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| 1 | 瞬时受体电位M8离子通道对冷刺激诱导气道上皮细胞炎症反应的影响显示文摘目的探讨瞬时受体电位M8离子通道(TRPM8)在冷刺激诱导气道上皮细胞产生炎性因子过程中发挥的作用及相关信号转导机制。方法用冷空气(18℃)刺激人气道上皮16HBE细胞,以TRPM8通道特异性拮抗剂BCTC、TRPM8shRNA及蛋白激酶C(PKC)特异性抑制剂钙磷酸蛋白C为干预手段,将细胞分为对照组(37℃培养)、冷刺激组、冷刺激+BCTC组、冷刺激+转染TRPM8 shRNA组、冷刺激+转染对照shRNA组、冷刺激+钙磷酸蛋白C组。Western blot法检测TRPM8 shRNA转染对16HBE细胞合成TRPM8蛋白的干扰效率;钙离子成像技术测量前5组细胞内每10s间隔的相对Ca^2+浓度动态变化;ELISA法检测各组细胞分泌的白细胞介素(IL)-6、IL-8、肿瘤坏死因子(TNF)-α蛋白含量;实时荧光定量PCR检测各组细胞中IL-6、IL.8、TNF-α mRNA表达水平。结果冷刺激组细胞内相对Ca^2+浓度最高值为2.36±0.24,显著高于对照组的1.01±0.02(t=12.52,P〈0.01),冷刺激+BCTC组、冷刺激+转染TRPM8 shRNA组细胞内相对Ca^2+浓度降为1.47±0.17和1.26±0.12,显著低于冷刺激组(t值分别为6.69、9.12,均P〈0.01);冷刺激组的IL-6、IL-8、TNF-α的mRNA和蛋白含量分别为0.66±0.16、0.77±0.15、0.73±0.09、(92±13)ng/L、(125±22)ng/L、(88±12)ng/L,较对照组[0.37±0.08、0.32±0.07、0.48±0.10、(52±8)ng/L、(50±9)ng/L、(61±8)ng/L]显著升高(t值分别为3.20、5.36、3.36、5.24、6.26、3.74,均P〈0.05),冷刺激+BCTC组[分别为0.42±0.09、0.52±0.13、0.52±0.12、(72±8)ng/L、(92±14)ng/L、(68±11)ng/L]、冷刺激+转染TRPM8 shRNA组[分别为0.41±0.10、0.49±0.08、0.50±0.08、(60±12)ng/L、(89±14)ng/L、(68±11)ng/L]、冷刺激+钙磷酸蛋白C组[分别为0.40±0.07、0.44±0.09、0.47±0.08、(69±9)ng/L、(86±15)ng/L、(61±10)ng/L]较冷刺激组显著降低(均P〈0.05);冷刺激+转染对照shRNA组[分别为0.61±0.10、0.69±0.11、0.64±0.13、(89±13)ng/L、(118±20)ng/L、(79±13)ng/L]与冷刺激组比较差异无统计学意义(t值分别为0.48、0.79、1.12、0.35、0.43、1.00,均P〉0.05)。结论冷空气可通过激活气道上皮细胞上的TRPM8离子通道而诱导Ca^2+内流进而激活下游PKC信号通路,进而导致代表性炎性因子的表达及生成增多。 | 李敏超 Juliy M. Perelman Victor P. Kolosov 周向东 | 2011 | 中华结核和呼吸杂志2011，34，10： | 6 |
| 2 | 论强劲知识产权的软肋显示文摘在这篇引人注目的具有历史意义的研究中,作者阐释了对专利权和版权的争斗将如何破坏人类进步。在近几十年中,知识产权得到进一步的加强。作者承认,他并没有成型的解决方案,但是他认为追求如此强大的产权将产生悲剧性的后果。 | Micheal Perelman 孙宁 | 2005 | 经济资料译丛2005，，2： | 5 |
| 3 | 白细胞介素-13刺激大鼠气道黏液高分泌的分子机制显示文摘目的研究白细胞介素-13(IL-13)在体内对支气管/肺黏液分泌的作用并探讨其作用机制。方法所有SD大鼠被随机分为IL-13组、对照组和IL-13 plus SP600125组。Western检测大鼠支气管/肺组织黏蛋白(MUC)5AC,磷酸化细胞外信号调节激酶1/2(p-ERK1/2)和磷酸化c-Jun氨基端激酶1/2(p-JNK1/2)的表达变化,RT-PCR检测大鼠支气管/肺组织STAT4和STAT6 mRNA表达水平。EMSA检测通路下游作用因子FOXA2和激活蛋白1的表达。结果同对照组相比,IL-13刺激10 h后大鼠支气管/肺组织MUC5AC和p-JNK1/2表达升高,而p-ERK1/2表达无显著变化;使用SP600125阻断JNK通路表达后,MUC5AC表达减弱;IL-13刺激10 h后大鼠支气管/肺组织STAT4 mRNA表达无显著变化,STAT6 mRNA表达显著升高,EMSA提示IL-13刺激后大鼠支气管/肺组织FOXA2结合活性显著降低,而激活蛋白1结合活性没有明显变化。结论 IL-13能够刺激大鼠支气管/肺组织黏液分泌,其作用机制可能是通过调控STAT6-FOXA2信号通路。 | 江德鹏 Victor P Kolosov Juliy M Perelman 周向东 | 2011 | 南方医科大学学报2011，31，1： | 2 |
| 4 | The Poetry Hoax显示文摘This article is concerned with didactic hoaxes that are con structed to discredit their targets; the underlying question is whether a poetry hoax can do this. I compare the Sokal hoax with the Ern Malley hoax: in the first case (USA, 1996), an NYU physicist, Alan Sokal, wrote a bogus article claiming that the laws of physics should be seen as social constru cts; in the second case (Australia, 1943) two poets, James McAuley and Harold Stewart, concocted th e poems of an imaginary modernist, Ern Malley. In both cases the hoaxers realized their aim: Social Text, the magazine that published Sokal’s article, and Angry Penquins, the magazine th at celebrated the poems of Ern Malley, looked foolish. However, while the physics that Sokal used was bogus, what McAuley and Stewart had intentionally tried to write as bad poetry now reads as lively, comic and inventive. The poetry of ″Ern Malley″ now generates more interest than th e genuine poetry of McAuley and Stewart. Thus, while Malley is a hoax-poet, it doesn’t seem that th e hoaxers succeeded in writing hoax-poetry. | Bob Perelman | 2005 | 外国文学研究2005，27，2： | 2 |
