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| 1 | Modulations of genes related to gut integrity,apoptosis,and immunity underlie the beneficial effects of Bacillus amyloliquefaciens CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model显示文摘Background:The ban of in-feed antimicrobial additives has negatively affected the poultry industry by causing necrotic enteritis(NE)to emerge in the flocks.Alternatives such as Bacillus probiotics have shown to be effective on eliminating the negative effects of this disease.Two experiments were conducted to investigate the effect of Bacillus amyloliquefaciens CECT 5940(BA)in broiler chickens under NE challenge and/or fed diets with different protein levels.Methods:In both experiments,480 day-old mix-sexed Ross-308 broilers were arranged in a 2×2 factorial arrangement of treatments.In experiment 1,the factors were NE challenge(yes or no)and probiotic(yes or no).In experiment 2,the factors were dietary crude protein levels(standard or reduced)and probiotic(yes or no)and were used under NE challenge condition.Oral administration of Eimeria oocysts(day 9)followed by inoculation with Clostridium perfringens(day 14 and 15)was used to induce NE challenge.On day 16,two birds from each treatment were gavaged with fluorescein isothiocyanate-dextran(FITC-d)and blood samples were collected for gut integrity evaluation,and jejunal samples were collected for gene expression assay.Results:In experiment 1,BA supplementation decreased caspase-3(CASP3)(P<0.001)and caspase-8(CASP8)(P<0.05)and increased occludin(OCLD)(P<0.05)expression regardless of the challenge.Additionally,BA supplementation downregulated interfron-γ(IFN-γ)expression(P<0.01)and upregulated immunoglobulin-G(IgG)(P<0.01)and immunoglobulin-M(IgM)(P<0.05)only in challenged birds.In experiment 2,the expression of genes encoding mucin-2(MUC2)(P<0.001),tight junction protein-1(TJP1)(P<0.05)and OCLD(P<0.05)were upregulated by the addition of BA in the diet,regardless of the crude protein level.Further,BA supplementation downregulated INF-γ(P<0.01)and upregulated immunoglobulin-A(IgA)(P<0.05),IgM(P<0.05)and IgG(P<0.01)regardless of the crude protein level.Conclusion:These findings suggest that supplementation of BA in broiler diets can improve gut health by modulation of genes related to the mucosal barrier,tight junction,and immunity in broilers challenged by unfavourable conditions such as NE challenge. | Kosar Gharib-Naseri Juliano Cesar de Paula Dorigam Kiran Doranalli Sarbast Kheravii obert A.Swick Mingan Choct Shu-Biao Wu | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 6 |
| 2 | 携带血管内皮生长因子的蛋白涂层支架预防冠状动脉球囊扩张后再狭窄的预防显示文摘目的 :评价蛋白涂层支架携带血管内皮生长因子 (VEGF)预防冠状动脉成形术后再狭窄的效果。方法 :金属支架涂层为胶联明胶制成。应用标准球囊导管技术 ,将包被有VEGF的涂层支架置入小型猪 (n =10 )冠状动脉前降支中段 ,以相同方法置入单纯蛋白涂层支架作为对照组 (n =10 ) ,支架与血管直径之比为 1.1~ 1.3∶1。结果 :在支架置入后 3个月时冠状动脉造影显示 :VEGF组无再狭窄发生 ,而对照组均发生显著的再狭窄。组织病理学形态分析结果显示 :VEGF组新生内膜面积 (1.8± 0 .6 )mm2 ,平均百分狭窄面积 (2 5 .9± 6 .5 ) % ,平均管腔狭窄百分数 (40 .4± 13.7) % ,均较对照组〔分别为 (2 .6± 0 .7)mm2 ,P <0 .0 5 ;(93.1± 11.5 ) % ,P <0 .0 1;(88.2± 14 .4 ) % ,P <0 .0 1〕小 ;内膜面积 /中膜面积比值 ,治疗组较对照组减少了 5 5 .3%。结论 :在小型猪模型使用蛋白涂层包被VEGF支架 ,能预防内膜过度增殖 ,从而预防再狭窄的发生。 | 卢英民 ELABAZM OBERTER 毛焕元 | 2002 | 临床心血管病杂志2002，18，5： | 2 |
