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| 1 | Dynamic changes of typeⅠ,Ⅲand N collagen synthesis and distribution of collagen-producing cells in carbon tetrachloride-induced rat liver fibrosis显示文摘AIM To find out the relationship between the gene transcription of different types ofprocollagen and the deposition of the relevant collagens in the liver tissue and to confirm the types of collagen producing cells in liverfibrogenesis.METHODS Dynamic changes of the expression of α1(Ⅰ ), α1 (Ⅲ ) and α1 (Ⅳ) procollagen mRNAand relevant collagens and the distribution ofcollagen producing cells during liver fibrogenesis of rat induced by CCl4 (20 weeks)were investigated with Northern blot analysis,in situ hybridization and immunohistochemicaltechniques.RESULTS The increased expression of α1 (Ⅲ)procollagen mRNA by Northern blot analysis was the most predominant one among the threemRNAs during fibrogenesis. However, theenhanced expression of al (Ⅳ) procollagenmRNA occurred very early while the expressionof α1 (Ⅰ) mRNA was not enhanced much until themiddle stage of the exPeriment. D6smin (Dm)positive hepatic stellate cells (HSCs) and fewmyofibroblasts (MFs) in and around the necrotic areas expressed α1 (Ⅰ), α1 (Ⅲ) and α1 (Ⅳ)procollagen mRNA signals detected by in situhybridization at the early stage of theexperiment. All the three procollagen mRNAsignals thereafter mainly localized in fibroblasts (Fbs) and MFs in fibrotic septa during the middle and late stages of fibrosis, which distributedparallel to the corresponding collagens detected by immunohistochemical study. ln addition, the endothelial cells of sinusoids and the small blood vessels within the septa also showed α1(Ⅳ) procollagen mRNA and type Ⅳ collagen expressionCONCLUSION It is considered that 'HSC-MF-Fb' effect cell system is the major cellularsource of collagen production in liver fibrosis, in which HSCs are collagen producing precursor cells in the early liver fibrogenesis, thereafter the synthesis of type Ⅰ, Ⅲ and Ⅳ collagens (Col Ⅰ, Col Ⅲ and Col Ⅳ) mainly derives fromMFs and Fbs, which play a very important role in the progress of liver fibrosis. The endothelialcells along sinusoids, as another source of Col Ⅳ production, might participate in the capillization of liver sinusoids. | DU Wei-Dong ZHANG Yue-E ZHAl Wei-Rong and ZHOU Xiao-Mei(Department of Pathology, Shanghai Medical University, Shanghai200032, China)(National Laboratory for Oncogenes and Related Genes, ShanghaiCencer Institute)See invited commentary on page 388 | 1999 | World Journal of Gastroenterology1999，5，5： | 47 |
| 2 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 3 | The tumor-selective over-expression of the human Hsp 70 gene is attributed to the aberrant controls at both initiation and elongation levels of transcription显示文摘The tumor selective over-expression of the human Hsp70 gene has been well documented in human tumors,linked to the poor prognosis,being refractory to chemo-and radio-therapies as well as the advanced stage of tumorous lesions in particular.However,both the nature and details of aberrations in the control of the Hsp70 expression in tumor remain enigmatic.By comparing various upstream segments of the Hsp70 gene for each''s ability to drive the luciferase reporter genes in the context of the tumor cell lines varying in their p53 status and an immortal normal liver cell line,we demonstrated in a great detail the defects in the control mechanisms at the both initiation and elongation levels of transcription being instrumental to the tumor selective profile of its expression.Our data should not only offer new insights into our understanding of the tumor specific over-expression of the human Hsp70 gene,but also paved the way for the rational utilization of the tumor selective mechanism with the Hsp70 at the central stage fortargeting the therapeutic gene expression to human tumors. | LING CAI, JING DE ZHU,The State-key Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, LN 25/2200, Xie-tu Road, Shanghai 200032, China | 2003 | Cell Research2003，13，2： | 13 |
