng Liu Shiying Yu Lilian Bu Yongzhe Piao | 2015 | Gastric Cancer2015，，1： | 7 |
| 11 | Relatively High Bone Mineral Density in Chinese Adolescent Dancers Despite Lower Energy Intake and Menstrual Disorder显示文摘Objective The effect of dietary restriction, intense exercise and menstrual dysfunction on bone mineral density remains controversial. The aim of this study was to assess the skeletal health status and relationship between bone mineral density and nutrient intake, menstrual status, estrogen level and other factos in Chinese adolescent dancers. Methods Sixty dancers and 77 healthy controls underwent measurements of bone density, body composition, and estrogen level. Nutrient intake, menstrual status and physical activity were assessed with questionnaires. The correlation between these factors were analyzed. Results The dancers under study had a significantly lean body mass index (18.3±1.4 kg/m2 vs. 21.7±3.1 kg/m2), lower percentage of body fat (0.25±0.05 vs. 0.34±0.04) and later age at menarche (14.0±0.9 y vs. 13.0±1.3 y), and the estrogen level, daily calorie and fat intake in them were also lower than in the controls. All the dancers undertook intensive physical activity every day and up to 69% of them suffered from irregular menarche. Yet they had relatively high BMD and BMC of the total body and legs than the controls after adjusting for BMI and age. Site-specific BMD was positively correlated to BMI, body composition and training hours per week and negatively correlated to the age at menarche and menstrual frequency. Conclusions The relatively high BMD and BMC of the dancers at the total body and legs were probably caused by high levels of weight-bearing physical activity. To ameliorate disordered eating, especially low energy intake might be helpful to prevent the Triad and to improve the bone health in adolescent dancers. | LI-CHEN YANG YAN LAN JING HU YAN-HUA YANG QIAN ZHANG ZHEN-WU HUANG JIAN-HUA PIAO | 2010 | Biomedical and Environmental Sciences2010，23，2： | 7 |
| 12 | Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa显示文摘瞄准:调查极端结构的本地化在综合的伊朗的银币尿钠排泄的肽(ANP ) 在在老鼠胃粘膜的综合 ANP 房间和微容器之间的房间和关系。方法:嵌入免疫电子显微镜学技术的 Immunohistochemistry 技术和帖子被用来在胃粘膜关于综合 ANP 房间的表达式和综合 ANP 房间的极端结构的本地化验证调查结果。组织化学技术和鞣质的酸铁的氯化物方法(TA-Fe 染色方法) 被用来在胃的不同区域揭示微容器密度和综合 ANP 房间的分发。结果:表示 ANP 的房间是局部性的,综合 ANP 房间作为 enterochromaffin (EC ) 被识别在胃粘膜的房间。综合 ANP 房间在胃的不同区域存在。在粘膜的百分比综合 ANP 房间在身体(21.6%+/-3.6%) 在宫底(46.7%+/-5.3%) 最大、在窦(40.1%+/-4.5%) 中间、最少。在综合 ANP 房间的百分比和在窦粘膜,然而并非在宫底或身体粘膜的微容器的密度之间有一种积极关系。结论:ANP 被 EC 房间在老鼠胃粘膜综合,并且综合 ANP 房间在胃的宫底是很稠密的。ANP 可以在胃肠道地区性的 autocrine 或 paracrine 管理者充当不仅,而且作为内分泌的规章的肽。 | Chun-hui LI Zong-wei YANG Zheng-ri YIN Zheng JIN De-gang XING Lian-hua PIAO Yong-chul KIM Wen-xie XU | 2006 | Acta Pharmacologica Sinica2006，27，2： | 7 |
| 13 | L-Carnitine protects against tacrolimus-induced renal injury by attenuating programmed cell death via PI3K/AKT/PTEN signaling显示文摘Reducing immunosuppressant-related complications using conventional drugs is an efficient therapeutic strategy.L-carnitine(LC)has been shown to protect against various types of renal injury.In this study,we investigated the renoprotective effects of LC in a rat model of chronic tacrolimus(TAC)nephropathy.SD rats were injected with TAC(1.5 mg·kg^(−1)·d^(−1),sc)for 4 weeks.Renoprotective effects of LC were assessed in terms of renal function,histopathology,oxidative stress,expression of inflammatory and fibrotic cytokines,programmed cell death(pyroptosis,apoptosis,and autophagy),mitochondrial function,and PI3K/AKT/PTEN signaling.Chronic TAC nephropathy was characterized by severe renal dysfunction and typical histological features of chronic nephropathy.At a molecular level,TAC markedly increased the expression of inflammatory and fibrotic cytokines in the kidney,induced oxidative stress,and led to mitochondrial dysfunction and programmed cell death through activation of PI3K/AKT and inhibition of PTEN.Coadministration of LC(200 mg·kg^(−1)·d^(−1),ip)caused a prominent improvement in renal function and ameliorated histological changes of kidneys in TAC-treated rats.Furthermore,LC exerted anti-inflammatory and antioxidant effects,prevented mitochondrial dysfunction,and modulated the expression of a series of apoptosis-and