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您的检索式：作者名="Ningyan Shen"
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| 1 | Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport显示文摘The most obvious symptom of Al toxicity is the inhibition of root growth. However, the mechanism of Al-inhibiting root growth remains to be elucidated. In this study, auxin transport and vesicle movement of an auxin-efflux carrier (PIN2) were investigated in Arabidopsis roots in response to Al stress. Results indicated that Al inhibited the apical transport of auxin in root tips of Arabidopsis significantly. The severe inhibition was localized in the cells of transition zone, where the concentration of auxin was only 34% that of the control. Brefeldin A (BFA), an inhibitor of vesicle transport, induced the dot-like structure of PIN2 vesicle significantly. Al decreased the size of dot-like structure of PIN2 vesicles. Re- sults of real-time RT-PCR and Western-blotting analysis showed that Al increased the transcript level of PIN2 and the accumulation of PIN2 protein in horizontal direction of plasma membrane, but decreased its distribution in endosomes, suggesting that Al inhibited the transport of PIN2 vesicles from plasma membrane to endosomes. Results of cytoskeleton-depolymering drugs indicated that it was via the pathway of disruption of actin microfilaments that Al inhibited the transport of PIN2 vesicles. Exposed to Al stress, the cells of elongation zone had less Al uptake and less transport frequency of vesicles than cells of transition zone. Taken together, our results suggested that Al inhibited root growth mainly by modulating the transport of PIN2 vesicles between plasma membrane and endosomes, thus block- ing auxin transport and root growth. | SHEN Hong HOU NingYan Markus SCHLICHT WAN YingLang Stefano MANCUSO Frantisek BALUSKA | 2008 | Chinese Science Bulletin2008，53，16： | 9 |
| 2 | 个体特征和常见疾病与中老年职工工作能力的关系显示文摘为了揭示中老年职工工作能力的影响因素 ,采用病例对照研究方法和危险度分析技术 ,对个体特征、常见疾病与中老年职工工作能力间的关系作了分析研究。结果发现 ,个体特征和疾病与中老年职工工作能力减退有密切关系。非正常婚姻 (OR=2 .5 9)和吸烟 (OR=1.99)表现为工作能力减退的危险因素 ,受教育程度高 (0 .49)和身体锻炼 (OR=0 .78)为保护因素。患有慢性疾病者工作能力减退的危险度明显增加 ,其中以神经感觉系统疾病的相对危险度最高 ,肌肉骨骼疾病、呼吸系统疾病、精神疾病也具有较高的相对危险度。 | 兰亚佳 王治明 王绵珍 詹承烈 Juhani Ilmarinen Matti Klockars Kaija Tuomi Ningyan Shen | 2000 | 华西医科大学学报2000，31，4： | 7 |
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