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| 1 | 转录因子RORγt对妊娠期哮喘模型小鼠Th17/Treg平衡的调节作用显示文摘目的观察妊娠期哮喘模型小鼠Th17细胞相关细胞因子和维甲酸相关孤儿受体γt(RORγt)的表达变化,探讨地塞米松(DXM)对小鼠气道炎症和Th17细胞分泌功能的影响。方法 30只雌性BALB/c小鼠随机分为健康妊娠(HP)组、妊娠期哮喘(AP)组和地塞米松(DXM)组,每组10只。AP组和DXM组于实验当天(0d)和7、14d时分别给予OVA/Al(OH)3混悬液腹腔注射致敏,HP组仅给予单纯等量生理盐水(NS)。3组小鼠合笼交配至妊娠成功。于21d起,连续7d对AP组和DXM组给予50g/L OVA雾化激发(1次/d,40min/次);DXM组于雾化吸入OVA前30min给予1mg/kgDXM腹腔注射;HP组仅给予单纯等量NS进行雾化激发。各组小鼠于末次激发24h后进行气道反应性检测,结果以相应浓度乙酰胆碱(Mch)激发下增强的呼吸间歇(Penh)值与基线值的百分比[Penh值(%)]来表示;分析支气管肺泡灌洗液(BALF)中白细胞总数及分类计数;取肺组织行病理学观察;采用流式细胞仪检测外周血Th17和Treg细胞的比例并计算Th17/Treg比值;ELISA法测定外周血IL-17、IL-23、IFN-γ和RORγt含量,RT-PCR法检测前述因子在肺组织中的表达,Western blotting检测肺组织中RORγt的表达。结果雌性BALB/c小鼠以OVA致敏后气道反应性增加,BALF中白细胞总数及嗜酸性粒细胞(Eos)、淋巴细胞(Lym)和中性粒细胞(Neu)比例增加,肺组织病理学改变符合哮喘的特征。外周血标本中,AP组Th17细胞显著升高,Treg细胞显著降低(P<0.05),Th17/Treg比值上升。DXM组Th17和Treg细胞变化趋势与AP组相同,Th17/Treg比值高于HP组,但低于AP组(P<0.05)。分别在肺组织和外周血标本中检测蛋白和mRNA表达水平,结果显示,AP组IL-17、IL-23及RORγt含量均高于HP组及DXM组(P<0.05),IFN-γ含量低于HP组及DXM组(P<0.05)。AP组肺组织RORγt蛋白的表达(61.27%±3.11%)明显高于DXM组(37.51%±1.93%,P<0.01)和HP组(22.39%±0.86%,P<0.01),后两者比较差异亦有统计学意义(P<0.01)。相关分析表明,AP组肺组织RORγt蛋白表达量与Eos、Lym和Neu细胞绝对值呈显著正相关(r=0.879、0.759、0.802,P<0.01),与外周血IL-17含量、肺组织IL-17mRNA表达水平亦呈正相关(r=0.696、0.863,P<0.01)。结论 Th17细胞分泌功能及RORγt表达上调参与了妊娠期哮喘的发生发展。DXM干预在改善哮喘症状的同时可降低RORγt表达,使Th17分化和增殖功能受抑。 | 马佳佳 Nick Lu 陈必良 | 2012 | 解放军医学杂志2012，37，6： | 20 |
| 2 | Resistance status of the carmine spider mite, Tetranychus cinnabarinus and the twospotted spider mite, Tetranychus urticae to selected acaricides on strawberries显示文摘洋红蜘蛛螨, Tetranychus cinnabarinus (Boisduval ) 和 twospotted 搜寻螨, Tetranychus urticae Koch,是草莓和许多另外的园艺的庄稼的严肃的害虫。这些害虫的控制重重地依赖于化学杀螨剂。这研究的目的是决定这的抵抗地位二害虫种到在一个整年的集中的园艺的生产区域的草莓上的通常使用的杀螨剂。而那为成年 twospotted 蜘蛛螨是 24% 最高的标签率,为成年洋红蜘蛛螨的 abamectin 的 LC 90 是 4% 。spiromesifen, etoxazole, hexythiazox 和 bifenazate 的 LC 90s 是为洋红蜘蛛螨鸡蛋的 0.5% 他们的各自的最高的标签率, 0.5% , 1.4% 和 83% ,为美女的 0.7% 他们的各自的最高的标签, 2.7% , 12.1% 和 347% 率。spiromesifen, etoxazole, hexythiazox 和 bifenazate 的 LC 90s 是为 twospotted 蜘蛛螨鸡蛋的 4.6% 他们的各自的最高的标签率, 11.1% , 310% 和 62% ,为美女的 3% 他们的各自的最高的标签, 13% , 432,214% 和 15% 率。我们的结果建议那 T。cinnabarinus 开发了抵抗到 bifenazate 并且 T。urticae 开发了抵抗到 hexythiazox。这些结果强烈强调需要在这个区域开发抵抗管理策略。 | Jian-Long Bi Zi-Mian Niu Lu Yu Nick C. Toscano | 2016 | Insect Science2016，23，1： | 7 |