| 3 | Temporomandibular joint-preserving preauricular subtemporal-infratemporal fossa approach:surgical technique and clinical application显示文摘 | MARCELO D OBERT C | 2004 | Neurosurgery2004，55，1： | 1 |
| 4 | Fixation of distal radius fractures in adults: a review显示文摘 | OBERT L REY PB UHRING J | 2013 | Orthopaedics & Traumatology Surgery & Research : OTSR2013，99，2： | 1 |
| 5 | Genetic transformation of barley microspores using anther bombardment 显示文摘 | Obert B Middlefell-Williams J Millam S | 2008 | Bioteeh Lett2008，30，： | 1 |
| 6 | Multisection dynamic CT perfusion for acute cerebral iscbemia: the 'toggling -table' technique显示文摘 | Oberts HC Roberts TP Smith WS | 2001 | AJNR Am J Neureradiol2001，22，6： | 1 |
| 7 | Aerodynamic Design of Transport Air- craft显示文摘 | Ed Obert | | Delft University0，，： | 1 |
| 8 | Effect of longterrm intense swimming training on the upper body peak oxgen uptake of prepubertal girls显示文摘 | OBERT P COURTEIX D LECOQ A M | 1996 | Eur J Appl Physiol Occup Physiol1996，73，12： | 1 |
| 9 | Burgel Pneumococcal infections: association with asthma and COPD显示文摘 | Obert J Burgel PR | 2012 | Med Mal Infect2012，42，5： | 1 |
| 10 | D esign and perform ance of a 215 GHz pulsed radar system 显示文摘 | R obert E M cln T osh J am es B M ead | 1988 | IEEE Transactions onMicrowave Theory and T e c h n ic s1988，36，6： | 1 |
| 11 | New drying process for lactic bacteria based on their dehydration behavior in liquid medium显示文摘 | MILLE Y OBERT J P BENEY L | 2004 | Biotechnology and Bioengineering2004，88，1： | 1 |
| 12 | Cluster formation in expanding supersonic jet: effect of pressure, temperature, nozzle size and test gas显示文摘 | Hagena O F Obert O | 1972 | J Chem Phys1972，56，5： | 1 |
| 13 | Adult tibial eminence fracture fixation: arthroscopic procedure using K-wire folded fixation显示文摘 | Nicolas Bonin Laurent Jeunet Laurent Obert David Dejour | 2007 | Knee Surgery Sports Traumatology Arthroscopy2007，，7： | 1 |
| 14 | Long- term endurance training dose not prevent the age- related decrease in left ventricular relaxation properties 显示文摘 | Nottin S Nguyen LD Terbah M Obert P | 2004 | Acta Physiol Scand2004，181，2： | 1 |
| 15 | Adult tibial eminence frac- ture fixation: arthroscopic procedure using K - wire folded fixation 显示文摘 | Boin N Jeunet L Obert L | 2007 | Knee Surg Sports Traumatol Arthrosc2007，15，7： | 1 |
| 16 | Estimation of the von Willebrand factor-cleaving protease in plasma using monoclonal antibodies to VMF显示文摘 | Obert B | 1999 | Thromb Haemost1999，82，11： | 1 |
| 17 | Association of AFLP markers with downy mildew resistance in autotetraploid alfalfa 显示文摘 | Obert D E Skinner D Z Stuteville D L | 2000 | Molecular Breeding2000，6，： | 1 |
| 18 | Specific von Willebrand factor-cleaving protease in thrombotic microangiopathies:a study of 111 cases显示文摘 | Obert B Houllier A | 2001 | Blood2001，98，6： | 1 |
| 19 | Adult tibia1 eminence fracture fixation: arthroscopic procedure using K-wire folded fixation 显示文摘 | Bonin N Jeunet L Obert L | 2007 | Knee Surg Sports Traumatol Arthrosc2007，15，7： | 1 |
| 20 | Association of AFLP markers with downy mildew resistance in autotetraploid alfalfa显示文摘 | D.E. Obert D.Z. Skinner D.L. Stuteville | 2000 | Molecular Breeding2000，，3： | 1 |
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