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| 1 | Relationship between Cognition Function and Hippocampus Structure after Long-term Microwave Exposure显示文摘Objective To analyze the effects of long-term microwave exposure on hippocampal structure and function in the rat.Methods Experiments were performed on 184 male Wistar rats(three exposure groups and a sham group).Microwaves were applied daily for 6 min over 1 month at average power densities of 2.5,5,and 10 mW/cm2.Learning and memory abilities were assessed by Morris water maze.High performance liquid chromatography was used to detect neurotransmitter concentrations in the hippocampus.Hippocampal structures were observed by histopathological analysis.Results Following long-term microwave exposure there was a significant decrease in learning and memory activity in the 7 d,14 d,and 1 m in all three microwave exposure groups.Neurotransmitter concentrations of four amino acids(glutamate,aspartic acid,glycine,and gamma-aminobutyric acid) in hippocampus were increased in the 2.5 and 5 mW/cm2 groups and decreased in the 10 mW/cm2 group.There was evidence of neuronal degeneration and enlarged perivascular spaces in the hippocampus in the microwave exposure groups.Further,mitochondria became swollen and cristae were disordered.The rough endoplasmic reticulum exhibited sacculated distension and there was a decrease in the quantity of synaptic vesicles.Conclusion These data suggest that the hippocampus can be injured by long-term microwave exposure,which might result in impairment of cognitive function due to neurotransmitter disruption. | ZHAO Li PENG Rui Yun WANG Shui Ming WANG Li Feng GAO Ya Bing DONG Ji LI Xiang SU Zhen Tao | 2012 | Biomedical and Environmental Sciences2012，25，2： | 27 |
| 2 | Effect of HCV NS_3 protein on P53 protein expression in hepatocarcinogenesis显示文摘NTRODUCTIONHepatocelularcarcinoma(HCC)isoneofthemostcommonhumancancersintheworld.Recently,theHCVinfectionwasfoundtobeanetiolo... | FENG De Yun, CHEN Rui Xue, PENG Yong, ZHENG Hui and YAN Ya Hui | 1999 | World Journal of Gastroenterology1999，5，1： | 25 |
| 3 | A hybrid dynamic programming-rule based algorithm for real-time energy optimization of plug-in hybrid electric bus显示文摘The optimization of the control strategy of a plug-in hybrid electric bus(PHEB) for the repeatedly driven bus route is a key technique to improve the fuel economy. The widely used rule-based(RB) control strategy is lacking in the global optimization property, while the global optimization algorithms have an unacceptable computation complexity for real-time application. Therefore, a novel hybrid dynamic programming-rule based(DPRB) algorithm is brought forward to solve the global energy optimization problem in a real-time controller of PHEB. Firstly, a control grid is built up for a given typical city bus route, according to the station locations and discrete levels of battery state of charge(SOC). Moreover, the decision variables for the energy optimization at each point of the control grid might be deduced from an off-line dynamic programming(DP) with the historical running information of the driving cycle. Meanwhile, the genetic algorithm(GA) is adopted to replace the quantization process of DP permissible control set to reduce the computation burden. Secondly, with the optimized decision variables as control parameters according to the position and battery SOC of a PHEB, a RB control is used as an implementable controller for the energy management. Simulation results demonstrate that the proposed DPRB might distribute electric energy more reasonably throughout the bus route, compared with the optimized RB. The proposed hybrid algorithm might give a practicable solution, which is a tradeoff between the applicability of RB and the global optimization property of DP. | ZHANG Ya Hui JIAO Xiao Hong LI Liang YANG Chao ZHANG Li Peng SONG Jian | 2014 | Science China(Technological Sciences)2014，57，12： | 20 |
