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| 1 | Strategies for production of active eukaryotic proteins in bacterial expression system显示文摘Bacteria have long been the favorite expression system for recombinant protein production.However,the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias,protein folding,phosphorylation,glycosylation,mRNA stability and promoter strength.Factors are cited and the methods to convert to soluble and active proteins are described,for example a tiglit control of Escherichia coli milieu,refolding from inclusion body and through fusion technology. | Orawan Khow Sunutcha Suntrarachun | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，2： | 8 |
| 2 | 固定化青霉素酰化酶在光敏感可再生两水相体系中催化青霉素G为6-APA显示文摘在一种光敏感可再生高聚物(PNBC 300000)与葡聚糖20000(Dextran 20000)形成的两水相体系中进行固定化青霉素酰化酶的相转移催化青霉素G产生6-APA的反应。在这个两水相体系中,当pH为7.8,底物浓度为62 mmol/L,反应温度为20℃,在50 mmol/L KCl存在下,6-APA的分配系数可达8.4。催化动力学显示,达到平衡的时间近6 h,PG(Na)转化率约82.6%。3批半连续反应转化率为60%~70%,较相近条件下的单水相反应得率提高近20%。在两水相中,底物及产物主要分配在上相,固定化酶分配在下相,底物青霉素G进入下相经酶催化产生的6-APA及苯乙酸又转入上相,从而解除了青霉素酰化酶催化反应的底物及产物抑制作用,达到提高产物得率的效果。形成两水相的高聚物通过488 nm的激光照射可实现循环利用,高聚物的光照回收率在95%~98%。 | 金科铭 曹学君 庄英萍 储炬 张嗣良 Orawan Duerasor | 2007 | 华东理工大学学报(自然科学版)2007，33，3： | 2 |
| 3 | Evaluation of yield and nutritive value of kenaf (Hibiscus eannabinus) at various stages of maturity 显示文摘 | Anut Chantiratikul Chakrapong Chaikong Orawan Chinrasri | 2009 | Pakistan Journal of Nutrition2009，8，7： | 1 |
| 4 | Early neurodevelopment of infants born to HIV-seropositive mothers 显示文摘 | Orawan L | 2004 | Chiang Mai Med Bull2004，43，1： | 1 |
| 5 | New Thai herbal shampoos as pediculicides for killing head louse, Pediculus humanus capitis De Geer(Phthiraptera)显示文摘Objective: To evaluate the efficacy of Thai herbal shampoos [Cartoxylum formosum(C.formosum) + eucalyptus essential oil(EO), C. formosum + citrus EO, Solanum trilobatum+ eucalyptus EO, Solanum trilobatum + citrus EO, Moringa oleifera + eucalyptus EO and Moringa oleifera + citrus EO] for killing all stages of Pediculus humanus capitis(Phthiraptera).Methods: A filter paper contact method was applied with three concentrations(0.05, 0.10 and 0.20 mL/cm^2) of each Thai herbal shampoo as well as permethrin pediculicide(positive control) and drinking water(negative control) against eggs, nymphs and adults of Pediculus humanus capitis. Mortality rates of the eggs were recorded after 7 days of incubation while those of nymphs and adults were recorded after 5 minutes of contact. Results: All herbal shampoos at the high concentration were highly effective against nymphs and adults, but not effective against the eggs. C. formosum + eucalyptus EO and C. formosum + citrus EO shampoos at all concentrations exhibited the highest efficacy against nymphs and adults with100% mortality rate at 5 min and LC_(50) values of 0.004 and 0.005 mL/cm^2, respectively. All formulation of Solanum trilobatum and Moringa oleifera shampoos added with eucalyptus EO showed mortality rates against nymphs at 92.0%-100.0% and 76.0%-100.0% and against adults at 84.0%-100.0% and 20.0%-32.0%, respectively. Permethrin pediculicide was not effective against the eggs, but showed 68.0%-92.0% and 28.0%-60.0% mortality rates against nymphs and adults. Conclusions: These results indicate that C. formosum + eucalyptus EO shampoo can be used as an effective nymphicide and adulticide against Pediculus humanus capitis. | Sirawut Sittichok Orawan Wongnet Mayura Soonwera | 2018 | Asian Pacific Journal of Tropical Biomedicine2018，8，2： | 1 |
| 6 | Silanerubber coupling in sulfure, peroxide and metal oxide curing systems 显示文摘 | Orawan Taikum Hans-Detlef Luginsland | 2004 | Rubber World2004，230，5： | 1 |
| 7 | Physicochemical properties of extrusion-modified konjac glucomannan显示文摘 | ORAWAN T SANGGUANSRI C WILLIAM L K | 2012 | Carbohydrate Polymers2012，87，： | 1 |
| 8 | In vitro biological evaluation of electrospun cellulose acetate fiber mats containing asiaticoside or curcumin显示文摘 | Orawan Suwantong Uracha Ruktanonchai Pitt Supaphol | | 0，，04： | 1 |
