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| 1 | Pharmacokinetics and disposition of anlotinib, an oral tyrosine kinase inhibitor, in experimental animal species显示文摘 | Chen-chun ZHONG Feng CHEN Jun-ling YANG Wei-wei JIA Li LI Chen CHENG Fei-fei DU Su-ping ZHANG Chengying XIE Na-ting ZHANG Olajide E OLALEYE Feng-qing WANG Fang XU Li-guang LOU Dong-ying CHEN Wei NIU Chuan LI | 2018 | Acta Pharmacologica Sinica2018，39，6： | 24 |
| 2 | 绵羊肌细胞生成素基因外显子1的PCR-SSCP分析显示文摘采用PCR-SSCP技术分析了肌细胞生成素(myogenin,MYOG)基因外显子1在小尾寒羊、湖羊、多赛特羊和萨福克羊4个绵羊品种中的多态性。结果表明在引物1中不存在多态性,在引物2中存在3种基因型(AA型、AB型和BB型)。对引物2扩增片段,4个绵羊品种都检测到AA基因型和AB基因型,BB基因型只在小尾寒羊、湖羊和萨福克羊中检测到,而在多赛特羊中没有发现;在4个绵羊品种中,AA基因型频率最高,A等位基因频率均明显高于B等位基因频率。对引物2的多态片段测序分析表明:位于MYOG基因cDNA第183处(GenBank登录号U14331)发生了单碱基突变(C→T),并导致了氨基酸由丙氨酸改变为缬氨酸。 | 储明星 何远清 王金玉 Olowofeso Olajide 叶素成 方丽 | 2005 | 农业生物技术学报2005，13，1： | 23 |
| 3 | Pharmacokinetics and disposition of monoterpene glycosides derived from Paeonia lactiflora roots (Chishao) after intravenous dosing of antiseptic XueBiJing injection in human subjects and rats显示文摘瞄准:从 Paeonia lactiflora 根(Chishao ) 导出的 Monoterpene glycosides 被相信为防腐草药的注射 XueBiJing pharmacologically 重要。这研究被设计描绘 monoterpene glycosides.Methods 的 pharmacokinetics 和布置:到 Chishao monoterpene glycosides 的全身的暴露在收到静脉内的注入和 XueBiJing 注射的多重注入的人的题目被估计,由对主要传播混合物的 pharmacokinetics 的评价列在后面。支持的老鼠研究也被执行。膜渗透和血浆蛋白质绑定在 vitro.Results 被估计:18 monoterpene glycosides 的一个总数在 XueBiJing 注射被检测(内容层次, 0.001-2.47 mmol/L ) ,并且 paeoniflorin 说明了检测的 monoterpene glycosides 的 85.5% 全部的剂量。在人的题目,未改变的 paeoniflorin 与 1.2-1.3 h 的消除一半生活展出了全身的暴露的可观的层次;没有重要代谢物被检测。Oxypaeoniflorin 和 albiflorin 展出了低暴露层次,并且留下的次要的 monoterpene glycosides 可以忽略或未被发现。Glomerular-filtration-based 肾的排泄是 paeoniflorin 的主要消除小径,它血浆蛋白质糟糕一定。在老鼠,当剂量被增加, paeoniflorin 的全身的暴露水平按比例增加了。老鼠肺,心,和 paeoniflorin 的肝暴露层次比血浆水平低,与肾水平的例外,它是比血浆水平大的 4.3 褶层;大脑穿入被差的膜 permeability.Conclusion 限制:由于它的重要全身的暴露和适当 pharmacokinetic 侧面,以及以前报导了防腐性质, paeoniflorin 是治疗学的重要性的有希望的 XueBiJing 成分。 | Chen CHENG Jia-zhen LIN Li LI Jun-ling YANG Wei-wei JIA Yu-hong HUANG Fei-fei DU Feng-qing WANG Mei-juan LI Yan-fen LI Fang XU Na-ting ZHANG Olajide E. OLALEYE Yan SUN Jian LI Chang-hai SUN Gui-ping ZHANG Chuan LI | 2016 | Acta Pharmacologica Sinica2016，37，4： | 15 |
| 4 | Intravenous formulation of Panax notoginseng root extract:human pharmacokinetics of ginsenosides and potential for perpetrating drug interactions显示文摘XueShuanTong,a lyophilized extract of Panax notoginseng roots(Sanqi)for intravenous administration,is extensively used as add-on therapy in the treatment of ischemic heart and cerebrovascular diseases and comprises therapeutically active ginsenosides.Potential for XueShuanTong-drug interactions was determined;the investigation focused on cytochrome P450(CYP)3A induction and organic anion-transporting polypeptide(OATP)1 B inhibition.Ginsenosides considerably bioavailable for drug interactions were identifed by dosing XueShuanTong in human subjects and their interaction-related pharmacokinetics were determined.The CYP3A induction potential was determined by repeatedly dosing XueShuanTong for 15 days in human subjects and by treating cryopreserved human hepatocytes