| 4 | Detection of hepatitis C virus NS5 protein and genome in Chinese carcinoma of the extrahepatic bile duct and its significance显示文摘AIM To investigate the hepatitis C virus(HCV)infection in the tissues of carcinoma ofextrahepatic bile duct and study theircorrelation.METHODS HCV NS5 protein and HCV RNA weredetected by labeled streptavidin biotin(LSAB)method and in situ reverse transcriptionpolymerase chain reaction(IS-RT-PCR)insections of 51 cases of carcinoma ofextrahepatic bile duct and 34 cases of controlgroup(without malignant biliary disease).RESULTS In 51 cases of carcinoma ofextrahepatic bile duct,HCV NS5 protein wasdetected in 14(27.5%),which was clearlystained in the cytoplasm of cancer cell but not inthe nucleus or cell membrane.HCV RNA wasdetected in 18(35.4%),which was located inthe nucleus of cancer cell in 12 cases and in thecytoplasm in 6 cases.HCV NS5 protein and RNAcoexistence was found in 2 cases.In 34 cases ofcontrol group,HCV RNA was detected in 2(5.9%).HCV NS5 protein and RNA positive cellswere found either scattered or in clusters.CONCLUSION The prevalence of hepatitis C viral infection in the tissues of carcinoma ofextrahepatic bile duct was significantly higherthan in control group(X^2=9.808,P=0.002).The findings suggest a correlation between HCVinfection and carcinoma of extrahepatic bileduct,which is different from the traditionalviewpoint.HCV infection might be involved inthe development of carcinoma of extrahepaticbile duct. | Ming Yi Chen Zhi Qiang Huang Le Zhen Chen Ya Bing Gao Rui Yun Peng De Wen Wang | 2000 | World Journal of Gastroenterology2000，6，6： | 19 |
| 5 | Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer显示文摘AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (T3N1M0) colon cancer patients who underwent radical resection between January 1999 and May 2002 at the Cancer Center of Sun Yat-Sen University, Guangzhou, China. Immuno-histochemical analysis was performed to show infiltration of lymphocytes and expression of calreticulin in colon cancer. Association between calreticulin expression, infiltration of lymphocytes, and 5-year survival rate of patients was assessed. RESULTS: The expression level of calreticulin was lower in cancer nest than in its adjacent normal epithelium since 61.8% (42/68) of the samples were stained with calreticulin in colon cancer. The expression of calreticulin in colon cancer was associated with the infi ltration of CD45RO+ cells rather than with that of CD3+ cells. In addition, the stronger expression of calreticulin and the higher infiltration of CD3+ and CD45RO+ cells in colon cancer were associated with the higher 5-year survival rate of patients. CONCLUSION: Expression of calreticulin is associated with infiltration of T-cells, which implies that a low expression level of molecular marker may represent a new mechanism underlying immune escape in colon cancer. | Rui-Qing Peng, Ya Ding, Xing Zhang, Yi-Xin Zeng, Xiao- Shi Zhang, State Key Laboratory of Oncology in South China, Biotherapy Center, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Ying-Bo Chen, Zhi-Wei Zhou, State Key Laboratory of Oncology in South China, Department of Gastric and Pancreatic Oncology, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Rong Zhang, State Key Laboratory of Oncology in South China, Department of Endoscopy, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Xing-Juan Yu, Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China | 2010 | World Journal of Gastroenterology2010，16，19： | 16 |
| 6 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 7 | Electroacupuncture regulates the stress-injury-repair chain of events after cerebral ischemia/reperfusion injury显示文摘Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are released by damaged cells, which can lead to systemic stress reactions. Protecting the brain through repair begins with the stress-injury-repair signaling chain. This study aimed to verify whether acupuncture acts through this chain to facilitate effective treatment of ischemic stroke. Rat models of cerebral ischemia/reperfusion injury were established by Zea Longa's method, and injury sites were identified by assessing neurological function, 2,3,5-triphenyltetrazolium chloride