| 4 | The promoter analysis of the human C17orf25 gene, a novel chromosome 17pl3.3 gene显示文摘The human C17orf25 gene (Accession No. AF177342) is one of thirteen genes cloned from a region displaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular car-cinoma in China[l]. To unveil the underlying mechanisms for the transcription regulation of this gene and understand its implication to the hepatocellular carcinogenesis, we looked into the relevant aspects by both bioinformatic and experimental executions. We found: 1, The abundant expression of the C17orf25 gene was evident in all the cell lines and tissue samples tested, showing little hepatoma-selectivity; 2, Its tran-scription starts at a single site, locating at -60 from the translation initiation codon; 3, A 58 bp fragment containing the transcription start, extending from -112 to -55, represents the minimal promoter; 4, The consensus sequence within this fragment recognized by SP1 contributes predominantly to the activity of the minimal promoter; 5, The bioinformatic analysis suggests that the C17ort25 gene may encode a protein in the family of the glyoxalase. Our data has provided some deep insight into both function and regulation of the C17orf25 gene in the context of the normal liver and hepatocellular carcinoma. | JIAN YING GUO, JIAN XU, DA QIN MAO, LI LI FU, JIAN REN GU, JING DE ZHUThe State-Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ln 2200/25, Xie-Tu Road, Shanghai 200032, China | 2002 | Cell Research2002，12，5： | 7 |
| 5 | Ge- HPV and gain of the human te- Iomerase gene TERC at 3q26 are strongly associated events in the progression of uterine cervical dysp lasia to invasive cancer显示文摘 | AHN Hoprrlan W Theelen nomic integration of oncogenic PPH Hommelberg | 2006 | J Palhl2006，210，4： | 1 |
| 6 | Drug -induced expression of the cellular adhesion molecule LI CAM con-fers anti - apoptotic protection and chemoresistance in pancreaticductal adenocarcinoma cells显示文摘 | Sebens Mtierk6ster S Werbing V Sipos B Oncogene | 2007 | Cancer2007，26，19： | 1 |
| 7 | Distinct pools ike cells coexist within hum&n glioblastomas &nd tumorigenicity &nd independent genomic evolution显示文摘 | Picciri of cancer stem- display different Oncogene 2009 28( Io SG Combi g Cajol& L et aI | 2009 | 5)2009，，： | 1 |
| 8 | Muhidrug resistance mediated by the breast cancer resistance protein BCRP (ABCG2) 显示文摘 | Austin D Douglas DR Oncogene | 2003 | Basingstoke2003，22，47： | 1 |
| 9 | The value of plants used in traditional medicine for drug discorvery显示文摘 | Human telomere oncogenic promoter diverse higher order DNA and RNA Nucleic Acids Res 2007 35(22) : Fabricant DS Farnsworth NR | 2001 | Environ Health Perspect2001，109，1： | 1 |
| 10 | A clinico-pathologic study of 12cases显示文摘 | Colorectal neuroendocrine carcinomas and adenocarcinomas share oncogenic pathways | 2012 | European journal of gastroenterology and hepatology2012，24，12： | 1 |
| 11 | Oncogenic c-Ki- c c-Ha-ras CEA expression and disrupts baso in colon epithelial cells显示文摘 | YAN Z DENG X CHEN ras but not oncogen M | 1997 | J Biol up-regulates ateral polarity Chem1997，272，27： | 1 |
| 12 | Affinity chromatography-dependent selection (ACDS)of genomic DNA fragments bound specifically to bacterial synthesized Myc/Myn proteins显示文摘This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in E.coli hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed. | SHI CAN PEI WANG YONGJUN HU LIAN XU. (Oncogene Group, Laboratory of Molecular and Cellular Oncology, Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China) | 1995 | Cell Research1995，5，1： | 0 |