with circulating ginsenosides;midazolam served as a probe substrate.Joint inhibition of OATP1B by XueShuanTong ginsenosides was assessed in vitro,and the data were processed using the Chou-Talalay method,Samples were analyzed by liquid chromatography/mass spectrometry.Ginsenosides Rb1,Rd,and Rg1 and notoginsenoside R were the major circulating XueShuanTong compounds;their interaction-related pharmacokinetics comprised compound dose-dependent levels of systemic exposure and,for ginsenosides Rb,and Rd,long terminal hal-lives(32-57 and 58-307 h,respectively)and low unbound fractions in plasma(0.8%-2.9%and 0.4%-3.0%,respectively).Dosing XueShuanTong did not induce CYP3A.Based on the pharmacokinetics and inhibitory potency of the ginsenosides,XueShuanTong was predicted to have high potential for OATP1B3-mediated drug interactions(attributed chiefly to ginsenoside Rb,)suggesting the need for further model-based determination of the interaction potential for XueShuanTong and,if necessary,a clinical drug interaction study.Increased awareness of ginsenosides'pharmacokinetics and XueShuanTong-drug interaction potential will help ensure the safe use of XueShuanTong and coadministered synthetic drugs. | Salisa Pintusophon Wei Niu Xiao-na Duan Olajide E Olaleye Yu-hong Huang Feng-qing Wang Yan-fen Li Jun-ling Yang Chuan Li | 2019 | Acta Pharmacologica Sinica2019，40，10： | 13 |
| 5 | 红花的化学成分及DPPH自由基清除活性研究显示文摘从红花中分离得到14个化合物,通过波谱数据和理化性质分别鉴定为:异光黄素(1)、(2S)-4',5,6,7-四羟基二氢黄酮-6-O-β-D-葡萄糖苷(2)、新红花苷(3)、山柰酚(4)、山柰酚-3-O-β-D-葡萄糖苷(5)、山柰酚-3-O-β-芸香糖苷(6)、6-羟基山柰酚(7)、6-羟基山柰酚-3-O-β-D-葡萄糖苷(8)、槲皮素(9)、对羟基苯甲酸(10)、对羟基桂皮酸(11)、尿嘧啶(12)、腺嘌呤(13)、β-谷甾醇(14),其中化合物1首次从红花属植物中分离得到。采用TLC-DPPH生物自显影法筛选单体化合物及红花醇提物各萃取部位的自由基清除活性,结果表明乙酸乙酯萃取物及化合物4、9、11具有明显的DPPH自由基清除活性。 | Olaleye Olajide 李珊珊 刘海涛 柴欣 王跃飞 高秀梅 | 2014 | 天然产物研究与开发2014，26，1： | 10 |
| 6 | High degree of pharmacokinetic compatibility exists between the five-herb medicine XueBiJing and antibiotics comedicated in sepsis care显示文摘Managing the dysregulated host response to infection remains a major challenge in sepsis care. Chinese treatment guideline recommends adding Xue Bi Jing, a five-herb medicine, to antibioticbased sepsis care. Although adding Xue Bi Jing further reduced 28-day mortality via modulating the host response, pharmacokinetic herbedrug interaction is a widely recognized issue that needs to be studied.Building on our earlier systematic chemical and human pharmacokinetic investigations of Xue Bi Jing, we evaluated the degree of pharmacokinetic compatibility for Xue Bi Jing/antibiotic combination based on mechanistic evidence of interaction risk. Considering both Xue Bi Jing-antibiotic and antibiotic-Xue Bi Jing interaction potential, we integrated informatics-based approach with experimental approach and developed a compound pair-based method for data processing. To reflect clinical reality, we selected for study Xue Bi Jing compounds bioavailable for drug interactions and 45 antibiotics commonly used in sepsis care in China. Based on the data of interacting with drug metabolizing enzymes and transporters, no Xue Bi Jing compound could pair, as perpetrator, with the antibiotics. Although