| 9 | Electrospun cellulose acetate fiber mats containing asiaticoside or Centella asiatica crude extract and the release characteristics of asiaticoside显示文摘 | Orawan Suwantong Uracha Ruktanonchai Pitt Supaphol | | 0，，19： | 1 |
| 10 | Conducting polymer nanoparticles synthesized in an liquid by chemical polymerisation显示文摘 | Jennifer M Pringle Orawan Ngamna Chen Jun | 2006 | Synthetic Metals2006，156，： | 1 |
| 11 | Lupus nephritis and Raynaud’s phenomenon are significant risk factors for vascular thrombosis in SLE patients with positive antiphospholipid antibodies显示文摘 | Kittiwan Choojitarom Orawan Verasertniyom Kitti Totemchokchyakarn Kanokrat Nantiruj Vasant Sumethkul Suchela Janwityanujit | 2008 | Clinical Rheumatology2008，，3： | 1 |
| 12 | Genetic dissection of carotenoids in maize kernels using high-density single nucleotide polymorphism markers in a recombinant inbred line population显示文摘Carotenoids are antioxidants and vitamin A precursors that have important roles in human health. Hence, improving the carotenoid contents in maize kernels is a priority objective for breeders in order to obtain nutritional biofortification outcomes. In the current study, the genetic architecture of carotenoids in maize kernels was explored using a recombinant inbred line(RIL) population derived from a cross between inbred lines By804 and B73. A total of 81 QTLs were detected by using a high-density bin map and a simple sequence repeat(SSR)-based linkage map, with one to seven QTLs for each trait explaining 4.21%–47.53% of the phenotypic variation. A comparison of the QTL mapping efficiency between the two linkage maps revealed that the high-density bin map had higher resolution. In the current study 46 additional QTLs were identified, with 16 being common with previous studies and14 newly identified. Among the results, 29.6%(24/81) of QTLs explained > 10% of the phenotypic variation in the RIL population, and 70.4%(57/81) explained ≤ 10%. These results suggest that a few large-effect QTLs, together with a variable number of minor-effect QTLs,contributed to most of the genetic components of carotenoids in maize kernels. | Orawan Jittham Xiuyi Fu Jing Xu Subhash Chander Jiansheng Li Xiaohong Yang | 2017 | The Crop Journal2017，5，1： | 1 |
| 13 | Simultaneous determina- tion of some food additives in soft drinks and other liquid foods by flow injection on-line dialysis coupled to high performance liquid chromatography显示文摘 | Kritsunankul Orawan Jakmunee Jaroon | 2011 | Talanta2011，84，5： | 1 |
| 14 | Early neurodevelopment of infants born to HIV-sero- positive mothers显示文摘 | Orawan L | 2004 | Chiang Mai Med Bull2004，43，1： | 1 |
| 15 | Effect of phosphate compounds on gel-forming ability of surimi from bigeye snapper (Priacanthus tayenus) 显示文摘 | Orawan J Soottawat B Wonnop V | 2006 | Food Hydrocolloids2006，20，8： | 1 |
| 16 | Effect of phosphate compounds on gel-forming ability of surimi from bigeye snapper (Priacanthus tayenus)显示文摘 | Orawan Julavittayanukul Soottawat Benjakul Wonnop Visessanguan | | 0，，20： | 1 |
| 17 | Flow injection on-line dialysis coupled to high performance liquid chromatography for the determination of some organic acids in wine显示文摘 | Orawan Kritsunankul Benjapom Pramote Jaroon Jakmunee | 2009 | Talanta2009，，： | 1 |
| 18 | Use of centrifugation-filtration for determination of syneresis in freeze-thaw starch gels显示文摘 | Sanguansri Charoenrein Orawan Tatirat Janya Muadklay | 2008 | Carbohydr Polymer2008，73，1： | 1 |
| 19 | High Rates of Oxygen Reduction over a Vapor Phase- Polymerized PEDOT Electrode显示文摘 | Winther-Jensen Bjorn Winther-Jensen Orawan Forsyth Maria | 2008 | Science2008，321，： | 1 |
| 20 | Early neurodevelopment of infants born to HIV-seropositive mothers显示文摘 | Orawan L | 2004 | Chiang Mai Med Bull2004，43，1： | 1 |
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