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| 1 | Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro显示文摘Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods:Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymis in modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS and incubated with methoxychlor (1 μnol/L, 10 μmol/L and 100 μmol/L) and methoxychlor + vitamin C (100μmol/L each) for 3 h at 32℃. After incubation, the sperm motility and viability were assessed. An aliquot of sperm sample was homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione reductase and lipid peroxidation. Results: In methoxychlor-incubated sperm and in sperm co-incubated with methoxychlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the corresponding controls. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of sperm with methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase and in the level of lipid peroxidation. Conclusion: Methoxychlor induced oxidative stress in epididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlor and vitamin C, a natural antioxidant, reversed the effect of methoxychlor. | Bindu Gangadharan M.Arul Murugan P.P.Mathur | 2001 | Asian Journal of Andrology2001，3，4： | 13 |
| 2 | Effect of lindane on testicular antioxidant system and steroidogenic enzymes in adult rats显示文摘Aim: To find out the effect of lindane on testicular antioxidant system and testicular steroidogenesis in adult male rats. Methods: Adult male rats were orally administered with lindane at a dose of 5.0 mg/kg body weight per day for 30 days. Twenty-four hours after the last treatment the rats were killed using anesthetic ether. Testes, epididymis,seminal vesicles and ventral prostate were removed and weighed. A 10% testicular homogenate was prepared and cen trifuged at 4°C. The supematant was used for various biochemical estimations. Results: The body weight and the weights of testes, epididymis, seminal vesicles and ventral prostate were reduced in lindane-treated rars. There was asignificant decline in the activities of antioxidant enzymes superoxide dismutase (SOD), catalase and glutathione reduc tase while an increase in hydrogen peroxide (H2O2) generation was observed. The specific activities of testicular steroidogenic enzymes 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase were decreased. The levels of DNA, RNA and protein were also decreased in lindane-treated rats. Conclusion: Lindane induces oxida tive stress and decreases antioxidant enzymes in adult male rats. | R.Sujatha K.C.Chitra C.Latchoumycandane P.P.Mathur | 2001 | Asian Journal of Andrology2001，3，2： | 7 |
| 3 | Effect of lindane on antioxidant enzymes in epididymis and epididymal sperm of adult rats显示文摘Aim: To find out the changes induced by lindane on the antioxidant enzymes in epididymis and epididymal sperm of adult rats. Methods: Adult male rats were orally administered lindane at a dose of 5.0 mg/kg body weight per day for 30 days. At the end of the treatment, the rats were sacrificed. The epididymis was removed and weighed and sperm were collected for sperm count, motility and biochemical studies. A 1% homogenate of epididymis was prepared and used for biochemical estimations. Results: In lindane-treated rats, there were significant reductions in the epididymal weight, epididymal sperm count and motility compared with the controls. Significant decreases in the superoxide dismutase (SOD), catalase, glutathione reductase and glutathione peroxidase activities and significant increases in the H2O2 generation and lipid peroxidation were also observed in the epididymis and epididymal sperm of lindane-treated rats. Conclusion: Lindane decreases the levels of antioxidant enzymes in the epididymis and epididymal sperm of adult rats thereby inducing oxidative stress. | K.C.Chitra R.Sujatha C.Latchoumycandane P.P.Mathur | 2001 | Asian Journal of Andrology2001，3，3： | 6 |
