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| 1 | A randomized double-blind trial on perioperative administration of probiotics in colorectal cancer patients显示文摘AIM:To investigate whether probiotic bacteria,given perioperatively,might adhere to the colonic mucosa, reduce concentration of pathogens in stools,and modulate the local immune function. METHODS:A randomized,double-blind clinical trial was carried out in 31 subjects undergoing elective colorectal resection for cancer.Patients were allocated to receive either a placebo(group A,n=10),or a dose of 10 7 of a mixture of Bifidobacterium longum(BB536) and Lactobacillus johnsonii(La1)(group B,n=11),or the same mixture at a concentration of 10 9 (group C,n=10).Probiotics,or a placebo,were given orally 2 doses/d for 3 d before operation.The same treatment continued postoperatively from day two to day four. Stools were collected before treatment,during surgery (day 0)and 5 d after operation.During the operation, colonic mucosa samples were harvested to evaluate bacterial adherence and to assess the phenotype of dendritic cells(DCs)and lymphocyte subsets by surface antigen expression(flow cytometry).The presence of BB536 and La1 was evaluated by the random amplified polymorphism DNA method with specific polymerase chain reaction probes. RESULTS:The three groups were balanced for baseline and surgical parameters.BB536 was never found at any time-points studied.At day 0,La1 was present in 6/10(60%)patients in either stools or by biopsy in group C,in 3/11(27.2%)in group B,and none in the placebo group(P=0.02,C vs A).There was a linear correlation between dose given and number of adher- ent La1(P=0.01).The rate of mucosal colonization by enterobacteriacae was 30%(3/10)in C,81.8%(9/11) in B and 70%(7/10)in A(P=0.03,C vs B).The Enterobacteriacae count in stools was 2.4(log10 scale) in C,4.6 in B,and 4.5 in A(P=0.07,C vs A and B). The same trend was observed for colonizing enterococ- ci.La1 was not found at day+5.We observed greater expression of CD3,CD4,CD8,and naive and memory lymphocyte subsets in group C than in group A with a dose response trend(C>B>A).Treatment didnot affect DC phenotype or activation,but after ex vivo stimulation with lipopolysaccharides,groups C and B had a lower proliferation rate compared to group A (P=0.04).Moreover,dendritic phenotypes CD83-123, CD83-HLADR,and CD83-11c(markers of activation) were significantly less expressed in patients colonized with La1(P=0.03 vs not colonized). CONCLUSION:La1,but not BB536,adheres to the colonic mucosa,and affects intestinal microbiota byreducing the concentration of pathogens and modulates local immunity. | Luca Gianotti Lorenzo Morelli Francesca Galbiati Simona Rocchetti Sara Coppola Aldo Beneduce Cristina Gilardini Daniela Zonenschain Angelo Nespoli Marco Braga | 2010 | World Journal of Gastroenterology2010，16，2： | 18 |
| 2 | Hepatic resection beyond barcelona clinic liver cancer indication:When and how显示文摘Hepatocellular carcinoma(HCC)is the main common primary tumour of the liver and it is usually associated with cirrhosis.The barcelona clinic liver cancer(BCLC)classification has been approved as guidance for HCC treatment algorithms by the European Association for the Study of Liver and the American Association for the Study of Liver Disease.According to this algorithm,hepatic resection should be performed only in patients with small single tumours of 2-3 cm without signs of portal hypertension(PHT)or hyperbilirubinemia.BCLC classification has been criticised and many studies have shown that multiple tumors and large tumors,as wide as those with macrovascular infiltration and PHT,could benefit from liver resection.Consequently,treatment guidelines should be revised and patients with intermediate/advanced stage HCC,when technically resectable,should receive the opportunity to be treated with radical surgical treatment.Nevertheless,the surgical treatment of HCC on cirrhosis is complex:The goal to be oncologically radical has always to be balanced with the necessity to minimize organ damage.The aim of this review was to analyze when and how liver resection could be indicated beyond BCLC indication.In particular,the role of multidisciplinary approach to assure a proper indication,of the intraoperative ultrasound for intraoperative restaging and resection guidance and of laparoscopy to minimize surgical trauma have been enhanced. | Mattia Garancini Enrico Pinotti Stefano Nespoli Fabrizio Romano Luca Gianotti Vittorio Giardini | 2016 | World Journal of Hepatology2016，8，11： | 8 |