| 13 | The inhibitory effect of transthyretin gene on growth of human hepatoma cells显示文摘Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer. Its mRNA expression was suppressed in most hepatoma samples. In order to study the biological effect of TTR gene on the growth of hepatoma cells, a recombinant vector containing TTR cDNA was constructed by pCMV, then it was transfected into hepatoma cell lines SMMC-7721 and Q3.It has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50%in strength compared with that of the control. This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid. Hepatoma cells of cell lines PLC/PRF/5, SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization. The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721cells transfected with pCMV alone. However, a remarkable TTR mRNA expression was observed in hepatoma SMMC-7721 cells transfected with pCMV-TTR. It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer. | LIU CHAOTING JIN YAO DAIZONG LI HUIQIU JIANG JIANREN GU.(National Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute, Shanghai 200032, China.) | 1994 | Cell Research1994，4，1： | 0 |
| 14 | Differential expression of a cDNA clone in human liverversus hepatic cancer-highly homologous to aryl-dialkyl-phosphatase显示文摘We applied the technique of mRNA differential display to normal liver tissue and hepatoma cell line Hep3B. One of the isolated cDNA clones was expressed in human normal liver tissue but not in the human hepatocarcinoma cell line. Northern Blot analysis confirmed that high level of mRNA was expressed in human normal liver tissue but the level was decreased in non-cancerous liver tissue from hepatoma patients. Low level or no expression was observed in human hepatoma tissue. One of these transcripts was about 1.8 kb in length. Southern Blot analysis showed that it was a single copy gene. We obtained a full length cDNA clone of 2,395 bp by screening human liver 5’-stretch plus cDNA library. Nucleotide sequence indicated that this clone was highly homologous to aryldialkyl-phosphatase and possessed two polymorphic sites. Aryl-dialkyl--phosphatase which has a prominent role in the metabolism of several toxic, synthetic compounds, may be potentially related to human hepatocarcinoma susceptibility. The biological significance of its differential expression in normal versus malignant tissue is discussed. | WANG KAN KAN DA FANG WAN XIAO KUN QIU PEI XIN LU JIAN REN GU(National La6oratory for Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai 200032)(Qidong Cancer Institute, Jiangsu 226200) | 1997 | Cell Research1997，7，1： | 0 |
| 15 | Cloning, expression and characterization of human tissue-specific DNA polymerase λ2显示文摘DNA polymerase (POL) λ plays an important role during DNA repair and DNA nonhomologous recom-bination processes. A novel POL λ variant was cloned from a human liver cDNA library and named POL λ2 (GenBank Accession No. AY302442). POL λ2 has 2206 base pairs in length with an open reading frame of 1452 base pairs encoding a 482-amino-acids protein. Bioinformatics analysis reveals that POL λ2 spans 7.9 kb on human chromosome 10q24 and is composed of 8 exons and 7 introns. It has the specific domain of DNA polymerase X family-POL Xc at the C-terminus and BRCT domain at the N-terminus. POL λ2 was localized predominantly in nucleus in transfected L0-2 cells. It was expressed abundantly in liver and testis, weakly in ovary, and undetectably in other tested human tissues. In comparison with the expression ratio between POL λ and POL λ2 in normal liver tissues and hepato-cellular carcinoma (HCC) adjacent tissues, the ratio was aberrant in 80% of those 15 HCC specimens examined due to the up-regulated expression of POL λ. This abnormality might be involved in hepato-carcinogenesis. The recombinant POL λ2 with His-tag was expressed as a soluble active protein in E. coli BL21 (DE3)CONDON Plus and purified by Ni-NTA resin and then desalted by Superdex-75 chro-matography in an FPLC system. The analysis using isotope α-32P-dCTP incorporation in vitro showed that the purified recombinant POL λ2 exhibited DNA polymerase activity. | GU Fu1, YOU Chun1, LIU JianPing1, CHEN Ao1,,YU Yao1, WANG Xiang1, WAN DaFang2, GU JianRen2, YUAN HanYing1, LI YuYang1 & Lü Hong1 1 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China 2 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China Present address: Food Inspection and Supervision Institute of Shanxi Province, No. 222 Changzhi Road, Taiyuan 030012, China | 2007 | Science China(Life Sciences)2007，50，4： | 0 |
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