staining, and hematoxylin-eosin staining. Electroacupuncture at acupoints Baihui(DU20) and Zusanli(ST36) was performed in the model rats with dilatational waves, delivered for 20 minutes a day at 2–100 Hz and an amplitude of 2 m A. We analyzed the blood serum from the rats and found that inflammatory cytokines affected the levels of adrenotrophin and heat shock protein 70, each of which followed a similar bimodal curve. Specifically, electroacupuncture lowered the peak levels of adrenocorticotrophic hormone and heat shock protein 70. Thus, electroacupuncture was able to inhibit excessive stress, reduce inflammation, and promote the repair of neurons, which facilitated healing of ischemic stroke. | Peng Shi Lin-lin Sun Yi-shuo Lee Ya Tu | 2017 | Neural Regeneration Research2017，12，6： | 14 |
| 8 | Serum levels of mi RNA in patients with hepatitis B virus-associated acute-on-chronic liver failure显示文摘Background: Hepatitis B virus(HBV)-associated acute-on-chronic liver failure(HBV-ACLF) is a lifethreatening condition and its exact pathophysiology and progression remain unclear. The present study aimed to assess the role of serum mi RNAs in the evaluation of HBV-ACLF and to develop a model to predict the outcomes for ACLF.Methods: Serum was collected from 41 chronic hepatitis B and 55 HBV-ACLF patients in addition to30 chronic asymptomatic HBV carriers as controls. The mi RNAs expressions were measured by real-time quantitative PCR(q-PCR). Statistical analyses were conducted to assess the ability of differentially expressed mi RNAs and other prognostic factors in identifying ACLF prognosis and to develop a new predictive model.Results: Real-time q-PCR indicated that serum miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p levels were significantly upregulated in ACLF patients compared to chronic hepatitis B and chronic asymptomatic HBV carriers patients. In addition, multivariate regression analyses indicated that Na+, INR, gastrointestinal bleeding and mi R-122-3 p are all independent factors that are reliable and sensitive to the prognosis of HBV-ACLF. Therefore, we developed a new model for the prediction of HBV-ACLF disease state: Y = 0.402 × Na+-1.72 × INR-4.963 × gastrointestinal bleeding(Yes = 0; No = 1)-0.278 ×(mi R-122-3 p) + 50.449. The predictive accuracy of the model was 95.3% and the area under the receiver operating characteristic curve(AUROC) was 0.847.Conclusions: Expression levels of these mi RNAs(miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p) positively correlate with the severity of liver inflammation in patients with ACLF and may be useful to predict HBV-ACLF severity. | Ya Wen Shi-Fang Peng Lei Fu Xiao-Yu Fu Da-Xian Wu Bing-Jie Liu De-Ming Tan Yi Ouyang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，2： | 13 |
| 9 | Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment显示文摘A couple with a proband child of GJB2(encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis(PGD), noninvasive prenatal testing(NIPT), and noninvasive prenatal diagnosis(NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2 c.235del C heterozygous embryo resulted in a singleton pregnancy. At the 13 th week of gestation, genomic DNA(g DNA) from the trio family and cell-free DNA(cf DNA) from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by invasive procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing impairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders. | XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu | 2015 | Science China(Life Sciences)2015，58，9： | 12 |
