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| 1 | Flavonoids kaempferide and 4,2’-dihydroxy-4’,5’,6’-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells显示文摘Objective: Kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone (DTMC) are two major flavonoids found in Chromolaena odoraia Linn, leaf extract. The aim of this study was to elucidate the mechanism by which these two flavonoids exerted their effect on adipogenesis. The inhibitory effect of kaempferide and DTMC on adipocyte differentiation and their mechanisms involving mitotic clonal expansion (MCE) and apoptosis during the early stage of adipogenesis were investigated. Methods: Confluent 3T3-L1 preadipocytes were induced to differentiate and exposed to the flavonoids during various phases of difTerentiaiion. Intracellular lipid accumulation, cell density and expression of the transcription factors peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding pro-teins a were assessed using AdipoRed, Oil red 0 and Western blot assays. Effects of both flavonoids on cell proliferation and apoptosis were also determined by carboxyfluorescein diacetate succinimidyl ester and annexin V-fluorescein isothiocyanate/propidium iodide-staining assays, respectively. Results: Kaempferide and DTMC showed significant, concentration-dependent anti-adipogenic activity and effect on cell density in the early phase of adipogenesis. The expression of the transcription factors seemed to be reduced when the treatment was prolonged or in the early phase of adipogenesis. These flavonoids interrupted MCE via inhibition of preadipocyte proliferation and induction of apoptosis. DTMC was nearly three times more potent than kaempferide in inducing apoptosis. Conclusion: Kaempferide and DTMC exerted their anti-adipogenic activity through inhibition of MCE, either by suppressing cell proliferation or by inducing apoptosis during the early phase of differentiation. | Supakanya Kumkarnjana Rutt Suttisri Ubonthip Nimmannit Apirada Sucontphunt Mattaka Khongkow Thongchai Koobkokkruad Nontima Vardhanabhuti | 2019 | Journal of Integrative Medicine2019，17，4： | 0 |
| 2 | 川木瓜醇提取物对3T3-L1前脂肪细胞增殖及其分化细胞的影响显示文摘目的观察川木瓜醇提取物对3T3-L1前脂肪细胞增殖及其分化细胞的影响。方法提取制备川木瓜醇提取物;将提取物作用于3T3-L1前脂肪细胞,检测细胞活力变化。通过刺激剂诱导3T3-L1前脂肪细胞,诱变为脂肪细胞,并使用提取物干预,观察细胞形态和细胞活力情况;以及细胞外上清液中葡萄糖含量变化。结果该提取物(25.0~200.0ng/L)能降低3T3-L1前脂肪细胞。提取物(100.0~200.0ng/L)可降低3T3-L1前脂肪细胞分化细胞活力;可使分化细胞悬浮,并明显聚缩为球形;且细胞外上清中葡萄糖含量明显升高。结论川木瓜醇提取物可显著抑制3T3-L1前脂肪细胞和分化的脂肪细胞,致使细胞糖吸收降低,细胞外上清液中葡萄糖含量升高,为川木瓜具有消脂作用提供了初步的理论依据。 | 陆飞燕 姜艳 潘冬 张钰婷 李容 | 2018 | 右江民族医学院学报2018，40，6： | 0 |
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