some antibiotics could,due to their inhibition of uridine 50-diphosphoglucuronosyltransferase 2 B15, organic anion transporters1/2 and/or organic anion-transporting polypeptide 1 B3, pair with senkyunolide I, tanshinol and salvianolic acid B, the potential interactions(resulting in increased exposure) are likely desirable due to these Xue Bi Jing compounds’ low baseline exposure levels. Inhibition of aldehyde dehydrogenase by 7 antibiotics probably results in undesirable reduction of exposure to protocatechuic acid from Xue Bi Jing.Collectively, Xue Bi Jing/antibiotic combination exhibited a high degree of pharmacokinetic compatibility at clinically relevant doses. The methodology developed can be applied to investigate other drug combinations. | Jian Li Olajide E.Olaleye Xuan Yu Weiwei Jia Junling Yang Chuang Lu Songqiao Liu Jingjing Yu Xiaona Duan Yaya Wanga Kai Dong Rongrong He Chen Cheng Chuan Li | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 10 |
| 7 | 新扬州鸡IGF-1基因多态性与早期生长速度关系的研究显示文摘以150只非同胞新扬州鸡为材料,采用PCR-RFLP法检测了该基因5'调控区DNA序列多态性,并运用线性模型统计方法分析了多态性与初生重和12周龄体重的关系。结果显示:新扬州鸡IGF-1基因5'调控区自然存在两种不同DNA序列,经PstI酶切后出现3种基因型('-/-'、'-/+'、'+/+'),基因型分布符合哈代-温伯格定律。各基因型个体的初生重、12周龄体重的最小二乘均数存在'-/-'>'+/-'>'+/+'的趋势,且'-/-'型个体的12周龄体重显著高于'+/+'型个体(P<0.05)。 | 王志跃 范刚 杨海明 汪张贵 盛东风 刘桂琼 Olowofesso Olajide | 2004 | 中国家禽2004，26，24： | 8 |
| 8 | Human pharmacokinetics of ginkgo terpene lactones and impact of carboxylation in blood on their platelet-activating factor antagonistic activity显示文摘Terpene lactones are a class of bioactive constituents of standardized preparations of Ginkgo biloba leaf extract, extensively used as add-on therapies in patients with ischemic cardiovascular and cerebrovascular diseases. This investigation evaluated human pharmacokinetics of ginkgo terpene lactones and impact of their carboxylation in blood. Human subjects received oral YinXing- TongZhi tablet or intravenous ShuXueNing, two standardized ginkgo preparations. Their plasma protein-binding and plateletactivating factor antagonistic activity were assessed in vitro. Their carboxylation was assessed in phosphate-buffered saline (pH 7.4) and in human plasma. After dosing YinXing-TongZhi tablet, ginkgolides A and B and bilobalide exhibited significantly higher systemic exposure levels than ginkgolides C and J; after dosing ShuXueNing, ginkgolides A, B, C, and J exhibited high exposure levels. The compounds’ unbound fractions in plasma were 45–92%. Apparent oral bioavailability of ginkgolides A and B was mostly >100%, while that of ginkgolides C and J was 6–15%. Bilobalide’s bioavailability was probably high but lower than that of ginkgolides A/B. Terminal half-lives of ginkgolides A, B, and C (4–7 h) after dosing ShuXueNing were shorter than their respective values (6–13 h) after dosing YinXing-TongZhi tablet. Half-life of bilobalide after dosing the tablet was around 5 h. Terpene lactones were roughly evenly distributed in various body fluids and tissues; glomerular-filtration-based renal excretion was the predominant elimination route for the ginkgolides and a major route for bilobalide. Terpene lactones circulated as trilactones