| 10 | Chloroquine enhances the cytotoxicity of topotecan by inhibiting autophagy in lung cancer cells显示文摘Although the anti-malaria drug chloroquine(CQ) has been shown to enhance chemotherapy and radiation sensitivity in clinical trials,the potential mechanisms underlying this enhancement are still unclear.Here,we examined the relevant mechanisms by which the multipotent CQ enhanced the cytotoxicity of topotecan(TPT).The lung cancer cell line A549 was treated with TPT alone or TPT combined with CQ at non-cytotoxic concentrations.Cell viability was assessed using the MTT assay.The percentage of apoptotic cells and the presence of a side population of cells were both determined by flow cytometry.Autophagy and the expression of Bcl-2 family proteins were examined by Western blotting.The accumulation of YFP-LC3 dots and the formation of acidic vesicular organelles were examined by confocal microscopy.CQ sensitized A549 cells to TPT and enhanced TPT-induced apoptosis in a Bcl-2 family protein-independent fashion.CQ inhibited TPT-induced autophagy,which modified the cytotoxicity of TPT.However,CQ failed to modify the transfer of TPT across the cytoplasmic membrane and did not increase lysosomal permeability.This study showed that CQ at non-cytotoxic concentrations potentiated the cytotoxicity of TPT by interfering with autophagy,implying that CQ has significant potential as a chemotherapeutic enhancer. | Yao Wang Rui-Qing Peng Dan-Dan Li Ya Ding Xiao-Qi Wu Yi-Xin Zeng Xiao-Feng Zhu Xiao-Shi Zhang | 2011 | Chinese Journal of Cancer2011，30，10： | 11 |
| 11 | Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma显示文摘包含蛋白质 1 的 Oxidored-nitro 领域(NOR1 ) 基因是首先从鼻咽的癌(NPC ) 孤立的新奇 nitroreductase 基因。它为它的 nitrosation 功能在化学致癌物的形成和 NPC 的 carcinogenesis 起一个重要作用。在鼻咽的癌细胞的野类型的 NOR1 基因的 Overexpression 是有效的禁止细胞生长和增长。在这研究,第一次,我们产生了高度特定的 NOR1 抗体并且在人的纸巾和 NPC 活体检视分析了 NOR1 分发。结果证明那 NOR1 蛋白质主要在人的鼻咽和气管的纸巾被表示。人的心,肝,怒气,胃,冒号,肾,骨胳的肌肉,胸腺,和胰都 NOR1 蛋白质是缺乏的。更重要地,我们在 NPC 纸巾执行了 NOR1 蛋白质表示的 immunohistochemistry 试金,并且结果证明那 NOR1 蛋白质在 NPC 经常是下面表示的。这些数据使 NOR1 的潜在的生理的函数的选择清楚些并且提供不可缺少的引用给 NPC 的 carcinogenesis 进程并且到识别或验证织物特定的药目标。 | Bo Xiang Mei Yi Li Wang Wei Liu Wenling Zhang Jue Ouyang Ya Peng Wenjuan Li Ming Zhou Huaying Liu Minghua Wu Rong Wan Xiaoling Li Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 10 |
| 12 | AduoLa Fuzhenglin Down-regulates Microwave-induced Expression of β_1-adrenergic Receptor and Muscarinic Type 2 Acetylcholine Receptor in Myocardial Cells of Rats显示文摘This paper is aimed to study the effect of ADL on expression ofβ1-AR and M2-AchR in myocardial cells of rats exposed to microwave radiation.Immunohistochemistry,Western blot and image analysis were used to detect the expression ofβ1-AR and M2-AchR in myocardial cells at 7 and 14 | ZHANG Jing PENG Rui Yun GAO Ya Bing WANG Shui Ming YANG Lei Lei ZHAO Li DONG Ji YAO Bin Wei CHANG Gong Min XIONG Lu | 2014 | Biomedical and Environmental Sciences2014，27，3： | 9 |
| 13 | Endovascular therapy for Acute ischemic Stroke Trial (EAST): study protocol for a prospective, multicentre control trial in China显示文摘Background and purpose:5 recent trials have shown the benefit of endovascular treatment for acute ischaemic stroke(AIS)due to large vessel occlusion of the anterior circulation.This study aims to evaluate the safety and efficacy of Solitaire thrombectomy in patients with moderate-to-severe stroke in the Chinese population,which has a high prevalence of intracranial atherosclerosis.Methods and analysis:This multicentre prospective control study will involve 17 stroke centres in China,and plans to recruit 150 patients in the intervention group,and 150 patients in the medical group,in which patients meet enrolment criteria but refuse intervention.Patients with AIS due to large vessel occlusion indicated for treatment with Solitaire stent retriever within 12 hours of symptom onset,and who meet the inclusion and exclusion criteria,will be enrolled in this study.The primary efficacy endpoint is functional independence as defined by a modified Rankin Scale(mRS)score≤2 at 90 days or by functional improvement as defined by mRS,using shift analysis.The procedural efficacy endpoint is arterial recanalisation of the occluded target vessel measured by a modified Thrombolysis in Cerebral Infarction(mTICI)score equal or superior to 2b right following the use of the study device.The primary safety endpoint is symptomatic intracranial haemorrhage(sICH)within 24±3 hours postprocedure.Ethics and dissemination:The protocol was approved by the Ethics Committee at the coordinating centre and by the local Institutional Review Board of each participating centre.Trial registration number:NCT02350283. | Zhongrong Miao Xiaochuan Huo Feng Gao Xiaoling Liao Chunjuan Wang Ya Peng Yibin Cao Shengli Chen Meng Zhang Changchun Jiang Xiaoxiang Peng Cunfeng Song Liping Wei Qiyi Zhu Zaiyu Guo Li Liu Hang Lin Hua Yang Wei Wu Hui Liang Anding Xu Kangning Chen Xingquan Zhao Yuesong Pan Hao Li Liping Liu Yilong Wang Yongjun Wang EAST investigators | 2016 | Stroke & Vascular Neurology2016，1，2： | 8 |
