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| 1 | Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum显示文摘Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions. | YANG Xiu XU Yanchao YANG Fangfang MAGWANGA Richard Odongo CAI Xiaoyan WANG Xingxing WANG Yuhong HOU Yuqing WANG Kunbo LIU Fang ZHOU Zhongli | 2019 | Journal of Cotton Research2019，2，2： | 7 |
| 2 | In vitro Ruminal Gas Production Kinetics of Four Fodder Trees Ensiled With or Without Molasses and Urea显示文摘This study investigated if the addition of urea (U), molasses (M) or their 1:1 (v/v) mixture during ensiling increases the nutritional value of forage from four fodder trees (Prunus persica, Leucaena esculenta, Acacia farnesiana, and Prunus domestica). Forage samples of fodder trees were collected in triplicate (three individual samples of each species) and subjected to an in vitro gas production (GP) procedure. Fermentation at 24 h (GP 24), short-chain volatile fatty acids (SCFA), and microbial crude protein production (MCP), in vitro organic matter digestibility (OMD), metabolizable energy (ME) and dry matter degradability (DMD) were estimated. Forage samples were incubated for 72 h in an incubator at 39oC and the volume of GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, and 72 h of incubation using the reading pressure technique. The rumen fermentation profiles were highest for P. persica, which showed the highest (P<0.0001) DMD, ME, OMD, SCFA, GP 24 and MCP. On the other hand L. esculenta had the lowest (P<0.0001) DMD, SCFA, MCP; P. domestica had the lowest (P<0.0001) OMD. The addition of M to silage increased (P<0.0001) ME and OMD, as well as GP. However, the addition of U and the mixture of U and M reduced (P<0.0001) DMD, ME, OMD, SCFA, GY 24 and MCP. These results show that P. persica has the highest nutritive value and L. esculenta the lowest for ruminants. Additionally, the addition of M to forage from fodder trees increases rumen GP and fermentation, which may improve nutrient utilization in ruminants. | Abdelfattah Z M Salem ZHOU Chuan-she TAN Zhi-liang Miguel Mellado Moises Cipriano Salazar Mona M M Y Elghandopur Nicholas E Odongo | 2013 | Journal of Integrative Agriculture2013，12，7： | 3 |
| 3 | 棉属D基因组3个野生棉种Bet v1基因鉴定及功能分析显示文摘【目的】对棉花D基因组中Bet v 1基因家族进行分析,比较其在不同抗性棉种间的表达模式差异,为深入研究Bet v 1基因在棉花抗黄萎病中的作用提供理论依据。【方法】通过生物信息学方法对D基因组中Bet v 1基因进行鉴定。通过雷蒙德氏棉(Gossypium raimondii,D5)、三裂棉(G.trilobum,D8)和瑟伯氏棉(G.thurberi,D1)转录组和实时荧光定量聚合酶链式反应(qRT-PCR)分析Bet v 1基因在黄萎病菌处理下的表达模式。利用病毒诱导的基因沉默技术(VIGS)对Bet v 1基因进行功能鉴定。【结果】[棉花D基因组中包含59个成员,其中57个基因带有内含子,分布于8条染色体上,多数为亲水性蛋白并定位于细胞质。]黄萎病菌胁迫条件下3个野生棉种的Bet v 1基因表达量与其抗病水平一致。将不同表达水平的基因分为3组,其中第3组基因响应黄萎病菌侵染并在抗病棉种瑟伯氏棉中高表达,表明该组基因可能与黄萎病胁迫应答反应有关。从中筛选出高水平表达的Bet v 1基因,在陆地棉中沉默相应的同源基因,棉株感病加重,揭示该基因在棉花抵御黄萎病菌侵染过程中起正调控作用。【结论】响应黄萎病菌胁迫的Bet v 1基因在棉花抗黄萎病复杂的生物过程中至关重要。本研究结果为棉花Bet v 1家族基因的深入研究提供依据,为进一步解析棉花Bet v 1基因的功能奠定基础。 | 董琪 Magwanga Richard Odongo 路普 蔡小彦 周忠丽 王省芬 王星星 许艳超 侯宇清 王艳情 王坤波 刘方 马峙英 | 2019 | 棉花学报2019，31，5： | 3 |
| 4 | Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton显示文摘Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production. | MAGWANGA Richard Odongo LU Pu KIRUNGU Joy Nyangasi CAI Xiaoyan ZHOU Zhongli AGONG Stephen Gaya WANG Kunbo LIU Fang | 2020 | Journal of Cotton Research2020，3，1： | 2 |