and monocarboxylates. Carboxylation reduced platelet-activating factor antagonistic activity of ginkgolides A, B, and C. Ginkgolide J, bilobalide, and ginkgo flavonoids exhibited no such bioactivity. Collectively, differences in terpene lactones’ exposure between the two preparations and influence of their carboxylation in blood should be considered in investigating the relative contributions of terpene lactones to ginkgo preparations’ therapeutic effects. The results here will inform rational clinical use of ginkgo preparations. | Xin-wei Liu Jun-ling Yang Wei Niu Wei-wei Jia Olajide E.Olaleye Qi Wen Xiao-na Duan Yu-hong Huang Feng-qing Wang Fei-fei Du Chen-chun Zhong Yan-fen Li Fang Xu Qi Gao Li Li Chuan Li | 2018 | Acta Pharmacologica Sinica2018，39，12： | 8 |
| 9 | 血必净注射液联合抗生素治疗脓毒症:两类药物间高水平药代和谐显示文摘联合用药既需要药物间药效协同互补,也需要药物间能够“药代和谐”(pharmacokinetic compatibility,PKC;不发生会影响药物有效性或安全性的药代性质药物相互作用)。当今世界天然产物制品与化药一同使用既大量存在,又充满争议。一方面,服用葡萄柚汁、圣约翰草制剂等能严重干扰同时进行的化药治疗,这使人们对天然产物制品与化药合用充满戒心;另一方面,越来越多严格的临床和基础研究证明,中药联合化药能更好地应对多因素疾病。脓毒症是一种由感染引起机体反应失调所导致危及生命的器官功能障碍,死亡率高、预后不良。 | 李坚 Olajide EOlaleye 余玄 贾伟伟 杨军令 吕闯 刘松桥 于晶晶 段小娜 王亚亚 董凯 贺容容 程晨 李川 | 2020 | 中国临床药理学与治疗学2020，25，4： | 7 |
| 10 | LC/MS/MS determination and pharmacokinetic studies of six compounds in rat plasma following oral administration of the single and combined extracts of Eucommia ulmoides and Dipsacus asperoides显示文摘AIM: To establish and apply a new LC/MS/MS method for the simultaneous, quantitative determination of six ingredients, aucubin(AU), geniposide(GP), geniposidic acid(GPA), pinoresinol diglucoside(PDG), secologanin(SLG), and loganin(LG) in single and combined extracts of Eucommia ulmoides and Dipsacus asperoides. METHOD: Using the LC/MS/MS-ESI--MRM mode to detect the six compounds, chromatographic separation was achieved on an Agilent Eclipse plus C18 column, and the mobile phase consisted of solvent A(CH3CN) and solvent B(H2O containing 0.01% CH3 COOH V/V). RESULTS: This method was successfully applied to quantify the six compounds in rat plasma after oral administration, and showed good precision, accuracy, reproducibility, and linear regression(r2 >0.99). CONCLUSION: The results showed that following the use of the two medicinal plants, for AU and GP, the values of tmax markedly increased, and the values of cmax markedly decreased. It was found that the compatibility of the medicinal plants might affect their pharmacokinetic properties of their constituents. | HUANG Yu-Xing LIU Er-Wei WANG Lei HUO Yan WANG Qiang OLALEYE Olajide WANG Tao GAO Xiu-Mei | 2014 | Chinese Journal of Natural Medicines2014，12，6： | 7 |
| 11 | Multiple circulating saponins from intravenous ShenMai inhibit OATPIBs in vitro: potential joint precipitants of drug interactions显示文摘ShenMai, an intravenous Injection prepared from steamed Hanax ginseng roots (Hongshen) and Ophiopogon japonicus roots (Maidong), is used as an add-on therapy for coronary artery disease and cancer;saponins are its bioactive constituents. Since many saponins inhibit human organic anion-transporting polypeptides (OATP11B, this investigation determined the inhibition potencies of circulating ShenMai