| 3 | The Seasonal Prediction of the Exceptional Yangtze River Rainfall in Summer 2020显示文摘During June and July of 2020,the Yangtze River basin suffered from extreme mei-yu rainfall and catastrophic flooding.This study explores the seasonal predictability and associated dynamical causes for this extreme Yangtze River rainfall event,based on forecasts from the Met Office GloSea5 operational forecast system.The forecasts successfully predicted above-average rainfall over the Yangtze River basin,which arose from the successful reproduction of the anomalous western North Pacific subtropical high(WNPSH).Our results indicate that both the Indian Ocean warm sea surface temperature(SST)and local WNP SST gradient were responsible for the westward extension of the WNPSH,and the forecasts captured these tropical signals well.We explore extratropical drivers but find a large model spread among the forecast members regarding the meridional displacements of the East Asian mid-latitude westerly jet(EAJ).The forecast members with an evident southward displacement of the EAJ favored more extreme Yangtze River rainfall.However,the forecast Yangtze River rainfall anomaly was weaker compared to that was observed and no member showed such strong rainfall.In observations,the EAJ displayed an evident acceleration in summer 2020,which could lead to a significant wind convergence in the lower troposphere around the Yangtze River basin,and favor more mei-yu rainfall.The model forecast failed to satisfactorily reproduce these processes.This difference implies that the observed enhancement of the EAJ intensity gave a large boost to the Yangtze River rainfall,hindering a better forecast of the intensity of the event and disaster mitigation. | Chaofan LI Riyu LU Nick DUNSTONE Adam ASCAIFE Philip EBETT Fei ZHENG | 2021 | Advances in Atmospheric Sciences2021，38，12： | 5 |
| 4 | 表达白介素-23基因的重组纯化及其对妊娠期哮喘气道炎症状态下免疫应答产生的影响显示文摘目的研究IL-23融合基因免疫调节及其生物学功能与妊娠期哮喘气道炎症的相关性。方法用构建表达IL-23p40+p19双亚基真核表达质粒转化E.coil BL21菌,IPTG诱导表达产物纯化后,通过SDS-PAGE和蛋白质印迹法进行检测;荧光显微镜及Western blot法鉴定重组蛋白介导质粒转染结果,凝胶电泳迁移实验测定核蛋白的DNA结合活性变化;ELISA法、HE染色分析外源蛋白体内转移对小鼠血清炎性因子水平及肺组织炎症损伤影响;流式细胞术验证外周血单个核细胞中CD4+T细胞亚群比例。结果成功获得可溶性重组外源蛋白可跨膜转运,并有效介导EGFP进入目标细胞,其稳定表达及转录活性均高于单纯质粒转染效应;GST/pCEP4-IL-23特异结合质粒DNA形成大分子复合体使凝胶迁移率降低;与LPS共同作用,明显增加致敏小鼠血清IL-17A含量(P<0.05)和肺组织炎症病理反应程度;降低患者外周血IFN-γ、Foxp3水平,促进IL-4、IL-17A活化,进而升高Th2/Th1和Th17/Treg比值(P<0.05)。结论重组IL-23蛋白具有致病性,参与妊娠哮喘炎症调控,CD4+T亚群免疫失衡共同对妊娠母体免疫耐受及疾病发生发展产生影响。 | 马佳佳 Nick Lu 陈必良 | 2014 | 中国药理学通报2014，30，3： | 4 |