| 14 | The ploidy effects in plant gene expression: Progress, problems and prospects显示文摘Polyploidy and haploid are widely employed in the studies of genetics and evolution, and great pro-gress has been made in these fields, inspiring the enthusiasm of scientists to explore the ploidy effects in gene expression. In this paper, we review the gene expression and its regulation in polyploids, es-pecially in autopolyploids. We summarize some limitations in previous reports on polyploidy gene ex-pression and its regulation, especially the limitations in the research materials. We propose an idea to create homologous ploidy series with twin-seedlings and to employ high-throughput techniques to investigate the polyploidy transcriptome and its regulation. | PENG Hai1, ZHANG Jing1 & WU XianJun2,3 1 Laboratory of Germplasm and Genetics, College of Life Science, Jianghan University, Wuhan 430056, China 2 Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China 3 Key Laboratory of Southwest Crop Genetic Resources and Improvement of the Ministry of Education, Sichuan Agricultural Univer-sity, Ya‘an 620514, China | 2008 | Science China(Life Sciences)2008，51，4： | 8 |
| 15 | Timetable optimization for single bus line based on hybrid vehicle size model显示文摘This study proposes a flexible timetable optimization method based on hybrid vehicle size model to tackle the bus demand fluctuations in transit operation.Three different models for hybrid vehicle,large vehicle and small vehicle are built in this study,respectively.With the operation data of Shanghai Transit Route 55 at peak and off-peak hours,a heuristic algorithm was proposed to solve the problem.The results indicate that the hybrid vehicle size model excels the other two modes both in the total time and total cost.The study verifies the rationality of the strategy of hybrid vehicle size model and highlights the importance of the adaptive vehicle size in dealing with the bus demand fluctuation.The main innovation of the study is that unlike traditional timetables,the arrangement of the scheduling interval and the corresponding bus type or size are both involved in the timetable of hybrid vehicle size bus mode,which will be more effective to solve the problem of passenger demand fluctuation.Findings from this research would provide a new perspective to improve the level of regular bus service. | Daniel(Jian) Sun Ya Xu Zhong-Ren Peng | 2015 | Journal of Traffic and Transportation Engineering(English Edition)2015，2，3： | 8 |
| 16 | NGX6 inhibits AP-1 and Ets-1 expression and down-regulates cyclin D1 in human coloreetal cancer显示文摘Colorectal 癌症(CRC ) 是与病态和死亡的增加的发生被联系的一个普通恶意的肿瘤。鼻咽的联系癌的基因(NGX6 ) 6 是肿瘤转移的新奇候选人 suppressor 基因,它在 CRC 是下面调整的。在现在的学习,我们构造了 colorectal 织物 microarray 在 CRC 纸巾检验 NGX6, phospho-c-Jun N 终端 kinase (p-JNK ) ,和 phospho 细胞外的调整信号的 kinase (津贴) 的表示侧面。而 p-JNK 和津贴的表情在 CRC 纸巾是更高的,我们发现 NGX6 表示在 CRC 纸巾和变形淋巴节点是更低的,比在正常肠的 mucosa。NGX6, p-JNK,和津贴的表情与 colorectal 纸巾的临床的病理学的特征被联系。NGX6 overexpression 禁止了激活和 JNK1 的原子 translocation,它在细胞质导致了 p-JNK 的累积,但是没禁止激活和 ERK1/2 的原子 translocation。NGX6 也禁止了抄写因素 AP-1 (c-jun 和 c-fos ) 和 Ets-1 的表示。另外, NGX6 overexpression 减少了 cyclin D1 并且戏剧性地的表示压制了 cyclin D1 倡导者的 transcriptional 效率。我们建议那 NGX6 表情沉醉于人的 colorectal carcinogenesis 的多步过程。它的 overexpression 能禁止抄写因素的表示 AP-1 和 Ets-1,和下面调整在人的 CRC 的 cyclin D1 倡导者的 transcriptional 活动。 | Ya Peng Hongtao Li Minghua Wu Xiaoyan Wang Songqing Fan Fen Liu Bo Xiang Qin Guo Xueyuan Tang Shourong Shen | 2009 | Acta Biochimica et Biophysica Sinica2009，41，6： | 8 |
