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| 1 | Structure-based Comparative Analysis and Prediction of N-linked Glycosylation Sites in Evolutionarily Distant Eukaryotes显示文摘The asparagine-X-serine/threonine (NXS/T) motif, where X is any amino acid except proline, is the consensus motif for N-linked glycosylation. Significant numbers of high-resolution crystal structures of glycosylated proteins allow us to carry out structural analysis of the N-linked glycosylation sites (NGS). Our analysis shows that there is enough structural information from diverse glycoproteins to allow the development of rules which can be used to predict NGS. A Python-based tool was developed to investigate asparagines implicated in N-glycosylation in five species: Homo sapiens, Mus musculus, Drosophila melanogaster, Arabidopsis thaliana and Saccharomyces cerevisiae. Our analysis shows that 78% of all asparagines of NXS/T motif involved in N-glycosylation are localized in the loop/turn conformation in the human proteome. Similar distribution was revealed for all the other species examined. Comparative analysis of the occurrence of NXS/T motifs not known to be glycosylated and their reverse sequence (S/TXN) shows a similar distribution across the secondary structural elements, indicating that the NXS/T motif in itself is not biologically relevant. Based on our analysis, we have defined rules to determine NGS. Using machine learning methods based on these rules we can predict with 93% accuracy if a particular site will be glycosylated. If structural information is not available the tool uses structural prediction results resulting in 74% accuracy. The tool was used to identify glycosylation sites in 108 human proteins with structures and 2247 proteins without structures that have acquired NXS/T site/s due to non-synonymous variation. The tool, Structure Feature Analysis Tool (SFAT), is freely available to the public at http://gffzzd6cb8b41b64c4e07hbxpxpcpwqfkp60w9.ffgz.tsg.suse.edu.cn/tools/sfat. | Phuc Vinh Nguyen Lam Radoslav Goldman Konstantinos Karagiannis Tejas Narsule Vahan Simonyan Valerii Soika Raja Mazumder | 2013 | Genomics, Proteomics & Bioinformatics2013，11，2： | 8 |
| 2 | Endoscopic fundoplication for the treatment of gastroesophageal reflux disease: Initial experience显示文摘 | Chaitan K. Narsule Miguel A. Burch Michael I. Ebright Donald T. Hess Roberto Rivas Benedict D.T. Daly Hiran C. Fernando | 2012 | The Journal of Thoracic and Cardiovascular Surgery2012，，1： | 1 |
| 3 | Sublobar versuslobar resection:current status 显示文摘 | Narsule C K Ebright MI Fernando HC | 2011 | Cancer J2011，17，1： | 1 |
| 4 | Endoscopic fundopli- cation for the treatment of gastroesophageal reflux disease: ini- tial experience 显示文摘 | Narsule CK Burch MA Ebright MI | 2012 | J Thorac Cardiovasc Surg2012，143，1： | 1 |
| 5 | The efficacy of electromagnetic navigation to assist with computed tomography-guided percutaneous thermal ablation of lung tumors显示文摘 | Narsule CK Sales Dos Santos R Gupta A | | 0，，： | 1 |
| 6 | Effect of delay in presentation on rate of perforation in children with ap- pendicitis显示文摘 | Narsule CK Kahle E J Kim DS | 2011 | Am J Emerg Med2011，29，8： | 1 |
| 7 | Effect of delay in presenta- tion on rate of perforation in children with appendicitis 显示文摘 | Narsule CK Kable EJ Kim DS | 2011 | Am J Emerg Med2011，29，8： | 1 |
| 8 | Sublobar versus lobar re-section:current status显示文摘 | Narsule CK Ebright MI Fernando HC | 2011 | Cancer J2011，17，1： | 1 |
| 9 | Effect of delay in presentation on rate of perforation in children with appendicitis显示文摘 | Narsule CK Kahle E J Kim DS | 2011 | Am J Emerg Med2011，29，8： | 1 |
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