autophagy-controlling genes to promote cell survival.Human kidney proximal tubular epithelial cells(HK-2 cells)were treated with TAC(50μg/mL)in vitro,which induced production of intracellular reactive oxygen species and expression of an array of genes controlling programmed cell death(pyroptosis,apoptosis,and autophagy)through interfering with PI3K/AKT/PTEN signaling.The harmful responses of HK-2 cells to TAC were significantly attenuated by cotreatment with LC and the PI3K inhibitor LY294002(25μM).In conclusion,LC treatment protects against chronic TAC nephropathy through interfering the PI3K/AKT/PTEN signaling. | Hai-lan Zheng Hai-yue Zhang Chun-lian Zhu Hui-ying Li Sheng Cui Jian Jin Shang-guo Piao Yu-ji Jiang Mei-ying Xuan Ji-zhe Jin Ying-shun Jin Jung-pyo Lee Byung-ha Chung Bum-soon Choi Chul-woo Yang Can Li | 2021 | Acta Pharmacologica Sinica2021，42，1： | 7 |
| 14 | EXPERIMENTS AND SIMULATIONS OF A NEW DEVELOPED FORMING TECHNOLOGY-SHEAR-EXTRUSION PROCESS OF COMPONENTS WITH BRANCHES显示文摘Shear-extrusion process and its forming parumeters are proposed, whilst its laborsav- ing characteristic is utilized to forge large-size shutoff valve body on middle-due press. This new process is intended for the manufacture of large-size forged tubular components with branches on middle-due press. Experiments are carried out and processing parameters are obtained regarding the shear-extrusion process of a large-size shutoff valve body. Daformation and metal flow in the shear-extrusion process are inves- tigated. In orber to verify the laborsaving characteristic of this new process some contrastive experiments of extrusion force are performed between shear-extrusion and upsetting-extrusion for forming tubular components with branches. Based on rigid plastic FEM a plane-stain model is established to analyze shear-extrusion process of tubular components with branches. The analysis results by 2-dimensions PEM are comparatively quite well consistent with those of experiments. Both simulated and experimental results show that this new forming process is feasible for forging large-size tubular components with branches on middle-due press. | H.L. Yang, D.C. Kang, Z.L. Zhang, X.H. Piao and Z.D. Shi (National Die and Mold CAD Engineering Research Center, Shanghai Jiaotong University, Shanghai 200030, China) (School of Materials Sci. & Eng., Harbin Institute of Technology, Harbin 150001, China) | 2001 | Acta Metallurgica Sinica(English Letters)2001，14，3： | 6 |
| 15 | Changes in Activities of Glutamine Synthetase during Grain Filling and Their Relation to Rice Quality显示文摘Four japonica rice varieties differed in cooking and eating qualities were used in a pot experiment to study the relationship between the activities of glutamine synthetase during grain filling and rice quality. The activities of glutamine synthetase gradually increased and then declined as a single peak curve in the course of grain filling. The 15th day after heading was a turning point, before which the enzymatic activities in the inferior rice varieties with high protein content were higher than those in the superior rice varietie with low protein content, and after which it was converse. The activity of glutamine synthetase in grain was correlated with the taste meter value, peak viscosity and breakdown negatively at the early stage of grain filling whereas positively at the middle and late stages. Moreover, it was correlated with the protein content of rice grain and setback positively at the early stage and negatively at the middle and late stages. The correlation degree varied with the course of grain filling. From 15 days to 20 days after heading was a critical stage, in which the direction of correlation between the activity of glutamine synthetase and taste meter value and RVA properties of rice changed. | JIN Zheng xun QIAN Chun rong YANG Jing LIU Hai ying PIAO Zhong ze | 2007 | Rice science2007，14，3： | 6 |