| 5 | MiR-146a modulates neutrophil elastase-induced MUC5AC secretion from 16HBE human bronchial epithelial cells显示文摘 | Zhong T Perelman JM Kolosov VP | | 0，，02： | 1 |
| 6 | The dynamics of wave packets of highly-excited atoms and molecules显示文摘 | AVERBUKH I Sh PERELMAN N F | 1991 | Soviet Physics Uspekhi1991，，57： | 1 |
| 7 | Alternation of collagen compoition and cross- linking in keloid tissues显示文摘 | Di Cesare PE Cheung DT Perelman N | 1990 | Matrix1990，10，3： | 1 |
| 8 | The expression of three genes in primary non-small celt lung cancer is associated with metastatic spread to the brain显示文摘 | Grinberg-Rashi H Ofek E Perelman M | 2009 | Clin Cancer Res2009，15，5： | 1 |
| 9 | Influence of a- cute normovolaemie haemodilution on the dose-response rela- tionship,time-course of action and pharmacokineties of rocu- ronium bromide显示文摘 | Dahaba A Perelman S Moskowitz DM | 2006 | Br J Anaesth2006，97，4： | 1 |
| 10 | Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo显示文摘 | Zonios G Perelman L T Backman V | 1999 | Applied Optics1999，38，： | 1 |
| 11 | Detection of preinvasive cancer cells 显示文摘 | Backman V Wallace M B Perelman L T | 2000 | Nature2000，406，： | 1 |
| 12 | A Comparison of Parametric and Nonparametric Distance Functions: With Application to European Railways 显示文摘 | Coelli T J S Perelman | 1999 | European Journal of Operational Research1999，117，2： | 1 |
| 13 | Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii显示文摘 | Burg JL Perelman D Kasper LH | 1988 | J Immunol1988，141，： | 1 |
| 14 | Platelet-activating fac- tor, histamine, and tryptase levels in human anaphy- laxis显示文摘 | Vadas P Perelman B Liss G | 2013 | J Allergy Clin Immunol2013，131，1： | 1 |
| 15 | Aspergillosis in ostriches显示文摘 | Perelman B Kuttin E S | 1992 | Avian Pathol1992，21，： | 1 |
| 16 | Pediatric tumor cells express erythropoietin and a functional erythropoietin receptor that promotes angiogenesis and tumor cell survival 显示文摘 | Batra S Perelman N Luck RL | 2003 | Lab Invest2003，83，10： | 1 |
| 17 | Occupational Activities and Cognitive Reserve: A Frontier Approach Applied to the Survey of Health, Ageing and Retirement in Europe (SHARE)显示文摘 | Adam S Bay C Bonsang E Germain S Perelman S | 2006 | CREPP WP2006，，： | 1 |
| 18 | Disorders of orgasm and ejaculation in men显示文摘 | McMahon CG Abdo C Incrocci L Perelman M Rowland D Waldinger M Xin ZC | 2004 | Sex Med2004，1，1： | 1 |
| 19 | Aspergillosis in ostriches显示文摘 | B. Perelman E. S. Kuttin | 1992 | Avian Pathology1992，，1： | 1 |
| 20 | Pediatric tumor ceils express erythropoietin and a functional erythropoietin receptor that pro- motes angiogenesis and tumor cell survival 显示文摘 | Batra S Perelman N Luck LR | 2003 | Lab Invest2003，83，10： | 1 |
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