| 3 | Silent myocardial ischemia in diabetic and nondiabetic patients with coronary artery disease显示文摘 | C. Falcone L. Nespoli D. Geroldi C. Gazzaruso M.P. Buzzi C. Auguadro L. Tavazzi P.J. Schwartz | 2002 | International Journal of Cardiology2002，，2： | 1 |
| 4 | A prospective study of recurrence rate and risk factors for recurrence after a first renal stone显示文摘 | Trinehieri A Ostini F Nespoli R | 1999 | J Urol1999，162，1： | 1 |
| 5 | Silent myocardial ischemia in diabetic and nondiabetic patients with coronary artery disease显示文摘 | Falcone C Nespoli L Geroldi D | 2003 | Int J Cardiol2003，90，23： | 1 |
| 6 | Encephalopathy,oxygen consumption visceral amino acid clearance, and mortality in cirrhotic surgical patients 显示文摘 | Load M Clowcs GH Nespoli A | 1984 | Am J Sury1984，147，4： | 1 |
| 7 | A randomized controlled trial of preoperative oral supplementation with a specialized diet in patients with gastrointestinal cancer显示文摘 | Braga M Nespoli L | 2002 | Gastroenterology2002，122，7： | 1 |
| 8 | Hopf bifurcation from lines of equilibria without parameters in memristors oscillators显示文摘 | Messias M Nespoli C Botta V A | | 0，，2： | 1 |
| 9 | Nutritional approach in malnourished surgical patients: a prospective randomized study显示文摘 | Gianotti L Nespoli L | 2002 | Arch Surg2002，137，2： | 1 |
| 10 | Silent myocardial ischemia in diabet-ic and nondiabetic patients with coronary artery disease显示文摘 | Falcone C Nespoli L Geroli D | 2003 | Int JCardiol2003，8，： | 1 |
| 11 | Nutritional therapy in surgical patients:an update显示文摘 | Gianotti L Nespoli L Sargenti M | 2003 | Minerva Anestesiol2003，69，4： | 1 |
| 12 | Gut oxygenation and oxidative damage during and after laparoscopic and open left-sidedcolon resection:a prospective,randomized,controlled clinical trial显示文摘 | Gianotti L Nespoli L Rocchetti S | 2011 | Surg Endosc2011，25，6： | 1 |
| 13 | Role of chest radiography in the diagnosis of allergic bronchopulmonary aspergillosis in adult patients with cystic fibrosis显示文摘 | G. Cortese V. Malfitana R. Placido A. Ferrari B. Grosso V. Rose P. Nespoli C. Fava | 2007 | La radiologia medica2007，，： | 1 |
| 14 | Nutritional approach in malnourished surgical patients: a prospective randomized study 显示文摘 | Braga M Gianotti L Nespoli L | 2002 | Arch Surg2002，137，2： | 1 |
| 15 | A randomized controlled trial of preoperative oral supplementation with a specialized diet in patients with gastrointestinal cancer显示文摘 | Gianotti L Braga M Nespoli L | 2002 | Gastroenterology2002，122，7： | 1 |
| 16 | Dilent myocardial ischemia in diabe tic and nondiabetic patients with coronary artery discase显示文摘 | Nespoli L Geroldi D | 2003 | IntJ Cardiol2003，90，23： | 1 |
| 17 | Nutritional approach in malnourished surgical patients: a prospective randomized study 显示文摘 | Braga M Gianotti L Nespoli L | 2002 | Arch Surg2002，137，2： | 1 |
| 18 | A randomized controlled trial of preoperative oral supplementation with a specialized diet in patients with gastrointestinal cancer显示文摘 | Luca Gianotti Marco Braga Luca Nespoli Giovanni Radaelli Aldo Beneduce Valerio Di Carlo | 2002 | Gastroenterology2002，，7： | 1 |
| 19 | A randomized con- trolled trial of preoperative oral supplementation with a spe- cialized diet in patients with gastrointestinal cancer 显示文摘 | Gianotti L Braga M Nespoli L | 2002 | Gas- troenterology2002，122，： | 1 |
| 20 | Silent myocardial ischemia in diabetic and nondiabetic patiens with coronary artery disease 显示文摘 | Falcome C Nespoli L Geroldi D | 2003 | Int J Cardiol2003，90，223： | 1 |
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