| 5 | CD4^+T细胞亚群表达失衡在妊娠期哮喘发病中的作用及意义显示文摘目的:观察妊娠期哮喘小鼠模型中Th17及Treg细胞活性分布和表达变化,探讨Th17/Treg细胞免疫平衡在妊娠期哮喘小鼠发病中的作用机制。方法:24只6~8周龄,雌性BALB/c小鼠随机分为4组,每组6只。以OVA/Al(OH)3混悬液致敏与激发构建妊娠期哮喘组(AP组)和非妊娠期哮喘组(ANP组)小鼠模型;健康妊娠组(HP组)和健康非妊娠组(HNP组)小鼠致敏与激发均以只含Al(OH)3的生理盐水混悬液替代。光镜下观察小鼠支气管肺泡灌洗液(BALF)中细胞计数及分类;HE染色评价小鼠气道炎症程度及肺组织病理学变化;流式细胞术测定小鼠外周血Th17及Treg细胞所占CD4+T细胞比例,计算Th17/Treg细胞比值;RT-PCR检测小鼠肺组织中Th17及Treg细胞相关因子表达。结果:妊娠期哮喘小鼠BALF中炎症细胞分类及计数、肺组织病理学表现和活体肺功能气道高反应性Penh值等客观指标证实,妊娠哮喘小鼠模型构建成功。外周血中,AP组CD4+IL-17+T细胞(Th17)显著升高(P<0.01);Foxp3+T细胞(Treg)显著降低(P<0.01);与HP,HNP组相比,存在Th17/Treg比例失衡(P<0.01)。AP组小鼠肺组织中IL-17、IL-23,IL-10 mRNA表达水平均显著高于HP组及HNP组(P<0.01);IFN-γmRNA则显著低于HP组及HNP组(P<0.01);ANP组各因子mRNA水平变化与AP组一致,组间无显著差异。结论:妊娠期哮喘小鼠存在细胞免疫功能失调;Th17、Treg细胞数量及免疫平衡状态发生改变,可能是妊娠期哮喘发病过程中一个重要的决定因素。 | 马佳佳 Nick Lu 陈必良 | 2012 | 现代妇产科进展2012，21，9： | 3 |
| 6 | Seasonal Forecasts of the Summer 2016 Yangtze River Basin Rainfall显示文摘The Yangtze River has been subject to heavy flooding throughout history,and in recent times severe floods such as those in 1998 have resulted in heavy loss of life and livelihoods.Dams along the river help to manage flood waters,and are important sources of electricity for the region.Being able to forecast high-impact events at long lead times therefore has enormous potential benefit.Recent improvements in seasonal forecasting mean that dynamical climate models can start to be used directly for operational services.The teleconnection from El Ni ?no to Yangtze River basin rainfall meant that the strong El Ni ?no in winter 2015/16 provided a valuable opportunity to test the application of a dynamical forecast system.This paper therefore presents a case study of a real-time seasonal forecast for the Yangtze River basin,building on previous work demonstrating the retrospective skill of such a forecast.A simple forecasting methodology is presented,in which the forecast probabilities are derived from the historical relationship between hindcast and observations.Its performance for2016 is discussed.The heavy rainfall in the May–June–July period was correctly forecast well in advance.August saw anomalously low rainfall,and the forecasts for the June–July–August period correctly showed closer to average levels.The forecasts contributed to the confidence of decision-makers across the Yangtze River basin.Trials of climate services such as this help to promote appropriate use of seasonal forecasts,and highlight areas for future improvements. | Philip E. BETT Adam A. SCAIFE Chaofan LI Chris HEWITT Nicola GOLDING Peiqun ZHANG Nick DUNSTONE Doug M. SMITH Hazel E. THORNTON Riyu LU Hong-Li REN | 2018 | Advances in Atmospheric Sciences2018，35，8： | 3 |
