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| 1 | Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis显示文摘Semen analysis has long been used to evaluate male fertility.Recently,several sperm function tests have been developed.Of those,the sperm DNA fragmentation index(DFI),which describes the status of the sperm DNA,is thought to be a suitable parameter for evaluating male fertility.However,there have been no large-scale studies on the sperm DFI of Japanese men.Therefore,we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay(SCSA)to assess male fertility in Japan.This study enrolled 743 infertile and 20 fertile Japanese men.To evaluate reproducibility,inter-and intraobserver precision was analyzed.A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine insemination.The variability of the sperm DFI among fertile volunteers was determined.The relationship between semen parameters and the sperm DFI was assessed by Spearman’s rho test.A precision analysis revealed good reproducibility of the sperm DFI.The cutoff value of sperm DNA fragmentation in infertile men was 24.0%.Semen volume had no relationship with the sperm DFI.Sperm concentration,sperm motility,total motile sperm count,and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm DFI.The median sperm DFI was smaller in fertile volunteers(7.7%)than that in infertile men(19.4%).Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis. | Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto | 2022 | Asian Journal of Andrology2022，24，1： | 2 |
| 2 | Impact of weather factors on Mycoplasma pneumoniae pneumoniae显示文摘 | Onozuka D Hashizume M Hagihara A | | 0，，： | 1 |
| 3 | Identification of dynamic cutting force coefficients and chatter stability with process damping显示文摘 | Altintas Y Eynian M Onozuka H | | 0，，： | 1 |
| 4 | The influence of temperature and humidity on the incidence of hand,foot,and mouth disease in Japan显示文摘 | Onozuka D Hashizume M | 2011 | Sci Total Env2011，410，411： | 1 |
| 5 | Mutational analysis of the ATP2A2 gene in two Dafter disease families with intrafamilial variability 显示文摘 | Onozuka T Sawamura D Yokota K | 2004 | Br J Dermatol2004，150，4： | 1 |
| 6 | Characteriza- tion of Magnaporthe oryzae Chrysovirus 1 structural proteins and their expression in Saccharomyces cerevi- siae 显示文摘 | Urayama S Ohta T Onozuka N | 2012 | Journal of Virology2012，86，15： | 1 |
| 7 | Low-activation Mn-Cr austenitic stainless with further reduced content of long-lived radioactive elements 显示文摘 | Masanori Onozuka Tomikane Saida Shouzou Hiral et ol | 1998 | Journal of Nuclear Materials1998，255，23： | 1 |
| 8 | Nobiletin,a citrus flavonoid,improves memory impairment and A ppathology in a transgenic mouse model of Alzheimer's disease显示文摘 | Onozuka H Nakajima A Matsuzaki K | 2008 | J Pharmacol Exp Ther2008，326，3： | 1 |
| 9 | Four subtypes ofpetroclival meningiomas:differences in symptoms andoperative findings using the anterior transpetrosal approach显示文摘 | Ichimura S Kawase T Onozuka S | 2008 | Acta Neurochir(Wien)2008，150，7： | 1 |
| 10 | A novel diagnostic method for human immunodeficiency virus type-1 in plasma by near-infrared spectroscopy显示文摘 | Sakudo A Tsenkova R Onozuka T | 2005 | Microbiol Immunol2005，49，7： | 1 |
| 11 | Non-destructive testing of bonded structures for plasma facing components 显示文摘 | Onozuka M Kikuchi K Kirihigashi A | 2005 | Fusion Engineering and Design2005，7579，： | 1 |
| 12 | Identification of dynamic cutting force coefficients and chatter stability with process damping 显示文摘 | Altintas Y Eynian M Onozuka H | 2008 | CIRP Annals Manufacturing Technology2008，57，1： | 1 |
| 13 | Clinical significance of anti-centromere antibody and anti-CENP B antibody in sera of patients with primary biliary cirrhosis 显示文摘 | Onozuka Y Shibata M Yonezawa H | 1996 | Rinsho Byori1996，44，9： | 1 |
| 14 | Electrospinning processed nanofibmus TiO2 membranes for photoboltaic applications显示文摘 | Katsuhiro Onozuka Bin Ding Yosuke Tsuge Takayuki Naka Michiyo Yamazaki | 2006 | Nanotechnology2006，17，： | 1 |
| 15 | The influence of temperature and humidity on the incidence of hand,foot,and mouth disease in Japan显示文摘 | Onozuka D Hashizume M | 2011 | SciTotal Environ2011，，： | 1 |
| 16 | Nasal and nasal-type T/ NK-cell lymphoma with cutaneous involvement 显示文摘 | Kato N Yasukawa K Onozuka T | 1999 | J Am Acad Dermatol1999，40，52： | 1 |
| 17 | Paraneoplastic syndromes of leukocytosis, thrombocytosis, and hypercalcemia associated with squamous cell carcinoma 显示文摘 | Kato N Yasukawa K Onozuka T | 1999 | J Dermatol1999，26，6： | 1 |
| 18 | Four subtypes of petroclival meningiomas: differences in symptoms and operative findings using the anterior transpetrosal approach 显示文摘 | Ichimura S Kawase T Onozuka S | 2008 | Acta Neurochir ( Wien)2008，150，7： | 1 |
| 19 | Identification of dynamic cutting force coefficients and chatter stability with process damping 显示文摘 | Altintas Y Eynian M Onozuka H | 2008 | CIRP Annals-manufacturing Technology2008，57，1： | 1 |
| 20 | Perovskite-type L%Ti207 Mesoporous Photocatalyst 显示文摘 | Onozuka K Kawakami Y hnai H | 2012 | J Solid State Chem2012，192，： | 1 |
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