| 4 | Chronic effect of endosulfan on the testicular functions of rat显示文摘Aim: To find out the toxic effect of endosulfan on the tesficular function of pubertal rats, Methods: Male rats of pu-bertal age were orally administered endosulfan at a dose of 1.0 mg/kg body weight for 30 days. Twenty-four hours af-ter the last tmagnent, the rats were sacrificed and the testis, epididymis, seminal vesicles and ventral prostate were re-moved and weighed. A 10 % testicular homogenate was prepared for biochemical estimations. Results: In endosul-fan-treated rats, there were a reduction in the body weight and the weights of testis and accessory sex organs, a de-crease in the testicular lactate and pyruvate activities, and in the testicular DNA and RNA concentrations, whereas thetesticular protein concentration was slightly increased; the specific activity of testicular steroidogenic enzyme, 33OH-steroid dehydrogenase and the ascorbic acid level were decreased, which were correlated with a decrease in steroidoge-nesis. The lysosomal enzyme acid phosphatase and brush-border enzyme alkaline phosphatase activities were also de-creased in the testis of treated rats. Conclusion: In puhertal rats, endosulfan treaanent inhibits the testicular functions.(Asian J Androl 1999 Dec; 1 : 203 - 206) | K.C.Chitra C.Latchoumycandane P.P.Mathur | 1999 | Asian Journal of Andrology1999，1，4： | 5 |
| 5 | Effect of bisphenol A and co-administration of bisphenol Aand vitamin C on epididymis of adult rats:A histological and biochemical study显示文摘Aim: To study the effect of bisphenol A on the epididymis and epididymal sperm of rats and the possible amelioration action of co-administration with vitamin C. Methods: Male Wistar rats were orally administered bisphenol A (0.2μg·kg^-1,2μg·kg^-1·day^-1 and 20μg.kg^-l.day^-1)and 0.2μg ,2μg and 20μg bisphenol A + 40 mg vitamin C·kg^-1·day^-1 for 60 days. On day 61, rats were killed with anesthetic ether and sperm collected from epididymis were used for assessment of sperm count, motility and viability and biochemical studies. A 1% homogenate of epididymis was prepared and used for biochemical estimations. Caput, corpus and cauda epididymis were fixed in Bouin's fixative for histological studies. Results: Administration of bisphenol A caused a reduction in the epididymal sperm motility and count and the sperm viability remained unchanged. The activities of superoxide dismutase and glu-tathione peroxidase decreased, while the levels of lipid peroxidation increased in epididymal sperm and epididymis atall doses. Co-administration with vitamin C reversed the effect of bisphenol A-induced oxidative stress in epididymal sperm and epididymis. A complete degeneration of epididymal epithelium in caput, corpus and canda regions with reduction in the number of sperms were observed at all doses of bisphenol A-treated rats. Conclusion: Bisphenol A induced oxidative stress in epididymis and caused degeneration of the epididymal epithelium of rats. Co-administra-tion with vitamin C had a protective effect against the bisphenol A-induced toxicity in epididymal sperm and epididymis.( Asian J Androl 2003 Sep. 5: 203-208) | K.C.Chitra K.Ramachandra Rao P.P.Mathur | 2003 | Asian Journal of Andrology2003，5，3： | 3 |
| 6 | Effect of piperine on the epididymis of adult male rats显示文摘瞄准:在成年雄的老鼠的附睾抗氧化剂系统上学习胡椒碱的效果。方法:成年雄的老鼠每天是在 1 mg/kg, 10 mg/kg 和 100 mg/kg 体重的剂量的口头上地管理的胡椒碱 30 连续的天了。在最后治疗以后的 24 个小时,老鼠与醚和附睾被称并且杀死从身体被把。从附睾的尾区域收集的精子被用于对它的计数,活动性和生存能力的评价。头,语料库和附睾的尾区域被分开并且独立一致获得 10 % homogenates。上层清液被用于涎的的酸的、超级氧化物歧化酶的试金,过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽还原酶,类脂化合物每氧化和过氧化氢产生。结果:对待胡椒碱的老鼠的体重仍然保持未改变。头,语料库和附睾的尾区域的重量显著地在 100 mg/kg 的剂量减少了。附睾精子计数和活动性在 10 mg/kg 和 100 mg/kg 减少了,并且精子生存能力在 100 mg/kg 显著地减少了。当在尾区域的重要减少独自在 10 mg/kg 被观察时,在附睾的涎的酸层次在 100 mg/kg 显著地减少了。在超级氧化物歧化酶,过氧化氢酶,谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活动的重要衰落,与过氧化氢的增加一起,产生和类脂化合物在 10 mg/kg 和 100 mg/kg 每氧化被观察。结论:在抗氧化剂酶和涎的酸的活动引起减少的胡椒碱在附睾和能损坏附睾环境和精子功能的从而增加的反应的氧种类层次铺平。 | S.C.D'cruz P.P.Mathur | 2005 | Asian Journal of Andrology2005，7，4： | 2 |
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