| 7 | Virtual vignettes: the acquisition,analysis,and presentation of social network data显示文摘Online social networks(OSNs)are immensely prevalent and have now become a ubiquitous and important part of the modern,developed society.However,online social networks pose significant problems to digital forensic investigators who have no experience online.Data will reside on multiples of servers in multiple countries,across multiple jurisdictions.Capturing it before it is overwritten or deleted is a known problem,mirrored in other cloud based services.In this article,a novel method has been developed for the extraction,analysis,visualization,and comparison of snapshotted user profile data from the online social network Twitter.The research follows a process of design,implementation,simulation,and experimentation.Source code of the tool that was developed to facilitate data extraction has been made available on the Internet. | HOWDEN Chris LIU Lu LI ZhiYuan LI JianXin ANTONOPOULOS Nick | 2014 | Science China(Information Sciences)2014，57，3： | 1 |
| 8 | Translational Regulatory Mechanisms Generate N-Terminal Glucocorticoid Receptor Isoforms with Unique Transcriptional Target Genes显示文摘 | Nick Z. Lu John A. Cidlowski | 2005 | Molecular Cell2005，，3： | 1 |
| 9 | IL-17R-FcγRⅡb基因体外诱导树突状细胞表达及其对淋巴细胞增殖活化的影响显示文摘目的:构建编码人全长IL-17R与FcγRⅡb双基因表达重组腺病毒载体并对其特性进行鉴定,探讨阻断其信号调节通路对妊娠哮喘发病的影响。方法:将扩增出的拼接基因片段定向克隆于pAdTrack-CMV中经与pAdeasy-1同源重组,鉴定正确的阳性重组子通过脂质体介导转入AD293细胞,荧光显微镜观察绿色荧光(GFP)信号并采用PCR法对其进行鉴定;纯化腺病毒感染体外细胞因子协同诱导培养的妊娠哮喘模型小鼠骨髓来源树突状细胞(DC),利用RT-PCR、Western免疫印迹检测IL-17R和FcγRⅡb表达;流式细胞术、CCK-8、ELISA分析重组腺病毒的修饰对DC表面分子表达,刺激T细胞增殖抑制变化和MLR上清中IFN-γ、IL-4、IL-17分泌水平影响。结果:构建的重组腺病毒载体插入序列完全正确;经GFP表达和PCR检测证实成功包装获得高滴度重组腺病毒;感染DCs的GFP阳性细胞数达到85%;在mRNA及蛋白水平均可同时转录和表达出符合预期的IL-17R和FcγRⅡb。CD11c阳性表达率在各组间无显著差异;与对照细胞及EGFP-DCs相比,经IL-17RFcγRⅡb修饰的DC表面CD80表达增高,而CD86表达减少(P<0.05);IL-17R-FcγRⅡb-DCs相同浓度比例刺激T细胞增殖水平均明显受抑(P<0.05);转染载体共培养物上清液中Th2/Th17类细胞因子IL-4、IL-17水平下降,Th1类细胞因子IFN-γ升高(P<0.05),IFN-γ/IL-4/IL-17相对比值增加(P<0.05)。结论:IL-17R-FcγRⅡb融合基因体外阻断CD86/CD28共刺激通路可诱导DC免疫耐受状态,抑制抗原特异性T细胞增殖并影响Th1/Th2/Th17亚群分化,有望在妊娠哮喘免疫耐受缺陷和缓解气道炎症反应中发挥作用。 | 马佳佳 Nick Lu 陈必良 | 2013 | 中国免疫学杂志2013，29，11： | 1 |