| 16 | Deciphering impacts of climate extremes on Tibetan grasslands in the last fifteen years显示文摘Climate extremes have emerged as a crucial driver of changes in terrestrial ecosystems. The Tibetan Plateau, facing a rapid climate change, tends to favor climate extremes. But we lack a clear understanding of the impacts of such extremes on alpine grasslands. Here we show that extreme events(drought,extreme wet, extreme cold and extreme hot) occurred at a frequency of 0.67–4 months decade^(-1) during2001–2015, with extreme precipitation predominantly occurring in June-to-August and extreme temperatures in May. Drought and extreme wet cause opposite and asymmetric effects on grassland growth,with drought-induced reductions greater than increases due to extreme wet. Grassland responses to extreme temperatures, which predominantly occur in May, show a dipole-like spatial pattern, with extreme hot(cold) events enhanced(reduced) growth in the eastern plateau but slightly reduced(enhanced) growth in the western plateau. These opposite responses to extreme temperatures over the eastern plateau are explained by the possibility that the occurrence of extreme cold slows the preseason temperature accumulation, delaying the triggering of spring phenology, while extreme hot hastens the accumulation. In the western plateau, in contrast, positive responses to extreme cold are induced by accompanying high precipitation. Furthermore, high extremeness of climate events generally led to a much lower extremeness in growth response, implying that the Tibetan grasslands have a relatively high resistance to climate extremes. The ecosystem models tested could not accurately simulate grassland responses to drought and extreme temperatures, and require re-parameterization before trust can be placed in their output for this region. | Dan Liu Tao Wang Tao Yang Zhengjie Yan Yongwen Liu Yutong Zhao Shilong Piao | 2019 | Science Bulletin2019，64，7： | 5 |
| 17 | Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China显示文摘Background:The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly,with confirmed cases distributed across 31 counties.However,the epidemiology of brucellosis transmission has not been fully elucidated.To characterize the infecting strains isolated from humans,multiple-locus variable-number tandem repeats analysis(MLVA)and whole-genome single-nucleotide polymorphism(SNP)-based approaches were employed.Methods:Strains were isolated from two males blood cultures that were confirmed Brucella m elitensis positive following biotyping and MLVA.Genomic DNA was extracted from these two strains,and whole-genome sequencing was performed.Next,SNP-based phylogenetic analysis was performed to compare the two strains to 94 B.melitensis strains(complete genome and draft genome)retrieved from online databases.Results:The two Brucella isolates were identified as B.melitensis biovar 3(QH2019001 and QH2019005)following conventional biotyping and were found to have differences in their variable number tandem repeats(VNTRs)using MLVA-16.Phylogenetic examination assigned the 96 strains to five genotype groups,with QH2019001 and QH2019005 assigned to the same group,but different subgroups.Moreover,the QH2019005 strain was assigned to a new subgenotype,llj,within genotype II.These findings were then combined to determine the geographic origin of the two Brucella strains.Conclusions:Utilizing a whole-genome SNP-based approach enabled differences between the two B.m elitensis strains to be more clearly resolved,and facilitated the elucidation of their different evolutionary histories.This approach also revealed that QH2019005 is a member of a new subgenotype(Hj)with an ancient origin in the eastern Mediterranean Sea. | Zhi-Jun Zhao Ji-Quan Li Li Ma Hong-Mei Xue Xu-Xin Yang Yuan-Bo Zhao Yu-Min Qin Xiao-Wen Yang Dong-Ri Piao Hong-Yan Zhao Guo-Zhong Tian Qiang LiJian-Ling Wang Guang Tian Hai Jiang Li-Qing Xu | 2021 | Infectious Diseases of Poverty2021，10，2： | 5 |
