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| 1 | Deficient DNA mismatch repair is associated with favorable prognosis in Thai patients with sporadic colorectal cancer显示文摘AIM:To determine the prognostic significance of deficient mismatch repair(d MMR) and BRAF V600 E in Thai sporadic colorectal cancer(CRC) patients.METHODS:We studied a total of 211 out of 405 specimens obtained from newly diagnosed CRC patients between October 1,2006 and December 31,2007 at Siriraj Hospital,Mahidol University.Formalinfixed paraffin-embedded blocks of CRC tissue samples w e re a n a l y ze d fo r d M M R b y d e t e c t i o n o f M M R protein expression loss by immunohistochemistry or microsatellite instability using polymerase chain reaction(PCR)-DHPLC.BRAF V600 E mutational analysis was performed in DNA extracted from the same archival tissues by two-round allele-specific PCR and analyzed by high sensitivity DHPLC.Associations between patient characteristics,MMR and BRAF status with diseasefree survival(DFS) and overall survival(OS) were determined by Kaplan-Meier survival plots and log-rank test together with Cox's proportional hazard regression.RESULTS:d MMR and BRAF V600 E mutations were identified in 31 of 208(14.9%) and 23 of 211(10.9%) tumors,respectively.d MMR was more commonly found in patients with primary colon tumors rather than rectal cancer(20.4% vs 7.6%,P =0.01),but there was no difference in MMR status between the right-sided and left-sided colon tumors(20.8% vs 34.6%,P = 0.24).d MMR was associated with early-stage rather than metastatic disease(17.3% vs 0%,P = 0.015).No clinicopathological features such primary site or tumor differentiation were associated with the BRAF mutation.Six of 31(19.3%) samples with d MMR carried the BRAFmutation,while 17 of 177(9.6%) with proficient MMR(p MMR) harbored the mutation(P = 0.11).Notably,patients with d MMR tumors had significantly superior DFS(HR = 0.30,95%CI:0.15-0.77; P = 0.01) and OS(HR = 0.29,95%CI:0.10-0.84; P = 0.02) compared with patients with p MMR tumors.By contrast,the BRAF V600 E mutation had no prognostic impact on DFS and OS.CONCLUSION:The prevalence of d MMR and BRAF V600 E in Thai sporadic CRC patients was 15% and 11%,respectively.The d MMR phenotype was associated with a favorable outcome. | Krittiya Korphaisarn Ananya Pongpaibul Chanin Limwongse Ekkapong Roothumnong Wipawi Klaisuban Akarin Nimmannit Artit Jinawath Charuwan Akewanlop | 2015 | World Journal of Gastroenterology2015，21，3： | 4 |
| 2 | Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes显示文摘Objective: The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3 T3-L1 preadipocytes/adipocytes as a model.Methods: Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3 T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red 0 staining and visualized under a light microscope.Results: n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 μg/ml. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentrationdependent inhibitory effects on lipid accumulation in 3 T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 μmol/L, respectively. Both kaempferide and 4,2'-dihy droxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.Conclusion: C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3 T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids. | Supakanya Kumkarnjana Rutt Suttisri Ubonthip Nimmannit Thongchai Koobkokkruad Chutichot Pattamadilok Nontima Vardhanabhuti | 2018 | Journal of Integrative Medicine2018，16，6： | 2 |
