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| 1 | Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation显示文摘Mesenchymal stem cell(MSC)-based treatments have shown promise for improving tendon healing and repair.MSCs have the potential to differentiate into multiple lineages in response to select chemical and physical stimuli, including into tenocytes. Cell elongation and cytoskeletal tension have been shown to be instrumental to the process of MSC differentiation. Previous studies have shown that inhibition of stress fiber formation leads MSCs to default toward an adipogenic lineage, which suggests that stress fibers are required for MSCs to sense the environmental factors that can induce differentiation into tenocytes. As the Rho/ROCK signal transduction pathway plays a critical role in both stress fiber formation and in cell sensation, we examined whether the activation of this pathway was required when inducing MSC tendon differentiation using rope-like silk scaffolds. To accomplish this, we employed a loss-of-function approach by knocking out ROCK,actin and myosin(two other components of the pathway) using the specific inhibitors Y-27632, Latrunculin A and blebbistatin, respectively. We demonstrated that independently disrupting the cytoskeleton and the Rho/ROCK pathway abolished the expression of tendon differentiation markers and led to a loss of spindle morphology. Together, these studies suggest that the tension that is generated by MSC elongation is essential for MSC teno-differentiation and that the Rho/ROCK pathway is a critical mediator of tendon differentiation on rope-like silk scaffolds. | Edward Maharam Miguel Yaport Nathaniel L Villanueva Takintope Akinyibi Damien Laudier Zhiyong He Daniel J Leong Hui B Sun | 2015 | Bone Research2015，3，3： | 4 |
| 2 | Comparing the parmer drought index and the standardized precipitation index显示文摘 | Nathaniel B Guttman | 1998 | Journal of the American Water Resources Association1998，34，1： | 1 |
| 3 | The role of elevation and soil chemistry in the distribution and ion accumulation of floral morphs of Streptanthus polygaloides Gray (Brassicaceae), a Californian nickel?hyperaccumulator 显示文摘 | Nathaniel P Michael F Robert S B | 2014 | Plant ecology & diversity2014，7，42： | 1 |
| 4 | In- fluences of bending crack and water-cement ratio on chloride-in- duced corrosion of main reinforcing bars and stirrups显示文摘 | Nobuaki Otsuki Shin-iehi Miyazato Nathaniel B Ddiola | 2000 | ACI Ma- terMs Journal2000，97，4： | 1 |
| 5 | Entrepreneurial orientation and market orientation as drivers of product innova- tion success: A study of exporters from a developing economy显示文摘 | Nathaniel B Cadogan J W | 2012 | International Small Business Journal2012，11，3： | 1 |
| 6 | Treatment of Postherpetic Neuralgia:A Review of Therapeutic Options 显示文摘 | Charles EA Nathaniel K Miroslav B | 2004 | J Pain Symptom Manage2004，28，4： | 1 |
| 7 | The Benefits and Cost Impact of AluminumNaval Ship Structure显示文摘 | THOMAS L NATHANIEL B THOMAS I | 2011 | Transactions2011，，119： | 1 |
| 8 | An improved QC process for temperature in the daily cooperative weather obser- vations 显示文摘 | Hubbard K G Nathaniel B G You J S | 2007 | Atmos Oceanic Technical2007，24，： | 1 |
| 9 | Nucleic acid purification using microfabricated silicon structures显示文摘 | Nathaniel C C Scott S Carl A B | 2003 | Biosensors and Bioelectronics2003，19，1： | 1 |
| 10 | Optimal Light Storage in Atomic Vapor 显示文摘 | NATHANIEL PHILLIPS B ALEXEY GORSHKOV V IRINA NOVIKOVA | 2008 | PhysRevA2008，78，02： | 1 |
| 11 | Structural and electronic stability of russian - doll - structured Sc4C2@ C80 显示文摘 | KAH C B NATHANIEL J WANG Xiao - Qian | 2010 | J Phys Chem C2010，114，13： | 1 |
| 12 | Granulocyte-macrophage colony-stimulating factor and interleukin-2 fusion cDNA for cancer gene immunotherapy显示文摘 | John S Jian HW Nathaniel B | 2004 | Cancer Res2004，64，： | 1 |
| 13 | Temperature-driven decoupling of key phases of organic matter degradation in marine sediments 显示文摘 | Nathaniel B Weston Samantha B Joye | 2005 | Proceedings of the National Academy of Sciences2005，102，17： | 1 |
| 14 | NMDA receptor-dependent ocular dominance plasticity in adult visual cortex 显示文摘 | Nathaniel B Sawtell Mikhail Y | 2003 | Neuron2003，38，： | 1 |
| 15 | A new criterion of phase separation : the effect of diamine chain extenders on the properties of polyurethaneureas 显示文摘 | Can B H Robert S W Nathaniel S S | 1982 | Journal of Applied Polymer Science1982，27，6： | 1 |
| 16 | A critical review of the risks to water resources from unconventional shale gas development and hydraulic fracturing in the United States 显示文摘 | AVNER V JACKSON R B NATHANIEL W eta/ | 2014 | Environmental Science & Technology2014，48，15： | 1 |
| 17 | Left Ventricular Hypertrophy: Relationship of Anatomic, Echocardiographic and Electrocardiographic Findings显示文摘 | NATHANIEL REICHEK RICHARD B DEVEREUX | 1981 | Circulation1981，，6： | 1 |
| 18 | Shaowei Chen显示文摘 | Wei Chen Nathaniel B Zuckerman Joseph P Konopelski | 2010 | Anal Chem2010，82，： | 1 |
| 19 | Endoscopic and minimally invasive microsurgery approaches for treating brain tumor patients显示文摘 | Behnam B Nathaniel B Mark S | 2004 | J Neuro Oncol2004，69，3： | 1 |
| 20 | Skillful wintertime North American temperature forecasts out to 4 weeks based on the state of ENSO and the MJO显示文摘 | Nathaniel C J Dan C C Steven B F | 2014 | Weather and Forecasting2014，29，1： | 1 |
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