| 18 | Viscoelasticity of repaired sciatic nerve by poly(lactic-co-glycolic acid) tubes显示文摘Medical-grade synthetic poly(lactic-co-glycolic acid) polymer can be used as a biomaterial for nerve repair because of its good biocompatibility,biodegradability and adjustable degradation rate.The stress relaxation and creep properties of peripheral nerve can be greatly improved by repair with poly(lactic-co-glycolic acid) tubes.Ten sciatic nerve specimens were harvested from fresh corpses within 24 hours of death,and were prepared into sciatic nerve injury models by creating a 10 mm defect in each specimen.Defects were repaired by anastomosis with nerve autografts and poly(lactic-co-glycolic acid) tubes.Stress relaxation and creep testing showed that at 7 200 seconds,the sciatic nerve anastomosed by poly(lactic-co-glycolic acid) tubes exhibited a greater decrease in stress and increase in strain than those anastomosed by nerve autografts.These findings suggest that poly(lactic-co-glycolic acid) exhibits good viscoelasticity to meet the biomechanical requirements for a biomaterial used to repair sciatic nerve injury. | Chengdong Piao Peng Li Guangyao Liu Kun Yang | 2013 | Neural Regeneration Research2013，8，33： | 4 |
| 19 | Total Energy Expenditure of 16 Chinese Young Men Measured by the Doubly Labeled Water Method显示文摘Objective Doubly labeled water (DLW) method is the gold standard for measuring total energy expenditure (TEE). We used this method to measure TEE in Chinese young men. Methods Sixteen healthy young men age 23±1 years with body mass index 22.0±1.4 kg/m 2 were recruited. TEE was measured by the DLW method, and basal energy expenditure (BEE) was determined by indirect calorimetry. We also conducted 24-h activity, energy balance and factorial approach to estimate energy requirements of the subjects. Results TEE of subjects by DLW method was 9.45±0.57 MJ/day (2258±180 kcal/day). The 24-h activity was 10.80±0.33 MJ/day (2582±136 kcal/day). The energy requirement, derived from energy balance observations, was 9.93±1.32 MJ/day (2373±315 kcal/day). The BEE of 6.65±0.28 MJ/day (1589±67 kcal/day), calculated by the adjusted Schofield equation, was significantly higher (P<0.001) than that measured by indirect calorimetry, 5.99±0.66 MJ/day (1433±158 kcal/day). The TEE derived from the factorial approach was 10.31±0.43 MJ/day (2463±104 kcal/day). Conclusion The TEE of Chinese young men measured by the DLW method was about 10% lower than the current recommended nutrient intake (RNI), suggesting that the RNI for Chinese men maybe overestimated. Further studies are warranted to determine the value of the estimated energy requirement. | ZHUO Qin SUN Rui GOU Ling Yan PIAO Jian Hua LIU Jian Min TIAN Yuan ZHANG Yu Hui YANG Xiao Guang | 2013 | Biomedical and Environmental Sciences2013，26，6： | 4 |
| 20 | Association between Adiponectin and Metabolic Syndrome in Older Adults from Major Cities of China显示文摘Objective To investigate the association between adiponectin and metabolic syndrome (MetS) and related diseases in older adults from major cities of China.Methods A total of 2 049 adults at the age of 60-96 years from 18 major cities of China were enrolled in the study.Plasma adiponectin and insulin concentrations were measured.Insulin resistance was assessed by homeostasis model assessment of insulin resistance (HOMA-IR).The definitions proposed by International Diabetes Federation (IDF)and American Heart Association/National Heart,Lung,and Blood Institute (AHA/NLHBI)were used to identify MetS.Results The adiponectin concentration increased with the advance of age and was higher in women than in men.The sex specific adiponectin concentration was inversely correlated with body mass index(BMI),waist circumference,diastolic blood pressure,triglycerides,glucose and fasting blood insulin,and positively correlated with HDL-C(P<0.001).The adiponectin concentration decreased with increasing MetS components.Compared with the 4th sex-specific adiponectin quartile,the odds ratio(OR)for prevalent MetS-IDF and MetS-AHA/NLHBI in subjects of the 1st quartile group was 3.25(95% CI:2.24,4.71)and 3.21(95% CI:2.26,4.55),respectively.The association was independent of age,sex,life-style factors,medication,family history of chronic diseases,BMI,and HOMA-IR.The OR for MetS was much higher than those of MetS components and its related diseases.Conclusion Adiponectin is strongly associated with MetS independent of insulin resistance and obesity in older adults from major cities in China.The adiponectin concentration is a useful predictor for the risk of MetS. | QIN ZHUO ZHI-QING WANG PING FU JIAN-HUA PIAO YUAN TIAN JIE XU XIAO-GUANG YANG | 2010 | Biomedical and Environmental Sciences2010，23，1： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员