| 3 | Hydrogen per oxide inhibits non-small cell lung cancer cell anoikis through the inhibition of eaveolin-1 degradation显示文摘 | Rungtabnapa P Nimmannit U Halim H | 2011 | Am J Physiol Cell Physiol2011，300，2： | 1 |
| 4 | Mitochondral superoxide mediates doxorubicin-induccd kera- tinocyte apoptosis through oxidative modification of ERK and Bcl-2 ubiquitination 显示文摘 | LUANPITPONG S CHANVORACHOTE P NIMMANNIT U | 2012 | Biochem Pharmacol2012，83，12： | 1 |
| 5 | Hydrogen peroxide inhibits non-small cell lung cancer cellanoikis through the inhibition of caveolin-1 degradation显示文摘 | RUNGTABNAPA P NIMMANNIT U HALIM H | 2011 | Am J Physiol Cell Physiol2011，300，2： | 1 |
| 6 | Arrhythmogenic marker for the sudden unexplained death syndrome in Thai men 显示文摘 | Nademanee K Veerakul G Nimmannit S | 2009 | Circulation2009，96，8： | 1 |
| 7 | C-reactive protein as a monitor of chemotherapy response in advanced non-small cell lung cancer(CML study)显示文摘 | SRIMUNINNIMIT V ARIYAPANYA S NIMMANNIT A | 2012 | J Med Assoc Thai2012，2，： | 1 |
| 8 | 显示文摘 | Veerakul G Nimmannit S | 1997 | Circulation1997，96，8： | 1 |
| 9 | Nitric oxide regulates cell sensitivity to cisplatin-induced apoptosis through Snitrosylation and inhibition of Bcl-2 ubiquitination显示文摘 | Chanvorachote P Nimmannit U Stehlik C | 2006 | Cancer Res2006，66，12： | 1 |
| 10 | Preparation of turmeric oil-loaded chitosanalginate biopolymeric nanocapsules 显示文摘 | Pranee Lertsutthiwong Pornchai Rojsitthisak Ubonthip Nimmannit | 2009 | Materials Science and Engineering C2009，，29： | 1 |
| 11 | Nitric oxide regulates cell sensitivity to Cisplatin-induced apoptosis through S-Nitrosylation and inhibition of Bcl-2 ubiquitination显示文摘 | Chanvorachote P Nimmannit U Stehlik C | 2006 | Cancer Res2006，66，12： | 1 |
| 12 | Ni- tric oxide regulates lung carcinoma cellanoikis through in- hibition of ubiquitin-proteasomal degradation of caveolin- 1 显示文摘 | CHANVORACHOTE P NIMMANNIT U LU Y | 2009 | J Biol Chem2009，284，28： | 1 |
| 13 | Microemulsions as topical delivery vehicles for theanti-melanoma prodrug,temozolomide hexyl ester(TMZA-HE)显示文摘 | Suppasansatom P Nimmannit U Conway B R | 2007 | J Pharm Pharmacol2007，59，6： | 1 |
| 14 | Mitochondrial superoxide mediates doxorubicin-induced keratinocyte apoptosis through oxidative modification of ERK and Bcl-2 ubiquitination显示文摘 | Luanpitpong S Chanvorachote P Nimmannit U | 2012 | Biochem Pharmacol2012，83，： | 1 |
| 15 | Lupus nephritis in male: 8- year experience at Siriraj Hospital显示文摘 | Ongajyooth L Shayakul C Pariehatikanond P Nimmannit S Chirawonq P Vanichakarn S | 1994 | Asian Pac J Allerry Immunol1994，12，2： | 1 |
| 16 | Preparation of turmeric oil-loaded chitosan-alginate biopolymeric nanocapsules显示文摘 | Pranee Lertsutthiwong Pornchai Rojsitthisak Ubonthip Nimmannit | | 0，，： | 1 |
| 17 | Arrhythmogenic marker for the sudden unexplained death syndrome in Thai men显示文摘 | Veerakul G Nimmannit S | 1997 | Circulation1997，96，： | 1 |
| 18 | Preparation of tnrmericoil-loaded chitosan-alginate biopolymerienanocapsules显示文摘 | PRANCE LERTSUTTHIWONG POMCHAIGO JSITTHISAK UBONTHIP NIMMANNIT | 2009 | Materials Science and Engineering C2009，，29： | 1 |
| 19 | Curcumin sensitizes non-small cell lung cancer cell anoikis through reactive oxygen species-mediated Bcl-2 downregulation显示文摘 | Pongrakhananon V Nimmannit U Luanpitpong S | | 0，，： | 1 |
| 20 | Arrhythmogen ic marker for the sudden unexplained death syndrome in Thai men 显示文摘 | Nademanee K Veerakul G Nimmannit S | 2009 | Circulation2009，96，： | 1 |
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