| 17 | Cultured Mycelium Cordyceps sinensis alleviates CCI4-induced liver inflammation and fibrosis in mice by activating hepatic natural killer cells显示文摘目的: 最近的证据证明有教养的菌丝体 Cordyceps sinensis (CMCS ) 有效地在老鼠免于肝纤维变性。这里,我们调查了 CMCS 的 anti-fibrotic 行动是否与它肝的生来的杀手( NK )的活动的规定有关是在 CCl 4-treated mice.Methods 的房间: C57BL/6 老鼠与10% CCl 4被注射( 2 mL/kg , ip ) 3 次每为 4 个星期的星期,并且收到了 CMCS ( 120 mg· ; kg −1·d−1, ig )在这个时期期间。在实验的另一部分,老鼠也与 NK 删除房间的抗体 ASGM-1 被注射(20 μ g, ip ) 在开始的 3 个星期内的 5 次。在老鼠被牺牲以后,浆液肝功能,和肝发炎, hydroxyproline 内容和骨胶原免职被估计。肝的 NK 房间和孤立的肝淋巴细胞上的 NKG2D (NK 房间的激活受体) 的表示的数字用流动 cytometry 被分析。在肝纸巾的 Desmin 表示和房间 apoptosis 分别地用染色的 desmin 和 TUNEL 试金被学习。α 的层次; -SMA, TGF-β, RAE-1δ并且 RAE-1ε在肝,纸巾被 RT-qPCR.Results 决定: 在 CCl 4-treated 鼠标, CMCS 管理显著地改进了肝功能,稀释的肝发炎和纤维变性,并且在肝的 NK 房间上增加了肝的 NK 房间的数字和 NKG2D 的表达式水平。而且, CMCS 管理显著地减少了在肝纸巾的 desmin 表示,和邻近肝的星形的房间的染色的增加的 TUNEL。有 NK 删除房间的 ASGM-1 的注射不仅减少在 CCl 4-treated 老鼠的肝的 NK 房间的数字,而且极大地加速的肝发炎和纤维变性。在有 NK 房间弄空的 CCl 4-treated 老鼠, CMCS 管理速度肝纤维变性开发的率,并且温和地 upregulated 肝的 NK 房间,但是没有改变 NKG2D expression.Conclusion 的数字: CMCS allevi¬经由支持肝的 NK 房间的激活的 ates CCl 4-induced 肝发炎和纤维变性。CMCS 部分颠倒肝的 NK 房间的 ASGM-1-induced 弄空。 | Yuan PENG Kai HUANG Li SHEN Ya n-ya n TAO Cheng-hai LIU | 2016 | Acta Pharmacologica Sinica2016，37，2： | 8 |
| 18 | Behavioral Abnormality along with NMDAR-related CREB Suppression in Rat Hippocampus after Shortwave Exposure显示文摘Objective To estimate the detrimental effects of shortwave exposure on rat hippocampal structure and function and explore the underlying mechanisms. Methods One hundred Wistar rats were randomly divided into four groups(25 rats per group) and exposed to 27 MHz continuous shortwave at a power density of 5, 10, or 30 m W/cm^2 for 6 min once only or underwent sham exposure for the control. The spatial learning and memory, electroencephalogram(EEG), hippocampal structure and Nissl bodies were analysed. Furthermore, the expressions of N-methyl-D-aspartate receptor(NMDAR) subunits(NR1, NR2 A, and NR2 B), c AMP responsive element-binding protein(CREB) and phosphorylated CREB(p-CREB) in hippocampal tissue were analysed on 1, 7, and 14 days after exposure. Results The rats in the 10 and 30 m W/cm^2 groups had poor learning and memory, disrupted EEG oscillations, and injured hippocampal structures, including hippocampal neurons degeneration, mitochondria cavitation and blood capillaries swelling. The Nissl body content was also reduced in the exposure groups. Moreover, the hippocampal tissue in the 30 m W/cm^2 group had increased expressions of NR2 A and NR2 B and decreased levels of CREB and p-CREB. Conclusion Shortwave exposure(27 MHz, with an average power density of 10 and 30 m W/cm^2) impaired rats' spatial learning and memory and caused a series of dose-dependent pathophysiological changes. Moreover, NMDAR-related CREB pathway suppression might be involved in shortwave-induced structural and functional impairments in the rat hippocampus. | YU Chao BAI Yan Xin XU Xin Ping GAO Ya Bing HAO Yan Hui WANG Hui TAN Sheng Zhi LI Wen Chao ZHANG Jing YAO Bin Wei DONG Ji ZHAO Li PENG Rui Yun | 2019 | Biomedical and Environmental Sciences2019，32，3： | 7 |
| 19 | Three-dimensional image of hepatocellular carcinoma under confocal laser scanning microscope显示文摘AIM To investigate the application of confocallaser scanning microscopy(CLSM)in tumorpathology and three-dimensional( 3-D )reconstruction by CLSM in pathologic specimensof hepatocellular carcinoma(HCC).METHODS The 30μm thick sections were cutfrom the paraffin-embedded tissues of HCC,hyperplasia and normal liver,stained with DNAfluorescent probe YOYO-1 iodide and examinedby CLSM to collect optical sections of nuclei and3-D images reconstructed.RESULTS HCC displayed chaotic arrangementof carcinoma cell nuclei,marked pleomorphism,indented and irregular nuclear surface,andirregular and coarse chromatin texture.CONCLUSION The serial optical tomograms ofCLSM can be used to create 3-D reconstruction ofcancer cell nuclei.Such 3-D impressions mightbe helpful or even essential in making anaccurate diagnosis. | Wang Hai Zhang Shi Neng Zhu Shi Lun Lu Ya Lin Huang Peng Zhao | 2000 | World Journal of Gastroenterology2000，6，3： | 7 |
| 20 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
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