| 10 | 重组IL-12表达载体构建及对妊娠期哮喘小鼠气道炎症的调节与免疫应答影响显示文摘目的:构建携带IL-12重组基因的真核表达载体,探讨其对妊娠期哮喘小鼠气道炎症的免疫调节作用及其可能机制。方法:PCR扩增mIL-12基因cDNA,利用DNA重组技术将线性化片段定向插入载体pcDNA3.1(+)质粒中,经酶切和测序鉴定,通过脂质体介导瞬时转染P815细胞,观察荧光蛋白在细胞中的表达和定位。以鸡卵清蛋白(OVA)致敏和激发建立妊娠期哮喘小鼠模型,通过气道内灌注:妊娠期哮喘(AP)+pcDNA3.1(+)-mIL-12组(AP1组)、单纯哮喘(ANP)+pcD-NA3.1(+)-mIL-12组(ANP1组)给予50μl pcDNA3.1(+)-mIL-12/脂质体混合液;AP+pcDNA3.1(+)组(AP2组)、ANP+pcDNA3.1(+)组(ANP2组)给予50μl pcDNA3.1(+)/脂质体混合液;单纯妊娠组(HP组)、阴性对照组(HNP组)给予50μlPBS。于末次激发24小时后,观察肺泡灌洗液(BALF)炎性细胞计数及肺组织HE染色以评价小鼠气道炎症程度;RT-PCR、Western blot鉴定小鼠肺组织匀浆中IL-12蛋白及mRNA表达;流式细胞术检测小鼠脾细胞IL-17+及IFN-γ+分泌水平;ELISA测定小鼠外周血上清中细胞因子含量变化。结果:成功构建重组pcDNA3.1(+)-mIL-12真核表达载体,并在P815细胞中获得高效表达。与HP、HNP组相比,AP1、ANP1组BALF中白细胞总数及Eos,Neu,Lym占细胞总数百分比均显著低于AP2、ANP2组,但高于HP和HNP组,AP1与ANP1组间尤以AP1组降低显著(P<0.05),且肺组织炎症细胞浸润明显减轻,炎症反应明显轻微。肺组织标本中,AP1、ANP1组IL-12蛋白含量及mRNA表达水平在重组质粒干预后显著高于AP2、ANP2组。脾组织标本中AP1、ANP1组IFN-γ+含量明显升高,而CD4+IL-17+明显降低(P<0.05);IFN-γ+与CD4+IL-17+比例高于AP2、ANP2组(P<0.05);此变化趋势AP1组比ANP1组更为明显(P<0.05);HP组IFN-γ+及IL-17+含量变化与HNP组组间差异不显著。外周血上清中细胞因子检测结果 AP1、ANP1组IFN-γ明显升高,IL-4、IL-17显著降低(P<0.05)。结论:构建的pcD-NA3.1(+)-mIL-12真核表达载体能够在明显改善妊娠期哮喘小鼠气道炎症反应的同时上调IFN-γ表达,使Th2、Th17型细胞分化和增殖功能受抑,证实IL-12表达上调参与了妊娠期哮喘的发生发展。 | 马佳佳 Nick Lu 陈必良 滑玮 张建芳 | 2013 | 中国免疫学杂志2013，29，3： | 0 |
| 11 | Acute hepatitis of unknown aetiology among children around the world显示文摘By 26 August 2022, the number of cases of acute hepatitis of unknown etiology (AHUA) has drastically increased to 1115 distributed in 35 countries that fulfill the World Health Organization definition. Several hypotheses on the cause of AHUA have been proposed and are being investigated around the world. In the recent United Kingdom (UK) report, human adenovirus (HAdV) with adeno-associated virus (AAV) co-infection is the leading hypothesis. However, there is still limited evidence in establishing the causal relationship between AHUA and any potential aetiology. The leading aetiology continues to be HAdV infection. It is reported that HAdV genomics is not unusual among the population in the UK, especially among AUHA cases. Expanding the surveillance of HAdV and AAV in the population and the environment in the countries with AUHA cases is suggested to be the primary action. Metagenomics should be used in detecting other infectious pathogens on a larger scale, to supplement the detection of viruses in the blood, stool, and liver specimens from AUHA cases. It is useful to develop a consensus-specific case definition of AHUA to better understand the characteristics of these cases globally based on all the collected cases. | Chao Wang Zhi‑Yong Gao Nick Walsh Stephen Hadler Qing‑Bin Lu Fuqiang Cui | 2022 | Infectious Diseases of Poverty2022，11，6： | 0 |