saponins on the transporters and the joint potential of these compounds for ShenMai drug interaction. Circulating saponins and their pharmacokinetics were charaaerized in rats receiving a 30-min infusion of ShenMai at 1OmL/kg. Inhibition of human OATP1B1/1B3 and rat Oatplb2 by the individual saponins was investigated in vitro;the compounds, joint inhibition was also assessed in vitro and the data was processed using the Chou-Talalay method. Plasma protein binding was assessed by equilibrium dialysis. Altogether, 49 saponins in ShenMai were characterized and graded into: 10-1OOμmol/day (compound doses from ShenMai;7 compounds), 1-10 μmol/day (17 compounds), and <1μmol/day (25 compounds, including Maidong ophiopogonins). After dosing, circulating saponins were protopanaxadiol-type ginbenosides Rbi, Rb2r Rc, Rd, Rg2, and Ra3, protopanaxatriol-type ginsenosides Rg1, Re, Rg2, and Rf, and ginsenoside Ro. The protopanaxadiol-type ginsenosides exhibited maximum plasma concentrations of 2.1-46.6 μmol/L, plasma unbound fractions of 0.4-1.0% and terminal half-lives of 15.6-28.5 h (ginsenoside Rg3f 1.9 h), while the other ginsenosides exhibited 0.1-77 μmol/L, 20.8-99.2%, and 0.2-0.5 h, respectively. The protopanaxadiol-type ginsenosides, ginsenosides without any sugar attachment at C-20 (except ginsenoside Rf), and ginsenoside Ro inhibited OATP1B3 more potently (IC50, 0.2-3.5 (μmol/L) than the other ginsenosides (≥22.6 μmol/L). Inhibition of OATP1B1 by ginsenosides was less potent than OATP1B3 inhibition. Ginsenosides Rb1;Rb2, Rc, Rd, Ro, Ra1, Re, and Rg2 likely contribute the major part of OATP1B3-mediated ShenMai-drug interaction potential, in an additive and time-related manner. | Olajide E. Olaleye Wei Niu Fei-fei Du Feng-qing Wang Fang Xu Salisa Pintusophon Jun-lan Lu Jun-ling Yang Chuan Li | 2019 | Acta Pharmacologica Sinica2019，40，6： | 7 |
| 12 | 中药多成分药代动力学:发现与中药安全性和有效性关联的物质并揭示其药代特征显示文摘中医药对中华民族健康和国家稳定发挥了重要作用,揭示决定中药有效性和安全性的物质是推进中药现代化的一项重要工作。对于化学组成复杂的中药,可通过开展多成分药代研究,根据给药后中药成分能否以某种形式被机体利用产生显著的体内暴露(以成分原形和/或代谢物形式),选拔出用于考察药效活性的中药物质,研究物质产生疗效的体内过程和药代特征,由此为揭示决定中药药效作用的物质创造条件。此外,这类多成分药代研究还可用于揭示与中药不良反应或联合用药风险关联的中药物质。经过十多年的努力,中药多成分药代研究在理论、方法、技术、应用上已取得突破,成为药代动力学的一个新分支。本文系统阐述了中药多成分药代动力学的研究方法、技术要求和分析技术,并用一类活性中药成分(三七的皂苷类成分)的研究和围绕一种已上市中药制剂(连花清瘟胶囊)的研究介绍了两类多成分药代研究实例,最后讨论了中药多成分药代研究的进一步发展。 | 李川 程晨 贾伟伟 杨军令 余玄 Olajide E.OLALEYE | 2021 | 药学学报2021，56，9： | 7 |
| 13 | Pharmacokinetics-based identification of pseudoaldosterogenic compounds originating from Glycyrrhiza uralensis roots(Gancao)after dosing LianhuaQingwen capsule显示文摘LianhuaQingwen capsule,prepared from an herbal combination,is officially recommended as treatment for COVID-19 in China.Of the serial pharmacokinetic investigations we designed to facilitate identifying LianhuaQingwen compounds that are likely to be therapeutically important,the current investigation focused on the component Glycyrrhiza uralensis roots(Gancao).Besides its function in COVID-19 treatment,Gancao is able to induce pseudoaldosteronism by inhibiting renal 11β-HSD2.Systemic and colon-luminal exposure to Gancao compounds were characterized in volunteers receiving LianhuaQingwen and by in