|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 用荧光原位杂交技术构建高分辨率的DNA物理图谱显示文摘用荧光原位杂交技术构建高分辨率的DNA物理图谱钟筱波PaulF.FranszJ.HansdeJongPimZabel(荷兰瓦赫宁根农业大学分子生物学系)HighResolutionPhysicalMappingbyFluorescenceinsitu... | 钟筱波 Fran.,PF | 1997 | 遗传1997，19，3： | 22 |
| 2 | 使用注射器的脂肪抽吸塑形术显示文摘从1985年起我开始用注射器代替机器进行脂肪抽吸术。用这种办法,10秒钟可以抽满60ml的注射器。故认为机器抽吸得快的说法是错误的。 | Four.,PF 赵敏 | 1993 | 中华整形烧伤外科杂志1993，9，2： | 13 |
| 3 | Human hepatic sinusoidal endothelial cells can be distinguished by expression of phenotypic markers related to their specialised functions in vivo显示文摘The hepatic sinusoids are lined by a unique population of hepatic sinusoidal endothelial cells (HSEC), which is one of the first hepatic cell populations to come into contact with blood components. However, HSEC are not simply barrier cells that restrict the access of blood- borne compounds to the parenchyma. They are func- tionally specialised endothelial cells that have complex roles, including not only receptor-mediated clearance of endotoxin, bacteria and other compounds, but also the regulation of inflammation, leukocyte recruitment and host immune responses to pathogens. Thus understand- ing the differentiation and function of HSEC is critical for the elucidation of liver biology and pathophysiology. This article reviews methods for isolating and studying human hepatic endothelial cell populations using in vitro models. We also discuss the expression and functions of phe- notypic markers, such as the presence of fenestrations and expression of VAP-1, Stabilin-1, L-SIGN, which can be used to identify sinusoidal endothelium and to permit discrimination from vascular and lymphatic endothelial cells. | PF Lalor SM Curbishley S Shetty DH Adams | 2006 | World Journal of Gastroenterology2006，12，34： | 11 |
| 4 | Journal of Hepatology|肝细胞癌中的抗程序性死亡受体1耐药性的潜在分子机理显示文摘目前,迫切需要探索免疫检查点阻断治疗的耐受机制,并确定一种联合治疗策略以提高其在HCC治疗中的有效性。作为免疫豁免器官,肝脏含有大量巨噬细胞,包括常驻细胞(例如Kupffer细胞)和募集的巨噬细胞。浸润肿瘤组织的巨噬细胞也称为肿瘤相关巨噬细胞(TAM),在功能和表型上与交替激活的(M2样)巨噬细胞相似,在肿瘤进展、免疫逃逸和ICB治疗抵抗中起重要作用。 | 樊嘉 WEI CY ZHU MX ZHANG PF | 2022 | 临床肝胆病杂志2022，38，3： | 9 |
| 5 | 鲨鱼软骨粉对小鼠移植性肿瘤的抑制作用与微血管的关系显示文摘的观察鲨鱼软骨粉对小鼠移植性SRS(淋巴瘤)生长的影响与微血管密度的关系。方法肿瘤称重 ,切片用vWF免疫组化染色及微血管计算机扫描方法半定量。结果在已建立的小鼠SRS实体瘤模型上应用口服鲨鱼软骨粉治疗 ,发现54毫克/d治疗15d后肿瘤重量及微血管密度较对照组明显减少(P<0.05)。治疗组血管内皮生长因子(VEGF)与VEGF受体FIK -1表达明显减少(P<0.05)。结论上述剂量的鲨鱼软骨粉可抑制小鼠移植性SRS的生长。肿瘤微血管形成的抑制可能是鲨鱼软骨粉抗肿瘤机制之一 ,后者又与VEGF和FIK -1表达的抑制有关。 | 蔡峥嵘 钱睿哲 金惠铭 张国平 PF Davis Yi He | 2000 | 中国微循环2000，4，1： | 6 |