| 5 | Phosphorus utilization and environmental and economic Implications of reducing phosphorus pollution from ontario dairy cows显示文摘 | Kebreab E Odongo N E McBride B W | 2008 | J Dairy Sci2008，91，： | 1 |
| 6 | Pre- diction of methane production from dairy and beef cat- tle 显示文摘 | ELLIS J L KEBREAB E ODONGO N E | 2007 | Journal of Dairy Science2007，90，7： | 1 |
| 7 | Effects of monensin and dietary soybean oil on milk fat percentage and milk fatty acid profile in lactating dairy cows显示文摘 | AlZahal O Odongo N E Mutsvangwa T | 2008 | J Dairy Sci2008，91，： | 1 |
| 8 | Improving rock phosphate availability through feeding, mixing and processing with composting manure 显示文摘 | Odongo N E Ho K H Choi H C | 2007 | Bioresource Technology2007，98，： | 1 |
| 9 | Effects of monensin and dietary soybean oil on milk fat percentage and milk fatty acid profile in lactating dairy cows显示文摘 | Alzahal O Odongo N E Mutsvangwal T | 2008 | J Dairy Sci2008，91，： | 1 |
| 10 | Long term effects of feeding monensin on methane production in lactating dairy cows 显示文摘 | Odongo N E Bagg R Vessie G | 2007 | Journal of Dairy Science2007，90，4： | 1 |
| 11 | Long-term effects of feeding monensin on milk fatty acid composition in lactating dairy cows显示文摘 | ODONGO N E OR-RASHID M M BAGG R | | 0，，11： | 1 |
| 12 | Effects of rumen acid load from feed and forage particle size on ruminal pH and dry matter intake in the lactating dairy cow显示文摘 | Rustomo B AlZahal O Odongo N E | | 0，，： | 1 |
| 13 | Effects of Rumen Acid Load from Feed and Forage Particle Size on Ruminal pH and Dry Matter Intake in the Lactating Dairy Cow显示文摘 | B. Rustomo O. AlZahal N.E. Odongo T.F. Duffield B.W. McBride | 2006 | Journal of Dairy Science2006，，12： | 1 |
| 14 | Management of windbreaks in the Sahel:the strategic implications of tree water use显示文摘 | Smith D M Jarvis P G Odongo J W | 1998 | Agroforestry Systems1998，40，： | 1 |
| 15 | Diallel analysis of grain yield and resistance to seven diseases of 12 African maize inbred lines显示文摘 | Vivek B S Odongo O Njuguna J | 2010 | Euphytica2010，172，： | 1 |
| 16 | Fatty acid composition of ruminal bacteria and protozoa, with emphasis on conjugated linoleic acid, vaccenic acid, and odd-chain and branched chain fatty acids 显示文摘 | Or-Rashid M M Odongo N E McBride B W | 2007 | J Anim Sci2007，85，5： | 1 |
| 17 | Studies on the maturation-accelerating pheromone of the desert locustSchistocerca gregaria (Orthoptera: Acrididae)显示文摘 | Hassane Mahamat Ahmed Hassanali Hezekiel Odongo Baldwyn Torto El-Sayeed El-Bashir | 1993 | Chemoecology (-)1993，，3： | 1 |
| 18 | Long term ef ects of feeding mon-ensin on methane production in lactating dairy cows显示文摘 | Odongo N E Bagg R Vessie G | | 0，，04： | 1 |
| 19 | Phosphorus utilization and environmental and economic implications of reducing phosphorus pollution from ontario dairy cows 显示文摘 | Kebreab E Odongo N E McBride B W | 2005 | J Dairy Sci2005，91，： | 1 |
| 20 | Improving rock phosphate availability through feeding mixing and processing with composting manure显示文摘 | Odongo N E Hyoung-ho K Choi H | 2007 | Bioresource Technology2007，98，15： | 1 |
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