vitro metabolism studies.Access of Gancao compounds to 11β-HSD2 was characterized using human/rat,in vitro transport,and plasma protein binding studies,while 11β-HSD2 inhibition was assessed using human kidney microsomes.LianhuaQingwen contained a total of 41 Gancao constituents(0.01-8.56μmol/day).Although glycyrrhizin(1),licorice saponin G2(2),and liquiritin/liquiritin apioside(21/22)were the major Gancao constituents in LianhuaQingwen,their poor intestinal absorption and access to colonic microbiota resulted in significant levels of their respective deglycosylated metabolites glycyrrhetic acid(8),24-hydroxyglycyrrhetic acid(M2_(D);a new Gancao metabolite),and liquiritigenin(27)in human plasma and feces after dosing.These circulating metabolites were glucuronized/sulfated in the liver and then excreted into bile.Hepatic oxidation of 8 also yielded M2_(D).Circulating 8 and M2D,having good membrane permeability,could access(via passive tubular reabsorption)and inhibit renal 11β-HSD2.Collectively,1 and 2 were metabolically activated to the pseudoaldosterogenic compounds 8 and M2_(D).This investigation,together with such investigations of other components,has implications for precisely defining therapeutic benefit of LianhuaQingwen and conditions for its safe use. | Xiao-fang Lan Olajide EOlaleye Jun-lan Lu Wei Yang Fei-fei Du Jun-ling Yang Chen Cheng Yan-hong Shi Feng-qing Wang Xue-shan Zeng Nan-nan Tian Pei-wei Liao Xuan Yu Fang Xu Ying-fei Li Hong-tao Wang Nai-xia Zhang Wei-wei Jia Chuan Li | 2021 | Acta Pharmacologica Sinica2021，42，12： | 6 |
| 14 | 华东4个鹌鹑群体微卫星标记的遗传多样性检测(英文)显示文摘在DNA水平上检测了华东地区4个亲源关系不同的鹌鹑群体的3个微卫星座位遗传变异,每一位点均检测到4~5个等位基因,各位点的基因多态比例接近100%。为检测这一地区鹌鹑遗传多态性水平,估计了每个位点的基因杂合度和各群体的基因平均杂合度。结果表明:基因平均杂合度为(0.462 7±0.03)^(0.634 5±0.05),4个群体的平均值按由小到大排列分别为0.462 7、0.514 6、0.554 9和0.634 5,平均有效等位基因数为(1.868 8±0.12)^(2.798 1±0.43),平均多态信息含量为0.376 7~0.571 3,累积辨别力达到95.76%;聚类分析表明,华东地区鹌鹑群体间存在高水平的遗传变异,微卫星标记检测鹌鹑群体间的遗传多样性非常合适。 | Olowofeso Olajide 戴国俊 王金玉 谢恺舟 李宁川 何远清 | 2006 | 扬州大学学报(农业与生命科学版)2006，27，1： | 5 |
| 15 | 狼尾草根系对阿特拉津长期胁迫的氧化应激响应显示文摘通过盆栽实验研究了抗性植物狼尾草根部丙二醛(MDA)、脯氨酸(Pro)、抗坏血酸(As A)含量及超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)等氧化应激生理指标对不同浓度阿特拉津长期(48 d)胁迫的响应规律。结果表明:当阿特拉津胁迫浓度分别高于20 mg·kg^(-1)和50 mg·kg^(-1)时,狼尾草根系的MDA与Pro含量较对照组显著升高(P<0.05);随着阿特拉津胁迫浓度的增加,狼尾草根部SOD和GR活性呈先升高后降低的趋势,其中当阿特拉津胁迫浓度为20 mg·kg^(-1)时,SOD和GR活性达到最大值;供试植物根系中As A含量与阿特拉津胁迫浓度呈正相关。综上,中低浓度(≤20 mg·kg^(-1))阿特拉津处理没有对狼尾草的根系产生明显的氧化胁迫效应,狼尾草根系的上述抗氧化应激生理指标对于发挥阿特拉津抗性起着重要的作用。 | 马兵兵 姜昭 Kehinde Olajide Erinle 曹博 李金梅 陈玉坤 张颖 | 2016 | 生态毒理学报2016，11，6： | 5 |
| 16 | Short Term Influence of Organic and Inorganic Fertilizer on Soil Microbial Biomass and DNA in Summer and Spring显示文摘The present study was conducted to see the short term impact of organic and inorganic fertilizers on soil microbial biomass both in spring and summer. Also aimed to observe the correlation between soil microbial biomass and soil DNA. The study concluded that type of fertilizer might alter the soil microbial biomass and DNA contents. In soil treated with organic fertilizers resulted in higher concentrations of microbial biomass and DNA contents in summer as compared to spring