| 6 | 氯胺酮应用于临床麻醉新进展显示文摘氯胺酮是一种安全、速效的麻醉镇痛剂。注氯胺酮后血压上升,加快心率,引起流涎,流泪,出汗,骨骼肌紧张,无目的不随意运动,兴奋甚至谵妄。苯二氮(艹卓)类可预防氯胺酮引起的心血管反应、谵妄和苏醒期反应。应用静脉点滴法,麻醉者可更精确地调节给药速度,减少用药量。利用短效、低毒、蓄积少的静脉麻醉药(如阿芬太尼、咪唑二氮(艹卓)、异丙酚)和出于减少手术室麻醉气体污染,所以对全凭静脉麻醉技术越来越感兴趣。显然,在众多的静脉麻醉药中还没有一种完全理想的药物。但氯胺酮是唯一的、可单独应用的麻醉药。这是由于它有镇静、遗忘、镇痛和麻醉的特性。氯胺酮为水溶性,在溶液中稳定。 | White PF 程桥 | 1990 | 国外医学(麻醉学与复苏分册)1990，11，3： | 5 |
| 7 | The role of total mesorectal excision in the management of rectal cancer显示文摘 | Ridgway PF Darzi AW | | 0，，： | 3 |
| 8 | 氧化锆陶瓷的临床应用显示文摘 | Manicone PF Rossi Iommetti P Raffaelli L 刘小舟 吕培军 | 2008 | 中国实用口腔科杂志2008，1，1： | 3 |
| 9 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 10 | Adrenal masses:characterization with delayed contrast-enhanced CT 显示文摘 | Boland GWL Hahn PF Pena C | 1997 | Radiology1997，202，： | 2 |
| 11 | 发酵经济学显示文摘 | 许喜林 Stan.,PF | 1989 | 天津微生物1989，，1： | 2 |
| 12 | Angiopoietin-2,a natural antagonist for Tie2 that disrupts in vivo angiogenesis显示文摘 | Maisonpierre PC Suri C Jones PF | | 0，，： | 2 |
| 13 | Nonalcoholic steatohepatitis显示文摘 | Kumar KS Malet PF | | 0，，： | 2 |
| 14 | Angiopoietin-2,a natural antagonistor for Tie2 that distrupts in vivo angiogenesis显示文摘 | Maisonpierre PC Suri C Jones PF | 1997 | Science1997，277，4： | 2 |
| 15 | Oddi括约肌的动力和疾病显示文摘Oddi 括约肌(SO)为在十二指肠内环绕胆总管和胰管末端的括约肌Boyden 更系统地将它分为:1.胆总管括约肌;2.胰管括约肌;3.壶腹括约肌;4.中间纤维。胆总管括约肌和中间纤维可见于所有人,而仅1/3和1/6的人有胰管和壶腹括约肌,但这与动态研究结果不一致,胰管段括约肌即使经内镜作胆总管括约肌全切开术后仍存在。SO | Jensen PF 丁民谋 | 1992 | 国外医学(消化系疾病分册)1992，12，2： | 2 |
| 16 | 静脉滴注麻醉药和镇痛药的临床应用显示文摘吸入麻醉具有可控性强,能迅速改变麻醉深度,有较完善的给药装置,使用方便等优点。但因其传统药物的毒性,新药价格昂贵以及对空气的污染,随着有效静脉麻醉药的应用,静脉麻醉逐渐受到关注。传统的间断静注法使血药浓度迅速波动,造成麻醉或镇痛过深或过浅。连续静滴法给药准确,降低血药浓度的剧烈波动,减少用药量,改善麻醉状态,缩短恢复时间,缩小血-脑麻药浓度差,血流动力学稳定,副作用减少,可控性增强。一。 | White PF 吴云松 | 1990 | 国外医学(麻醉学与复苏分册)1990，11，3： | 2 |
| 17 | Use of alfentanil with propofol for nasotracheal intubation without neuromuscular block 显示文摘 | Coghlan SFE McDonald PF Csepregi G | 1993 | Br J Anaesth1993，70，1： | 1 |
| 18 | Middle cerebral artery thrombosis following blunt head trauma 显示文摘 | De Caro R Munari PF Parenti A | 1998 | Clin Neuropathol1998，17，： | 1 |
| 19 | Leukemia inhibilory factor rescues motoneurones fxom axotomy-induced cell death显示文摘 | Cheema SS RichardsLJ MurphyM Bartlett PF | 1994 | Neurore-port1994，5，： | 1 |
| 20 | Reproducibility of human kidney perfusion and volume determination with electron beam computed tomography 显示文摘 | Lerman LO Flickinger AL Sheedy PF | 1996 | Invest Radiol1996，31，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员