| 12 | 人可溶性sTNFR1及胞膜外区Ig融合基因重组腺病毒的真核表达及功能活性鉴定显示文摘目的构建携带人sTNFR1与IgGFc片段融合基因的重组腺病毒,稳定表达并初步鉴定其功能活性在妊娠哮喘炎症机制中的作用。方法将PCR扩增的sTNFR1及IgGFc基因片段先后连接到穿梭质粒pAdTrack-CMV多克隆位点上,酶切线性化后在BJ5183细菌内与骨架质粒pAdEasy-1同源重组,产生获得的AdE-EGFP-sTNFR1-Ig以脂质体法转染AD293细胞进行病毒包装,以绿色荧光蛋白(GFP)监测病毒滴度及感染效率。RT-PCR、Western blot实验鉴定细胞内重组腺病毒转录及融合基因与蛋白表达;流式细胞术、ELISA实验分析混合培养上清中转染肥大细胞(MC)激活、调节同基因来源T细胞表达Th2/Treg水平及细胞因子分泌效果;MTT评估转染细胞上清中sTNFR1-Ig对TNF-α介导细胞毒效应的拮抗作用。结果成功构建编码sTNFR1-Ig基因的重组腺病毒载体经酶切和测序鉴定正确,制备的腺病毒在转染细胞中的GFP表达以及CPE效应明显,扩增后的滴度为3.7×1010~4.8×1010pfu/ml,体外感染效率可达90%以上;sTNFR1-Ig基因在mRNA及蛋白水平获得的清晰条带均证实腺病毒包装与扩增成功。表达sTNFR1-Ig的MC诱导T细胞转化为CD4+CD25+Foxp3+T细胞水平以及IL-12含量均明显升高,CD4+IL-4+Th2细胞水平下调(P<0.05),Th2/Treg所占CD4+T细胞比值下降(P<0.05)。sTNFR1-Ig封闭TNF-α对MC免疫毒性作用的浓度效应明显,而EGFP-MCs与对照细胞上清对TNF-α毒性效应的中和阻断能力不足(P<0.05)。结论携sTNFR1-Ig基因重组腺病毒参与的免疫调控与妊娠期哮喘发生发展密切相关。sTNFR1及IgG-Fc亚基胞膜外区结合的表达和引起免疫耐受的作用研究,为妊娠哮喘发病机制在理论上的新突破,为以sTNFRI-Ig为靶点的妊娠哮喘干预治疗奠定基础。 | 马佳佳 Nick Lu 陈必良 | 2013 | 中国药理学通报2013，29，8： | 0 |
| 13 | Prediction of the Western North Pacific Subtropical High in Summer without Strong ENSO Forcing显示文摘The western North Pacific subtropical high(WNPSH) is one of the deterministic predictors of the East Asian summer climate, and a better prediction of the WNPSH favors more reasonable forecast of the East Asian summer climate. This study focuses on seasonal prediction of the WNPSH during neutral summers without strong El Ni?o–Southern Oscillation(ENSO) forcing, and explores the associated predictable sources, using the one-month lead time retrospective forecasts from the Ensembles-Based Predictions of Climate Changes and Their Impacts(ENSEMBLES) project during 1960–2005. The results indicate that the ENSEMBLES atmosphere–ocean–land coupled models exhibit considerable prediction skill for the WNPSH during neutral summers, with successful reproduction of the WNPSH in the majority of neutral summers. The anomalous WNPSH in neutral summers, which corresponds to cyclonic/anticyclonic anomalies in the lower troposphere, is highly correlated with an east–west dipole local sea surface temperature(SST) distribution over the tropical WNP, suggesting an intimate local air–sea coupling. Further diagnosis of the local SST–rainfall relationship and surface heat flux indicates that the anomalous local SST plays an active role in modulating the variation of the WNPSH during neutral summers, rather than passively responding to the atmospheric change. The local SST anomalies and relevant air–sea coupling over the tropical WNP are reasonably well reproduced in the model predictions, and could act as primary predictable sources of the WNPSH in neutral summers. This could aid in forecasting of the East Asian rainband and associated disaster mitigation planning. | Chaofan LI Riyu LU Nick DUNSTONE | 2021 | Journal of Meteorological Research2021，35，1： | 0 |