dute to increase in temperature. Correspondingly, in case of inorganic fertilizer, concentrations of soil microbial biomass and DNA detected higher in summer instead of spring. The statistical correlation between soil microbial biomass, DNA and ODR in spring and summer along with organic and inorganic fertilizers were calculated highly significant(p>0.01). This study demonstrated the impact of fertilizers and seasonal variations on soil microbial biomass and also revealed significant correlation between soil microbial biomass and soil DNA. | Khalil ur Rehman Zhang Ying Shahla Andleeb Zhao Jiang Erinle Kehinda Olajide | 2016 | Journal of Northeast Agricultural University(English Edition)2016，23，1： | 4 |
| 17 | OPAY02-C型标记与新扬州鸡早期增重关系的研究显示文摘随机选择新扬州鸡5个父系半同胞家系后代78只,用于分析RAPD标记与新扬州鸡早期增重的关系。RAPD标记是在21个随机引物中,经反复试验选出的稳定的,具有多态性的3个引物OPAY02,OPAY13,OPAY17扩增产物,统计分析表明:OPAY02引物扩增的1660bp和2326bp两条多态性条带的4种组合中,OPAY02-C型标记具有显著的增效作用(P<0.015),可作为标记进行辅助选择。 | 戴国俊 王金玉 王志跃 盛浩伟 Olajide 谢恺舟 | 2004 | 中国家禽2004，26，z1： | 3 |
| 18 | 白藜芦醇潜在的有益健康作用:药代动力学带来的困惑显示文摘白藜芦醇(Resveratrol,3,5,4'-三羟基-反式-二苯乙烯)最早在植物白藜芦(Veratrum grandiflorum O.Loes)的根中被发现。白藜芦醇引起关注最初与“法国悖论”有关,该化合物不仅存在于红葡萄酒中,而且还展现出与红葡萄酒保护心血管作用相关的生物活性。除保护心血管方面的作用外,白藜芦醇还在抗代谢性疾病、抗肿瘤和预防神经退行性疾病等方面表现出有益的作用。为了将这些潜在的有益健康作用向临床转化,人们对白藜芦醇开展了药代研究。本文作者从进入体循环的系统暴露和体内过程两个方面,总结了目前已知的口服后白藜芦醇的人体药代特征及围绕提高其系统暴露水平所做的多种尝试。然而,既有的药代动力学结果给白藜芦醇潜在作用的临床转化带来了困惑,包括:白藜芦醇展现有益健康的体外生物活性的物质形式(原形化合物)与口服后被机体利用的体内暴露形式(以代谢物为主)显著不同、药代研究测出的口服后白藜芦醇体内暴露水平明显低于其展现活性所需的浓度、根据白藜芦醇产生体内效应时所用的剂量推导出的体内暴露水平常明显低于研究其作用机制所用的浓度。为了更好地与白藜芦醇有益健康作用的研究相结合、促进其临床转化,作者建议从三方面进一步开展白藜芦醇的药代研究:(1)全面研究白藜芦醇代谢物的系统暴露、体内靶标到达及在靶细胞中的代谢,(2)研究口服后白藜芦醇的肠腔暴露,(3)开展红葡萄酒多成分药代研究。 | 王亚亚 褚子璇 杨军令 Olajide E.Olaleye 贺容容 李木子 程晨 李川 | 2021 | 中国临床药理学与治疗学2021，26，8： | 3 |
| 19 | 微卫星PCR扩增反应技术条件优化探讨显示文摘微卫星PCR扩增反应受诸多因素的影响,不同的Mg2+浓度、退火温度、引物浓度、dNTP浓度、模板量、不同厂家的TaqDNA聚合酶、不同的PCR仪等都会对扩增反应结果有不同程度的影响。在进行PCR反应前,须对这些条件反复摸索优化,以获得最佳反应体系。 | 盛浩伟 王金玉 戴国俊 Olowofeso Olajide | 2004 | 四川畜牧兽医2004，31，4： | 2 |
| 20 | Influence of Soil pH and Temperature on Atrazine Bioremediation显示文摘Present study was conducted to clarify soil pH and temperature influence on different atrazine bioremediation techniques. For this purpose, sodium citrate, Arthrobactor sp. strain DNS10, sawdust and animal manure were selected to clarify their atrazine remediation efficiency under pH 5, 7 and 9 and temperatures 20, 30 and 40℃, respectively. Results showed that atrazine remediation was generally optimized at pH 7 and 30℃ for all the treatments except sodium citrate as soil treated with sawdust was not temperature dependant, but at pH 5 remediation process was determined slower. Atrazine remediation in soil with no additional amendment was only 34%, while in soil treated with sawdust, DNS10, sodium citrate and animal manure were 75.17%, 89%, 74.17% and 76.83% at optimized pH and temperature. Overall atazine removal rate was significantly(≥0.01) higher with increasing in temperature at all the selected pH. | Shahla Andleeb Zhao Jiang Khalil ur Rehman Erinle Kehinda Olajide Zhang Ying | 2016 | Journal of Northeast Agricultural University(English Edition)2016，23，2： | 2 |
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