| 14 | A Survey on Event Tracking in Social Media Data Streams显示文摘Social networks are inevitable parts of our daily life,where an unprecedented amount of complex data corresponding to a diverse range of applications are generated.As such,it is imperative to conduct research on social events and patterns from the perspectives of conventional sociology to optimize services that originate from social networks.Event tracking in social networks finds various applications,such as network security and societal governance,which involves analyzing data generated by user groups on social networks in real time.Moreover,as deep learning techniques continue to advance and make important breakthroughs in various fields,researchers are using this technology to progressively optimize the effectiveness of Event Detection(ED)and tracking algorithms.In this regard,this paper presents an in-depth comprehensive review of the concept and methods involved in ED and tracking in social networks.We introduce mainstream event tracking methods,which involve three primary technical steps:ED,event propagation,and event evolution.Finally,we introduce benchmark datasets and evaluation metrics for ED and tracking,which allow comparative analysis on the performance of mainstream methods.Finally,we present a comprehensive analysis of the main research findings and existing limitations in this field,as well as future research prospects and challenges. | Zixuan Han Leilei Shi Lu Liu Liang Jiang Jiawei Fang Fanyuan Lin Jinjuan Zhang John Panneerselvam Nick Antonopoulos | 2024 | Big Data Mining and Analytics2024，7，1： | 0 |
| 15 | 靶向沉默Gata3对妊娠期哮喘小鼠树突状细胞表型及效应T细胞失衡表达的影响显示文摘目的:研究慢病毒介导siRNA靶向沉默Gata3基因表达对妊娠哮喘模型小鼠骨髓来源树突状细胞(DC)抗原递呈及其免疫生物学特性的影响。方法:以脂质体DNA沉淀法将重组慢病毒干扰质粒与包装质粒共转染AD293细胞,包装生成慢病毒感染体外诱导DC,RT-PCR、Western blot分析其对siGata3基因的整合转录效果;流式细胞术观察细胞表型变化。通过尾静脉注射将pSH1/siGata3转移至小鼠体内,ELISA法测定其肺泡灌洗液(BALF)中的炎性因子含量;MTT法验证混合淋巴细胞反应(MLR)后T细胞增殖能力。结果:经重组、包装携有Gata3特异siRNA慢病毒感染DC成功,其siRNA靶点Gata3 mRNA及蛋白的转录表达分别下调87.30%和81.33%(P<0.05);同时降低DCs膜表面CD86荧光强度(P<0.05)。减少pSH1/siGata3组小鼠BALF中IL-5、IL-17分泌而提升IL-12水平(P<0.05);MLR证实,共培养感染DC激活T细胞的能力明显不足(P<0.05)。结论:siRNA干扰可有效抑制模型小鼠免疫微环境特定Gata3通路,阻遏T细胞及其亚群分化由此来诱导DC免疫耐受,为妊娠期哮喘防治奠定基础并提供新的思路和手段。 | 马佳佳 陈必良 Nick Lu | 2014 | 现代妇产科进展2014，23，7： | 0 |
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